Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,049,948	T→G	S1671A (TCT→GCT)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,049,948	0	T	G	100.0%	195.9 / NA	52	S1671A (TCT→GCT)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (32/20);  total (32/20)

CGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCT  >  NC_000913/2049712‑2050175                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                    cGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACcgct                                                                                                                                                                                                                                   <  1:1058097/239‑1 (MQ=255)                    gACATTCACGCTACCGGAAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATtc                                                                                                                                                                                                                       <  1:839067/232‑1 (MQ=255)                                 ccGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCCGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAggg                                                                                                                                                                                                   >  2:1163616/1‑239 (MQ=255)                                                   aGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGGGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACCGCaa                                                                                                                                                                                  >  1:1190207/1‑238 (MQ=255)                                                         aCTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACt                                                                                                                                                                                                                            <  2:461750/190‑1 (MQ=255)                                                         aCTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACt                                                                                                                                                                                                                            >  1:461750/1‑190 (MQ=255)                                                                                           tCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaaaa                                                                                                                                         <  1:323043/239‑1 (MQ=255)                                                                                                           aGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGa                                                                                                                          <  2:236783/238‑1 (MQ=255)                                                                                                                    gAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAAcc                                                                                                                       >  1:354621/1‑232 (MQ=255)                                                                                                                     aGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCAt                                                                                                                                                                                          <  2:173723/164‑1 (MQ=255)                                                                                                                     aGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCAt                                                                                                                                                                                          >  1:173723/1‑164 (MQ=255)                                                                                                                     aGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCa                                                                                                                >  1:296409/1‑238 (MQ=255)                                                                                                                     aGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCAc                                                                                                               >  2:626640/1‑239 (MQ=255)                                                                                                                     aGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCAc                                                                                                               >  1:1189576/1‑239 (MQ=255)                                                                                                                     aGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCAc                                                                                                               >  1:18445/1‑239 (MQ=255)                                                                                                                     aGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACATCCGTCAc                                                                                                               >  2:909596/1‑239 (MQ=255)                                                                                                                       cGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGGAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGc                                                                                                             >  1:971051/1‑239 (MQ=255)                                                                                                                                    ggCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCa                                                                                                                                                                            >  2:244196/1‑163 (MQ=255)                                                                                                                                    ggCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCa                                                                                                                                                                            <  1:244196/163‑1 (MQ=255)                                                                                                                                    ggCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGt                                                                                                                  >  1:271337/1‑221 (MQ=255)                                                                                                                                    ggCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGt                                                                                                                  <  2:271337/221‑1 (MQ=255)                                                                                                                                    ggCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCa                                                                                                 >  1:472740/1‑238 (MQ=255)                                                                                                                                       aCCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCg                                                                                              <  1:626640/238‑1 (MQ=255)                                                                                                                                                        tAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCATTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGgat                                                                                   <  1:2691/232‑1 (MQ=255)                                                                                                                                                               aaCAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATtcgtc                                                                                                                                                                                                                    >  1:493075/1‑96 (MQ=255)                                                                                                                                                               aaCAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATtcgtc                                                                                                                                                                                                                    <  2:493075/96‑1 (MQ=255)                                                                                                                                                               aaCAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaa                                                                                                                                           <  2:612880/169‑1 (MQ=255)                                                                                                                                                               aaCAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaa                                                                                                                                           >  1:612880/1‑169 (MQ=255)                                                                                                                                                               aaCAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCgg                                                                                                                           >  2:1042170/1‑185 (MQ=255)                                                                                                                                                               aaCAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCgg                                                                                                                           <  1:1042170/185‑1 (MQ=255)                                                                                                                                                               aaCAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCAc                                                                                                   <  2:757013/209‑1 (MQ=255)                                                                                                                                                               aaCAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCAc                                                                                                   >  1:757013/1‑209 (MQ=255)                                                                                                                                                               aaCAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTc                                                                       >  2:208335/1‑237 (MQ=255)                                                                                                                                                               aaCAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTc                                                                       >  1:1131885/1‑237 (MQ=255)                                                                                                                                                                         gtgtCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTg                                                                                                                                                                                                                                  <  1:5353/72‑1 (MQ=255)                                                                                                                                                                         gtgtCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTg                                                                                                                                                                                                                                  >  2:5353/1‑72 (MQ=255)                                                                                                                                                                         gtgtCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAag                                                                                                         <  1:480274/193‑1 (MQ=255)                                                                                                                                                                         gtgtCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAag                                                                                                         >  2:480274/1‑193 (MQ=255)                                                                                                                                                                         gtgtCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCtt                                                           >  1:1105855/1‑239 (MQ=255)                                                                                                                                                                           gtCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTtgt                                                         >  1:46604/1‑239 (MQ=255)                                                                                                                                                                              aGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGAc                                                      >  2:868714/1‑239 (MQ=255)                                                                                                                                                                                 gATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGa                                                       <  2:18445/235‑1 (MQ=255)                                                                                                                                                                                    aCGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAag                                                                                                         <  1:906522/182‑1 (MQ=255)                                                                                                                                                                                    aCGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAag                                                                                                         >  2:906522/1‑182 (MQ=255)                                                                                                                                                                                      gAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCg                                              >  1:110514/1‑239 (MQ=255)                                                                                                                                                                                             gTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGATACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCgg                                       >  2:818384/1‑239 (MQ=255)                                                                                                                                                                                                  tttGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGa                                  >  2:202250/1‑239 (MQ=255)                                                                                                                                                                                                    tGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCAcc                                               <  2:1095684/224‑1 (MQ=255)                                                                                                                                                                                                    tGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCAcc                                               >  1:1095684/1‑224 (MQ=255)                                                                                                                                                                                                               gCTGGTACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGc                     <  2:46604/239‑1 (MQ=255)                                                                                                                                                                                                                    tACCGCAAAACTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAg                      >  1:563989/1‑233 (MQ=255)                                                                                                                                                                                                                           aaaCTAGCCTCCTTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGa         <  2:110514/239‑1 (MQ=255)                                                                                                                                                                                                                                   ctcctTAACCGCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCt  >  2:930498/1‑238 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                    CGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCT  >  NC_000913/2049712‑2050175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑