BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,399,779	A→C	L67R (CTG→CGG)	mreC ←	cell wall structural complex MreBCD transmembrane component MreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,399,779	0	A	C	99.1%	386.0 / ‑3.2	116	L67R (CTG→CGG)	mreC	cell wall structural complex MreBCD transmembrane component MreC
Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (61/53); total (62/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.67e-01

Only 100 of 117 total aligned reads displayed.

ACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGT  >  NC_000913/3399545‑3400014

aCGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCcgcg                                                                                                                                                                                                                                          >  2:161655/1‑238 (MQ=255)

     aTTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCg                                                                                                                                                                                                                                    >  1:923042/1‑239 (MQ=255)

     aTTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCg                                                                                                                                                                                                                                    <  1:545448/239‑1 (MQ=255)

     aTTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCg                                                                                                                                                                                                                                    <  2:1158159/239‑1 (MQ=255)

      ttAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGa                                                                                                                                                                                                                                   >  1:1101885/1‑239 (MQ=255)

              aCCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATCCGCCa                                                                                                                                                                                                                            >  1:1030576/1‑238 (MQ=255)

                         tAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTc                                                                                                                                                                                                                 >  1:699011/1‑238 (MQ=255)

                         tAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTc                                                                                                                                                                                                                 >  2:86734/1‑238 (MQ=255)

                          aacaacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCAc                                                                                                                                                                                                               >  1:1023695/1‑239 (MQ=255)

                               cTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACg                                                                                                                                                                                                              >  2:248027/1‑235 (MQ=255)

                                  gATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACg                                                                                                                                                                                                              >  2:190644/1‑232 (MQ=255)

                                  gATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACg                                                                                                                                                                                                              <  1:190644/232‑1 (MQ=255)

                                   aTCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCCGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACgagga                                                                                                                                                                                                          <  2:238622/235‑1 (MQ=255)

                                   aTCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGc                                                                                                                                                                                                                                           >  2:273192/1‑202 (MQ=255)

                                   aTCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGc                                                                                                                                                                                                                                           <  1:273192/202‑1 (MQ=255)

                                    tCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCAtt                                                                                                                                                                                                     <  1:1146405/239‑1 (MQ=255)

                                           aaGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACa                                                                                                                                                                                              <  2:1005631/239‑1 (MQ=255)

                                                 cGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACg                                                                                                                                                                                                              >  1:1023402/1‑217 (MQ=255)

                                                 cGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACg                                                                                                                                                                                                              <  2:1023402/217‑1 (MQ=255)

                                                  gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCCGCAATTCACGAGGAGCATTGGAAACAAAGTAGa                                                                                                                                                                                       >  1:516445/1‑239 (MQ=255)

                                                  gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGa                                                                                                                                                                                       >  1:996912/1‑239 (MQ=255)

                                                  gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGa                                                                                                                                                                                       >  2:1061690/1‑239 (MQ=255)

                                                  gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGa                                                                                                                                                                                       >  2:361560/1‑239 (MQ=255)

                                                  gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGa                                                                                                                                                                                       >  1:329892/1‑239 (MQ=255)

                                                    tAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGaaa                                                                                                                                                                                     >  2:572905/1‑239 (MQ=255)

                                                         gTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACg                                                                                                                                                                                                              <  1:1001137/209‑1 (MQ=255)

                                                         gTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAgg                                                                                                                                                                                   >  2:559921/1‑236 (MQ=255)

                                                            gagaTAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGa                                                                                                                                                                              <  1:737900/238‑1 (MQ=255)

                                                                tAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCATCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGAcgg                                                                                                                                                                           <  1:304059/237‑1 (MQ=255)

                                                                 aaCCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGAcg                                                                                                                                                                            >  1:823301/1‑235 (MQ=255)

                                                                  aCCTGAGTCACCGTTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGt                                                                                                                                                                       <  1:338369/239‑1 (MQ=255)

                                                                  aCCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGt                                                                                                                                                                       <  2:616235/239‑1 (MQ=255)

                                                                    cTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGt                                                                                                                                                                       <  2:1030576/237‑1 (MQ=255)

                                                                    cTGAGTCACCATTTTCTGCTAGACCTGGCGCAGCGGGGAACCCAGCAGCTCGTGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTAt                                                                                                                                                                     <  2:1077218/239‑1 (MQ=255)

                                                                       aGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGCGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCa                                                                                                                                                                  >  1:586564/1‑239 (MQ=255)

                                                                       aGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCa                                                                                                                                                                  >  1:1047195/1‑239 (MQ=255)

                                                                       aGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCa                                                                                                                                                                  >  2:357591/1‑239 (MQ=255)

                                                                              aTTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGCCGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGt                                                                                                                                                           <  1:357591/239‑1 (MQ=255)

                                                                                      cTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGaaa                                                                                                                                                                                                <  1:976771/194‑1 (MQ=255)

                                                                                      cTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGaaa                                                                                                                                                                                                >  2:976771/1‑194 (MQ=255)

                                                                                      cTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGAttt                                                                                                                                                   >  1:655231/1‑239 (MQ=255)

                                                                                          tCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTg                                                                                                                                                  >  1:482631/1‑236 (MQ=255)

                                                                                                   aGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGAcggcg                                                                                                                                                                         >  1:190262/1‑204 (MQ=255)

                                                                                                     cGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCatat                                                                                                                                                               >  2:708101/1‑212 (MQ=255)

                                                                                                     cGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCa                                                                                                                                    >  1:129408/1‑239 (MQ=255)

                                                                                                     cGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGAACGGATTTGCCGGAACGTccccc                                                                                                                                    >  2:689426/1‑238 (MQ=255)

                                                                                                               agcagcTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGGATCCATATAAGTACGGATTTGACTGAACGTCCCCAggcg                                                                                                                                >  1:169481/1‑233 (MQ=255)

                                                                                                                 cagcTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGaaa                                                                                                                                                                                                <  2:570475/167‑1 (MQ=255)

                                                                                                                 cagcTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGaaa                                                                                                                                                                                                >  1:570475/1‑167 (MQ=255)

                                                                                                                    cTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCaa                                                                                                                     >  1:721770/1‑239 (MQ=255)

                                                                                                                     tgccgcAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGc                                                                                                                       <  1:769708/233‑1 (MQ=255)

                                                                                                                     tCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGc                                                                                                                       <  1:738873/236‑1 (MQ=255)

                                                                                                                      cgcgCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCaata                                                                                                                   >  1:1103556/1‑239 (MQ=255)

                                                                                                                             aCGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGc                                                                                                            <  2:1047195/239‑1 (MQ=255)

                                                                                                                                   gTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAgc                                                                                                       <  1:164548/238‑1 (MQ=255)

                                                                                                                                   gTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAgc                                                                                                       <  1:559921/238‑1 (MQ=255)

                                                                                                                                               gTATTGTCCAAGCTTCAGCATTTCACTTTTTTTCAGCAACAGTTCCGGACGTAACGCCCGGTTTTCAATTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCg                                                                                          <  2:482631/239‑1 (MQ=255)

                                                                                                                                                 aTTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGcc                                                                                        >  1:372161/1‑239 (MQ=255)

                                                                                                                                                     tCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCt                                                                                                                             <  2:736378/198‑1 (MQ=255)

                                                                                                                                                     tCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCt                                                                                                                             >  1:736378/1‑198 (MQ=255)

                                                                                                                                                      ccAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACg                                                                                                                                                                                                                               <  1:668499/99‑1 (MQ=255)

                                                                                                                                                      ccAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACg                                                                                                                                                                                                                               >  2:668499/1‑99 (MQ=255)

                                                                                                                                                       cAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGCCGTAACGCCCGGTGTTCAAGTTCTAATTGGCCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATa                                                                                  <  1:473857/239‑1 (MQ=255)

                                                                                                                                                           ctacggcagTTCACTGTTTTTCAGCAGCAGTTCCGGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGGCCCCAGGCGGCTGTCGGAAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATaa                                                                                 <  2:1111862/231‑1 (MQ=255)

                                                                                                                                                            aTCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAgg                                                                               <  1:86734/237‑1 (MQ=255)

                                                                                                                                                               agcagTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACg                                                                                                                                                                                                              <  2:689139/107‑1 (MQ=255)

                                                                                                                                                               agcagTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACg                                                                                                                                                                                                              >  1:689139/1‑107 (MQ=255)

                                                                                                                                                               agcagTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGAcgg                                                                                                                                                                           >  1:815174/1‑142 (MQ=255)

                                                                                                                                                               agcagTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGt                                                                                                                                                                       <  1:619753/146‑1 (MQ=255)

                                                                                                                                                               agcagTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGt                                                                                                                                                                       >  2:619753/1‑146 (MQ=255)

                                                                                                                                                                    ttCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTa                                                                     >  1:797365/1‑239 (MQ=255)

                                                                                                                                                                      cACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCCCCAGCATAAGGCGAATCTGTAGc                                                                   >  1:949041/1‑239 (MQ=255)

                                                                                                                                                                        cggTTTTCCATCAACGTTCCCTGACGAAACGCCCGGTTTTCAAGTGCTAAGTGGTCAGGCGAGGCCCGGGTTGGCGATCCGCCATCCAGCAATTCACGAGGGCCATTGGAAACAAGTTGGAAAGGACGGACGGCGGTATCCTTATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTGGGCAATAATAATGCCGCGGGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGa                                                                 <  2:916044/237‑1 (MQ=255)

                                                                                                                                                                         tGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACg                                                                                                                                                                                                                               <  2:351446/80‑1 (MQ=255)

                                                                                                                                                                           tttttCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACgg                                                              >  2:340480/1‑239 (MQ=255)

                                                                                                                                                                                     aCAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTa                                                    >  1:433536/1‑239 (MQ=255)

                                                                                                                                                                                                   accGCCCGGTTTTCAAGTTCTAATTGGTCAAGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCAt                                      <  1:738497/237‑1 (MQ=255)

                                                                                                                                                                                                   aaCGCCCGGTTTTCAATTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCa                                       <  1:1061690/238‑1 (MQ=255)

                                                                                                                                                                                                   aaCGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCAt                                      >  2:304738/1‑239 (MQ=255)

                                                                                                                                                                                                   aaCGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCAt                                      >  2:239276/1‑239 (MQ=255)

                                                                                                                                                                                                   aaCGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCAt                                      >  1:1148511/1‑239 (MQ=255)

                                                                                                                                                                                                             tttCAAGTTGTAATTGGTCACGCGGGGCCCGCGTCTGCGATACTGCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGCCGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCTGTTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGc                             <  2:1146578/238‑1 (MQ=255)

                                                                                                                                                                                                             tttCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCg                            <  1:304738/239‑1 (MQ=255)

                                                                                                                                                                                                              ttCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACGGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGt                           <  1:361560/239‑1 (MQ=255)

                                                                                                                                                                                                                  aGTTCTAATTGGTCCGGCGAGGCCCGCGTCTGCGATCCGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATtc                       <  2:169481/239‑1 (MQ=255)

                                                                                                                                                                                                                      cTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGt                   >  2:217397/1‑239 (MQ=255)

                                                                                                                                                                                                                      cTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGt                   >  2:732102/1‑239 (MQ=255)

                                                                                                                                                                                                                      cTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGCATCCCTATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGt                   >  1:530493/1‑239 (MQ=255)

                                                                                                                                                                                                                        aaTTGGTCACGCGAGGCCCGGGTCTGGGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGCCTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTAt                 <  2:923042/239‑1 (MQ=255)

                                                                                                                                                                                                                        aaTTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTa                  >  1:324565/1‑238 (MQ=255)

                                                                                                                                                                                                                        aaTTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTAt                 <  1:109920/239‑1 (MQ=255)

                                                                                                                                                                                                                        aaTTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTAt                 <  1:967754/239‑1 (MQ=255)

                                                                                                                                                                                                                         aTTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGt                                                                                                                                                           >  2:61859/1‑100 (MQ=255)

                                                                                                                                                                                                                         aTTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATc                >  1:991036/1‑239 (MQ=255)

                                                                                                                                                                                                                               cACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACca          >  1:637107/1‑239 (MQ=255)

                                                                                                                                                                                                                                   cgAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCata                                                                                                                                                                <  1:823134/85‑1 (MQ=255)

                                                                                                                                                                                                                                   cgAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCata                                                                                                                                                                >  2:823134/1‑85 (MQ=255)

                                                                                                                                                                                                                                      ggCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACcaggcagg    <  1:239276/238‑1 (MQ=255)

                                                                                                                                                                                                                                      ggCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACcaggcagg    <  1:217397/238‑1 (MQ=255)

                                                                                                                                                                                                                                       gCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAAAATAAGGCGAATCTGTAGCGACTGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCCCGTATACAACCAGGCAgggg  >  2:1113558/1‑238 (MQ=255)

ACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGT  >  NC_000913/3399545‑3400014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`