Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,395,394	A→C	L168R (CTG→CGG)	nuoJ ←	NADH:ubiquinone oxidoreductase, membrane subunit J

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,395,394	0	A	C	98.0%	160.7 / ‑3.2	49	L168R (CTG→CGG)	nuoJ	NADH:ubiquinone oxidoreductase, membrane subunit J
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (26/22);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

CATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACA  >  NC_000913/2395163‑2395622                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                     cATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCa                                                                                                                                                                                                                                   >  2:75876/1‑235 (MQ=255)          aCCGTTGGTCTGGCCACAGTAGCTTCCGTCGACCACGACGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGc                                                                                                                                                                                                                         <  2:406304/236‑1 (MQ=255)          aCCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGGCGTCTTTACGATTGCTCCGCACTTCACCCg                                                                                                                                                                                                                          >  1:349495/1‑235 (MQ=255)             gTCGCTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACg                                                                                                                                                                                                                      <  2:686180/236‑1 (MQ=255)                  tCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACAGAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACg                                                                                                                                                                                                                      <  2:160787/231‑1 (MQ=255)                      gCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCC‑TGTGGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTCGTCCGCACTTAACCCCAACGCTCTTCatg                                                                                                                                                                                                             >  2:113824/1‑234 (MQ=255)                             tAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACg                                                                                                                                                                                                      <  2:454210/236‑1 (MQ=255)                                 ttCCGGCGACCACGAAGGCCAGCGCGGATGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACg                                                                                                                                                                                                      <  1:438874/232‑1 (MQ=255)                                 ttCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTgg                                                                                                                                                                                                   <  1:219909/235‑1 (MQ=255)                                 ttCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGa                                                                                                                                                                                                  <  1:136859/236‑1 (MQ=255)                                 ttCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGa                                                                                                                                                                                                  <  1:180922/236‑1 (MQ=255)                                  tCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGa                                                                                                                                                                                                  >  2:746240/1‑235 (MQ=255)                                     ggCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGc                                                                                                                                                                                              <  1:210609/236‑1 (MQ=255)                                     ggCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGACCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGc                                                                                                                                                                                              >  1:759249/1‑236 (MQ=255)                                        gACCACTGAGGCCAACGCTGAAGCATTAATCATGATTTCCAGACCAATCAACGTAAACAGCAGATTGCGACGGATAACCAGGCCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCcac                                                                                                                                                                                           <  2:671016/236‑1 (MQ=255)                                                    cAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCCCAACCAGACCTg                                                                                                                                                                                >  2:181614/1‑235 (MQ=255)                                                       cgcgGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGa                                                                                                                                                                             >  1:89859/1‑235 (MQ=255)                                                           gaagCGTTAATCATTATTTCCAGACCAATCAACAAAAACAGCAGATTGCCACGGATAACCAGAACGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGag                                                                                                                                                                            <  2:349495/229‑1 (MQ=255)                                                           gAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGagcagc                                                                                                                                                                        <  1:746240/236‑1 (MQ=255)                                                           gAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGag                                                                                                                                                                            <  1:755573/232‑1 (MQ=255)                                                            aGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGACCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGagcagca                                                                                                                                                                       <  2:759249/236‑1 (MQ=255)                                                                  tAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATa                                                                                                                                                                  <  1:75876/235‑1 (MQ=255)                                                                        tGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGag                                                                                                                                                                            <  1:691599/219‑1 (MQ=255)                                                                        tGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGag                                                                                                                                                                            >  2:691599/1‑219 (MQ=255)                                                                                   ccAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGcc                                                                                                                                                >  1:158868/1‑236 (MQ=255)                                                                                           cATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGc                                                                                                                                                                                              >  1:712701/1‑182 (MQ=255)                                                                                           cATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGc                                                                                                                                                                                              <  2:712701/182‑1 (MQ=255)                                                                                                    cagATTGCGACGGATGACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGa                                                                                                                                >  1:542561/1‑235 (MQ=255)                                                                                                        tggcaaCGGAT‑ACCAGGCCGGTTTACGCAAAGACT‑GAATATGTTCCCCGC‑GAGTATCA‑TCCATTGTGGAAAGGGG‑ACCATGCGTGCCCCCCCTTTTTTTTTTGCGCGCTTTCGTTTTTACGTTTGCCCCGCCCTCCCCCCGCACGCTCTACACGCCCGACGTGGAGGCCCAAAACCAGCCCGGCGAGCAGCAGAATAGAACCCAGTTCCACCGCCAGTACGTAAGGCCCGAACa                                                                                                                             <  2:110594/229‑1 (MQ=255)                                                                                                            aaCGGATCA‑CAGGCCGG‑GTAAGCCAAGAACAGAAT‑AGTATCCCGCGGAGAATCAGTCC‑‑TGTTATGAGGGGAATCATGCGTCCTCTTCCGTTTTTCTTTTCGCGCTGTCGTTTTTACGATGCCCCCCCCCTCCCCCCCCCCGCTCTCCACGCCCGCCGGGGAAGCCCACACCCAGCCCGGGGGGAAGCAGAAAAGACGCCATTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTa                                                                                                                        <  2:542561/234‑1 (MQ=255)                                                                                                                 aTAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATa                                                                                                                     >  1:185450/1‑233 (MQ=255)                                                                                                                             g‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGc                                                                                                                                                                                              >  2:321574/1‑148 (MQ=255)                                                                                                                             g‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGc                                                                                                                                                                                              <  1:321574/148‑1 (MQ=255)                                                                                                                                        gAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCg                                                                                            >  1:561608/1‑236 (MQ=255)                                                                                                                                         aaC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGt                                                                                           >  1:513724/1‑236 (MQ=255)                                                                                                                                                                            gttgtAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGCCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGccc                                                                                                                                  >  1:516618/1‑165 (MQ=255)                                                                                                                                                                              tgtAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCTAGGTTg                                                         >  1:618218/1‑236 (MQ=255)                                                                                                                                                                                     gg‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGcc                                                                                                                                                                                             <  1:723630/97‑1 (MQ=255)                                                                                                                                                                                     gg‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGcc                                                                                                                                                                                             >  2:723630/1‑97 (MQ=255)                                                                                                                                                                                      g‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGCGGATTGctgaag                                                  >  2:628508/1‑230 (MQ=255)                                                                                                                                                                                        aTCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGt                                                                       >  1:339337/1‑213 (MQ=255)                                                                                                                                                                                          cATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCg                                                                                                                          >  1:315135/1‑160 (MQ=255)                                                                                                                                                                                                  ctcctcCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCAccacca                                      >  1:83805/1‑236 (MQ=255)                                                                                                                                                                                                  ctcctcCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCAccacca                                      >  1:210086/1‑236 (MQ=255)                                                                                                                                                                                                        cGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCatg                                 >  1:506745/1‑235 (MQ=255)                                                                                                                                                                                                             ttcttttcGCGCTGTCGTCTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGCTACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCagtaagtcc                           >  2:482541/1‑228 (MQ=255)                                                                                                                                                                                                                   tcGCGCTGTCGTCTTTACGATTCCCCCGCACTTCACCCGCCCGCTCTTCACGCCCGACGTGGAGGCCCACAACCAGCCCTGCGAGCAGCAGCATAGAAGCCAGTCCCACCGCCAGTACGTAAGGCCCGaa                                                                                                                               <  2:652415/130‑1 (MQ=255)                                                                                                                                                                                                                          gtcgtcTTTACGATTGCTCCGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTa                                                                                                                        >  1:652415/1‑130 (MQ=255)                                                                                                                                                                                                                                          cTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAAAACCGAGGATGGCGTAAACAATCACAACCAACGTGATGGCCGCCAAAAATGCCGGACCAATCcaca  >  2:722691/1‑232 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                     CATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGG‑TTAAGCCAAGAAC‑GAATAAGATTGCCGC‑GAGGATCAGTCCA‑TGTTGTAAGGGG‑ATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACA  >  NC_000913/2395163‑2395622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑