Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,589,778 T→G D728E (GAT→GAG acrD → aminoglycoside/multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,589,7780TG100.0% 281.8 / NA 80D728E (GAT→GAGacrDaminoglycoside/multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (34/46);  total (34/46)

AGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGATATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCG  >  NC_000913/2589549‑2590003
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 
agccGTTTAACCAAATTAAAGAAGCTCGCTTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGAc                                                                                                                                                                                                                             <  2:194511/233‑1 (MQ=255)
aGGCGTTTACCAAAATGAAAGAAGCTCGCGTTAGCGCCAGCAGCCCGCCAGCAATTGGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGAc                                                                                                                                                                                                                             <  1:561585/236‑1 (MQ=255)
aGGCGTTTAACCAAATTAAAGATGCTCGCTTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGAc                                                                                                                                                                                                                             <  1:317277/236‑1 (MQ=255)
aGGCGTTTAACCAAATTAAAGAAGCTCGGGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGAc                                                                                                                                                                                                                             <  2:422186/236‑1 (MQ=255)
aGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGAc                                                                                                                                                                                                                             <  1:642222/236‑1 (MQ=255)
aGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGAc                                                                                                                                                                                                                             <  1:371807/236‑1 (MQ=255)
  ccGTTAAACCAAATGAAAGGAGTTCGCGTTATCGACAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTGGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGAc                                                                                                                                                                                                                             <  2:614390/233‑1 (MQ=255)
     tttAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCg                                                                                                                                                                                                                        >  1:424510/1‑236 (MQ=255)
         aCCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTaaa                                                                                                                                                                                                                    >  1:614390/1‑236 (MQ=255)
              aTTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGTCATAACGGCCTCGACGACAGTCCGCAGGTGCAGATTGAGATCGACCAGCGTAAAGCTCa                                                                                                                                                                                                               >  2:485983/1‑236 (MQ=255)
                  aagaagCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGgcg                                                                                                                                                                                                           <  1:567337/236‑1 (MQ=255)
                        cTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGc                                                                                                                                                                                                     >  2:163837/1‑236 (MQ=255)
                          cgcgTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGCCGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAgcggccgtgc                                                                                                                                                                                                     >  1:115986/1‑225 (MQ=255)
                                     cagcagCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATcgacg                                                                                                                                                                                        <  2:727553/236‑1 (MQ=255)
                                     cagcagCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTGGCGAtggtcg                                                                                                                                                                                        >  2:314943/1‑231 (MQ=255)
                                        cagcCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGCGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGata                                                                                                                                                                                     >  1:205409/1‑236 (MQ=255)
                                               ccAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGa                                                                                                                                                                              >  1:307412/1‑236 (MQ=255)
                                                 gactaTTAGCGTTCTTGGTAGTTATGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCGGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGaca                                                                                                                                                                            <  2:618244/232‑1 (MQ=255)
                                                 cagaaTTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGGATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGacac                                                                                                                                                                           <  2:345524/233‑1 (MQ=255)
                                                         gCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCaa                                                                                                                                                                    <  1:739762/236‑1 (MQ=255)
                                                                       tccgCAGTTTTTGATAGGGAGTTGCAGGTCCCAGCTGGGGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGtt                                                                                                                                                       <  2:481127/232‑1 (MQ=255)
                                                                       tCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGAc                                                                                                                                                                                                                             <  2:250615/165‑1 (MQ=255)
                                                                       tCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGAc                                                                                                                                                                                                                             >  1:250615/1‑165 (MQ=255)
                                                                       tCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGtt                                                                                                                                                       >  2:123060/1‑235 (MQ=255)
                                                                         tGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGat                                                                                                                                                                                      >  1:734546/1‑202 (MQ=255)
                                                                         tGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGat                                                                                                                                                                                      <  2:734546/202‑1 (MQ=255)
                                                                          gCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAg                                                                                                                                                   <  1:123060/236‑1 (MQ=255)
                                                                          gCAGGTTGTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAg                                                                                                                                                   <  1:473975/236‑1 (MQ=255)
                                                                            aGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCt                                                                                                                                                 <  1:163837/236‑1 (MQ=255)
                                                                                tttGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTc                                                                                                                                                      <  1:371334/227‑1 (MQ=255)
                                                                                tttGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTc                                                                                                                                                      >  2:371334/1‑227 (MQ=255)
                                                                                tttGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAAACGCCTGGGGTTCGAGCTAtgt                                                                                                                                             >  1:411249/1‑236 (MQ=255)
                                                                                 ttGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTAtgtg                                                                                                                                            >  1:557942/1‑236 (MQ=255)
                                                                                  tGATAGGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTAt                                                                                                                                               <  1:131675/232‑1 (MQ=255)
                                                                                   gATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGaa                                                                                                                                          >  2:208631/1‑236 (MQ=255)
                                                                                    atatGGAGTTGCAGGACCACGCTGGAGCGGGTAACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAAt                                                                                                                                         <  2:605726/236‑1 (MQ=255)
                                                                                        ggAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGGGAATGACt                                                                                                                                     >  2:307876/1‑236 (MQ=255)
                                                                                           gTTGCGGAACCGCGCGGGAGCGGTTCACGATGCGCTGATGGAAGCACGTAATCGGTTGCTGGCGCTGGCAGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTa                                                                                                                                  <  2:115986/236‑1 (MQ=255)
                                                                                              gCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATgg                                                                                                                               >  1:622999/1‑236 (MQ=255)
                                                                                                    ccACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAg                                                                                                                                                   <  1:530097/210‑1 (MQ=255)
                                                                                                    ccACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAg                                                                                                                                                   >  2:530097/1‑210 (MQ=255)
                                                                                                    ccACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACAGCCTGGGGTTCGAGCTATGGGAATGAATTTATGGATccag                                                                                                                         >  2:226638/1‑233 (MQ=255)
                                                                                                    ccACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAAAGACACACTGCAAACAGCCTGGGGTTCGAGCTATGTGTATGACTTTATGGactgcg                                                                                                                         >  2:469615/1‑231 (MQ=255)
                                                                                                      aCGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACCCTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGt                                                                                                                       >  2:583296/1‑236 (MQ=255)
                                                                                                      aCGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACAAACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGACcg                                                                                                                           >  1:616886/1‑232 (MQ=255)
                                                                                                       cGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACATTGCAAACCGCCTGGGGTTCGAGCCATGTGAAAGAAGTTATGGATCGCGGta                                                                                                                      >  2:37875/1‑235 (MQ=255)
                                                                                                                  gTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGaca                                                                                                                                                                            <  2:343844/171‑1 (MQ=255)
                                                                                                                  gTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGaca                                                                                                                                                                            >  1:343844/1‑171 (MQ=255)
                                                                                                                       gATGCGCTGATGGCAGGCAGTAAGCAGTGGCTGGCGCTGGCGGCGGAAAGCCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATAAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCt                                                                                                       <  2:605138/235‑1 (MQ=255)
                                                                                                                         tGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTAt                                                                                                     <  2:307412/235‑1 (MQ=255)
                                                                                                                            gcTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCCTTTGGAATGACTTATTGGAACGCGGTCCCGTGACGAAAGCCAAtgtta                                                                                                 >  2:552915/1‑234 (MQ=255)
                                                                                                                               gagggCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTgca                                                                                                <  1:740619/231‑1 (MQ=255)
                                                                                                                                aTGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGaa                                                                                                                                          >  1:482629/1‑191 (MQ=255)
                                                                                                                                aTGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGaa                                                                                                                                          <  2:482629/191‑1 (MQ=255)
                                                                                                                                  ggCAGCCCGTAAACCGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTgcaggcag                                                                                           <  2:186083/236‑1 (MQ=255)
                                                                                                                                     agcaCGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTCTGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGGCTATGTGCAGGCAGCt                                                                                         >  2:543231/1‑235 (MQ=255)
                                                                                                                                        aCGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTCTATGGATCGCGGTCCCGTGAAGAAAGTGTATGTGCAGGCGGCTgcgc                                                                                     >  2:214113/1‑236 (MQ=255)
                                                                                                                                          gTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGa                                                                                                                                           >  1:616662/1‑180 (MQ=255)
                                                                                                                                          gTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGa                                                                                                                                           <  2:616662/180‑1 (MQ=255)
                                                                                                                                           tAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTgcg                                                                                      <  2:622999/232‑1 (MQ=255)
                                                                                                                                                    gctggcgctggcGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTa                                                                                                                                  <  2:578098/179‑1 (MQ=255)
                                                                                                                                                    gctggcgctggcGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTa                                                                                                                                  >  1:578098/1‑179 (MQ=255)
                                                                                                                                                           ctggaggacaaaagCCAGGAGCACACCCGTGTGCGCCAGAAGGGCCACGACGAAATTCCGCAGTTGCGGATGGAGCTCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCAtgctgc                                                                      <  2:424510/222‑1 (MQ=255)
                                                                                                                                                            ttgggacggAAACCGCGGAGCAAGCCCGTGTGGGCCGTAACGGCCGCGACGACAGTACGCATGTGGAGGTTGAGATCGACCAGCGTAAAGCTCATGCGCTGGGCGTTGCTATCGACGATATTAACGACACAGTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGa                                                                  <  2:428785/229‑1 (MQ=255)
                                                                                                                                                            tggcgGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGa                                                                  <  1:624903/235‑1 (MQ=255)
                                                                                                                                                                   ggAAAACCCGGAGCTAACCCGTGTGCGCCATACCGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTTCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATc                                                           <  1:226638/235‑1 (MQ=255)
                                                                                                                                                                   ggAAAACCCGGAGCTAACCCGTGGTCGGCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATc                                                           <  1:552915/235‑1 (MQ=255)
                                                                                                                                                                        aCCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAAtct                                                      <  2:302622/235‑1 (MQ=255)
                                                                                                                                                                                        tgtgCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATcgacg                                                                                                                                                                                        <  2:463956/89‑1 (MQ=255)
                                                                                                                                                                                        tgtgCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATcgacg                                                                                                                                                                                        >  1:463956/1‑89 (MQ=255)
                                                                                                                                                                                          tgCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCa                                                          >  2:453711/1‑213 (MQ=255)
                                                                                                                                                                                          tgCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCa                                                          >  2:723200/1‑213 (MQ=255)
                                                                                                                                                                                          tgCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCa                                                          <  1:723200/213‑1 (MQ=255)
                                                                                                                                                                                          tgCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCa                                                          <  1:453711/213‑1 (MQ=255)
                                                                                                                                                                                                 tagacgCCTCGGCGACAGTCCGCAGTTACAGAGTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATggcg                            <  2:313107/231‑1 (MQ=255)
                                                                                                                                                                                                  aaCGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAg                                                                                                                                                   <  1:519063/116‑1 (MQ=255)
                                                                                                                                                                                                  aaCGGCCTCGACGACAGTCCGCAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAg                                                                                                                                                   >  2:519063/1‑116 (MQ=255)
                                                                                                                                                                                                    cGGCCTGGGCGACAGTCCGGAGTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCa                         <  2:557942/236‑1 (MQ=255)
                                                                                                                                                                                                                         gTTGCAGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACTCACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTcg    <  1:469615/236‑1 (MQ=255)
                                                                                                                                                                                                                              aGATTGAGATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTcgcg  <  1:307876/233‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 
AGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGATGCGCTGATGGCAGCACGTAATCAGTTGCTGGCGCTGGCGGCGGAAAACCCGGAGCTAACCCGTGTGCGCCATAACGGCCTCGACGACAGTCCGCAGTTGCAGATTGATATCGACCAGCGTAAAGCTCAGGCGCTGGGCGTTGCTATCGACGATATTAACGACACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCG  >  NC_000913/2589549‑2590003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: