Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,616,561	T→G	V127G (GTT→GGT)	nikC →	nickel ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,616,561	0	T	G	100.0%	186.7 / NA	51	V127G (GTT→GGT)	nikC	nickel ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/34);  total (17/34)

TGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGATATCGGCCATATGAT  >  NC_000913/3616328‑3616792                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        tGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGc                                                                                                                                                                                                                                     <  1:706048/238‑1 (MQ=255)                     ccGATCCCGTAGGTCGCGGTATTTTCGCGCGGCTGATGGCAGCGGCCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGAcc                                                                                                                                                                                                                <  2:666338/239‑1 (MQ=255)                                    gcgATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGGTGATCAGGCCACCCTGCGGGCCGCCGCGATGTTTATTACCCTCCCGCACCCGGTTCTGTCGGTCTTTATGGGGTGCGTGCGCGGGACCCGGCCGGCCCACgctaatattgc                                                                                                                                                                                                  >  2:32108/1‑228 (MQ=255)                                          ttttCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                                                                                                                                                                           <  2:1018411/239‑1 (MQ=255)                                                cgcgGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGGGCGTTGATCAAGGCACCCTGCGCGCCGCCGATATGTTTATGACCTTCCCGACCTCGATTTTGTCGTTCTTTTTGGGTGGGGGGCTCGGCACCGGGCTGACCCACGCAAATtttgccatcgc                                                                                                                                                                                            >  2:756504/1‑232 (MQ=255)                                                   ggCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCa                                                                                                                                                                                   <  1:527704/238‑1 (MQ=255)                                                   ggCGGATGGCGGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCa                                                                                                                                                                                   <  1:505670/238‑1 (MQ=255)                                                     cTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCa                                                                                                                                                                                                                           <  1:496045/196‑1 (MQ=255)                                                     cTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACt                                                                                                                                                                                 <  1:756504/238‑1 (MQ=255)                                                       gATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTggg                                                                                                                                                                              <  1:139285/239‑1 (MQ=255)                                                        aTGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGc                                                                                                                                                                                                                                     <  1:290453/183‑1 (MQ=255)                                                                   cggcgTGTCGC‑CGGGTCGGGAAGGGGCGTGCTGCTTCGTGTGCTGACATTAGGGTTGGTTATTGGCGGCAGGGCTGGGTTGATTGGCGGGCGCGTTGATCAGACCACCTTGCGCGGCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCAc                                                                                                                                                                  <  2:900623/236‑1 (MQ=255)                                                                       gtgtCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGTCATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCa                                                                                                                                                               <  1:193550/238‑1 (MQ=255)                                                                       gtgtCGCTCGGTTCGGTAATGGCCGGCCGGCTGCTGGTGCTGACATTAGGGCTGGTTAGTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCAt                                                                                                                                                              <  2:845398/239‑1 (MQ=255)                                                                             cTCGGTTCAGTAATGGCCTGCCCGCTGCTGGGGCTGGCATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTgcg                                                                                                                                                        <  2:253714/239‑1 (MQ=255)                                                                                        aaTGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGa                                                                                                                                             <  2:244116/239‑1 (MQ=255)                                                                                           ggCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGAttt                                                                                                                                          <  1:32108/239‑1 (MQ=255)                                                                                                 cctgcTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGGTGATCCGGCCACCCTGCGCGGCGCCGAGATGTTTATGACCTTCCCCACCTCGATTCTGGCGCTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAAAGTAATTATCGCCCTCGCCCCGTTGCACTGGGCGGGGGATGCACCCCTGGTGCGCAGCCTCGTGGttttccta                                                                                                                                     >  2:809853/1‑233 (MQ=255)                                                                                                                   aTTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGGTCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTtgtg                                                                                                                   <  1:594038/238‑1 (MQ=255)                                                                                                                     tAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGGTCAGGCCACCATGCGCGCCGCCCGTATGTTTATGACCTTCCCGCCCTTGATTCTGTCGTTTTTTTTGGGGGGGGTGCTCGGCCCCCGGCCTACCAACGGAAATATCGCCATCCCCCTGTCCCACTGGGCGCGGGTTGCCCGCATGGTGCGCAGCCTGGTGATTTCACCTCGCCCACCCGAGTTTTTGCtt                                                                                                                >  2:59843/1‑238 (MQ=255)                                                                                                                      aGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGGTGATCAGGCCACCATGCGCGGCGCCGATATGGTTTTGACCTTTCCGACCTCGATTCTGTCGGTCTTTATGGGGGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATTGCCATCCCCCTGTCGCACTTGGCGCGGTATGCACGCATGGTGGGCAGCCTGGTGATTTTACCACGCCAAAGCGAGGTtgt                                                                                                                    >  2:594038/1‑234 (MQ=255)                                                                                                                           tGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGGTGATCAGGCCCCCCTGCGCGTCGCCGATATGTTTATGACCCTCCCGACCTCGGTTCTGTCGGTCTTTATGGGGGGCGTGCTCGGCACCGGGCTGACCCACGTAATTATCGCCCTCGCCCTGTCGCCCTGGGCGTGGGATGCACGCCTGGTGCGCCGCCTGGGGATTTCACTACCCCAACGCGAGCTTGTGCTGGGGTc                                                                                                           >  2:883948/1‑238 (MQ=255)                                                                                                                             gTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGc                                                                                                                                                                                                                                     <  2:433861/114‑1 (MQ=255)                                                                                                                             gTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGc                                                                                                                                                                                                                                     >  1:433861/1‑114 (MQ=255)                                                                                                                                            gcCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTg                                                                                             <  1:69500/235‑1 (MQ=255)                                                                                                                                             ccGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGCCCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCggg                                                                                         <  2:878971/238‑1 (MQ=255)                                                                                                                                              ggggTTGATTGGCGGGCGTGTTGATCGGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGcc                                                                                       <  1:809853/238‑1 (MQ=255)                                                                                                                                               gggTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTTTGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGa                                                                                                                                                                                                                  >  2:904832/1‑115 (MQ=255)                                                                                                                                               gggTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGa                                                                                                                                                                                                                  <  1:904832/115‑1 (MQ=255)                                                                                                                                                    gATTGGGGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCTATATGGTTATGGCCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCAtgtg                                                                                  <  1:867863/238‑1 (MQ=255)                                                                                                                                                           gggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGgtgt                                                                           <  1:603609/238‑1 (MQ=255)                                                                                                                                                                   tGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTATGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCATGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGat                                                                   <  1:962777/238‑1 (MQ=255)                                                                                                                                                                                      gTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGAt                                               <  1:1081452/239‑1 (MQ=255)                                                                                                                                                                                                       gACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACt                                                                                                                                                                                 >  2:629298/1‑92 (MQ=255)                                                                                                                                                                                                       gACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACt                                                                                                                                                                                 <  1:629298/92‑1 (MQ=255)                                                                                                                                                                                                            tCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGAt                                               >  2:914941/1‑217 (MQ=255)                                                                                                                                                                                                            tCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGAt                                               <  1:914941/217‑1 (MQ=255)                                                                                                                                                                                                            tCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCa                         >  2:560555/1‑239 (MQ=255)                                                                                                                                                                                                            tCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCa                         >  2:646800/1‑239 (MQ=255)                                                                                                                                                                                                            tCCCGACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCa                         >  1:203891/1‑239 (MQ=255)                                                                                                                                                                                                                gACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGc                     <  1:648444/239‑1 (MQ=255)                                                                                                                                                                                                                gACCTCGATTCTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGc                     <  1:560555/239‑1 (MQ=255)                                                                                                                                                                                                                      gATTCTGTCGTTCGTTATGGGTGGCGTGCTCGGCCGCGGGCTGTCCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTCTGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGata               <  2:668948/239‑1 (MQ=255)                                                                                                                                                                                                                      caTTCTGTCGTTCTTTGTGGGGGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTGCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGata               <  1:198873/238‑1 (MQ=255)                                                                                                                                                                                                                          cTGTCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGATATCgg           <  1:646800/239‑1 (MQ=255)                                                                                                                                                                                                                             tCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCAtgtg                                                                                  <  2:188504/165‑1 (MQ=255)                                                                                                                                                                                                                             tCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCAtgtg                                                                                  >  1:188504/1‑165 (MQ=255)                                                                                                                                                                                                                             tCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGATATCGGCCa        >  2:1093616/1‑239 (MQ=255)                                                                                                                                                                                                                             tCGTTCTTTATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGATATCGGCCa        >  2:1040181/1‑239 (MQ=255)                                                                                                                                                                                                                                ttCTTTATGGGTGGCGTGCTCGGCACCGGGCTGAc                                                                                                                                                                                                                 <  1:189685/35‑1 (MQ=255)                                                                                                                                                                                                                                    ttATGGGTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGATATCGGCCATatgat  >  2:56025/1‑238 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        TGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGATATCGGCCATATGAT  >  NC_000913/3616328‑3616792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑