Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 743,458 A→C Y211S (TAT→TCT)  ybgI → NIF3 family metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913743,4580AC100.0% 289.8 / NA 77Y211S (TAT→TCT) ybgINIF3 family metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (37/40);  total (37/40)

ACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGAACTGGAACCGCCGGTG  >  NC_000913/743222‑743658
                                                                                                                                                                                                                                            |                                                                                                                                                                                                        
aCTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCtg                                                                                                                                                                                                        <  2:648859/239‑1 (MQ=255)
      cATGCGTGGGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCa                                                                                                                                                                                                   <  2:372657/238‑1 (MQ=255)
      cATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCTAGCAGGGATTGCATTTTTCTGCTGCAg                                                                                                                                                                                                  >  1:736289/1‑239 (MQ=255)
          ccTGGGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAg                                                                                                                                                                                                  <  1:665743/235‑1 (MQ=255)
              tgCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCAt                                                                                                                                                                                          >  1:44524/1‑239 (MQ=255)
                          aGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACgt                                                                                                                                                                              >  1:422039/1‑239 (MQ=255)
                             tGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACgtggt                                                                                                                                                                           <  2:735037/239‑1 (MQ=255)
                               gCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGCTTGCATTTTTCTGCTGCAGGTCACCATCCCACTTAACCtggtgg                                                                                                                                                                         >  2:118053/1‑239 (MQ=255)
                                   ccTGGATTGAAGCGCGGCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTAtt                                                                                                                                                                     <  1:346605/239‑1 (MQ=255)
                                    cTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCa                                                                                                                                                                                              <  2:755356/213‑1 (MQ=255)
                                    cTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCa                                                                                                                                                                                              >  1:755356/1‑213 (MQ=255)
                                         ttGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCa                                                                                                                                                               >  1:968889/1‑239 (MQ=255)
                                         ttGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGAAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCGGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCa                                                                                                                                                               <  2:154552/239‑1 (MQ=255)
                                                  gTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGa                                                                                                                                                       <  2:187652/238‑1 (MQ=255)
                                                            tAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCtgaatg                                                                                                                                            >  1:380674/1‑239 (MQ=255)
                                                                   ttATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGa                                                                                                                                                       <  1:205081/221‑1 (MQ=255)
                                                                    tATGGTGTGGCGATACCGGACCTGGGGTGGTCAGGGGCGTCGCCTGGTGCACGGGCGGGGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATAc                                                                                                                                     <  2:625197/238‑1 (MQ=255)
                                                                             gCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCCGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGGGGGGGTATTCGCGCATTGAGCGGGGTGCtgaat                                                                                                                                             >  2:205081/1‑221 (MQ=255)
                                                                             gCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGCGCGAGGGGCTGAATGAAAATACAGATCTTCAt                                                                                                                           >  1:1073307/1‑239 (MQ=255)
                                                                             gCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGAt                                                                                                                           >  1:427878/1‑239 (MQ=255)
                                                                               gATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCAGGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGAtgt                                                                                                                         <  1:1189140/239‑1 (MQ=255)
                                                                                aTACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGAtgt                                                                                                                         <  2:994126/238‑1 (MQ=255)
                                                                                       aCCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTcg                                                                                                                                                                   >  2:96119/1‑189 (MQ=255)
                                                                                       aCCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTcg                                                                                                                                                                   <  1:96119/189‑1 (MQ=255)
                                                                                           gAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGa                                                                                                             >  2:754666/1‑239 (MQ=255)
                                                                                            aGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGat                                                                                                            >  1:1174603/1‑239 (MQ=255)
                                                                                             ggtggtCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGat                                                                                                            <  1:864341/238‑1 (MQ=255)
                                                                                             ggtggtCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGat                                                                                                            <  2:226662/238‑1 (MQ=255)
                                                                                                 gtCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGGGGCTGAATGGAAATACCGATCTTGATGGGACCTTTATTGATAtt                                                                                                         >  1:1183572/1‑237 (MQ=255)
                                                                                                  tCCAGCGCGTCGCCTGGTGCTCGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTcc                                                                                                       <  1:596704/238‑1 (MQ=255)
                                                                                                   ccAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATAc                                                                                                                                     <  2:1119019/207‑1 (MQ=255)
                                                                                                   ccAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATAc                                                                                                                                     >  1:1119019/1‑207 (MQ=255)
                                                                                                       cgcgTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGACCTTGATGTGACCTTTATTGATATTCCTAAt                                                                                                   >  1:1108108/1‑237 (MQ=255)
                                                                                                        gcgTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTaa                                                                                                    <  2:44524/235‑1 (MQ=255)
                                                                                                        gcgTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATcc                                                                                                 <  2:475679/238‑1 (MQ=255)
                                                                                                           tCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCa                                                                                                                                                                                           <  1:400490/145‑1 (MQ=255)
                                                                                                           tCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCa                                                                                                                                                                                           >  2:400490/1‑145 (MQ=255)
                                                                                                           tCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCa                                                                                             >  1:1162946/1‑239 (MQ=255)
                                                                                                            cGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCtg                                                                                                                                                <  1:921279/187‑1 (MQ=255)
                                                                                                            cGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCtg                                                                                                                                                >  2:921279/1‑187 (MQ=255)
                                                                                                                tGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACg                                                                                        <  1:754666/239‑1 (MQ=255)
                                                                                                                             gcggGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGat                                                                                                            <  2:1059798/206‑1 (MQ=255)
                                                                                                                             gcggGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGat                                                                                                            >  1:1059798/1‑206 (MQ=255)
                                                                                                                                   aatgTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACGGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGAGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTAATGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGaa                                                                     <  1:583094/236‑1 (MQ=255)
                                                                                                                                   aaggTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGa                                                                      <  1:53854/235‑1 (MQ=255)
                                                                                                                                          tATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATAc                                                                                                                                     <  2:56817/168‑1 (MQ=255)
                                                                                                                                          tATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATAc                                                                                                                                     >  1:56817/1‑168 (MQ=255)
                                                                                                                                                 aaccccgcGCGTTGTGGCGTGGATGCTTTTATTACTGGCTAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAgcg                                                        <  1:1074637/234‑1 (MQ=255)
                                                                                                                                                 aGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTcgcg                                                                                                                                                                 >  1:256180/1‑133 (MQ=255)
                                                                                                                                                 aGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTcgcg                                                                                                                                                                 <  2:256180/133‑1 (MQ=255)
                                                                                                                                                 aGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAgcgc                                                       >  1:1015362/1‑239 (MQ=255)
                                                                                                                                                   cgccgcGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGat                                                                                                            <  2:730716/184‑1 (MQ=255)
                                                                                                                                                   cgccgcGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGat                                                                                                            >  1:730716/1‑184 (MQ=255)
                                                                                                                                                    gccgcGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGcgcg                                                      <  2:1162946/237‑1 (MQ=255)
                                                                                                                                                         gcgTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCgtt                                                    >  1:539956/1‑234 (MQ=255)
                                                                                                                                                         gcgTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCgtt                                                    <  2:539956/234‑1 (MQ=255)
                                                                                                                                                                                     ggCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGt                                                                                                                                                                                                <  2:38236/66‑1 (MQ=255)
                                                                                                                                                                                     ggCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGt                                                                                                                                                                                                >  1:38236/1‑66 (MQ=255)
                                                                                                                                                                                     ggCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCCCTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGGAGTGcagg                   >  1:1183069/1‑239 (MQ=255)
                                                                                                                                                                                     ggCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCa                                                                                >  1:1123461/1‑178 (MQ=255)
                                                                                                                                                                                     ggCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCa                                                                                <  2:1123461/178‑1 (MQ=255)
                                                                                                                                                                                                      gACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGAtgt                                                                                                                         <  2:853143/120‑1 (MQ=255)
                                                                                                                                                                                                      gACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGAtgt                                                                                                                         >  1:853143/1‑120 (MQ=255)
                                                                                                                                                                                                       aCCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCtg                                                                                                                                                                                                        >  2:572323/1‑40 (MQ=255)
                                                                                                                                                                                                       aCCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGTTGAAACGGCGGTCGTGCCGGAACTGGAACCCCCgggg  >  1:230322/1‑236 (MQ=255)
                                                                                                                                                                                                            tcattcaGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATAc                                                                                                                                     >  1:565588/1‑102 (MQ=255)
                                                                                                                                                                                                            tcattcaGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATAc                                                                                                                                     <  2:565588/102‑1 (MQ=255)
                                                                                                                                                                                                                 caGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGat                                                                                                            <  1:325312/122‑1 (MQ=255)
                                                                                                                                                                                                                 caGCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGat                                                                                                            >  2:325312/1‑122 (MQ=255)
                                                                                                                                                                                                                   gCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCAt                                                                                            >  2:656421/1‑136 (MQ=255)
                                                                                                                                                                                                                   gCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCAt                                                                                            <  1:656421/136‑1 (MQ=255)
                                                                                                                                                                                                                   gCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAggg                                                                           >  1:120446/1‑153 (MQ=255)
                                                                                                                                                                                                                   gCCCGCGAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAggg                                                                           <  2:120446/153‑1 (MQ=255)
                                                                                                                                                                                                                      cgcgAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGat                                                                                                            >  2:774415/1‑117 (MQ=255)
                                                                                                                                                                                                                      cgcgAGCAGGGATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGat                                                                                                            <  1:774415/117‑1 (MQ=255)
                                                                                                                                                                                                                              gggATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATAc                                                                                                                                     >  1:1164378/1‑84 (MQ=255)
                                                                                                                                                                                                                              gggATTGCATTTTTCTGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATAc                                                                                                                                     <  2:1164378/84‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                        
ACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGAACTGGAACCGCCGGTG  >  NC_000913/743222‑743658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: