Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,668,449	A→C	Y150D (TAT→GAT)	hcaR ←	hca operon transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,668,449	0	A	C	100.0%	214.3 / NA	59	Y150D (TAT→GAT)	hcaR	hca operon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/31);  total (28/31)

CACCAGAATATTCGTTGCCACCTGGACGATATTTGGCTGGCTGTTTTCTTGCGCAAACCACGCTTTAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATAAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGCACAAAGCCAATGGTTAATTGTCTGTCTTCCTGAACAATTTTCCGCGCC  >  NC_000913/2668216‑2668654                                                                                                                                                                                                                                          |                                                                                                                                                                                                              cACCAGAATATTCGTTGCCACCTGAACGATTTTGGGCTGGCTGTTTTCTTGCGCAAACCACGCTTTAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATcaa                                                                                                                                                                                                             <  2:116009/236‑1 (MQ=255)  aCCAGAATATTCGTTGCCACCTGGACGATATTTGGCTGGCTGTTTTCTTGCGCAAACCACGCTTTAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAAc                                                                                                                                                                                                            >  1:606604/1‑236 (MQ=255)  aCCAGAATATTCGTTGCCACCTGGACGATATTTGGCTGGCTGTTTTCTTGCGCAAACCACGCTTTAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGATCCAGATAATCAAAATCCGGGCTATCAAc                                                                                                                                                                                                            >  2:341414/1‑236 (MQ=255)              gTTGCCACCTGGACGATATTTGGCTGGCTGTTTTCTTGCGCAAACCACGCTTTAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAAACACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATTCCCGGGCCATCAAAAGGATGGCGCAt                                                                                                                                                                                                >  2:659105/1‑236 (MQ=255)                tGCCACCTAGACCATATTTGGCCTGCTGTTTTCTTGCGCAAACCACGCTTTAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTa                                                                                                                                                                                              <  2:454529/236‑1 (MQ=255)                          aCGATATTTGGCTGGCTGGTTTCTTGCGCAAACCACGCTTTAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTc                                                                                                                                                                                    <  1:606132/236‑1 (MQ=255)                              tatTTGGCTGGCTGTTTTCTTGCGCAAACCACGCTTTAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAg                                                                                                                                                                                 >  1:403611/1‑235 (MQ=255)                               atTTGGCTGGCTGTTTTCTTGCGCAAACCACGCTTTAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAgg                                                                                                                                                                                                         <  2:489536/210‑1 (MQ=255)                               atTTGGCTGGCTGTTTTCTTGCGCAAACCACGCTTTAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAgg                                                                                                                                                                                                         >  1:489536/1‑210 (MQ=255)                                   ggctggctGTTTTCTTGCGCAAACCACGCTTTAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTcacc                                                                                                                                                                           >  1:191737/1‑236 (MQ=255)                                                              tcTTAAACGATCGGCACAAGCGAACCGGAATACACCGGATCGGTACTTACGAAATTCACGCCATCCAGTTGCGCGGCTGTTATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCgt                                                                                                                                                <  2:25053/235‑1 (MQ=255)                                                              gCTTTAACGATCGGCGCAAGCGAACCGGAATACCCCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCgt                                                                                                                                                <  2:36638/236‑1 (MQ=255)                                                              gCTTTAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCgt                                                                                                                                                <  1:341414/236‑1 (MQ=255)                                                                tttAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCgtgg                                                                                                                                              <  1:517769/236‑1 (MQ=255)                                                                   aaCGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCCATCAACAGGATGGCGCATTAACACGACGTCGAGTTCACCCCGGCGAATATTTTCCTCCTGTTGCGTGCTGa                                                                                                                                           >  2:225939/1‑236 (MQ=255)                                                                          ggCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAAc                                                                                                                                     >  1:495483/1‑235 (MQ=255)                                                                            ccaaaccGAACCGGAATCCCCCGGGTCGGTACTGGCGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCa                                                                                                                                  <  2:212924/230‑1 (MQ=255)                                                                                gtcGAACCGGAATACACCGTATCGGTACTGACGAACTGCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCAGGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGATATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAg                                                                                                                              <  1:360109/234‑1 (MQ=255)                                                                                aGCGAACCGGAATACCCCGGATTGGTACTGACGAAATTCACGGCATCCAATTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAg                                                                                                                              <  2:191737/236‑1 (MQ=255)                                                                                                    aTCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGgtgt                                                                                                                >  2:632529/1‑230 (MQ=255)                                                                                                        gTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGCTCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTc                                                                                                       <  2:208193/235‑1 (MQ=255)                                                                                                         tACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCt                                                                                                                            <  2:438653/213‑1 (MQ=255)                                                                                                         tACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCt                                                                                                                            >  1:438653/1‑213 (MQ=255)                                                                                                         tACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTg                                                                                                     <  1:632529/236‑1 (MQ=255)                                                                                                              aCGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAg                                                                                                                                                                                 <  1:739117/155‑1 (MQ=255)                                                                                                              aCGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAg                                                                                                                                                                                 >  2:739117/1‑155 (MQ=255)                                                                                                              aCGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCgtg                                                                                                                                               <  1:73272/189‑1 (MQ=255)                                                                                                              aCGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCgtg                                                                                                                                               >  2:73272/1‑189 (MQ=255)                                                                                                              aCGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACg                                                                                                >  1:572401/1‑236 (MQ=255)                                                                                                                  aaTTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAAc                                                                                            <  1:659105/236‑1 (MQ=255)                                                                                                                  aaTTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAAc                                                                                            <  2:403611/236‑1 (MQ=255)                                                                                                                   aTTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACa                                                                                           >  1:394829/1‑236 (MQ=255)                                                                                                                   aTTCACGCCATCCAATTGCGCGGCGTTGATCTCTTTTTCATGTGCTAAAGGGTTATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGAAAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAAc                                                                                            <  2:610605/235‑1 (MQ=255)                                                                                                                    ttCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACAt                                                                                          <  2:394829/236‑1 (MQ=255)                                                                                                                     tcaccgcATCCAGTTGCCGGGCGGTGATCTCGTTTTCATGTGCTAAAGGGTAATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACAt                                                                                          <  2:734861/229‑1 (MQ=255)                                                                                                                        cGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATTCAGGTGTATGGCTGTCTGAGACGTAACATCGGt                                                                                      >  1:610605/1‑236 (MQ=255)                                                                                                                                     cgcgGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCa                                                                         >  2:643236/1‑236 (MQ=255)                                                                                                                                      gcggcgGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGc                                                                          <  1:451403/234‑1 (MQ=255)                                                                                                                                      gcggccGTTATCTCTTTTTCATGTGCTAAAGGGTAATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTg                                                                            <  2:606604/232‑1 (MQ=255)                                                                                                                                       cggcggTGATCTCTTGTTCATGTGCTAAAGGGTGATCAACTGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCCCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGt                                                                       <  1:525939/236‑1 (MQ=255)                                                                                                                                                         cATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGAt                                                     >  1:242366/1‑236 (MQ=255)                                                                                                                                                           tgtgCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGTCAGTCAATTCACTTCCGACGATgg                                                   >  2:758440/1‑236 (MQ=255)                                                                                                                                                           tgtgCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCa                                                                         <  2:288220/214‑1 (MQ=255)                                                                                                                                                           tgtgCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCa                                                                         >  1:288220/1‑214 (MQ=255)                                                                                                                                                              gCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGca                                                 >  1:158890/1‑235 (MQ=255)                                                                                                                                                              gCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGca                                                 >  2:606670/1‑235 (MQ=255)                                                                                                                                                               cTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTGCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGcaca                                               <  1:437457/236‑1 (MQ=255)                                                                                                                                                                   aggtgaaTCAACCGGTAACACACACATAAATGGTTCGTCAAAAAGCTCAAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGCACAAAGc                                           <  2:412412/230‑1 (MQ=255)                                                                                                                                                                       tGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGCACAAAGCCAAt                                       >  2:99959/1‑236 (MQ=255)                                                                                                                                                                         aTCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAg                                                                                                                              >  2:441952/1‑147 (MQ=255)                                                                                                                                                                         aTCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAg                                                                                                                              <  1:441952/147‑1 (MQ=255)                                                                                                                                                                         aTCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGCACAAAGCCAATgg                                     >  1:412412/1‑236 (MQ=255)                                                                                                                                                                         aTCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGGGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGCACAAAGCCAATgg                                     >  1:437540/1‑236 (MQ=255)                                                                                                                                                                          tCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGCACAAAGCCAATGGt                                    >  2:593000/1‑236 (MQ=255)                                                                                                                                                                               cGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGCACAAAGCCAATGGTTAAtt                               <  1:643236/236‑1 (MQ=255)                                                                                                                                                                               cGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGCACAAAGCCAATGGTTAAtt                               <  1:593000/236‑1 (MQ=255)                                                                                                                                                                                                     tCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGCACAAAGCCAATGGTTAATTGTCTGTCTTCCTGAACAAttt          >  1:243415/1‑235 (MQ=255)                                                                                                                                                                                                      cGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGCACAAAGCCAATGGTTAATTGTCTGTCTTCCTGAACAAtttt         >  1:189062/1‑235 (MQ=255)                                                                                                                                                                                                        gcaaaaccttCCAGATAATCACTCGCCGGGCTATCAACAGGATGGCGAATTAAGCCGACGTCGAGTTCCCCACGGCGAATTTTTTCCGCCGGTTGCGTGGTGATTAAACTCCCCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGCACAAAGCCAATGGTTAATTGTCTGTCTTCCTGAACAATTTTCcg      <  2:158890/227‑1 (MQ=255)                                                                                                                                                                                                            aaaGCTCCAGATAATCAATCTCCGGGCTATCAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGCACAAAGCCAATGGTTAATTGTCTGTCTTCCTGAACAATTTTCCGCGcc  <  2:495483/236‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                              CACCAGAATATTCGTTGCCACCTGGACGATATTTGGCTGGCTGTTTTCTTGCGCAAACCACGCTTTAACGATCGGCGCAAGCGAACCGGAATACACCGGATCGGTACTGACGAAATTCACGCCATCCAGTTGCGCGGCGGTGATCTCTTTTTCATGTGCTAAAGGGTGATCAACCGGTAACACAACCACTAATGGTTCGTCAAAAAGCTCCAGATAATCAATCTCCGGGCTATAAACAGGATGGCGCATTAAGCCGACGTCGAGTTCACCACGGCGAATTTTTTCCTCCTGTTGCGTGGTGATTAAACTCACCAGCTCAATCAAGGTGTCTGGCTGTCTGAGACGAAACATCGGTAATACTTTTGGCAGTAAATTCACTTCCGCCGATGGCACAAAGCCAATGGTTAATTGTCTGTCTTCCTGAACAATTTTCCGCGCC  >  NC_000913/2668216‑2668654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑