Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,800,808 T→G D53A (GAT→GCT)  waaY ← lipopolysaccharide core biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,800,8080TG100.0% 293.2 / NA 77D53A (GAT→GCT) waaYlipopolysaccharide core biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/44);  total (33/44)

CAACTCTATGCCATCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAATCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTCCTGA  >  NC_000913/3800576‑3801036
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                    
caACTCTATGCCATCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTAt                                                                                                                                                                                                                                >  1:1137755/1‑239 (MQ=255)
   ctctATGCCATCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCaa                                                                                                                                                                                                                             >  1:267801/1‑239 (MQ=255)
   ctctATGCCATCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCaa                                                                                                                                                                                                                             >  2:1187949/1‑239 (MQ=255)
   ctctATGCCATCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCaa                                                                                                                                                                                                                             <  2:265691/239‑1 (MQ=255)
     ctATGCCATCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATa                                                                                                                                                                                                                           <  2:885126/239‑1 (MQ=255)
           cATCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCAt                                                                                                                                                                                                                      <  2:267801/238‑1 (MQ=255)
             tCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATa                                                                                                                                                                                                                     >  1:124437/1‑237 (MQ=255)
              cAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACt                                                                                                                                                                                                                  <  2:1137755/239‑1 (MQ=255)
                 tataCTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACtttt                                                                                                                                                                                                               <  1:359367/239‑1 (MQ=255)
                 tataCTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACtttt                                                                                                                                                                                                               <  2:124437/239‑1 (MQ=255)
                   taCTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAGCTTTTGt                                                                                                                                                                                                             <  1:1187949/239‑1 (MQ=255)
                      tCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTatc                                                                                                                                                                                                          >  1:484456/1‑239 (MQ=255)
                         atgatCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTatcatc                                                                                                                                                                                                       >  2:935472/1‑239 (MQ=255)
                           gatCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACtttt                                                                                                                                                                                                               <  1:752014/229‑1 (MQ=255)
                           gatCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACtttt                                                                                                                                                                                                               >  2:752014/1‑229 (MQ=255)
                                     taAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGaaa                                                                                                                                                                                           <  2:271886/239‑1 (MQ=255)
                                                  aaCGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTtga                                                                                                                                                                                   >  2:724343/1‑234 (MQ=255)
                                                  aaCGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTtga                                                                                                                                                                                   <  1:724343/234‑1 (MQ=255)
                                                  aaCGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGata                                                                                                                                                                              >  1:1062898/1‑239 (MQ=255)
                                                  aaCGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGata                                                                                                                                                                              >  1:30525/1‑239 (MQ=255)
                                                   aCGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTtgat                                                                                                                                                                                  <  2:30525/234‑1 (MQ=255)
                                                   aCGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTtgat                                                                                                                                                                                  <  1:218622/234‑1 (MQ=255)
                                                   aCGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGatat                                                                                                                                                                             <  2:683711/239‑1 (MQ=255)
                                                              tCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCAt                                                                                                                                                                                                                      >  1:1025227/1‑187 (MQ=255)
                                                              tCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCAt                                                                                                                                                                                                                      <  2:1025227/187‑1 (MQ=255)
                                                                  ttCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACa                                                                                                                                                              >  1:362533/1‑239 (MQ=255)
                                                                     aGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACaaga                                                                                                                                                           >  2:625606/1‑239 (MQ=255)
                                                                        cAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACaagaag                                                                                                                                                         <  2:484456/238‑1 (MQ=255)
                                                                                       aTTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGa                                                                                                                                         >  2:505272/1‑239 (MQ=255)
                                                                                          gAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTaa                                                                                                                                                 <  2:1051972/228‑1 (MQ=255)
                                                                                          gAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGt                                                                                                                                      <  1:610339/239‑1 (MQ=255)
                                                                                              gtATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACt                                                                                                                                   <  2:1062898/238‑1 (MQ=255)
                                                                                               tATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATa                                                                                                                                                                                                                     >  1:679894/1‑155 (MQ=255)
                                                                                               tATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATa                                                                                                                                                                                                                     <  2:679894/155‑1 (MQ=255)
                                                                                                     aCCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGa                                                                                                                                                                                                 <  2:826309/169‑1 (MQ=255)
                                                                                                     aCCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGa                                                                                                                                                                                                 >  1:826309/1‑169 (MQ=255)
                                                                                                        catCATTTCGTCCTTTTTGAGTTTGCNNNAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGttt                                                                                                                        <  1:856278/237‑1 (MQ=255)
                                                                                                         ttCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTtgat                                                                                                                                                                                  >  2:698964/1‑180 (MQ=255)
                                                                                                         ttCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTtgat                                                                                                                                                                                  <  1:698964/180‑1 (MQ=255)
                                                                                                         ttCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACaagaag                                                                                                                                                         >  1:887174/1‑205 (MQ=255)
                                                                                                         ttCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACaagaag                                                                                                                                                         <  2:887174/205‑1 (MQ=255)
                                                                                                         ttCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACaagaag                                                                                                                                                         <  2:473585/205‑1 (MQ=255)
                                                                                                         ttCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACaagaag                                                                                                                                                         >  1:473585/1‑205 (MQ=255)
                                                                                                          tCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTc                                                                                                                      >  2:277509/1‑239 (MQ=255)
                                                                                                           cATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAAcc                                                                                                                                                                                       >  1:1130416/1‑173 (MQ=255)
                                                                                                           cATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAAcc                                                                                                                                                                                       <  2:1130416/173‑1 (MQ=255)
                                                                                                           cATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCa                                                                                                                     >  1:867716/1‑239 (MQ=255)
                                                                                                                       tttGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCa                                                                                                                                                                                                                              <  1:353003/122‑1 (MQ=255)
                                                                                                                       tttGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCa                                                                                                                                                                                                                              >  2:353003/1‑122 (MQ=255)
                                                                                                                             ttttCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACATCCaa                                                                                                    <  1:560819/238‑1 (MQ=255)
                                                                                                                             tttGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCaa                                                                                                    <  2:362533/238‑1 (MQ=255)
                                                                                                                               tGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATtta                                                                                                                                                                          >  1:124004/1‑166 (MQ=255)
                                                                                                                               tGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATtta                                                                                                                                                                          <  2:124004/166‑1 (MQ=255)
                                                                                                                                 cTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACaagaag                                                                                                                                                         <  2:254479/181‑1 (MQ=255)
                                                                                                                                 cTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACaagaag                                                                                                                                                         >  1:254479/1‑181 (MQ=255)
                                                                                                                                  tCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCtt                                                                                               <  1:277509/238‑1 (MQ=255)
                                                                                                                                  tCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCtt                                                                                               <  1:505272/238‑1 (MQ=255)
                                                                                                                                           cGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAAACTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAACACAACCAAGTCTTTGATCTTGct                                                                                     >  2:800906/1‑239 (MQ=255)
                                                                                                                                                            ttttAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAAt                                                                                                                                                                                                                            <  2:167749/87‑1 (MQ=255)
                                                                                                                                                            ttttAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAAt                                                                                                                                                                                                                            >  1:167749/1‑87 (MQ=255)
                                                                                                                                                                     agaCTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCAt                                                                                                                                                                                                                      <  1:2155/84‑1 (MQ=255)
                                                                                                                                                                     agaCTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCAt                                                                                                                                                                                                                      >  2:2155/1‑84 (MQ=255)
                                                                                                                                                                     agaCTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATa                                                                                                                                               >  2:119121/1‑155 (MQ=255)
                                                                                                                                                                     agaCTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATa                                                                                                                                               <  1:119121/155‑1 (MQ=255)
                                                                                                                                                                     agaCTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAAt                                                                            <  2:210584/222‑1 (MQ=255)
                                                                                                                                                                     agaCTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAAt                                                                            >  1:210584/1‑222 (MQ=255)
                                                                                                                                                                      gaCTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTaa                                                           <  2:972505/238‑1 (MQ=255)
                                                                                                                                                                           aaaGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTtgat                                                                                                                                                                                  <  1:979959/114‑1 (MQ=255)
                                                                                                                                                                           aaaGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTtgat                                                                                                                                                                                  >  2:979959/1‑114 (MQ=255)
                                                                                                                                                                                   gttcgttACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGt                                             >  1:1134133/1‑239 (MQ=255)
                                                                                                                                                                                                aaCTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCAt                                                                                                                                                                                                                      <  1:632090/57‑1 (MQ=255)
                                                                                                                                                                                                aaCTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCAt                                                                                                                                                                                                                      >  2:632090/1‑57 (MQ=255)
                                                                                                                                                                                                            aaaaaCCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGAc                    <  1:625606/239‑1 (MQ=255)
                                                                                                                                                                                                            aaaaaCCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGAc                    <  2:1134133/239‑1 (MQ=255)
                                                                                                                                                                                                                aCCTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCt                <  2:867716/239‑1 (MQ=255)
                                                                                                                                                                                                                  cTTAAGAATCAATTTACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGc              <  1:601432/239‑1 (MQ=255)
                                                                                                                                                                                                                              tttACCGTAAGCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTCCTga  <  2:904452/239‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                    
CAACTCTATGCCATCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTTTTTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTTTAACAGAGACTTAAAGAAACGTTCGTTACGCTTAACTTTCGGAGAAAAAACCTTAAGAATCAATTTACCGTAATCGGTATCAATAAGCATAACTTTTGTATCATCAATAGAACGAAAAACCTTGATGATATTTATATTATAAGACAAGAAGTCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTCCTGA  >  NC_000913/3800576‑3801036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: