Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,185,809 T→G intergenic (‑13/‑108) rcnR ← / → rcnA transcriptional repressor of rcnA/membrane protein conferring nickel and cobalt resistance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,185,8090TG96.5% 188.0 / ‑0.1 58intergenic (‑13/‑108)rcnR/rcnAtranscriptional repressor of rcnA/membrane protein conferring nickel and cobalt resistance
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (27/29);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.41e-01

TAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGAACCAGGGCACTCAAAAACGATGATGGCGGCGTTTA  >  NC_000913/2185581‑2186040
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        
tAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCCGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAg                                                                                                                                                                                                                                   >  2:143211/1‑236 (MQ=255)
      aTCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATg                                                                                                                                                                                                                             >  1:420904/1‑236 (MQ=255)
                 gAACGATGTTTTCCGTCAGATGACCTTTAATCAGTTCCCGCATCAGGCCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAg                                                                                                                                                                                                                  <  2:721605/236‑1 (MQ=255)
                 gAACGATGTGTTCCGTCAGATGACCTTTAATCCCTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAg                                                                                                                                                                                                                  <  2:634980/236‑1 (MQ=255)
                 gAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAg                                                                                                                                                                                                                  <  2:206074/236‑1 (MQ=255)
                 gAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAg                                                                                                                                                                                                                  <  2:420904/236‑1 (MQ=255)
                                 cAGATGCCCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTg                                                                                                                                                                                                  <  1:589357/236‑1 (MQ=255)
                                 cAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTg                                                                                                                                                                                                  <  1:487096/236‑1 (MQ=255)
                                        ccTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTCACAAGAATATGCAAAATCATCGACCTATTAATCTCCTtcggggca                                                                                                                                                                                           >  2:7115/1‑234 (MQ=255)
                                        ccTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgta                                                                                                                                                                                           <  1:55618/236‑1 (MQ=255)
                                             aaTCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATc                                                                                                                                                                                      <  1:623315/236‑1 (MQ=255)
                                                      ccGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATTTACTGGGGGGTAGTATCAGGTACTgg                                                                                                                                                                             >  1:95287/1‑236 (MQ=255)
                                                               aCCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTgggggg                                                                                                                                                                                             >  1:756196/1‑211 (MQ=255)
                                                               aCCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTgggggg                                                                                                                                                                                             <  2:756196/211‑1 (MQ=255)
                                                                         gcgcCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGCCCTGGCCCTGCATCTTACTGGCCCGCGCTTTCAGTTTCTGTTTATCCCGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATACTAGCACAATGAATCTACCTGGGGGGAGTAACAGGTACTTGGAGGGAGGTGAATCAGAtt                                                                                                                                                          >  2:234337/1‑236 (MQ=255)
                                                                           gcCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCCGGCCCTGAATCTTCCTGGCACGCGCTTTCAGTTTCTGTTTATACCGGATTGTATGAGACATGGCAACACCTGGGTTACAAGAATATGGAAAATTATAGCTCTATTAATCTACTGGGGGGTAGCATTAGGTACTCGCAGGGTGTCGGATCAGAtatc                                                                                                                                                        >  2:22371/1‑233 (MQ=255)
                                                                                  ctAGCAGCAGTCTGTGCTAAATATCCTACGCCTTCGTGGGTGTCTTTGAACACTTTCGTGACCTGCCCGACCAGGCCCTGACTCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACAAGGCAACCCCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTCCTGGGGGGTAGTATCAGGTTCTGGAGGATAGTACAATCAGATTGCCGAATTa                                                                                                                                                 <  2:661718/235‑1 (MQ=255)
                                                                                  aTAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCCTTTGCTTGATCTCCACGTCCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGTGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTa                                                                                                                                                 <  1:704059/236‑1 (MQ=255)
                                                                                       agcaATCTGTTGTAAAACTTCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACt                                                                                                                                            <  1:625117/236‑1 (MQ=255)
                                                                                            tCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGaa                                                                                                                                       >  1:350890/1‑236 (MQ=255)
                                                                                             cTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAg                                                                                                                                         <  2:678934/233‑1 (MQ=255)
                                                                                             cTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAg                                                                                                                                         <  1:496254/233‑1 (MQ=255)
                                                                                             cTGTTATAAAACTTGAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTTAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAg                                                                                                                                         <  2:95287/233‑1 (MQ=255)
                                                                                                    aaaaCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATc                                                                                                                               >  1:225069/1‑236 (MQ=255)
                                                                                                          gCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATTAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGTGGTCGTATCCGTTACTGGGGGGGGGTAGAATCAGCATGACGAATTAATACTCAGaataatgttatgtacc                                                                                                                         >  2:415767/1‑226 (MQ=255)
                                                                                                          gCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtagta                                                                                                                                                                                        >  2:161011/1‑173 (MQ=255)
                                                                                                          gCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtagta                                                                                                                                                                                        <  1:161011/173‑1 (MQ=255)
                                                                                                          gCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGAcc                                                                                                                         <  1:695937/236‑1 (MQ=255)
                                                                                                           cAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGTATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGCAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAGTTACTACTAAGAATTATTATCTAGACCg                                                                                                                        >  2:695937/1‑236 (MQ=255)
                                                                                                                        cGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAg                                                                                                                                                                                                                  <  1:658114/133‑1 (MQ=255)
                                                                                                                        cGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAg                                                                                                                                                                                                                  >  2:658114/1‑133 (MQ=255)
                                                                                                                            tcgtcgAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTcttctt                                                                                                       <  1:240028/236‑1 (MQ=255)
                                                                                                                             cgtctAGCATTTTCTTGAGCGCCACGACCTGGCCCTGCATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTcttctt                                                                                                       <  2:703315/235‑1 (MQ=255)
                                                                                                                             cgtcgAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGaaa                                                                                                                                                                                                                          <  1:599730/120‑1 (MQ=255)
                                                                                                                             cgtcgAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGaaa                                                                                                                                                                                                                          >  2:599730/1‑120 (MQ=255)
                                                                                                                             cgtcgAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTgg                                                                                                                                                                                                 <  1:173219/145‑1 (MQ=255)
                                                                                                                             cgtcgAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTgg                                                                                                                                                                                                 >  2:173219/1‑145 (MQ=255)
                                                                                                                                     aTTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCGAATTGCCGAATTAATTCTAAGAATTATTACCTTGACCGAATTTACAAACTTCCTTCACCAAgg                                                                                               >  2:459595/1‑235 (MQ=255)
                                                                                                                                                          tGGCCCTTAATCTTACTGGCACTAGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGGCATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGttct                                                                                 <  2:625770/228‑1 (MQ=255)
                                                                                                                                                            gCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCa                                                                        >  1:643480/1‑235 (MQ=255)
                                                                                                                                                                 gAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATccc                                                                          >  1:625770/1‑228 (MQ=255)
                                                                                                                                                                  aaTCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAg                                                                                                                                                                                                                  >  2:58110/1‑91 (MQ=255)
                                                                                                                                                                  aaTCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAg                                                                                                                                                                                                                  <  1:58110/91‑1 (MQ=255)
                                                                                                                                                                        aCTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACt                                                           <  1:7115/236‑1 (MQ=255)
                                                                                                                                                                              aCGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACCCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCACCAGCGCCATCTTACTTGGtc                                                      >  2:45087/1‑234 (MQ=255)
                                                                                                                                                                                   cTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGTAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGATCGCTTc                                                >  2:69053/1‑236 (MQ=255)
                                                                                                                                                                                     ttCAGTTTCTATTTATCACGGATTGTATGAGACATGGCAACGACTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCa                                               <  1:504185/235‑1 (MQ=255)
                                                                                                                                                                                              tttttATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCt                                         <  1:459595/230‑1 (MQ=255)
                                                                                                                                                                                              tGTTTATCACGGATTGTATGAGACATGGCAACGCCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCt                                         <  1:143211/232‑1 (MQ=255)
                                                                                                                                                                                              tGTTTATCACGGATTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCt                                         <  2:643480/232‑1 (MQ=255)
                                                                                                                                                                                                          aTTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggggg                                                                                                                                                                          <  2:584607/91‑1 (MQ=255)
                                                                                                                                                                                                          aTTGTATGAGACATGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggggg                                                                                                                                                                          >  1:584607/1‑91 (MQ=255)
                                                                                                                                                                                                                      aTGGCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAATCTTCTTCAGCAAGGAAACGCCTGCTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGATTCATGGCCCGGATACCATGCACTCACACAAgatgact            >  2:595767/1‑234 (MQ=255)
                                                                                                                                                                                                                        ggCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGACCTAGGGCACTCAAAAACGatgatgca          >  2:117974/1‑234 (MQ=255)
                                                                                                                                                                                                                        ggCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGAACCAGGGCa                            <  2:704112/218‑1 (MQ=255)
                                                                                                                                                                                                                        ggCAACACCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGAACCAGGGCa                            >  1:704112/1‑218 (MQ=255)
                                                                                                                                                                                                                             cacCTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGAACCAGGGCACTCAAAAACGATGATGGCGGCGt     >  1:163832/1‑236 (MQ=255)
                                                                                                                                                                                                                                cTGGGTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGAACCAGGGCACTCAAAAACGATGATGGCGCCGTTTa  >  1:499140/1‑236 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        
TAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGAACCAGGGCACTCAAAAACGATGATGGCGGCGTTTA  >  NC_000913/2185581‑2186040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: