Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,387,305	G→A	P124P (CCG→CCA)	yfbM →	DUF1877 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,387,305	0	G	A	100.0%	280.4 / NA	72	P124P (CCG→CCA)	yfbM	DUF1877 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/40);  total (32/40)

TTTACACTTTGGGCTGACAGGCACCTCTGCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCC  >  NC_000913/2387077‑2387533                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                     tttACACTTTGGGCTGACAGGCACCTCTGCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGa                                                                                                                                                                                                                               >  1:263510/1‑236 (MQ=255)  ttACACTTTGGGCTGACAGGCACCTCTGCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGAc                                                                                                                                                                                                                              <  2:117339/236‑1 (MQ=255)          tGGGCTGACAGGCACCTCTGCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCag                                                                                                                                                                                                                      >  1:563031/1‑236 (MQ=255)           gggCTGACAGGCACCTCTGCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCaga                                                                                                                                                                                                                     <  1:260482/236‑1 (MQ=255)             gCTGACAGGCACCTCTGCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTcagcag                                                                                                                                                                                                                   >  1:295261/1‑236 (MQ=255)                                     tCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCt                                                                                                                                                                                           <  1:624524/236‑1 (MQ=255)                                                   aTCCGATGAGTCGTGCCGTTCTTGGTAAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGa                                                                                                                                                                              <  2:478868/235‑1 (MQ=255)                                                            gTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAg                                                                                                                                                                    <  1:454461/236‑1 (MQ=255)                                                                tgccgtgcTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGa                                                                                                                                                                >  1:413354/1‑236 (MQ=255)                                                                          ggTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTa                                                                                                                                                      >  2:741368/1‑236 (MQ=255)                                                                                  caGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAAATCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACg                                                                                                                                                >  2:270943/1‑234 (MQ=255)                                                                                               ggCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAgg                                                                                                                                 >  1:105094/1‑236 (MQ=255)                                                                                               ggCATAGATGGCTTCATTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTTCGACAAgg                                                                                                                                 >  2:585638/1‑236 (MQ=255)                                                                                                 cATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAgg                                                                                                                                 <  2:245224/234‑1 (MQ=255)                                                                                                   tAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGc                                                                                                                                                                                                       <  2:44445/162‑1 (MQ=255)                                                                                                   tAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGc                                                                                                                                                                                                       >  1:44445/1‑162 (MQ=255)                                                                                                          cTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATgcgc                                                                                                                      >  1:621400/1‑236 (MQ=255)                                                                                                          cTTCCTTGGATTGACATGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATgcg                                                                                                                       <  1:131617/235‑1 (MQ=255)                                                                                                           ttCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCt                                                                                                                     >  1:737145/1‑236 (MQ=255)                                                                                                              cTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGAc                                                                                                                  <  2:621400/236‑1 (MQ=255)                                                                                                               ttGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGAc                                                                                                                  <  2:222897/235‑1 (MQ=255)                                                                                                                       aCCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTa                                                                                                                                                      >  1:165638/1‑191 (MQ=255)                                                                                                                       aCCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTa                                                                                                                                                      <  2:165638/191‑1 (MQ=255)                                                                                                                       aCCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTAttgt                                                                                                         >  2:70557/1‑236 (MQ=255)                                                                                                                       aCCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTAttgt                                                                                                         >  1:474212/1‑236 (MQ=255)                                                                                                                       aCCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTAttgt                                                                                                         >  1:34184/1‑236 (MQ=255)                                                                                                                         cTGGAATCAGGATTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTAttgttg                                                                                                       <  2:178429/236‑1 (MQ=255)                                                                                                                          tGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTCTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTgg                                                                                                      <  2:423667/236‑1 (MQ=255)                                                                                                                            gAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGt                                                                                                    >  2:228663/1‑236 (MQ=255)                                                                                                                             aaTCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTg                                                                                                   <  1:70557/236‑1 (MQ=255)                                                                                                                                 aGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGaa                                                                                                                                                                       >  2:355867/1‑164 (MQ=255)                                                                                                                                 aGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGaa                                                                                                                                                                       <  1:355867/164‑1 (MQ=255)                                                                                                                                  ggAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGAc                                                                                                                                                                                                                              >  1:268014/1‑108 (MQ=255)                                                                                                                                  ggAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGAc                                                                                                                                                                                                                              <  2:268014/108‑1 (MQ=255)                                                                                                                                       tAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGata                                                                                                <  2:474212/229‑1 (MQ=255)                                                                                                                                              gACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGcc                                                                                    <  1:658617/234‑1 (MQ=255)                                                                                                                                                  aTCGGCCGACTAGAATCACTGGATCGAAATGAACTGCGTAGACAGTTTTCCATTAAGCGACTAAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTgggg                                                                               <  2:167136/235‑1 (MQ=255)                                                                                                                                                      accgacTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGaa                                                                          <  1:423084/236‑1 (MQ=255)                                                                                                                                                      accgacTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGaa                                                                          <  2:105094/236‑1 (MQ=255)                                                                                                                                                      accgacTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGaa                                                                          <  1:675428/236‑1 (MQ=255)                                                                                                                                                       ccgacTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGaaa                                                                         <  1:424719/236‑1 (MQ=255)                                                                                                                                                         gacTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGa                                                                       <  1:270943/236‑1 (MQ=255)                                                                                                                                                            tGGAATCACTGGATCGAAATGAAATGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTGGATGCCTGGGGGGGACAGAccc                                                                    >  2:652846/1‑236 (MQ=255)                                                                                                                                                             ggAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCa                                                                   <  1:741368/236‑1 (MQ=255)                                                                                                                                                              gAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCAt                                                                  >  2:647730/1‑236 (MQ=255)                                                                                                                                                                  cACTGGATCGAAATGGTCTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGATATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCa                                                                   <  1:333473/231‑1 (MQ=255)                                                                                                                                                                  cACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCAt                                                                  <  2:214812/232‑1 (MQ=255)                                                                                                                                                                        aTCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGa                                                         >  1:343554/1‑235 (MQ=255)                                                                                                                                                                         tCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCt                                                                                                                     <  1:302255/174‑1 (MQ=255)                                                                                                                                                                         tCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCt                                                                                                                     >  2:302255/1‑174 (MQ=255)                                                                                                                                                                         tCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGAtt                                                       <  2:263510/236‑1 (MQ=255)                                                                                                                                                                                      cGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGatca                                          >  1:442129/1‑236 (MQ=255)                                                                                                                                                                                      cGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATCCGCTGACGGTTATTGTTGGGGGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAGACTTTACGCCAGGa                                             >  2:89306/1‑233 (MQ=255)                                                                                                                                                                                                tttCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTa                                                                                                                                                      <  1:489405/118‑1 (MQ=255)                                                                                                                                                                                                tttCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTa                                                                                                                                                      >  2:489405/1‑118 (MQ=255)                                                                                                                                                                                                tttCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGt                                                                                                                                               >  2:525723/1‑125 (MQ=255)                                                                                                                                                                                                tttCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGt                                                                                                                                               <  1:525723/125‑1 (MQ=255)                                                                                                                                                                                                tttCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATgcg                                                                                                                       >  2:211979/1‑149 (MQ=255)                                                                                                                                                                                                tttCCATTAAGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATgcg                                                                                                                       <  1:211979/149‑1 (MQ=255)                                                                                                                                                                                                         aGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAAc                       >  1:753393/1‑236 (MQ=255)                                                                                                                                                                                                         aGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAAc                       >  2:469195/1‑236 (MQ=255)                                                                                                                                                                                                         aGCGACTCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAAc                       >  1:242864/1‑236 (MQ=255)                                                                                                                                                                                                               tCAATGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGt                 <  2:34184/236‑1 (MQ=255)                                                                                                                                                                                                                 aaTGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTcc               <  1:442713/236‑1 (MQ=255)                                                                                                                                                                                                                 aaTGAAATGGAGATTTATCCAGGAATGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTc                <  2:563031/235‑1 (MQ=255)                                                                                                                                                                                                                  aTGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCg              <  2:737145/236‑1 (MQ=255)                                                                                                                                                                                                                   tGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAgg                                                                                                                                 >  2:158121/1‑120 (MQ=255)                                                                                                                                                                                                                   tGAAATGGAGATTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAgg                                                                                                                                 <  1:158121/120‑1 (MQ=255)                                                                                                                                                                                                                             aTTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGc                                                                                                                                                                                             <  2:496689/50‑1 (MQ=255)                                                                                                                                                                                                                             aTTTATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGc                                                                                                                                                                                             >  1:496689/1‑50 (MQ=255)                                                                                                                                                                                                                               ttATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTTTTGGCCTAGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATcccc  <  1:146110/235‑1 (MQ=255)                                                                                                                                                                                                                               ttATCCAGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATcccc  <  1:89306/235‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                     TTTACACTTTGGGCTGACAGGCACCTCTGCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCGCTGACGGTTATTGTTGGGTGATATAGCTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCC  >  NC_000913/2387077‑2387533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑