Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,769,390 A→C R242R (CGA→CGC yfjQ → CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,769,3900AC94.1% 146.6 / 1.3 52R242R (CGA→CGCyfjQCP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base C (20/28);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

GAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCG  >  NC_000913/2769162‑2769624
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          
gcggtatgccatgccGTCGTTGCTGTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACt                                                                                                                                                                                                                                         <  2:474107/227‑1 (MQ=255)
gagGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCa                                                                                                                                                                                                                                       <  1:264112/234‑1 (MQ=255)
                  gTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGCAAACCTGCTTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGCCGGACCCACTCAAACTCGTGCCGTGCCTGGCa                                                                                                                                                                                                                   >  1:89396/1‑236 (MQ=255)
                       ttttACCGACTCCGGGGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTGGGTGAGGGCCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGa                                                                                                                                                                                                               <  2:275265/235‑1 (MQ=255)
                          tACCGACAGCTGTGCAGCAGGCACTGGAAGAAGCAGCGCTTAGGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACgg                                                                                                                                                                                                            <  2:647045/235‑1 (MQ=255)
                              gCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGAtg                                                                                                                                                                                                       <  1:5421/236‑1 (MQ=255)
                                cacaTGTGCAGCAGGCACGGGCAAACGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCATCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGAtgtg                                                                                                                                                                                                     <  2:556505/236‑1 (MQ=255)
                                cACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGAtgt                                                                                                                                                                                                      >  1:426358/1‑235 (MQ=255)
                                cACCTGTGCAGCAGCCCCTGGCAAAAGCAGCGCTTCCGTATCGCTTGAGTGAGGACCCCCAGCCGGTTACTGAATCGCAGATACTCCCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGAtgtg                                                                                                                                                                                                     <  2:274517/236‑1 (MQ=21)
                                     gtgCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCt                                                                                                                                                                                                <  1:61703/236‑1 (MQ=255)
                                             ggCACTGGCAAAAGCCGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAACCCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTg                                                                                                                                                                                        <  1:495474/236‑1 (MQ=255)
                                                aCTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTgcgc                                                                                                                                                                                     >  2:249913/1‑236 (MQ=255)
                                                aCTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGAGGTGAAGCTTAACCg                                                                                                                                                                                          >  1:71482/1‑231 (MQ=255)
                                                  tGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTgcg                                                                                                                                                                                      <  1:406739/233‑1 (MQ=255)
                                                         agcgacgcTTGCGTAGCGCTTTGGTCAGGACCCCCAGGCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTg                                                                                                                                                                             <  2:55908/230‑1 (MQ=255)
                                                          gcagcGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACGCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGAt                                                                                                                                                                           <  2:690227/236‑1 (MQ=255)
                                                                    cGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAaca                                                                                                                                                                 <  1:390684/236‑1 (MQ=255)
                                                                                 tGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAAc                                                                                                                                                    <  1:249913/236‑1 (MQ=255)
                                                                                   aGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATAGCCGAGACACTGCTCACGCAACtc                                                                                                                                                  >  2:753626/1‑235 (MQ=255)
                                                                                               cGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGttt                                                                                                                                      >  1:739411/1‑236 (MQ=255)
                                                                                                  ttagtaaaTCTCAGATACTCTCCCCACGACGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTAACGCAACTGCAGTAGACGtt                                                                                                                                       <  2:706539/226‑1 (MQ=255)
                                                                                                  ttACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCAt                                                                                                                                   >  1:394291/1‑236 (MQ=255)
                                                                                                          tcgaggATTCTATCCCCACGCCGCTGGCAGGATGAGGGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCAc                                                                                                                           <  2:71482/231‑1 (MQ=255)
                                                                                                            gCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCCGTAGACGTGTCATGTTGCCACgt                                                                                                                         >  1:287436/1‑236 (MQ=255)
                                                                                                             cAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACgtt                                                                                                                        <  2:739411/236‑1 (MQ=255)
                                                                                                                      tcCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                                                             >  1:34280/1‑222 (MQ=255)
                                                                                                                      tcCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                                                             <  2:34280/222‑1 (MQ=255)
                                                                                                                              ccgcTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGaca                                                                                                          >  2:277087/1‑233 (MQ=255)
                                                                                                                                gcTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGaca                                                                                                          >  2:403536/1‑231 (MQ=255)
                                                                                                                                  tGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGaca                                                                                                          >  1:134638/1‑229 (MQ=255)
                                                                                                                                     cAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTcc                                                                                                <  2:394291/236‑1 (MQ=255)
                                                                                                                                             gagCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCt                                                                                        <  1:403536/236‑1 (MQ=255)
                                                                                                                                                          tGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCa                                                                                                                                                                       <  1:528807/144‑1 (MQ=255)
                                                                                                                                                          tGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCa                                                                                                                                                                       >  2:528807/1‑144 (MQ=255)
                                                                                                                                                                      cAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACATTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTGGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAAGccccc                                                               >  1:315930/1‑236 (MQ=255)
                                                                                                                                                                           gATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCgtg                                                                             <  2:492286/217‑1 (MQ=255)
                                                                                                                                                                           gATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCgtg                                                                             >  1:492286/1‑217 (MQ=255)
                                                                                                                                                                            aTTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCg                                                                                                              <  2:566183/183‑1 (MQ=255)
                                                                                                                                                                            aTTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCg                                                                                                              >  1:566183/1‑183 (MQ=255)
                                                                                                                                                                            aTTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATcccc                                                                <  2:585194/229‑1 (MQ=255)
                                                                                                                                                                            aTTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATcccc                                                                >  1:585194/1‑229 (MQ=255)
                                                                                                                                                                             ttCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTtat                                                         <  2:134638/235‑1 (MQ=255)
                                                                                                                                                                                       ccggATTAAGGGCGGACTCAGTGGGCGTAATGCCAAAGGCTGACGCACTCATTCTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAcc                                              <  2:89396/233‑1 (MQ=255)
                                                                                                                                                                                                     gACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATccc                                                                 <  2:296964/203‑1 (MQ=255)
                                                                                                                                                                                                     gACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATccc                                                                 >  1:296964/1‑203 (MQ=255)
                                                                                                                                                                                                     gACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGtt                                        >  1:664420/1‑228 (MQ=255)
                                                                                                                                                                                                     gACTCAGTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGtt                                        >  1:373718/1‑228 (MQ=255)
                                                                                                                                                                                                           gTGGGCGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTg                          <  1:277087/236‑1 (MQ=255)
                                                                                                                                                                                                                cGTAATGCTAAAGGCGGACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGac                     >  1:116648/1‑235 (MQ=255)
                                                                                                                                                                                                                 gTAATGCTAAAGGCGGACGGACTCATACTCGTTCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTTCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGaa                     <  1:500221/235‑1 (MQ=255)
                                                                                                                                                                                                                               ggACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtgtg                                                                                   >  2:597816/1‑159 (MQ=255)
                                                                                                                                                                                                                               ggACGCACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtgtg                                                                                   <  1:597816/159‑1 (MQ=255)
                                                                                                                                                                                                                                    aaCTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCg  <  1:753626/235‑1 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          
GAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCG  >  NC_000913/2769162‑2769624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: