Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,421,142	T→G	T174T (ACT→ACG)	yfcG →	GSH‑dependent disulfide bond oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,421,142	0	T	G	97.8%	146.1 / ‑3.3	46	T174T (ACT→ACG)	yfcG	GSH‑dependent disulfide bond oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (23/21);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

GTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTC  >  NC_000913/2420920‑2421349                                                                                                                                                                                                                               |                                                                                                                                                                                                                gTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAAt                                                                                                                                                                                                      >  2:325664/1‑234 (MQ=255) gTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAAt                                                                                                                                                                                                      >  1:694596/1‑234 (MQ=255) gTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAAt                                                                                                                                                                                                      >  1:418674/1‑234 (MQ=255)    aTTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGCATTGAc                                                                                                                                                                                                  >  2:667678/1‑235 (MQ=255)         ggCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCa                                                                                                                                                                                            <  2:202377/236‑1 (MQ=255)         ggCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCa                                                                                                                                                                                            <  1:325664/236‑1 (MQ=255)          gCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCCTGGGTTAATGCCTGGACCCGCCAGCGAATTGACCTAGCaa                                                                                                                                                                                           >  1:117495/1‑236 (MQ=255)                          tggCCGATGCTTGGACAAAATCATCATTTTAATCACGCAGCCCCCAACACAATTCCTTATGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCgg                                                                                                                                                                                  <  2:418674/228‑1 (MQ=255)                              cGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGaa                                                                                                                                                                        <  2:131805/235‑1 (MQ=255)                                                             cGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGAcc                                                                                                                                                                                                 >  1:132911/1‑179 (MQ=255)                                                             cGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGAcc                                                                                                                                                                                                 <  2:132911/179‑1 (MQ=255)                                                                        aaaCCATTCCTTACGCGATTGAACGTTATCAGGTTAAAACTCAGCGTCGTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGggcaggca                                                                                                                             <  2:694596/236‑1 (MQ=255)                                                                                   tACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTTACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAACGGATCCGTTCGACCCCTTCCACCCGGCAGCGACGGCTAAAAGc                                                                                                                  >  2:350063/1‑236 (MQ=255)                                                                                                 ttttCAGGTTGAAACGCAGCGTCTGTACCATGTACGGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGtgatg                                                                                                    <  2:633704/233‑1 (MQ=255)                                                                                                  tATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACCGCTCAAACGACAATTCGGggatca                                                                                                   >  2:683668/1‑234 (MQ=255)                                                                                                          tGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCgg                                                                                           >  1:426645/1‑236 (MQ=255)                                                                                                              aCTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAg                                                                                       <  1:23539/236‑1 (MQ=255)                                                                                                                   gCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGACAGTTAAc                                                                                  >  1:60497/1‑236 (MQ=255)                                                                                                                          tACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACa                                                                           >  2:314905/1‑236 (MQ=255)                                                                                                                                 tACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTtctc                                                                    >  2:145943/1‑236 (MQ=255)                                                                                                                                  aCTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCg                                                                   >  2:47008/1‑236 (MQ=255)                                                                                                                                      aaCAAGCGGCTGGAAACCTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGta                                                               <  1:683668/236‑1 (MQ=255)                                                                                                                                           gtggCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACCGAAACAAGTTCTCGTGgattaat                                                          >  2:215024/3‑234 (MQ=255)                                                                                                                                           gCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGGGATGAGCGCTCGGATAGTTAACAGAAACAGGTTCTCGTGCattatt                                                          >  2:261245/1‑236 (MQ=255)                                                                                                                                           gCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTtctc                                                                    <  1:140452/226‑1 (MQ=255)                                                                                                                                           gCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTtctc                                                                    >  2:140452/1‑226 (MQ=255)                                                                                                                                           gCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTattatt                                                          >  1:738460/1‑236 (MQ=255)                                                                                                                                                         cGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTaaa                                            >  1:418302/1‑236 (MQ=255)                                                                                                                                                                gctggGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACgg                                     <  1:392999/236‑1 (MQ=255)                                                                                                                                                                   gggAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGCCCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGgaga                                  <  1:667678/236‑1 (MQ=255)                                                                                                                                                                           agaACTACAGCATTGCGGATTTTCCCTGCGGGCCGTGGGTTAAGGCCTGGACGCGCAAGCGAATTGGCCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCa                           <  2:426645/235‑1 (MQ=255)                                                                                                                                                                                 aCAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGca                     <  1:47008/235‑1 (MQ=255)                                                                                                                                                                                  cAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCt                                                   <  2:23370/204‑1 (MQ=255)                                                                                                                                                                                  cAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCt                                                   >  1:23370/1‑204 (MQ=255)                                                                                                                                                                                       ttGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTaaaa                                           <  2:40827/207‑1 (MQ=255)                                                                                                                                                                                       ttGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTaaaa                                           >  1:40827/1‑207 (MQ=255)                                                                                                                                                                                        ttcGGATTTAGCCTGTTAGCCGTGGATTAATGCCTGGACGCGCCAGCGAATTGCCCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTTTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGc             <  2:240806/234‑1 (MQ=255)                                                                                                                                                                                         gcgattatGGCCTACTGGCGGTGGGTTAATGCCTGGACGCGCCATGCAATTGACCTAGCAATGTATCCGGCGGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGc             <  2:60497/230‑1 (MQ=255)                                                                                                                                                                                         gCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAGCAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTg              >  2:536926/1‑234 (MQ=255)                                                                                                                                                                                         gCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGcc            <  1:145943/236‑1 (MQ=255)                                                                                                                                                                                          cGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGggcaggc                                                                                                                              >  2:563812/1‑121 (MQ=255)                                                                                                                                                                                          cGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGggcaggc                                                                                                                              <  1:563812/121‑1 (MQ=255)                                                                                                                                                                                               atTGCCTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGAACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGGTCTCGTGAATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATTGCACAACCTGCCGCaat       >  1:42809/1‑235 (MQ=255)                                                                                                                                                                                                   ccTGCTGGCCGTGGGTTAATGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTc  >  2:192075/1‑236 (MQ=255)                                                                                                                                                                                                                    aaTGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAAc                                                                                                             <  1:211020/112‑1 (MQ=255)                                                                                                                                                                                                                    aaTGCCTGGACGCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAAc                                                                                                             >  2:211020/1‑112 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                GTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTC  >  NC_000913/2420920‑2421349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑