Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 242,498 A→C L269R (CTG→CGG)  fadE ← acyl coenzyme A dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913242,4980AC98.8% 267.7 / NA 81L269R (CTG→CGG) fadEacyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (40/40);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

ACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGATCCCGCT  >  NC_000913/242264‑242724
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                  
aCGAAGACATCTTTCCCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCa                                                                                                                                                                                                                                 <  2:494703/238‑1 (MQ=255)
         tCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACct                                                                                                                                                                                                                              >  1:1082294/1‑232 (MQ=255)
          cgttACCGGGCGTCGTCCCGTTATGGGACGGTACGTCCAGCGGGAGGTGGCGCCGACCAATTTCCCCCCCCGGCGTGGTGGTTGGGATCAGCGCCCAGTTAATGCCTAAATCTTCTCCACCGCCGAGTAGTTTTTCCGGGTCGGAGAGTTTAAACGCCAGCGCAAGCACGGTCGCAAACGGTGCCCGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGccc                                                                                                                                                                                                                      <  2:522981/237‑1 (MQ=255)
           tttACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACctgc                                                                                                                                                                                                                            <  2:732764/232‑1 (MQ=255)
                   gctgcgttcggTTGTGGAACGGTAAGTTCGGCGGGAAGTGGCGAGGACCAATTTCCACGTCCGGCGGGGTGGTTGGGATCAGCGCACAGGTACTGCATAAATCGTCGGCAGCGCCAGATAATTATTCCGGGTAGGAGGTTTTAAACGCCTGCCGGAGCACGGCTGTATGCGGTGCCGGCTTAATGAAGCATTTCTTCCAGGTCCAAGGCATCCCCAGCCCCTGCTGGCCCTGCCATTCg                                                                                                                                                                                                             <  2:556305/229‑1 (MQ=255)
                                     aCGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCg                                                                                                                                                                                           <  2:1082294/239‑1 (MQ=255)
                                       ggTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGgt                                                                                                                                                                                         >  1:1128582/1‑239 (MQ=255)
                                        gTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATccc                                                                                                                                                                                            >  1:171817/1‑235 (MQ=255)
                                        gTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATccc                                                                                                                                                                                            >  1:950204/1‑235 (MQ=255)
                                                             gacgacCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCg                                                                                                                                                                   <  1:18232/239‑1 (MQ=255)
                                                                  acAATGTCCACGCCCGGCGTGGTGGTGGGGATCAACGCGAAGGTAATGCCTAATGCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCGGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAAcc                                                                                                                                                              <  2:797196/238‑1 (MQ=255)
                                                                                 ggCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGt                                                                                                                                               <  1:902336/239‑1 (MQ=255)
                                                                                 ggCGGGGTGGTTGGGATCAGCGCACGGGTACTGCCTAAATCTTCTGCACCGCCGAGTAATGTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGt                                                                                                                                               <  2:610088/239‑1 (MQ=255)
                                                                                      ggtggtTGGGATCAGCGCACAGTTAATGCCAAAATCGTCTGCACCGCCGGGGAATTTTGCCGGTTGGGGGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCTTAATGTCGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGGCAATCCCGGTGTCCGGAATCGCGCCCGCGTCGGAACCCGCTTCCGGGCTGGTCAGTg                                                                                                                                          <  2:258654/239‑1 (MQ=255)
                                                                                       gtggtTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATg                                                                                                                                                                                                       <  1:1063602/177‑1 (MQ=255)
                                                                                       gtggtTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATg                                                                                                                                                                                                       >  2:1063602/1‑177 (MQ=255)
                                                                                       gtggtTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTg                                                                                                                                          <  1:783933/238‑1 (MQ=255)
                                                                                         ggtTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCaa                                                                                                                                       >  1:194151/1‑239 (MQ=255)
                                                                                                 tCAGCGCCCAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAgggg                                                                                                                               <  2:171817/239‑1 (MQ=255)
                                                                                                 tCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGATTAATTTTTCCGGGTCGGAGAGTTTAAACGCCGGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAggg                                                                                                                                <  2:746686/238‑1 (MQ=255)
                                                                                                          aGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGt                                                                                                                                               <  1:126882/214‑1 (MQ=255)
                                                                                                          aGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAggg                                                                                                                                >  1:292547/1‑229 (MQ=255)
                                                                                                          aGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAggg                                                                                                                                <  2:292547/229‑1 (MQ=255)
                                                                                                          aGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCCCCCGCATCGGAACCCGCTTCCGGGCTGGt                                                                                                                                               >  2:126882/1‑214 (MQ=255)
                                                                                                          aGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGAAGACAATCCCGGTGTCCGGAACCGCGCCCGCATCGGACCCGCCTTCCGGGCAGGCCAGTGCCAAGCAGGGGAttc                                                                                                                           >  2:22470/1‑232 (MQ=255)
                                                                                                               aTGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGa                                                                                                                                                                                                   <  2:1051354/157‑1 (MQ=255)
                                                                                                               aTGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGa                                                                                                                                                                                                   >  1:1051354/1‑157 (MQ=255)
                                                                                                               aTGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAggg                                                                                                                                >  1:233136/1‑224 (MQ=255)
                                                                                                               aTGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAggg                                                                                                                                <  2:233136/224‑1 (MQ=255)
                                                                                                               aTGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCAc                                                                                                                  >  1:584152/1‑238 (MQ=255)
                                                                                                               aTGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCAc                                                                                                                  >  1:661364/1‑238 (MQ=255)
                                                                                                                      aaTCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATTCAGACAATCCCCGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAg                                                                                                           >  1:194901/1‑238 (MQ=255)
                                                                                                                      aaTCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGcc                                                                                                                                                                          >  1:1121050/1‑175 (MQ=255)
                                                                                                                      aaTCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGcc                                                                                                                                                                          <  2:1121050/175‑1 (MQ=255)
                                                                                                                      aaTCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCggg                                                                                                                                                    >  1:1164789/1‑197 (MQ=255)
                                                                                                                      aaTCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCggg                                                                                                                                                    <  2:1164789/197‑1 (MQ=255)
                                                                                                                      aaTCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAg                                                                                                           >  1:399625/1‑238 (MQ=255)
                                                                                                                            ctGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCCGACGCGGc                                                                                                    >  1:573957/1‑239 (MQ=255)
                                                                                                                            ctGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGc                                                                                                    >  1:1155339/1‑239 (MQ=255)
                                                                                                                               cACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGGGCCAGCGGAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGAGTCCGGAATCGCGCGCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCAGGCGCCAGACGCGGCGGa                                                                                                 >  2:428979/1‑239 (MQ=255)
                                                                                                                                  cgccgAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGGCCACGCGCCAGACGCGGCAGATAg                                                                                              <  2:950204/239‑1 (MQ=255)
                                                                                                                                  cgccgAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGAcgcg                                                                                                      >  1:357325/1‑231 (MQ=255)
                                                                                                                                  cgccgAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGAcgcg                                                                                                      <  2:357325/231‑1 (MQ=255)
                                                                                                                                   gccgAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGt                                                                                             <  2:194151/239‑1 (MQ=255)
                                                                                                                                    ccgAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGCTAGt                                                                                             >  1:611767/1‑238 (MQ=255)
                                                                                                                                        ttAATTTTTCCGGGGCGGAGATGTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCCCCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCt                                                                                        <  2:573957/238‑1 (MQ=255)
                                                                                                                                                  cGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGt                                                                              <  2:1128582/239‑1 (MQ=255)
                                                                                                                                                      tCGGAAGGTTTAAACGCCAGCCCAAGCCCGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAg                                                                           <  2:399625/238‑1 (MQ=255)
                                                                                                                                                            agTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGt                                                                     <  2:584152/238‑1 (MQ=255)
                                                                                                                                                                      ccAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGAt                                                                                                                             >  1:243083/1‑172 (MQ=255)
                                                                                                                                                                      ccAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGAt                                                                                                                             <  2:243083/172‑1 (MQ=255)
                                                                                                                                                                        aGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCCTCCGGGCTGGTCAGGCCAAAGCAGGGGATCTCCTGACCCCGCCCCAGACGCGGCAGATAGTCATCTTTCTGCCCGTCAGGTCCGTCATGTTGCaaaca                                                        >  2:560812/1‑236 (MQ=255)
                                                                                                                                                                                    gTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCg                                                                                                                                                                                                             <  2:783824/78‑1 (MQ=255)
                                                                                                                                                                                    gTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCg                                                                                                                                                                                                             >  1:783824/1‑78 (MQ=255)
                                                                                                                                                                                             ggTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCAt                                                                                                                                                                                                        >  2:735607/1‑74 (MQ=255)
                                                                                                                                                                                             ggTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCAt                                                                                                                                                                                                        <  1:735607/74‑1 (MQ=255)
                                                                                                                                                                                             ggTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGtt                                   >  1:225983/1‑239 (MQ=255)
                                                                                                                                                                                               tGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGc                                                                                                    >  1:132545/1‑172 (MQ=255)
                                                                                                                                                                                               tGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGc                                                                                                    <  2:132545/172‑1 (MQ=255)
                                                                                                                                                                                               tGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTGGCAACAGTTCGCCCGGGCCTCATGAGTTTg                                 >  1:226182/1‑239 (MQ=255)
                                                                                                                                                                                                        gaTGTACAGTTGGTGCAAGGTCAGACGCCGCCCCCGCCCCTGCTGGCCCTGCCATACGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGGCGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGa                        <  2:870927/238‑1 (MQ=255)
                                                                                                                                                                                                        aaTGTAGCGTTTGTTCCAGGTCGGACGCTTCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGa                        <  1:908353/239‑1 (MQ=255)
                                                                                                                                                                                                         aTGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGccc                                                                                                                                                                                                          >  1:68602/1‑60 (MQ=255)
                                                                                                                                                                                                         aTGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGccc                                                                                                                                                                                                          <  2:68602/60‑1 (MQ=255)
                                                                                                                                                                                                         aTGTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGAc                       >  2:877835/1‑239 (MQ=255)
                                                                                                                                                                                                           gTAGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGCTCTCCTGACCACGCGCCAGACGCGGCAGATAGGGATCTTTCTGTTCGGCAGTGCCGTCATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCCCGCCGACgg                     >  1:482080/1‑239 (MQ=255)
                                                                                                                                                                                                             aGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGc                                                                                                    <  2:499534/158‑1 (MQ=255)
                                                                                                                                                                                                             aGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGc                                                                                                    >  1:499534/1‑158 (MQ=255)
                                                                                                                                                                                                             aGCGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTa                   <  1:877835/239‑1 (MQ=255)
                                                                                                                                                                                                               cGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCg                                                                                                                                                                                                             <  1:87918/51‑1 (MQ=255)
                                                                                                                                                                                                               cGTTTGTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCg                                                                                                                                                                                                             >  2:87918/1‑51 (MQ=255)
                                                                                                                                                                                                                   tGTTCCAGGGAAGACGCATCCCCCGCTCCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGAACACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGcc             <  2:194901/239‑1 (MQ=255)
                                                                                                                                                                                                                    gTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCa            >  2:709308/1‑239 (MQ=255)
                                                                                                                                                                                                                    gTTCCAGGTCAGACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGCGATCTTTCTGCTCGTCAGTGCCGCAATGTTGCAACAGTTCGCCCGGGCCTCATGAGTTTGGCACGCCGACGGTAATCGCCa            >  1:111482/1‑239 (MQ=255)
                                                                                                                                                                                                                               gACGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGATCCCGCt  >  2:79515/1‑238 (MQ=255)
                                                                                                                                                                                                                                aCGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGAt                                                                                          >  1:180798/1‑149 (MQ=255)
                                                                                                                                                                                                                                aCGCATCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGAt                                                                                          <  2:180798/149‑1 (MQ=255)
                                                                                                                                                                                                                                     tCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAAcc                                                                                                                                                              >  1:194019/1‑76 (MQ=255)
                                                                                                                                                                                                                                     tCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAAcc                                                                                                                                                              <  2:194019/76‑1 (MQ=255)
                                                                                                                                                                                                                                     tCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTaa                                                                   >  2:14010/1‑167 (MQ=255)
                                                                                                                                                                                                                                     tCCCCCGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTaa                                                                   <  1:14010/167‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                  
ACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCGGTGTCCGGAATCGCGCCCGCATCGGAACCCGCTTCCGGGCTGGTCAGTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGATCCCGCT  >  NC_000913/242264‑242724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: