Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,226,626 A→C H215Q (CAT→CAG yohF ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,226,6260AC100.0% 312.1 / NA 84H215Q (CAT→CAGyohFputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (45/39);  total (45/39)

GATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCGCTTTGGTTAACCCACCGAGCGCATGTTTAGCGGCTGTGTAGGCGCTGGCATCCGGCAGCGGCGTATGTTCATGTACCGACGTAA  >  NC_000913/2226393‑2226858
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                        
gATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGCGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGGTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGt                                                                                                                                                                                                                                     >  2:926334/1‑239 (MQ=255)
     ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTgcg                                                                                                                                                                                                                                 >  2:1145808/1‑238 (MQ=255)
     ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTgcg                                                                                                                                                                                                                                 >  2:254828/1‑238 (MQ=255)
          tCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGTGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCaaa                                                                                                                                                                                                                            >  2:1157407/1‑238 (MQ=255)
          tCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCCGGCTGGCAATCTCCTGCGTTGCGCCaaa                                                                                                                                                                                                                            >  2:1124593/1‑238 (MQ=255)
          tCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCaaa                                                                                                                                                                                                                            >  1:298863/1‑238 (MQ=255)
                 gcATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCa                                                                                                                                                                                                                    <  1:254828/239‑1 (MQ=255)
                          aTCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCg                                                                                                                                                                                                           >  1:202552/1‑239 (MQ=255)
                             cGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAg                                                                                                                                                                                                        >  2:786777/1‑239 (MQ=255)
                                          cTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCgg                                                                                                                                                                                            >  1:605274/1‑238 (MQ=255)
                                                   cgCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCt                                                                                                                                                                                                     <  2:277233/220‑1 (MQ=255)
                                                   cgCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCt                                                                                                                                                                                                     >  1:277233/1‑220 (MQ=255)
                                                   cgCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGt                                                                                                                                                                                   <  1:786777/238‑1 (MQ=255)
                                                      ttGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCt                                                                                                                                                                               <  2:949826/239‑1 (MQ=255)
                                                       tgtgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCaaaa                                                                                                                                                                                                                           <  1:56245/194‑1 (MQ=255)
                                                       tgtgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCaaaa                                                                                                                                                                                                                           >  2:56245/1‑194 (MQ=255)
                                                           cGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCa                                                                                                                                                                           >  2:1019017/1‑238 (MQ=255)
                                                             tCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATcc                                                                                                                                                                        >  1:547993/1‑239 (MQ=255)
                                                                        cGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCcattca                                                                                                                                                              <  2:298863/238‑1 (MQ=255)
                                                                               aaTATTCCCCAATGAAATATCCACGCCCCTCCCTCCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCGCTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCt                                                                                                                                                                               <  2:865734/214‑1 (MQ=255)
                                                                                    tACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGAt                                                                                                                                                 <  1:29397/239‑1 (MQ=255)
                                                                                      cccAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATc                                                                                                                                                >  2:72697/1‑238 (MQ=255)
                                                                                         aaTGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATg                                                                                                                                                                     >  1:865734/1‑214 (MQ=255)
                                                                                         aaTGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGcc                                                                                                                                             >  1:1008094/1‑238 (MQ=255)
                                                                                         aaTGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGcc                                                                                                                                             >  2:439060/1‑238 (MQ=255)
                                                                                         aaTGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGCCATCCATGCCATTCATTGGCGGGGCGATCGcc                                                                                                                                             >  1:1062540/1‑238 (MQ=255)
                                                                                          aTGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAACCGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGcccc                                                                                                                                           <  1:1124593/239‑1 (MQ=255)
                                                                                           tGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACg                                                                                                                                                                                    >  2:289031/1‑197 (MQ=255)
                                                                                           tGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACg                                                                                                                                                                                    <  1:289031/197‑1 (MQ=255)
                                                                                           tGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCa                                                                                                                                          >  2:1091753/1‑239 (MQ=255)
                                                                                                         aCCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGtt                                                                                                                            <  1:439060/239‑1 (MQ=255)
                                                                                                           cTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGTGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCa                                                                                                                          <  1:1157407/239‑1 (MQ=255)
                                                                                                                     tATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCg                                                                                                                                                                                                                                      >  1:963930/1‑121 (MQ=255)
                                                                                                                     tATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCg                                                                                                                                                                                                                                      <  2:963930/121‑1 (MQ=255)
                                                                                                                     tATTCTGGATTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAACCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCGCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCt                                                                                                                 <  1:926334/238‑1 (MQ=255)
                                                                                                                       ttCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAgg                                                                                                                                        >  1:1176666/1‑213 (MQ=255)
                                                                                                                       ttCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAgg                                                                                                                                        <  2:1176666/213‑1 (MQ=255)
                                                                                                                          tGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGcc                                                                                                           <  1:72697/239‑1 (MQ=255)
                                                                                                                            ggTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCt                                                                                                                                                                                                     <  1:793170/147‑1 (MQ=255)
                                                                                                                            ggTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCt                                                                                                                                                                                                     >  2:793170/1‑147 (MQ=255)
                                                                                                                            ggTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCg                                                                                                                              >  1:709091/1‑218 (MQ=255)
                                                                                                                            ggTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCg                                                                                                                              <  2:709091/218‑1 (MQ=255)
                                                                                                                            ggTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATg                                                                                                             >  1:78456/1‑235 (MQ=255)
                                                                                                                            ggTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATg                                                                                                             >  1:151263/1‑235 (MQ=255)
                                                                                                                               tGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGAcc                                                                                                      <  2:1151847/239‑1 (MQ=255)
                                                                                                                                 aaCTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGcc                                                                                                           >  1:626576/1‑232 (MQ=255)
                                                                                                                                 aaCTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGcc                                                                                                           <  2:626576/232‑1 (MQ=255)
                                                                                                                                 aaCTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCa                                                                                                     >  1:592890/1‑238 (MQ=255)
                                                                                                                                 aaCTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCa                                                                                                     >  1:466399/1‑238 (MQ=255)
                                                                                                                                      tgGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGcccc                                                                                                                                           <  1:951365/195‑1 (MQ=255)
                                                                                                                                      tgGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGcccc                                                                                                                                           >  2:951365/1‑195 (MQ=255)
                                                                                                                                            cGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCa                                                                                         <  2:1008094/239‑1 (MQ=255)
                                                                                                                                            cGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCa                                                                                         <  2:466399/239‑1 (MQ=255)
                                                                                                                                               cAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCg                                                                                      <  1:5586/239‑1 (MQ=255)
                                                                                                                                                     aaaGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCGCTTTgg                                                                                <  1:338917/239‑1 (MQ=255)
                                                                                                                                                       aGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCGCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCGCTTTGGtt                                                                              <  1:1027289/239‑1 (MQ=255)
                                                                                                                                                            ccATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATg                                                                                                                                                                     >  1:976848/1‑147 (MQ=255)
                                                                                                                                                            ccATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATg                                                                                                                                                                     <  2:976848/147‑1 (MQ=255)
                                                                                                                                                            ccATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGc                                                                                                            >  2:378728/1‑204 (MQ=255)
                                                                                                                                                            ccATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGc                                                                                                            <  1:378728/204‑1 (MQ=255)
                                                                                                                                                            ccATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCGCTTTGGTTAAccc                                                                         >  1:537149/1‑239 (MQ=255)
                                                                                                                                                                         aCGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCCCCATCGCCATCGCTTTGGTTAACCCACCCACCGCATGt                                                            >  2:48723/1‑239 (MQ=255)
                                                                                                                                                                              tGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCaa                                                                                                                      >  1:348786/1‑176 (MQ=255)
                                                                                                                                                                              tGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCaa                                                                                                                      <  2:348786/176‑1 (MQ=255)
                                                                                                                                                                                cccGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCaa                                                                                                                      <  2:42871/174‑1 (MQ=255)
                                                                                                                                                                                cccGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCaa                                                                                                                      >  1:42871/1‑174 (MQ=255)
                                                                                                                                                                                cccGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAg                                                                                              <  2:423217/198‑1 (MQ=255)
                                                                                                                                                                                cccGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAg                                                                                              >  1:423217/1‑198 (MQ=255)
                                                                                                                                                                                               tGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCtt                                                                                                                                                                                        <  2:918066/93‑1 (MQ=255)
                                                                                                                                                                                               tGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCtt                                                                                                                                                                                        >  1:918066/1‑93 (MQ=255)
                                                                                                                                                                                               tGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCGCTTTGGTTAACCCACCGAGCGCATGTTTAGCGGCTGTGTAGgc                                           >  2:230905/1‑234 (MQ=255)
                                                                                                                                                                                                   cccTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATtggcgtg                                                                                                                                                      <  1:694882/123‑1 (MQ=255)
                                                                                                                                                                                                   cccTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATtggcgtg                                                                                                                                                      >  2:694882/1‑123 (MQ=255)
                                                                                                                                                                                                      tCCGAACAAAGCCACACCCCCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCGCTTTGGtt                                                                              <  2:743019/192‑1 (MQ=255)
                                                                                                                                                                                                      tCCGAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCGCTTTGGtt                                                                              >  1:743019/1‑192 (MQ=255)
                                                                                                                                                                                                         gAACAAAGCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCGCTTTGGTTAACCCACCGAGCGCATGTTTAGCGGCTGTGTAGGCGCTGGCATCCGGCAg                            <  2:537149/239‑1 (MQ=255)
                                                                                                                                                                                                                gCCACACCACCAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCGCTTTGGTTAACCCACCGAGCGCATGTTTAGCGGCTGTGTAGGCGCTGGCATCCGGCAGCGGCGTa                     >  1:22584/1‑239 (MQ=255)
                                                                                                                                                                                                                     acctccAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGTCTCCGCGTCGGGCTTCACGTCGCt                                                                                                                                                                               <  2:30531/80‑1 (MQ=255)
                                                                                                                                                                                                                     accaccAGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCt                                                                                                                                                                               >  1:30531/1‑80 (MQ=255)
                                                                                                                                                                                                                          caGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCt                                                                                                                                                                               >  1:519424/1‑75 (MQ=255)
                                                                                                                                                                                                                          caGGCTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCt                                                                                                                                                                               <  2:519424/75‑1 (MQ=255)
                                                                                                                                                                                                                              cTGGCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCGCTTTGGTTAACCCACCGAGCGCATGTTTAGCGGCTGTGTAGGCGCTGGCATCCGGCAGCGGCGTATGTTCATGTAc          <  2:202552/236‑1 (MQ=255)
                                                                                                                                                                                                                                 gCAATCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCGCTTTGGTTAACCCACCGAGCGCATGTTTAGCGGCTGTGTAGGCGCTGGCATCCGGCAGCGGCGTATGTTCATGTACCGACGt    >  2:992185/1‑239 (MQ=255)
                                                                                                                                                                                                                                   aaTCTCCTGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCGCTTTGGTTAACCCACCGAGCGCATGTTTAGCGGCTGTGTAGGCGCTGGCATCCGGCAGCGGCGTATGTTCATGTACCGACGTaa  <  2:78456/239‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                        
GATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAACGCCGCAAGGGAATCGAAGGCTCCGCGTCGGGCTTCACGTCGCTGTCATCCATGCCATTCATTGGCGTGGCGATCGCCCCAGGCGCGACTGCGTTCACCAAAATCTTATGCCTGACCAGCTCCAGCGCCATCGCTTTGGTTAACCCACCGAGCGCATGTTTAGCGGCTGTGTAGGCGCTGGCATCCGGCAGCGGCGTATGTTCATGTACCGACGTAA  >  NC_000913/2226393‑2226858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: