Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,499,6160GA34.2% 56.7 / 12.9 38intergenic (+1/‑294)yhfL/frlAsmall lipoprotein/putative fructoselysine transporter
Reads supporting (aligned to +/- strand):  ref base G (2/23);  new base A (13/0);  total (15/23)
Fisher's exact test for biased strand distribution p-value = 1.94e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCAT  >  NC_000913/3499437‑3499840
                                                                                                                                                                                   |                                                                                                                                                                                                                                            
aGGGAATGTAAATGAACAAATTTATTAATGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACAGTAAAATTCTGGCAGGAAAAAGCTAAAAACGGTTTAATTTGCCGAAGCCCCCTTGGttttt                                                                                                                                                                                  >  1:551707/1‑235 (MQ=255)
aGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGGGTTCTACTTCCCCCTAAATTTCTGAACGAAAACGGCTAACACCCGGTTTATTTTCCCACACCCTCTCGGttttt                                                                                                                                                                                  >  1:29838/1‑235 (MQ=255)
   gAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGa                                                                                                                                                                               <  1:425092/236‑1 (MQ=255)
      tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGGGGCCTACTGCCCCGTAAAACTCTGTCAGAAATCGGGTAACACCCGGTTATTTGCCCGACGGCCCCTCGCtttttcctagg                                                                                                                                                                            >  2:488750/1‑229 (MQ=255)
       ttAAATGAACAAATTTATTAAAGTTCCACTGGTAGGTGCAGTACTTGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAgg                                                                                                                                                                             <  1:379299/233‑1 (MQ=255)
              aaCAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTg                                                                                                                                                                   <  1:212229/236‑1 (MQ=255)
                                    tGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTg                                                                                                                                                                                         <  1:429484/193‑1 (MQ=255)
                                          gTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAac                                                                                                                                                                                                                        <  1:370107/160‑1 (MQ=255)
                                                tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCCACTGCACAGTAAATCTCTTACAAAAAATGGCTTAAACCCGTTTAATTTCCCGGCCGCCCCCCGCATTTTTCCGGGGGCATTTGTTTCTTTTTCCTTCTTTGCCCCCCCCGCaaaaac                                                                                                                             >  2:602681/1‑235 (MQ=255)
                                                                    tGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATAttt                                                                                                                        <  1:360202/220‑1 (MQ=255)
                                                                    tGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCCACTGCCCAGTAAATTTCTTCAAGAAAACGGCTAAAAACGGGTTTATTGCCCGCAGCCCCCTGGGTTTTTCCATGGGGGAGTTGTTTTTTTTTTCTTTTTTCCCCCGCCCCTCTAAAAATTTTCACTTTCAAtt                                                                                                            >  2:450145/1‑232 (MQ=255)
                                                                        cTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTGCTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGa                                                                                                    <  1:135434/236‑1 (MQ=255)
                                                                          ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGTAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCCACTGCACCGTAAATCTCTGACAGAAATAGGCCAACACCGGGTTTATTGTCCGCCCCCCCCTGGAGTTTTCGCGGGGGGGTTTTTTTCTTTTTCCCTCTTTTCTCCGCCCCTACAAAACTATTTTCCTTTAATTTCAAtttttt                                                                                                   >  2:694209/1‑233 (MQ=255)
                                                                          ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCCACTTCACAGGAAATCTCTg                                                                                                                                                                                                                                        >  2:370356/1‑112 (MQ=255)
                                                                          ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGGGGGCCCACTTCCCAATAAAATTCTTAAAAAAAACTGGTAAAACCCGGGTTATTTTCCCAGCCCCCCCTGTGTTTTCTCGGGCGTGTTTGTTTTTTTTTTCTTCTTTCTCCCCCCCCGTCAAACAATTTTACTTTTAATTTCACTTTgta                                                                                                   >  2:142832/1‑234 (MQ=255)
                                                                          ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                                                                   <  1:104374/235‑1 (MQ=255)
                                                                           gTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTACTGCCCAGTAAGTCTCTGACAGAAATCGGCCTAAACCGGGTTAATTGCCCGACGCCCTCTGGGTTTTTCGAGGGCGGATTTGTTTTTATTTTCTTTTTTTCCCCCCCCGTCCAAAACATTTTACttt                                                                                                                 >  2:360202/1‑220 (MQ=255)
                                                                           gTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTGCACAGTAAATCTCTGACAAAAAAAAGGTAACAACCGGTTTATTTTCCCGCCCCCCCCGGCATTTTTGTGGGCGGCTTTTTTTTTTTTTTCTTCTTTCCCCCCCCCCGTAAACAAAATTTCTCTTTAATTCCATTTtatat                                                                                                 >  2:251068/1‑235 (MQ=255)
                                                                            tCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTCCACACTAAATCTCTGAAAAAAAAAAGCCAAAACCGGGTTAATTGTCCGCCGCCCCCTTGGATTTTCGAAGGGGGGTTTTTTTCTATTTTCTTTTTTTCCCCGCCCGCTAAAATATTTTTACTCTTAATTCAAATTgtttat                                                                                                >  2:300684/1‑231 (MQ=255)
                                                                                atTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                             <  1:14004/235‑1 (MQ=255)
                                                                                               aaGAACTGCTCTTACGACTACCTGCTGCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                 <  1:694209/232‑1 (MQ=255)
                                                                                               aaGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                 <  1:642561/232‑1 (MQ=255)
                                                                                                     tGCTTTTACGACTACCTGCTGCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCCGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATc                                                                       <  2:91088/236‑1 (MQ=255)
                                                                                                                 tACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTACGGCCCTCACCCACAGTAAAACTCTTAACGAAAAAGCCCCACACCCGGTTTATTTCCCCACGCCCCCTTGGTTTTCCCCGGGCGTATTTTGTTGTCATGCTCTCCTTCCCCCCCCCGCACAAACATTTTTCCTTTTAATTTAAATTATTAACAACATCCATCCGCCACACCGCGAACCTATGCGa                                                             >  2:310606/1‑232 (MQ=255)
                                                                                                                      gctgcACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCCCAATAAATCTCTGACAGAAAACTGCTAACACCCGGTTTATTTCCCCCCGCCCTCTGGGTTTTTCCAGGGGGGGTTTGTTTTTTTTTCCCTCTTTTCCCCCCCCGCTAAAAAATTTTTACTTTTAATTTCATTTGTTTACTACATCCCTTTGCCACAGCGCGGTCCATTTGCGAAAtttttt                                                     >  2:149793/1‑234 (MQ=255)
                                                                                                                             ccGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGTCCACTGCACAGAAAATCTCTGTCACAAAAAAGCTCACACCGGCTTTATTTCCCGACCCCCCCTGGGTTTTCCGAGGGCGGATTTGTTTCTTTTTCCTTCTTTCCCCCGCCCCTAAAAAATATTTTACTTTTATATTAAATTTTTAATTACATCCCTTACACATAGTGCGATCCTGTGCTGAAATTTTTGATCttt                                              >  2:750840/1‑234 (MQ=255)
                                                                                                                                   atttctatttctAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                       <  1:450145/236‑1 (MQ=255)
                                                                                                                                    tttctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                       <  1:142832/235‑1 (MQ=255)
                                                                                                                                        tatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACa                                  <  1:79150/236‑1 (MQ=255)
                                                                                                                                        tatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACa                                  <  1:602681/236‑1 (MQ=255)
                                                                                                                                         atttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                 <  1:549470/236‑1 (MQ=255)
                                                                                                                                          tttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATa                                <  1:76201/236‑1 (MQ=255)
                                                                                                                                            tctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTGCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAc                              <  1:310606/236‑1 (MQ=255)
                                                                                                                                                  aTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCACGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                        <  1:747355/236‑1 (MQ=255)
                                                                                                                                                                    ccTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTcatcat      <  1:149793/236‑1 (MQ=255)
                                                                                                                                                                        cTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCAt  <  2:551707/236‑1 (MQ=255)
                                                                                                                                                                             caGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCt                                                                                                                                                              >  2:414090/1‑82 (MQ=255)
                                                                                                                                                                             caGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCt                                                                                                                                                              <  1:414090/82‑1 (MQ=255)
                                                                                                                                                                                   gTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCca                                         >  2:751097/1‑186 (MQ=255)
                                                                                                                                                                                   gTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCca                                         <  1:751097/186‑1 (MQ=255)
                                                                                                                                                                                   |                                                                                                                                                                                                                                            
AGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCAT  >  NC_000913/3499437‑3499840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: