Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,749,326	A→C	D32A (GAT→GCT)	sgbU →	putative L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,749,326	0	A	C	100.0%	207.8 / NA	58	D32A (GAT→GCT)	sgbU	putative L‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (29/29);  total (29/29)

TTACCCCTGCTGACCTGCCGCTGTTTAAAGATATCCGCGTGAAAGCGTTTATTGCCGGGCGGGCACTGGCAGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGC  >  NC_000913/3749091‑3749555                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      ttcccccTGCTGACCTGCCGCTGTTTAAAGATATCCGCGTGAAAGCGTTTATTGCCGGGCGGGCACTGGCAGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCttt                                                                                                                                                                                                                                    <  2:121760/236‑1 (MQ=255)         gctgACCTGCCGCTGTTTAAAGATATCCGCGTGAAAGCGTTTATTGCCGGGCGGGCACTGGCAGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGGGCGC‑‑GTGCGTAATCCTCAGGTAGGGATTTATGAAAAAGCGCTGGCGGAAGATCTCTCCTGGGCGGAGCGGCTGGGGCTGGCAAAAAACGTCGGGTTTTCTTTTGTCTaaa                                                                                                                                                                                                                            >  2:708728/1‑239 (MQ=255)                          tAAAGATATCCGCGTGAAAGCGTTTATTGCCGGGCGGGCACTGGCAGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGGGCTGGCAAAAAGCTGCGGGTTTGCTTTTTTCGAAATGTCGGGGGGTGaaaac                                                                                                                                                                                                           >  1:649691/1‑237 (MQ=255)                                  tCCGCGTGAAAGCGTTTATTGCCGGGCGGGCACTGGCAGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GGGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGGGCTGGCAAAAACCTGCGGGTTTGCTTTTTTCGAAAAGTCGGTGGGTGGAAccaccgcacg                                                                                                                                                                                                   >  2:1161989/1‑231 (MQ=255)                                  tCCGCGTGAAAGCGTTTATTGCCGGGCGGGCACTGGCAGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGGGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGGGCTGGGCAAAAGCTGCGGGTTTGCTTTTTGTGAAACGTCGGTGGGTGAAAAcgacgacca                                                                                                                                                                                                   >  2:946677/1‑236 (MQ=255)                                      cgTGAAAGCGTTTATTGCCGGGCGGGCACTGGCAGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGGTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGGCGAACGGct                                                                                                                                                                                                >  1:1058196/1‑238 (MQ=255)                                          aaaGCGTTTATTGCCGGGCGGGCACTGGCAGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCAc                                                                                                                                                                                           >  1:843143/1‑239 (MQ=255)                                                        cgggcgggCACTGGCCGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAg                                                                                                                                                                              <  2:856218/238‑1 (MQ=255)                                                         gggcgggcACTGGCAGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGaa                                                                                                                                                                                                                             <  2:7545/190‑1 (MQ=255)                                                         gggcgggcACTGGCAGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGaa                                                                                                                                                                                                                             >  1:7545/1‑190 (MQ=255)                                                                     gCAGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAgg                                                                                                                                                                 >  1:613100/1‑238 (MQ=255)                                                                          cgcgGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGa                                                                                                                                                                <  1:946677/234‑1 (MQ=255)                                                                                            aGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGTGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCgatgat                                                                                                                                          <  1:655384/238‑1 (MQ=255)                                                                                                 ctggcGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGaaa                                                                                                                                     <  1:247779/238‑1 (MQ=255)                                                                                                 ctggcGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGaaa                                                                                                                                     <  1:190483/238‑1 (MQ=255)                                                                                                 ctggcGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGaaa                                                                                                                                     <  1:182578/238‑1 (MQ=255)                                                                                                  tggcGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAAACGACGAACGGCCCTCACGTCTTGACTGGAGCGCCGCACAAAGGACCTCGCTGGTTGCCCCGATGATCGAAACa                                                                                                                                   >  2:700562/1‑239 (MQ=255)                                                                                                   ggcGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAg                                                                                                                                  <  1:892313/239‑1 (MQ=255)                                                                                                   ggcGATTTCCATGCGCAAATCGACGCGATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAg                                                                                                                                  <  2:574744/239‑1 (MQ=255)                                                                                                     cGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTg                                                                                                                                                                                 <  2:359146/190‑1 (MQ=255)                                                                                                     cGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTg                                                                                                                                                                                 >  1:359146/1‑190 (MQ=255)                                                                                                        tttCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGtt                                                                                                                             <  1:708728/239‑1 (MQ=255)                                                                                                                 gcAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATccc                                                                                                                     <  2:843143/238‑1 (MQ=255)                                                                                                                   aaaTCGACGCTATCTGGGGAGGTGCGG‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGt                                                                                                                   <  1:362806/238‑1 (MQ=255)                                                                                                                    aaTCGACGTTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCg                                                                                                                 <  2:727738/239‑1 (MQ=255)                                                                                                                    aaTCGACGCTATCTGGGGAGGTTCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCg                                                                                                                 <  1:282791/239‑1 (MQ=255)                                                                                                                    aaTCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCg                                                                                                                 <  2:613100/239‑1 (MQ=255)                                                                                                                          cGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCg                                                                                                                                                                                                                       >  2:839115/1‑131 (MQ=255)                                                                                                                          cGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCg                                                                                                                                                                                                                       <  1:839115/131‑1 (MQ=255)                                                                                                                           gCTATCTGGGGAGGTGCGCGTGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGAtgtg                                                                                                            <  1:112806/239‑1 (MQ=255)                                                                                                                                         tGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGCTGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGcac                                                                                                 >  2:845684/1‑234 (MQ=255)                                                                                                                                         tGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGa                                                                                                                         >  2:139496/1‑210 (MQ=255)                                                                                                                                         tGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGa                                                                                                                         <  1:139496/210‑1 (MQ=255)                                                                                                                                         tGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACAtcg                                                                                             <  1:259759/238‑1 (MQ=255)                                                                                                                                         tGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACAtcg                                                                                             >  1:719680/1‑238 (MQ=255)                                                                                                                                         tGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACAtcg                                                                                             >  2:259759/1‑238 (MQ=255)                                                                                                                                           cgc‑‑gTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCg                                                                                                                 >  1:1178210/1‑216 (MQ=255)                                                                                                                                           cgc‑‑gTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCg                                                                                                                 <  2:1178210/216‑1 (MQ=255)                                                                                                                                           cgc‑‑gTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGGGTTGGGAGCCCGTCGATGTGCCTGTCCGCAAAtcgtc                                                                                           >  1:818831/1‑238 (MQ=255)                                                                                                                                                     tAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGGTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGGTCCCGTCGATGTGCCTGTCCGCACAtcgtc                                                                                           >  1:691019/1‑230 (MQ=255)                                                                                                                                                                   ggATTTATGAAAAAGCGCTGGCGCAAGATCTCTCCTGGCCGGAGCGGGTGGTGCTGGCAAAAAGCTGCTGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCGCGTCTTGACTGGAGCGCCGCACAAAGGCCTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCt                                                                                        <  1:457276/219‑1 (MQ=255)                                                                                                                                                                   ggATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCtt                                                                                                                                                                                                                                     <  1:845546/78‑1 (MQ=255)                                                                                                                                                                   ggATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCtt                                                                                                                                                                                                                                     >  2:845546/1‑78 (MQ=255)                                                                                                                                                                         aTGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGGGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTTGTAGCCGTGACGGGGcc                                                              >  2:831449/1‑238 (MQ=255)                                                                                                                                                                             aaaaGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGGGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGGGGATGAAACCGACGAACGGCCCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGGTGCCGCGATGATCGAAACAGGGGTTGGGATCCCGGCGATGTGCCGGTCCGCACATCGGCGCTTTCCCTTTgg                                                                              >  2:457276/1‑219 (MQ=255)                                                                                                                                                                               aaGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTgcgc                                                        >  1:218386/1‑239 (MQ=255)                                                                                                                                                                                          aaaGATCTCTCCTGGCCGGGGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGgcgc                                              <  1:828372/238‑1 (MQ=255)                                                                                                                                                                                            aGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGGGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCggg                                           >  1:712472/1‑239 (MQ=255)                                                                                                                                                                                                   tcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCCCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGGGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCAt                                    >  1:166095/1‑239 (MQ=255)                                                                                                                                                                                                          cGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTaaa                             <  1:831449/239‑1 (MQ=255)                                                                                                                                                                                                             aGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGGGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCg                          >  1:648773/1‑239 (MQ=255)                                                                                                                                                                                                             aGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAAAGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCg                          >  1:401162/1‑239 (MQ=255)                                                                                                                                                                                                                  cTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAg                                                                                                                                                                              >  1:291519/1‑86 (MQ=255)                                                                                                                                                                                                                  cTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAg                                                                                                                                                                              <  2:291519/86‑1 (MQ=255)                                                                                                                                                                                                                           gCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGAtg                                                                                                              >  2:1164501/1‑141 (MQ=255)                                                                                                                                                                                                                           gCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGAtg                                                                                                              <  1:1164501/141‑1 (MQ=255)                                                                                                                                                                                                                                    tGCGGTTTTGCTTTTGTCGAAATGGCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGAtctc     <  2:648773/237‑1 (MQ=255)                                                                                                                                                                                                                                     gCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGc  >  1:657351/1‑239 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      TTACCCCTGCTGACCTGCCGCTGTTTAAAGATATCCGCGTGAAAGCGTTTATTGCCGGGCGGGCACTGGCAGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTATCTGGGGAGGTGCGC‑‑GTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGC  >  NC_000913/3749091‑3749555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑