Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,571,007	T→G	K105Q (AAA→CAA)	glgX ←	glycogen debranching enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,571,007	0	T	G	100.0%	188.3 / NA	56	K105Q (AAA→CAA)	glgX	glycogen debranching enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/31);  total (25/31)

CCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCATAGCGATGTTCCTGGCCATTGGCGTCAAAGACACACAGTTCTACCCGCTCGGCATGA  >  NC_000913/3570773‑3571236                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        ccGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGGTGTTGCGATAGTCAGGTTCATTATGACCGGCGGGCCGCAGCGGGTTATCTTGaaa                                                                                                                                                                                                                                      >  2:659682/1‑238 (MQ=255)                       aGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCgcgcgc                                                                                                                                                                                                               <  2:987376/239‑1 (MQ=255)                        gTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCa                                                                                                                                                                                                              >  2:703194/1‑239 (MQ=255)                          gCCACGGTTCTCGACCGGGATGTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTTCGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAg                                                                                                                                                                                                            <  1:1166648/239‑1 (MQ=255)                          gCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCa                                                                                                                                                                                                              <  2:1157564/237‑1 (MQ=255)                          gCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCa                                                                                                                                                                                                              <  1:313157/237‑1 (MQ=255)                           ccACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCCGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCCCGCAAgg                                                                                                                                                                                                           >  2:410660/1‑239 (MQ=255)                           ccACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAg                                                                                                                                                                                                            <  2:150313/238‑1 (MQ=255)                           ccACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGGTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAgg                                                                                                                                                                                                           >  1:980289/1‑239 (MQ=255)                                              tctcCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGc                                                                                                                                                                                         <  1:54382/238‑1 (MQ=255)                                                      tGCAAGTACGTTAACCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGc                                                                                                                                                                                         <  1:967737/230‑1 (MQ=255)                                                        cAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGGTCAATCAACAAATTCGCCGGGGTAAAGc                                                                                                                                                                              >  1:126483/1‑239 (MQ=255)                                                            tACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGAt                                                                                                                                                                           >  1:61091/1‑238 (MQ=255)                                                                ttAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTTCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTTCCGCGCGCAAGGATCAAACAACAACTTCTCCGGGTTAAAGCGATGcgc                                                                                                                                                                       >  2:46156/1‑236 (MQ=255)                                                                ttAAACCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGCCAATTTGCCGCGCGCAAGGAACAATCACACACTTCGCCCGGTTaaaa                                                                                                                                                                               >  2:967737/1‑229 (MQ=255)                                                                              tGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATg                                                                                                                                                                          >  1:157020/1‑221 (MQ=255)                                                                              tGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATg                                                                                                                                                                          <  2:157020/221‑1 (MQ=255)                                                                                        aaaTGATGGTGCTGCCCCACGGCGTGCCCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGa                                                                                                                                             <  2:802361/239‑1 (MQ=255)                                                                                         aaTGAGGGTGCTGCCCCGGGGCGTGCGCGGCGGGTCATCATCTTCCCAGGCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTGGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGaa                                                                                                                                            <  1:677230/239‑1 (MQ=255)                                                                                               ggTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑At                                                                                                                                     >  1:1121237/1‑239 (MQ=255)                                                                                                 tgctgcCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCg                                                                                                                                                                                                                               <  2:471345/149‑1 (MQ=255)                                                                                                 tgctgcCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCg                                                                                                                                                                                                                               >  1:471345/1‑149 (MQ=255)                                                                                                                tGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGGT‑GCCACGGGCCCAGAAAGCG‑GTAAAACAAACACAAAccc                                                                                                                    >  2:88773/1‑239 (MQ=255)                                                                                                                  cgcgGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGAAGCCCCTCGGCGGGTTGGCCGGGGGCCCAG‑ACGCG‑ATAAACATAAATCAAAcccag                                                                                                                  >  2:661511/1‑237 (MQ=255)                                                                                                                                         gTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAAAGCAAACCCCGGCGCGCCTCCGGCAGACAACCgg                                                                                           >  1:298955/1‑238 (MQ=255)                                                                                                                                           cATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGGT‑GCCAGGGGGCCAGAACGCGAAAAACCAAAAC‑CAAACCCCGGCGCCCACCCCGCCGATaaacctc                                                                                          >  2:1041847/1‑233 (MQ=255)                                                                                                                                            aTAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGGT‑GGCAGGGGCCCTGCACGCG‑ATCACCATAACGCAAACCCGGGCGCGCATCACGCAGAAAAACgcgc                                                                                         >  2:146147/1‑238 (MQ=255)                                                                                                                                                tGATCAACAACCACTGCGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCa                                                                                                                                <  1:1135387/195‑1 (MQ=255)                                                                                                                                                    cAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGt                                                                                                                                                                                    <  2:455286/141‑1 (MQ=255)                                                                                                                                                    cAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGt                                                                                                                                                                                    >  1:455286/1‑141 (MQ=255)                                                                                                                                                      accaccCCTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGcc                                                                              <  1:661511/239‑1 (MQ=255)                                                                                                                                                       ccaccaCTACGCATTGCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCa                                                                             <  1:88773/239‑1 (MQ=255)                                                                                                                                                          ccacTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTcgg                                                                                                                                                                  >  1:317765/1‑153 (MQ=255)                                                                                                                                                          ccacTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTcgg                                                                                                                                                                  <  2:317765/153‑1 (MQ=255)                                                                                                                                                          ccacTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGGT‑GCCCGGGGGCCCGAACCCG‑AGAACCATAACCCCAAc                                                                                                                      >  2:1135387/1‑195 (MQ=255)                                                                                                                                                                cGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAg                                                                                                                                                                               >  1:347619/1‑134 (MQ=255)                                                                                                                                                                cGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAg                                                                                                                                                                               <  2:347619/134‑1 (MQ=255)                                                                                                                                                                     ttCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCCCTAACGCG‑ATAAACATAACCCCAACCCGGGCGCGCCACCGGCCGAGAACCGGGCCCAAAGGACCCACTGGGCCCTGGCaa                                                               >  2:728546/1‑239 (MQ=255)                                                                                                                                                                       cGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCa                                                                                                                                                                                                              >  1:946153/1‑96 (MQ=255)                                                                                                                                                                       cGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCa                                                                                                                                                                                                              <  2:946153/96‑1 (MQ=255)                                                                                                                                                                          cgcAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCa                                                           <  1:145423/238‑1 (MQ=255)                                                                                                                                                                               tGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCATAGCg                                                      <  2:360238/238‑1 (MQ=255)                                                                                                                                                                                            gcgATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCATAGCGATGTTCCTGGCCAt                                        <  2:267172/239‑1 (MQ=255)                                                                                                                                                                                              gATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCATAGCGATGTTCCTGGCCATTg                                      <  2:1121237/239‑1 (MQ=255)                                                                                                                                                                                                        ttCATTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCATAGCGATGTTCCTGGCCATTGGCGTCAAAg                             <  2:61091/238‑1 (MQ=255)                                                                                                                                                                                                           aTTATGACCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCATAGCGATGTTCCTGGCCATTGGCGTCAAAGacac                         <  1:659682/239‑1 (MQ=255)                                                                                                                                                                                                           aTTAGGACCGGCGTGCAGCAGCGGGTTATCTTGAAACGCCCCGGCAATGTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTG‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCATAGCGATGTTCCTGGCCATTGGc                                    <  1:302457/228‑1 (MQ=255)                                                                                                                                                                                                            ttagggccGGCGTGCAGTAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCATAGCGATGTTCCTGGCCATTGGCGTCAAAGacaca                        <  1:46156/233‑1 (MQ=255)                                                                                                                                                                                                            ttATGCCCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCATAGCGATGTTCCTGGCCATTGGCGTCAAAGacaca                        <  1:703194/239‑1 (MQ=255)                                                                                                                                                                                                             tATGCCCGGCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCATAGCGATGTTCCTGGCCATTGGCGTCAAAGacacac                       <  1:1041847/239‑1 (MQ=255)                                                                                                                                                                                                                     gCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑At                                                                                                                                     <  2:751322/121‑1 (MQ=255)                                                                                                                                                                                                                     gCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑At                                                                                                                                     >  1:751322/1‑121 (MQ=255)                                                                                                                                                                                                                     gCGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGGT‑GCCAGGGGCCATGAACGGG‑GTAACCATAACGCAAAACCGGGCGCGCATCCGGCCGATAACCGGGCCAAATGTCGCCCCCGTGCCCCGGGCAATCACAGCGATGTTCCTGGCCCTTGTGGTCCAAAacac                         >  2:302457/1‑229 (MQ=255)                                                                                                                                                                                                                      cGTGCAGCAGCGGGTTATCTTGAAACTCCCCGTCAATTTg                                                                                                                                                                                                                       >  2:138410/1‑40 (MQ=255)                                                                                                                                                                                                                                   gTTATCGTGAAACTCCCCGGCAATTTGCCGGGCGCAAGGGTCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCATAGCGATGTTCCTGGCCATTGGCGTCAAAGACACACAGTTCTACCCGCTCGGCATGa  <  1:146147/238‑1 (MQ=255)                                                                                                                                                                                                                                       tCTTGAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCATAGCg                                                      <  1:25710/182‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        CCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTCAATTTGCCGCGCGCAAGGATCAATCAACAACTTCGCCGGGTTAAAGCGATGCCCCTCGGCGGGTT‑GCCAGGGGCCATGAACGCG‑ATAACCATAACGCAAACCCGGGCGCGCATCCGGCAGATAACCGTGCCAAATGTCGCCACTGTGCCCTGGCAAGTCATAGCGATGTTCCTGGCCATTGGCGTCAAAGACACACAGTTCTACCCGCTCGGCATGA  >  NC_000913/3570773‑3571236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑