Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,402,453	T→G	Q268P (CAG→CCG)	abgB ←	p‑aminobenzoyl‑glutamate hydrolase, B subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,402,453	0	T	G	100.0%	179.9 / NA	56	Q268P (CAG→CCG)	abgB	p‑aminobenzoyl‑glutamate hydrolase, B subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/28);  total (28/28)

GACTGTTTTGCCGATCGTTGGAGGTGAGCGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGACCACGTTGGGCGAGATCCCGCCGCTATTTGTGATGGCATAGTGTACGCGCGCTTTTTCAATAATATGTTCGTTGAGGAAGTTGGTGCCA‑‑GTGGTCATCAACGTTACGGCATCAAGGGCGCTGCGTCCCAAATGAGGGGAATTCGCGGCATGTGCTGCGATCCCTTTAAAGCGCCATGA  >  NC_000913/1402220‑1402688                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               gACTGTTTTGCCGATCGTTGGAGGTGAGCGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGTTCATAAATATGCGGCACg                                                                                                                                                                                                                                           >  2:391163/1‑239 (MQ=255) gACTGTTTTGCCGATCGTTGGAGGTGAGCGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACg                                                                                                                                                                                                                                           >  1:567934/1‑239 (MQ=255)  aCTGTTTTGCCGATCGTTGGAGGTGAGCGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACg                                                                                                                                                                                                                                           >  1:951137/1‑238 (MQ=255)    tGTTTTGCCGATCGTTGGAGGTGAGCGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCacgacg                                                                                                                                                                                                                                        >  1:419488/1‑239 (MQ=255)       tttGCCGATCGTTGGAGGTGAGCGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTc                                                                                                                                                                                                                                     >  1:485572/1‑239 (MQ=255)             gATCGTTGGAGTGTAGCGTAGCCTGATTTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAAGGGGATAGGGCCTGGTACATGGCATTTCCTAAGGTGCGACTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCCCCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCg                                                                                                                                                                                                                               <  2:915375/239‑1 (MQ=255)                cGTTGGAGGTGAGCGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGCCATTTCgggg                                                                                                                                                                                                                            >  1:1189933/1‑239 (MQ=255)                   tGGAGGTGAGCGTAGCGTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCTGTCATCAATGCCGCGCCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGgc                                                                                                                                                                                                                          <  2:170510/238‑1 (MQ=255)                   tGGAGGTGAGCGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGgc                                                                                                                                                                                                                          <  1:701183/238‑1 (MQ=255)                        gTGAGCGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGgat                                                                                                                                                                                                                    >  1:788288/1‑239 (MQ=255)                           aGCGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGgataag                                                                                                                                                                                                                 >  1:675118/1‑239 (MQ=255)                             cGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGgataagat                                                                                                                                                                                                               >  1:294770/1‑239 (MQ=255)                             cGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGgataagat                                                                                                                                                                                                               >  1:143811/1‑239 (MQ=255)                             cGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGga                                                                                                                                                                                                                     <  1:423929/233‑1 (MQ=255)                             cGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGga                                                                                                                                                                                                                     >  2:423929/1‑233 (MQ=255)                                aGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATaaa                                                                                                                                                                                                            <  2:61770/239‑1 (MQ=255)                                   cTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAg                                                                                                                                                                                                           <  1:134665/237‑1 (MQ=255)                                    tGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑c                                                                                                                                                                                                       <  1:391163/239‑1 (MQ=255)                                     gAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑Ct                                                                                                                                                                                                      >  1:980606/1‑239 (MQ=255)                                     gAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑Ct                                                                                                                                                                                                      >  1:949602/1‑239 (MQ=255)                                     gAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTGTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑Ct                                                                                                                                                                                                      <  2:771569/239‑1 (MQ=255)                                                            tcttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGAc                                                                                                                                                                                     <  2:567934/233‑1 (MQ=255)                                                                   aGTGCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGACCACGTTGGGCga                                                                                                                                                                         <  2:411396/238‑1 (MQ=255)                                                                     ttCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCTGGGGCGCGGTTAAGATAAAGCA‑CTTCTGCCTGCGCCTGGACCACGTTGGGCgcga                                                                                                                                                                       >  1:548970/1‑238 (MQ=255)                                                                              gggtaacaaaaaGGGGATGGGGCCTGGTACATGGCATTTTCTAGGGGGCGTTCGGGGAGATAACTGGAACGGGTTTTGTCGAGGCGGCATTCAACCTGGGTTTCGATCATCAAGGCCGCCCCTTGGGGGTTTTGGGCGCCCCGATCATAAATATGCGGCCCGTGGTTCATTTCGGGGGGGGGGATAAGAAAAAGCC‑TTTTTGCCTGCGCCTGGACCCGGTGGGGCGAGATCCCGCCGCt                                                                                                                                                             <  2:1189933/231‑1 (MQ=255)                                                                                          tgggtgggggCTTGGTACATGGATTTTTATAAGGGTCGATCGGGAAGATATAGGAAACAGGTTTTTTCAGACCGGCATTAAACCGTGTTTTGGGTCATAAAGCCCGCACTTTGGTAGATTGGGGCGACCCAATAAAAAATAGGCGGCCCTCGGGTAATTTGGGGGGCGGGGATAAGATAAAGCC‑CTTCTCCCGGCGCCTGGCCCCCGTTGGGCGAAATCCCGCCGCTATTTGTGATGGc                                                                                                                                                 <  2:675118/232‑1 (MQ=255)                                                                                             gATAGGGCCTGGTAAGTGGCATTTGCTAGGGTGCGATCGGGGAGATAACTGGAACAGGCTTGGTGGAAGCGGCTTTAAACCGGGGTTTCGGTCATCAATCCCGCCCCTTCGGCGATTTTGGCGCCCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGAAAAGAAAAAGCC‑CTTCTGCCGGCGCCTGGACCCCGTGGGGCGAGATCccgccg                                                                                                                                                               <  2:253870/222‑1 (MQ=255)                                                                                                       ggTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGgc                                                                                                                                                                                                                          <  1:742132/154‑1 (MQ=255)                                                                                                       ggTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGgc                                                                                                                                                                                                                          >  2:742132/1‑154 (MQ=255)                                                                                                       ggTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGACCACGTTGGGCGAGATCCCGCCGCTATTTGTGa                                                                                                                                                     >  1:253870/1‑222 (MQ=255)                                                                                                             tGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGACCACGTTGGGCGAGATCCCGCCGCTATTTGTGATGGCATAGTGTACGCGCGCtttt                                                                                                                              >  1:934469/1‑239 (MQ=255)                                                                                                              ggtaTTTTCTAGGGTGCTATTGGTGATATACGTGTAACAGGTTTTGTCAGCGCGGCATTACCCCTTGGTTTCGGTCAACAAGGCCGCCCCTTCGGCGATTTGGGCGCCCCAAAAAAAAATATGCGCCGCGTCGGTCTTTTCGGGGGCGCGGATAAGATAAAGCT‑TTTCTGCCGGCGCCTGGACCCCGTGGGGTAAGTCCCCGCCGCTATTTGTGATGGCATAGTGTACGCGCGCttttt                                                                                                                             <  2:157363/236‑1 (MQ=255)                                                                                                                 tttttCTAAGGTTCGATTGGGGAGATAACGGGAACAGGCTTTGTCGAGGCGGCATTCACCCGTGGTTTCGGTCATCAATGCCGCCCCTTCGGCTGTTTGGGCGACCCGATAAAAAAAAGGCGGCACGTCGGTCATTTCGGGGGGGCGGATAAGAAAAAGCC‑CTTCTGCCTGCGCCTGGACCACGTGGGGCGAGATCCCGCCGCTATTTGTGATGGCATAGTGTACGCGCGCTTTTTCa                                                                                                                           <  2:788288/237‑1 (MQ=255)                                                                                                                  ttttCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGAc                                                                                                                                                                                     >  1:983969/1‑179 (MQ=255)                                                                                                                  ttttCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGAc                                                                                                                                                                                     <  2:983969/179‑1 (MQ=255)                                                                                                                    ttagagggTGCGATTCGGGAGAAAACTGAAACAGGTTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATAAAGGCCGCCCTTGGGGGGATTTTGGCTCCCCGAGAAAAAATAGGGGGAGCGACGGTCTTGTCGGGGGCGCGGATAAGAAAAACCA‑CTGCTGCCGGCGCCTGGACCACTTTGGGCGAGATCCCGCCGCTATTtg                                                                                                                                                        <  2:1099216/200‑1 (MQ=255)                                                                                                                              cGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGgata                                                                                                                                                                                                                   <  2:306958/138‑1 (MQ=255)                                                                                                                              cGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGgata                                                                                                                                                                                                                   >  1:306958/1‑138 (MQ=255)                                                                                                                              cGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGga                                                                                                                                                                                                                     >  2:1061437/1‑136 (MQ=255)                                                                                                                              cGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGga                                                                                                                                                                                                                     <  1:1061437/136‑1 (MQ=255)                                                                                                                              cGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGACCACGTTGGGCGAGATCCCGCCGCTATTTGTGATGGCATa                                                                                                                                              >  1:1099216/1‑206 (MQ=255)                                                                                                                              cGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGACCACGTTGGGCGAGATCCCGCCGCTATTTGTGATGGCATAGTGTACGCGCGCTTTTTCAATAATATGTTCGtt                                                                                                             >  2:1193107/1‑239 (MQ=255)                                                                                                                                           aaCTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGgata                                                                                                                                                                                                                   >  2:257932/1‑125 (MQ=255)                                                                                                                                           aaCTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGgata                                                                                                                                                                                                                   <  1:257932/125‑1 (MQ=255)                                                                                                                                            tcggaaaagggCTTGGTCGAGGCGACTTTAAACGGGGTTTTGGGCCATCAATGCAGACCGTTCGGAGATTTGGGCACCCCGTTAATATTTATGCGCCACGCGGGTATTTGCGGGGGCGC‑GATTAAAAAAATCATCTTCGGCGTCCGCTGGGTCTAAGTGGGAGGGGTTCCCGCCGCTATTTGTGATGGCATTGTGTACGCGGGCTTTTCCATTAATATGTTCGTTGAGGAAGTTGGTgg                                                                                               <  2:951402/230‑2 (MQ=255)                                                                                                                                             cTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTctgcc                                                                                                                                                                                                <  2:915621/141‑1 (MQ=255)                                                                                                                                             cTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTctgcc                                                                                                                                                                                                >  1:915621/1‑141 (MQ=255)                                                                                                                                                                      aTTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCCACCCGATCATAAATATGAGGCACGTCGGCCATTTCGGGGGCGTGGATAAGATATAGCC‑CTTTTGCCTGCGCCTGGACCACGTTGGGCGAGATCCCGCCGTTATTTGTGATGGCATAGTGTACGCGCGCTTTTTCAATAATATGTTCGTTGAGGAAGTTGGTGCCA‑‑GTGGTCATCAACGTTACGGCATc                                                                    >  2:1085223/1‑238 (MQ=255)                                                                                                                                                                                gttttCGGTAATAGATGCCGCACCTTCGGCGTTTTTGGCGCACCGATCATAAATAGGCGGCGCGTGGGTCTTTTGGGGGGCGGGAATAAGATAAAGCC‑TGTCTGCCTGCGCCTGGACCACGTTGGGCGAGATCCCGCCGCTATTTGTGATGGCATAGTGTACGCGCGCTTTTTCAATAATATGTTCGTTGAGGAAGTTGGTGCCA‑‑GTGGTCATCAACGTTACGGCATCAAGGGCGCTg                                                          <  2:951137/236‑1 (MQ=255)                                                                                                                                                                                      ggTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGACCACGTTGGGCGAGCTCCCGCCGCTATTTGTGATGGCATAGTGTACGCGCGCTTTTTCAATAATCTGTTCGTTGAGGAAGTTGGTGCCA‑‑GTGGTCATCAACGTTACGGCATCAATGGCGCTGCGTCCCa                                                   >  2:806729/1‑239 (MQ=255)                                                                                                                                                                                         catcaATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGACCACGTTGGGCGAGATCCCGCCGCTATTTGTGATGGCATAGTGTACGCGCGCTTTTTCAATAATATGTTCGTTGAGGAAGTTGGTGCCA‑‑GTGGTCATCAACGTTACGGCATCAAGGGCGCTGCGTCCCCAAt                                                >  1:579951/1‑239 (MQ=255)                                                                                                                                                                                                         tCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGgc                                                                                                                                                                                                                          >  1:785666/1‑56 (MQ=255)                                                                                                                                                                                                         tCGGCGATTTTGGCGACCCGATCATAAATATGCGGCACGTCGGTCATTTCGGGGgc                                                                                                                                                                                                                          <  2:785666/56‑1 (MQ=255)                                                                                                                                                                                                         tCGGCGATTTGGGCGACCCGATCATAAATATGCGGCCCGTCGGTCATTGCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGCCCACGTTGGGCGAGATCCCGCCGCTATTTGTGATGGCATAGTGTACGCGCGCTTTTTCAATAATATGTTCGTTGAGGAAGTTGGTGCCA‑‑GTGGTCATCAACGTTACGGCATCAAGGGCGCTGCGTCCCAAATGAGGGGAATTCGCGGc                                <  2:416604/239‑1 (MQ=255)                                                                                                                                                                                                                             tacATAAATATGCGGCACGTCGTTCATTACGGGGGCGCGAATAATATAAGGCA‑CTTCGGCCGGCGCCTGGCCCCGGTGGGGGGAGATCCCGCCGCTATTTGTGATGGCATAGTGTACGCGCGCTTTTTCAATAATATGTTCGTTGAGGAAGTTGGTGCCA‑‑GTGGTCATCAACGTTACGGCATCAAGGGCGCTGCGTCCCAAATGAGGGGAATTCGCGGAATGTGCTGCGATCCCTTTaa            <  2:124461/237‑1 (MQ=255)                                                                                                                                                                                                                              tCATAAATATGCGGCGCGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCG‑CTTCGGCCGGGGCCTGGACCGCGTTGGGAGAGATCCCGCCGCTATTTGTGa                                                                                                                                                     <  1:158672/103‑1 (MQ=255)                                                                                                                                                                                                                                       tgcggcCCGTCGGTCCTTTGGGGGGCGGGGAAAAGATAAAGCT‑CTTCTGCCGGCGCCTGGACCGCGTGGGGCGAGATCCCGCCGCTATTTGTGATGGCATAGTGTACGCGCGCTTTTTCAATAATATGTTCGTTGAGGAAGTTGGTGCCAGTGTGGTCATCAACGTTACGGCATCAAGGGCGCTGCGTCCCAAATGAGGGGAATTCGCGGc                                <  2:674036/211‑1 (MQ=255)                                                                                                                                                                                                                                       tgcggcACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGACCACGTTGGGCGAGATCCCGCCGCTATTTGTGATGGCATAGTGTACGCGCGCTTTTTCAATAATATGTTCGTTGAGGAAGTTGGTGCCA‑‑GTGGTCATCAACGTTACGGCATCAAGGGCGCTGCGTCCCAAATGAGGGGAATTCGCGGCATGTGCTGCGATCCCTTTAAAGCGCCatga  >  2:1148827/1‑239 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               GACTGTTTTGCCGATCGTTGGAGGTGAGCGTAGCCTGAATTTGTTTCGCAAAAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTGCACGTCGGTCATTTCGGGGGCGCGGATAAGATAAAGCA‑CTTCTGCCTGCGCCTGGACCACGTTGGGCGAGATCCCGCCGCTATTTGTGATGGCATAGTGTACGCGCGCTTTTTCAATAATATGTTCGTTGAGGAAGTTGGTGCCA‑‑GTGGTCATCAACGTTACGGCATCAAGGGCGCTGCGTCCCAAATGAGGGGAATTCGCGGCATGTGCTGCGATCCCTTTAAAGCGCCATGA  >  NC_000913/1402220‑1402688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑