Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,982,326 T→G intergenic (‑144/+171) araE ← / ← kduD arabinose transporter/2‑dehydro‑3‑deoxy‑D‑gluconate 5‑dehydrogenase; KDG oxidoreductase; 20‑ketosteroid reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,982,3260TG98.1% 189.8 / ‑4.9 53intergenic (‑144/+171)araE/kduDarabinose transporter/2‑dehydro‑3‑deoxy‑D‑gluconate 5‑dehydrogenase; KDG oxidoreductase; 20‑ketosteroid reductase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (23/29);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

ACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTTGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCGGCAGGGTCATAAAAGTAAGAAGAATGAATTAACGCGCCAGCCAACCGCCATCCACGGCAATGGTATAACCATTCACA  >  NC_000913/2982091‑2982545
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                           
aCCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGaa                                                                                                                                                                                                                          >  1:564292/1‑239 (MQ=255)
        agcTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAattatt                                                                                                                                                                                                                     <  2:272831/236‑1 (MQ=255)
           tACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTgaga                                                                                                                                                                                                                 <  2:12517/237‑1 (MQ=255)
           tACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGat                                                                                                                                                                                                                <  1:1162042/238‑1 (MQ=255)
                       caTATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAacca                                                                                                                                                                                                    >  1:874614/1‑238 (MQ=255)
                         tatTCATACGCCTCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCt                                                                                                                                                                                                 <  2:249980/239‑1 (MQ=255)
                         tatTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCt                                                                                                                                                                                                 <  2:564292/239‑1 (MQ=255)
                                aCGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAattatt                                                                                                                                                                                                                     >  1:534090/1‑212 (MQ=255)
                                aCGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAattatt                                                                                                                                                                                                                     <  2:534090/212‑1 (MQ=255)
                                  gccgcGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTAca                                                                                                                                                                                        >  2:127156/1‑239 (MQ=255)
                                      cgTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGat                                                                                                                                                                                                                <  1:606529/211‑1 (MQ=255)
                                      cgTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGat                                                                                                                                                                                                                >  2:606529/1‑211 (MQ=255)
                                      cgTATCCCGCAAAGAACGTGGCGTTAAAGCACATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTAcagcag                                                                                                                                                                                    >  1:1111716/1‑239 (MQ=255)
                                                                          cGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGat                                                                                                                                                                                                                <  1:960374/175‑1 (MQ=255)
                                                                          cGTATTGAAAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGat                                                                                                                                                                                                                >  2:960374/1‑175 (MQ=255)
                                                                                   aGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGattatt                                                                                                                                       >  1:288099/1‑239 (MQ=255)
                                                                                         cATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATaa                                                                                                                                 <  2:927343/239‑1 (MQ=255)
                                                                                                tCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGat                                                                                                                          <  2:288099/239‑1 (MQ=255)
                                                                                                tCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGat                                                                                                                          <  1:127156/239‑1 (MQ=255)
                                                                                                  cTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAAAATGGATTATTTCATAACCATGatat                                                                                                                        <  1:143345/239‑1 (MQ=255)
                                                                                                     ccAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTgaga                                                                                                                                                                                                                 >  1:1113349/1‑147 (MQ=255)
                                                                                                     ccAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTgaga                                                                                                                                                                                                                 <  2:1113349/147‑1 (MQ=255)
                                                                                                                        aGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAattatt                                                                                                                                                                                                                     >  2:558024/1‑124 (MQ=255)
                                                                                                                        aGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAattatt                                                                                                                                                                                                                     <  1:558024/124‑1 (MQ=255)
                                                                                                                        aGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCt                                                                                                                                                                                                 <  2:972423/144‑1 (MQ=255)
                                                                                                                        aGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCt                                                                                                                                                                                                 >  1:972423/1‑144 (MQ=255)
                                                                                                                        aGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGat                                                                                                                          >  2:482684/1‑215 (MQ=255)
                                                                                                                        aGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGat                                                                                                                          <  1:482684/215‑1 (MQ=255)
                                                                                                                        aGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGta                                                                                                  >  1:318542/1‑239 (MQ=255)
                                                                                                                           gAAAAAATACGTGAACAACTCACGCAGGTGTCAAGTCGGAAACAGCATAAATATGGATTAAATTGCTGTGACAGGTAGTTATGTGATGGATATTCCAATTTTCAAATAAAGTTGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTACATTAATTGATAAAAAATATGGa                                                                                                                                            <  2:301536/194‑1 (MQ=255)
                                                                                                                                            acTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCgg                                                                              <  2:479312/239‑1 (MQ=255)
                                                                                                                                             cTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCGGc                                                                             <  2:318542/239‑1 (MQ=255)
                                                                                                                                                        gtCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAg                                                                                                     >  1:231590/1‑204 (MQ=255)
                                                                                                                                                        gtCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAg                                                                                                     <  2:231590/204‑1 (MQ=255)
                                                                                                                                                                                tGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCGGCAGGGTCATAAAAGTAAGAAGAATGAATTAAcgcg                                           >  1:400791/1‑238 (MQ=255)
                                                                                                                                                                                  gATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAg                                                                                                     >  1:400401/1‑178 (MQ=255)
                                                                                                                                                                                  gATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAg                                                                                                     <  2:400401/178‑1 (MQ=255)
                                                                                                                                                                                            ctgcGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCGGCAGGGTCATAAAAGTAAGAAGAATGAATTAACGCGCCAGCCAACCGcc                              >  1:489042/1‑239 (MQ=255)
                                                                                                                                                                                             tgcGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAattatt                                                                                                                                                                                                                     >  1:416150/1‑55 (MQ=255)
                                                                                                                                                                                             tgcGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAattatt                                                                                                                                                                                                                     <  2:416150/55‑1 (MQ=255)
                                                                                                                                                                                                      tCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCGGCAGGGTCATAAAAGTAAGAAGAATGAATTAACGCGCCAGCCAACCGCCATc                           <  2:1111716/232‑1 (MQ=255)
                                                                                                                                                                                                       cGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAACCCCTGCCATGCGGCAGGGTCATAAAAGTAAGAAGAATGAATTAACGCGCCAGCCAACCGCCATCCACGGCa                    <  2:400791/238‑1 (MQ=255)
                                                                                                                                                                                                       cGTTATGTGATGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCGGCAGGGTCATAAAAGTAAGAAGAATGAATTAACGCGCCAGCCAACCGCCATCCACGGCaa                   <  1:76581/239‑1 (MQ=255)
                                                                                                                                                                                                                aTGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCGGCAGGGTCATAAAAGTAAGAAGAATGAATTAACGCGCCAGCCAACCGCCATCCACGGc                     >  1:586464/1‑228 (MQ=255)
                                                                                                                                                                                                                aTGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCGGCAGGGTCATAAAAGTAAGAAGAATGAATTAACGCGCCAGCCAACCGCCATCCACGGc                     <  2:586464/228‑1 (MQ=255)
                                                                                                                                                                                                                aTGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCGGCAGGGTCATAAAAGTAAGAAGAATGAATTAACGCGCCAGCCAACCGCCATCCACGGCaa                   >  2:1175784/1‑230 (MQ=255)
                                                                                                                                                                                                                aTGGATATTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCGGCAGGGTCATAAAAGTAAGAAGAATGAATTAACGCGCCAGCCAACCGCCATCCACGGCaa                   <  1:1175784/230‑1 (MQ=255)
                                                                                                                                                                                                                    atatTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCGGCAGGGTCATAAAAGTAAGAAGAATGAATTAACGGGCCTCCCAACCGCCATCCACGGCAATGGTATAACCAt       <  2:91970/238‑1 (MQ=255)
                                                                                                                                                                                                                      atTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGat                                                                                                                          >  2:150119/1‑121 (MQ=255)
                                                                                                                                                                                                                      atTCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGat                                                                                                                          <  1:150119/121‑1 (MQ=255)
                                                                                                                                                                                                                        tCCAATTTTCAAATTAAGTGGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCGGCAGGGTCATAAAAGTAAGAAGAATGAATTAACGCGCCAGCCAACCGCCATCCACGGCAATGGTATAACCATTcaca  >  1:1044197/1‑239 (MQ=255)
                                                                                                                                                                                                                             ttttCAAATTAAGTGGAATTATTGAGATTATTATTaa                                                                                                                                                                                                       >  1:893470/1‑37 (MQ=255)
                                                                                                                                                                                                                             ttttCAAATTAAGTGGAATTATTGAGATTATTATTaa                                                                                                                                                                                                       <  2:893470/37‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                           
ACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTATTGATAGTAACCATTTTTTCCTGCCAGCAGAGAGTAAGACATAGTGAAAAAATACGTGAACAACTCACGCAGGTGTCAGGTCGGAAACAGCATAAATATGGATTAAATTGCTGCGACATGTCGTTATGTGATGGATATTCCAATTTTCAAATTAAGTTGAATTATTGAGATTATTATTAACCACCTAATTTTACAGCAGATAAAATTCATAAAGTTCATTAATTGATAATTAATATGGATTATTTCATAACCATGATATGGATTATGATGATCTACAGGTATAAAAAACCCTGCCATGCGGCAGGGTCATAAAAGTAAGAAGAATGAATTAACGCGCCAGCCAACCGCCATCCACGGCAATGGTATAACCATTCACA  >  NC_000913/2982091‑2982545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑