Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,112	A→C	I161L (ATC→CTC)	yejH →	putative ATP‑dependent DNA or RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,112	0	A	C	100.0%	271.1 / NA	76	I161L (ATC→CTC)	yejH	putative ATP‑dependent DNA or RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/45);  total (31/45)

GCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACA  >  NC_000913/2280883‑2281335                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                gcgcAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTAc                                                                                                                                                                                                                            <  1:755165/235‑1 (MQ=255)     aaaGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCACAGGCTGGCTCTAACAGtt                                                                                                                                                                                                                       >  2:144383/1‑236 (MQ=255)      aaGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTAcctttgc                                                                                                                                                                                                                      >  2:153921/1‑231 (MQ=255)               aaggtaatggtgTATTTGGCAGCGTTCAGTTTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCa                                                                                                                                                                                                              <  2:26202/224‑1 (MQ=255)                 ggTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACgg                                                                                                                                                                                                           >  1:404643/1‑236 (MQ=255)                       gTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCa                                                                                                                                                                                                         <  2:671405/232‑1 (MQ=255)                           tATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTAcgc                                                                                                                                                                                                 >  2:429041/1‑236 (MQ=255)                            aTTTGGCAGCGTGCAGTCTGTCGCCCGTAACCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTAcg                                                                                                                                                                                                  <  1:576882/234‑1 (MQ=255)                             tttGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACgcgg                                                                                                                                                                                               >  1:738416/1‑236 (MQ=255)                                     cGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTAcg                                                                                                                                                                                                  <  2:470201/225‑1 (MQ=255)                                     cGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTAcg                                                                                                                                                                                                  >  1:470201/1‑225 (MQ=255)                                       tGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGaa                                                                                                                                                                                      >  1:153199/1‑235 (MQ=255)                                         cAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATgaga                                                                                                                                                                                       <  1:458345/232‑1 (MQ=255)                                         cAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGc                                                                                                                                                                                   <  1:97100/236‑1 (MQ=255)                                           gTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTTAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGccc                                                                                                                                                                                 <  1:539546/236‑1 (MQ=255)                                            tCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGccc                                                                                                                                                                                 >  1:540368/1‑235 (MQ=255)                                              tGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGc                                                                                                                                                                                                          <  2:317017/208‑1 (MQ=255)                                              tGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGc                                                                                                                                                                                                          >  1:317017/1‑208 (MQ=255)                                                  gCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTC‑ACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGt                                                                                                                                                                         <  1:190741/236‑1 (MQ=255)                                                   cccGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGt                                                                                                                                                                         <  1:411822/236‑1 (MQ=255)                                                      gTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTg                                                                                                                                                                        >  1:156337/1‑234 (MQ=255)                                                      gTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGAc                                                                                                                                                                      <  1:656706/236‑1 (MQ=255)                                                       tAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACt                                                                                                                                                                     <  1:292401/236‑1 (MQ=255)                                                        aaTCTTGATGCCTATGAGGGTTATTTTTCGCTGTTGATTGTCGATGAATTTCACCGTAATGGTAGCGATGAGGAGAGCCAATAGCAGCAACTCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTg                                                                                                                                                                    <  2:716803/236‑1 (MQ=255)                                                          tCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCa                                                                                                                                                                  >  1:764856/1‑236 (MQ=255)                                                              gATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGa                                                                                                                                                                       <  1:500563/227‑1 (MQ=255)                                                              gATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGa                                                                                                                                                                       >  2:500563/1‑227 (MQ=255)                                                                    tgtgagtgTGAATTTGCGATGTAGATTGGCGATGAATGTCACCGTATTGGTGACGATGAAGAGACCCAGTATCAGCAAATCCTCGCTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGc                                                                                                                                                         <  2:442983/228‑1 (MQ=255)                                                                      tCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGACAAAGCCCTTTTCCGTGACTGACTTTATGAACTGc                                                                                                                                                      >  1:201334/1‑236 (MQ=255)                                                                        aGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCg                                                                                                                                                    <  2:764856/236‑1 (MQ=255)                                                                              aaTTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTg                                                                                                                                                 <  1:722034/233‑1 (MQ=255)                                                                              aaTTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCg                                                                                                                                               <  1:424139/235‑1 (MQ=255)                                                                                ttttCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTta                                                                                                                                            <  1:429041/236‑1 (MQ=255)                                                                                 tttCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTta                                                                                                                                            <  1:237261/235‑1 (MQ=255)                                                                                 tttCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTta                                                                                                                                            <  2:153199/235‑1 (MQ=255)                                                                                             ttGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTACGAGCTGCCGCTGCGTTATATGATTAAacac                                                                                                                               <  2:738416/236‑1 (MQ=255)                                                                                                   aTGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACgg                                                                                                                             >  2:600476/1‑232 (MQ=255)                                                                                                         gTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGa                                                                                                                     <  2:540368/234‑1 (MQ=255)                                                                                                                tATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCa                                                                                                             >  2:717243/1‑235 (MQ=255)                                                                                                                     gttaCGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGa                                                                                                       <  1:641398/233‑1 (MQ=255)                                                                                                                     gTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGa                                                                                                       <  1:717243/236‑1 (MQ=255)                                                                                                                          gATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGCTTAAACACGGCTATCTGCCGCAGCCAGACCGACTgg                                                                                                   >  2:173613/1‑235 (MQ=255)                                                                                                                          gATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGa                                                                                                  <  1:600476/236‑1 (MQ=255)                                                                                                                           aTGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGa                                                                                                  >  2:316707/1‑235 (MQ=255)                                                                                                                            tGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGGCGCCAGAACGACTggct                                                                                                 >  1:750220/1‑233 (MQ=255)                                                                                                                            tGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGat                                                                                                 >  2:616735/1‑235 (MQ=255)                                                                                                                               agagagCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGc                                                                                             >  1:695681/1‑236 (MQ=255)                                                                                                                                    gCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTtata                                                                                                                                          <  2:5320/186‑1 (MQ=255)                                                                                                                                    gCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTtata                                                                                                                                          >  1:5320/1‑186 (MQ=255)                                                                                                                                    gCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAAAACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCagta                                                                                        >  1:589506/1‑236 (MQ=255)                                                                                                                                     ccAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGGTATATGATTAAACACTGCTATCTGACGCCGCCAGAACGACTGGATATGCCagtag                                                                                       >  1:215977/1‑236 (MQ=255)                                                                                                                                              gCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGCAGTGCACTACg                                                                              >  1:476829/1‑236 (MQ=255)                                                                                                                                                        actcacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCagta                                                                                        <  2:186151/216‑1 (MQ=255)                                                                                                                                                        actcacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCagta                                                                                        >  1:186151/1‑216 (MQ=255)                                                                                                                                                        actcacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCg                                                                    <  1:137893/236‑1 (MQ=255)                                                                                                                                                              cTGACAAAAGTGAATCCCCCCTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCag                                                                                          <  2:36228/208‑1 (MQ=255)                                                                                                                                                              cTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCag                                                                                          >  1:36228/1‑208 (MQ=255)                                                                                                                                                                gACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAgg                                                            <  1:316707/236‑1 (MQ=255)                                                                                                                                                                 aCAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGc                                                           <  1:616735/236‑1 (MQ=255)                                                                                                                                                                  caaagttGAATCCCACCTGACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGca                                                          <  2:589506/230‑1 (MQ=255)                                                                                                                                                                  cAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGca                                                          <  2:156337/236‑1 (MQ=255)                                                                                                                                                                    aaaGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTATACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGcaca                                                        <  1:144383/236‑1 (MQ=255)                                                                                                                                                                        tGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCg                                                                                                                                                    >  2:751340/1‑140 (MQ=255)                                                                                                                                                                        tGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCg                                                                                                                                                    <  1:751340/140‑1 (MQ=255)                                                                                                                                                                           acccccACTGCCGCTTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGATGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAAc                                                 <  2:404643/234‑1 (MQ=255)                                                                                                                                                                               ccACTTACGCCTGCTGGGGCTGACTGCCAAGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACggg                                              <  1:351837/235‑1 (MQ=255)                                                                                                                                                                                      cGCCTGCTGGGGCTGCCGACCACGCCTTTGCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAg                                      <  2:127664/236‑1 (MQ=255)                                                                                                                                                                                        ccgtctgGGCCTGACTGCCACGCCTTTGCGTTTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCg                                    <  2:695681/232‑1 (MQ=255)                                                                                                                                                                                           gctgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCag                                                                                          >  1:664648/1‑179 (MQ=255)                                                                                                                                                                                           gctgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCag                                                                                          <  2:664648/179‑1 (MQ=255)                                                                                                                                                                                            ctgGGGCTGACTGCCACGCCTTTGCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAg                                 <  1:173613/235‑1 (MQ=255)                                                                                                                                                                                                     aCTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAATCCGagc                           >  2:355087/1‑230 (MQ=255)                                                                                                                                                                                                                         gggCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacacc   >  1:220506/1‑234 (MQ=255)                                                                                                                                                                                                                          ggCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaaca  >  1:104571/1‑236 (MQ=255)                                                                                                                                                                                                                          ggCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaaca  <  1:501823/236‑1 (MQ=255)                                                                                                                                                                                                                          ggCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaaca  <  2:220506/236‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                GCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACA  >  NC_000913/2280883‑2281335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑