Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,273,796 A→C D478A (GAC→GCC)  yejA → microcin C ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,273,7960AC98.3% 191.0 / ‑3.5 59D478A (GAC→GCC) yejAmicrocin C ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (29/29);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01

TGACAACCTGTTAAAAGCCGACAAACTTCTCAACGAAGCGGGCTGGGTGCTGAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGACTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGCGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCA  >  NC_000913/2273563‑2274028
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           
tGACAACCTGTTAAAAGCCGACAAACTTCTCAACGAAGCGGGCTGGGTGCTGAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCt                                                                                                                                                                                                                                           >  1:353160/1‑236 (MQ=255)
tGACAACCTGTTAAAAGCCGACAAACTTCTCAACGAAGCGGGCTGGGTGCTGAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGCGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAGCATGGCCATTCGCAAGGTGGATCACTCGGAAATCACTCACCGCATACGCACACGCGCCt                                                                                                                                                                                                                                           >  2:486524/1‑236 (MQ=255)
  aCAACCTGTTAAAAGCCGACAAACTTCTCAACGAAGCGGGCTGGGTGCTGAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGCTAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTAt                                                                                                                                                                                                                                         >  1:234258/1‑236 (MQ=255)
            aaaaGCCGACACACTTCGCAACGAAGCGGGCTGGGTGCTGAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGc                                                                                                                                                                                                                               <  1:736115/236‑1 (MQ=255)
                          ttCTCAACGAAGCGGGCTGGGTGCTGAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATg                                                                                                                                                                                                                                        <  1:504758/213‑1 (MQ=255)
                            ctcAACGAAGCGGGCTGGGTGCTGAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATggcg                                                                                                                                                                                                                  >  1:758373/1‑233 (MQ=255)
                               aaCGAAGCGGGCTGGGTGCTGAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATCACTCGCAAATCACTAACCGCATTCGCAGTCGCGCCTATGACATGATTACGCGCGGATTGCGGTCGAt                                                                                                                                                                                                            >  2:39321/1‑236 (MQ=255)
                                 cGAAGCGGGCTGGGTGCTGAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTa                                                                                                                                                                                                                                          <  2:756830/204‑1 (MQ=255)
                                 cGAAGCGGGCTGGGTGCTGAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTa                                                                                                                                                                                                                                          >  1:756830/1‑204 (MQ=255)
                                   aaGCGGGCTGGGTGCTTAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCg                                                                                                                                                                                                        <  1:521874/236‑1 (MQ=255)
                                   aaGCGGGCTGGGTGCTGAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCg                                                                                                                                                                                                        <  2:331384/236‑1 (MQ=255)
                                      cGGGCTGGGTGCTGAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCg                                                                                                                                                                                                        <  2:353160/233‑1 (MQ=255)
                                                   gAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGAtt                                                                                                                                                                                        <  1:429285/236‑1 (MQ=255)
                                                         tCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTa                                                                                                                                                                                                                                          >  1:448868/1‑180 (MQ=255)
                                                         tCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTa                                                                                                                                                                                                                                          <  2:448868/180‑1 (MQ=255)
                                                         tCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGGCATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGCttttaac                                                                                                                                                                                   >  1:4004/1‑231 (MQ=255)
                                                           aGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGCTGCCGGGGCCCAGTTCCCACTTTCAgc                                                                                                                                                                                  >  2:654449/1‑233 (MQ=255)
                                                                cgcgTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTg                                                                                                                                                                           <  2:625726/236‑1 (MQ=255)
                                                                 gcgTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGGGATGGCGTGGTCCAGGTTCGCATTACAGATATcacgg                                                                                                                                                                          >  2:232462/1‑232 (MQ=255)
                                                                 gcgTTAATGCCACAACGGGTAAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTg                                                                                                                                                                           <  1:323479/235‑1 (MQ=255)
                                                                                    tCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAAtt                                                                                                                                                      <  1:654449/236‑1 (MQ=255)
                                                                                            tCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAAtt                                                                                                                                                      >  1:480113/1‑228 (MQ=255)
                                                                                            tCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTtat                                                                                                                                              <  2:758373/236‑1 (MQ=255)
                                                                                            tCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCAGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAAtt                                                                                                                                                      <  2:480113/228‑1 (MQ=255)
                                                                                                    caaTTAGTGCTTCCCTCAAGCAGCAAGAGTCAGTGGGTACTCCCGTTCAAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACAGTATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATaa                                                                                                                                            <  2:203505/229‑1 (MQ=255)
                                                                                                          ttGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGCTTTTCACATTTCCTGTTGATCGGCATATATCAATTCCCCTTATAATTCACCCGGCgg                                                                                                                                >  2:627893/1‑235 (MQ=255)
                                                                                                          ttGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGaa                                                                                                                                                                <  2:575026/204‑1 (MQ=255)
                                                                                                          ttGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGGTCCGATTTACAGATTTCCTGGTCATCGGaa                                                                                                                                                                >  1:575026/1‑204 (MQ=255)
                                                                                                               tCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGGGGGCCAGGTCCGGTTTTCAGAGTTCCTTGTCAATCGGAATATATCAATTACCCTACAAATGCCCCCGGGTTGCa                                                                                                                            >  2:675387/1‑235 (MQ=255)
                                                                                                                  cGCAAGCAGCAATAGGCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGcc                                                                                                                       <  1:627893/236‑1 (MQ=255)
                                                                                                                             aTAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCCGTTCCGACTTACAGATTTTCTGGTCATCGGAGAATATCAATTCCACTTATCATGCCCCCAGCGCTGCAAAACCCgtctatgg                                                                                                              >  2:499755/1‑228 (MQ=255)
                                                                                                                                 tCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCCGCGTATGGCGGGCGATGCCGTGGCCCAGTTTCGATTTACa                                                                                                                                                                                    >  2:379354/1‑161 (MQ=255)
                                                                                                                                 tCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACa                                                                                                                                                                                    <  1:379354/161‑1 (MQ=255)
                                                                                                                                         tATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGACTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTg                                                                                                      <  1:67900/230‑1 (MQ=255)
                                                                                                                                          aTTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGGCATCGGAATATATCAATTCCACTTATAATGCCCCCCGCGTGCAAAGCCCGGCTATCGACTCGCTGATCAccc                                                                                               >  1:454789/1‑236 (MQ=255)
                                                                                                                                           ttGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCa                                                                                              >  1:336794/1‑236 (MQ=255)
                                                                                                                                           ttGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGGGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGAATCGCTGATCAACCa                                                                                              >  1:422824/1‑236 (MQ=255)
                                                                                                                                                            ccTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACANNATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAACATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAgg                                                                            >  1:58765/1‑236 (MQ=255)
                                                                                                                                                                               aaCATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGGATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGGTTTCGACTCGCTGATTCACCTAATTCTTTCCGCGCAGGGGAATTCAAGAAAATTTat                                                          >  2:745882/1‑235 (MQ=255)
                                                                                                                                                                                aCATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGGGCAAAGCCCGGTTTTCGACTCGCTGCTCAACCAAATTATTGCCGCGCCGGGAAATAAAGAAAAATTACtt                                                        >  2:220670/1‑235 (MQ=255)
                                                                                                                                                                                           cGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACt                                                                                                           <  2:685711/175‑1 (MQ=255)
                                                                                                                                                                                           cGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACt                                                                                                           >  1:685711/1‑175 (MQ=255)
                                                                                                                                                                                           cGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGc                                              <  1:675387/235‑1 (MQ=255)
                                                                                                                                                                                              aaGGTAGATAGCGCGCAAATCACTAACCGCATGCGCAGTCGCGCTTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGc                                              <  2:287399/232‑1 (MQ=255)
                                                                                                                                                                                                    gATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCCGGTCCGATTTAAAGATTTCCTGGTCATCGGAAAAAATAAATTCCACTTATAATGCCCCCGGCGTGTAAAGGCCGGGTTTACAACCGCTGGTCAACCCAATTATGTCACCGCCGGGGATAAAAGAAACATTACGTACGTTgggggtgagcccgga                                    >  2:61046/1‑225 (MQ=255)
                                                                                                                                                                                                      caaCTCGCAACTCACTAACCTGATGCCAAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTNNNNNGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGCGAGCACTGGa                                    <  2:58765/233‑1 (MQ=255)
                                                                                                                                                                                                            gCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCAt                                                                                                                                                                     >  1:434747/1‑101 (MQ=255)
                                                                                                                                                                                                            gCAAATCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCAt                                                                                                                                                                     <  2:434747/101‑1 (MQ=255)
                                                                                                                                                                                                                 tCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGTCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGCGAGCACTGGATCGCGTATTAACg                       <  1:61046/236‑1 (MQ=255)
                                                                                                                                                                                                                 tCACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGCGAGCACTGGATCGCGTATTAACg                       <  1:499755/236‑1 (MQ=255)
                                                                                                                                                                                                                 tCACTAACCGAATGAGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGCGAGCACTGGATCGCGTATTAACg                       <  1:745882/236‑1 (MQ=255)
                                                                                                                                                                                                                  cACTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGCGAGCACTGGATCGCGTATTAACGt                      <  1:133455/236‑1 (MQ=255)
                                                                                                                                                                                                                    cTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGc                                                                                                                                  >  2:614080/1‑128 (MQ=255)
                                                                                                                                                                                                                    cTAACCGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGc                                                                                                                                  <  1:614080/128‑1 (MQ=255)
                                                                                                                                                                                                                        ccGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTc                                                                                                                                                     >  2:152186/1‑105 (MQ=255)
                                                                                                                                                                                                                        ccGCATGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTc                                                                                                                                                     <  1:152186/105‑1 (MQ=255)
                                                                                                                                                                                                                             tGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCCGTTCCGATTTACAGATTTCCTGGGCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCCCTGATCAACCAAATTATTGCCGCCGCGGGACATAAAGAAAAATAAATGCCGTTAGGGCGCGCACTGGAACGCGGATTAACCCGGAAttatta            >  2:259968/1‑235 (MQ=255)
                                                                                                                                                                                                                             tGCGCAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATGTATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGCGAGCACTGGATCGCGTATTAACGTGGAAttatta            >  2:626465/1‑235 (MQ=255)
                                                                                                                                                                                                                              gcgcAGTCGCGCCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGCGAGCACTGGATCGCGTATTAACGTGGAATTATTACa          <  1:232462/236‑1 (MQ=255)
                                                                                                                                                                                                                                      gcgcCTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGCGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCa  <  1:220670/236‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           
TGACAACCTGTTAAAAGCCGACAAACTTCTCAACGAAGCGGGCTGGGTGCTGAAGGGTCAGCAACGCGTTAATGCCACAACGGGTCAGCCACTCAGCTTTGAATTATTGCTTCCCGCAAGCAGCAATAGTCAGTGGGTATTGCCGTTCCAGCACAGCCTGCAACGGCTGGGTATCAACATGGACATTCGCAAGGTGGATAACTCGCAAATCACTAACCGCATGCGCAGTCGCGACTATGACATGATGCCGCGCGTATGGCGGGCGATGCCGTGGCCCAGTTCCGATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGCGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCA  >  NC_000913/2273563‑2274028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: