Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,292,894	A→C	L24R (CTG→CGG)	dsbE ←	periplasmic thioredoxin of cytochrome c‑type biogenesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,292,894	0	A	C	100.0%	101.8 / NA	31	L24R (CTG→CGG)	dsbE	periplasmic thioredoxin of cytochrome c‑type biogenesis
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/17);  total (14/17)

CGCAGAAAGCTGATTCAGATATTGATGTTCCGCACGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCAGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTCCGCCCAACGCCATCATCAGCCCGCCCGCCCAAATCCAGCGAACAAATGGTTTGTAGTAAA  >  NC_000913/2292680‑2293094                                                                                                                                                                                                                       |                                                                                                                                                                                                         cgcAGAAAGCTGATTCAGATATTGATGTTCCGCACGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTAAGCGCCCGCTGCCACAGAAccgcgtcgg                                                                                                                                                                                     >  2:5022/1‑231 (MQ=255)  gcAGAAAGCTGATTCAGATATTGATGTTCCGCACGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGc                                                                                                                                                                                    <  2:105476/236‑1 (MQ=255)      aaaGCTGATTCAGATATTGATGTTCCGCACGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATc                                                                                                                                                                                >  1:710662/1‑236 (MQ=255)          gttaatCAGATTGTGATGTTCCGCCAGGCAGTTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGc                                                                                                                                                                              <  2:610887/229‑1 (MQ=255)              ttCAGATCTTGATGTTCCGCACGGCAGGTCGGACCCCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCa                                                                                                                                                                            <  2:740790/232‑1 (MQ=255)              ttCAGATATGGATGGTCCGCAGGGCAGGTCGGACCCCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCa                                                                                                                                                                            <  1:268674/232‑1 (MQ=255)                           gTTCCGGTCGGCAGGCCGGACCCCAGGTCGGCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATAACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGa                                                                                                                                                           <  2:710662/236‑1 (MQ=255)                           gTTCCGCACGGAAGGTCGGACACCAGGTCGCCCGGACGTTAAGCAGTACTGGTTTGCCCTGAGTCATCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGa                                                                                                                                                           <  2:454763/236‑1 (MQ=255)                             tCCGCACGGCAGGTCGGACCCCAGTCCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAAt                                                                                                                                                         <  2:352447/236‑1 (MQ=255)                              ccGCACGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGGGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAAtt                                                                                                                                                        >  1:225353/1‑236 (MQ=255)                                  aCGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACa                                                                                                                                                    <  2:366427/236‑1 (MQ=255)                                                            aGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGTGCTTCATACGGCCTcc                                                                                                                           >  2:67934/1‑235 (MQ=255)                                                                             ggTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCg                                                                                                           <  1:5022/234‑1 (MQ=255)                                                                                   cccTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGTCTCCGGCGCAGGTTTTTGTGGACTCa                                                                                                     >  2:691856/1‑234 (MQ=255)                                                                                             gCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGgc                                                                                                                        >  2:148782/1‑205 (MQ=255)                                                                                             gCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGgc                                                                                                                        <  1:148782/205‑1 (MQ=255)                                                                                                  tCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGa                                                                                    <  1:67934/236‑1 (MQ=255)                                                                                                                    cccGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGTTAGCGAGGGTCaaaacgac                                                                       >  2:747110/1‑226 (MQ=255)                                                                                                                        gATTGTCCAGTTATTCGAGACCAAACTTCGGCACAGGCTTGCCAATGAGCGCCGAGTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTc                                                              <  1:747110/236‑1 (MQ=255)                                                                                                                                  tGATTCGAAACCAAGCTGCGGCCCAGGCTTGCCAATGAGCCCCGATTCCAGATGGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTCCGCCCAACGc                                                    <  2:663952/236‑1 (MQ=255)                                                                                                                                       cGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGGGCTTCATACGGCCTCCGGCGAGGTTTTTTGCGGACTCACGCGCTTACGATAACGAGGATCAAACCGCCACAGCAGTCGGCCCAACGccgta                                                >  2:636839/1‑232 (MQ=255)                                                                                                                                         agaCGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACAcag                                                                   <  2:548833/214‑1 (MQ=255)                                                                                                                                         agaCGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACAcag                                                                   >  1:548833/1‑214 (MQ=255)                                                                                                                                            cGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGGTTTTTGCGGACTCACTCGCTTACGATAGCGAGGCTCAAACAGACACAGCAGTTCGCCCAACGCCATCATCAg                                           >  2:150548/1‑235 (MQ=255)                                                                                                                                                            gCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGa                                                                                    <  2:284119/178‑1 (MQ=255)                                                                                                                                                            gCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGa                                                                                    >  1:284119/1‑178 (MQ=255)                                                                                                                                                                 ccAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTCCGCCCAACGCCATCATCAGCCCGACCGCCCCAATCCAGCgc                     >  2:744169/1‑235 (MQ=255)                                                                                                                                                                      gAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCAc                                                                                                    >  2:657847/1‑152 (MQ=255)                                                                                                                                                                      gAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCAc                                                                                                    <  1:657847/152‑1 (MQ=255)                                                                                                                                                                           ccGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTCCGCCCAACGCCATCATCAGCCCGCCCGCCCAAATCCAGCGAACAAATGGt            <  1:744169/235‑1 (MQ=255)                                                                                                                                                                                     aTTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTCCGCCCAACGCCATCATCAGCCCGCCCGCCCAAATCCAGCGAACAAATGGTTTGTAGTaaa  >  2:375321/1‑235 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                         CGCAGAAAGCTGATTCAGATATTGATGTTCCGCACGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCAGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTCCGCCCAACGCCATCATCAGCCCGCCCGCCCAAATCCAGCGAACAAATGGTTTGTAGTAAA  >  NC_000913/2292680‑2293094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑