Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,044,628	A→C	K323Q (AAG→CAG)	bglA →	6‑phospho‑beta‑glucosidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,044,628	0	A	C	100.0%	248.7 / NA	74	K323Q (AAG→CAG)	bglA	6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/38);  total (36/38)

ACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAACGACGACTACCG  >  NC_000913/3044410‑3044851                                                                                                                                                                                                                           |                                                                                                                                                                                                                                aCTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCAcc                                                                                                                                                                                                                >  1:185895/1‑236 (MQ=255)      tGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGGGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTAAGCTATTCCATGCCCAATGCAGTGCGGCCCGACGGCGGAACCg                                                                                                                                                                                                               >  2:159967/1‑232 (MQ=255)        tAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatg                                                                                                                                                                                                         >  2:496430/1‑236 (MQ=255)             cgtgcGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTAACTTGGTTTCAGCTATTACATGACCAATGCATTGCAGGCCGACGGCGGCACCGtcgctgggctc                                                                                                                                                                                                    >  2:569674/5‑231 (MQ=255)               gACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACAGgc                                                                                                                                                                                                             >  2:578/1‑225 (MQ=255)                            tCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTCCAGGCCGAAGTCGGCACCGTGCAGTCGTTCTCCGGTTTTGAAgga                                                                                                                                                                                     >  2:457829/1‑235 (MQ=255)                              gCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGGTTTGAAGGCa                                                                                                                                                                                    >  1:144526/1‑235 (MQ=255)                                tCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGc                                                                                                                                                                                  >  1:153534/1‑235 (MQ=255)                                   ggAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTa                                                                                                                                                                               <  2:328175/235‑1 (MQ=255)                                     aGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTAAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGAAGCGAACTCTGGCTTTGAAGTCAGCGTAcc                                                                                                                                                                             >  2:131849/1‑235 (MQ=255)                                       tCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGACGGCGGCACCGGCGATGCGATCTCTGGGTTTGAAGGCAGCGTACCaaa                                                                                                                                                                          >  1:456310/1‑236 (MQ=255)                                        cGATGCGCGAACGCTACGTCTTTGCCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCa                                                                                                                                                                            <  1:329897/233‑1 (MQ=255)                                           tGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAAccc                                                                                                                                                                       <  2:185895/235‑1 (MQ=255)                                           tGCGCGAACGCTACGTCTGTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCg                                                                                                                                                                      <  1:737583/236‑1 (MQ=255)                                            gtgcgAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATACAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAAc                                                                                                                                                                         >  2:368489/3‑232 (MQ=255)                                              gcgAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCa                                                                                                                                                                                                                  >  2:668783/1‑189 (MQ=255)                                              gcgAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCa                                                                                                                                                                                                                  <  1:668783/189‑1 (MQ=255)                                               cgAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTg                                                                                                                                                                                                >  1:367689/1‑206 (MQ=255)                                               cgAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTg                                                                                                                                                                                                <  2:367689/206‑1 (MQ=255)                                                                     gtgCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAg                                                                                                                                                                                   >  2:554488/1‑197 (MQ=255)                                                                     gtgCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAg                                                                                                                                                                                   <  1:554488/197‑1 (MQ=255)                                                                              cgcgGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTTGGGCTGGCAGATTGa                                                                                                                                   >  1:395614/1‑236 (MQ=255)                                                                                    tATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAg                                                                                                                              <  1:614619/235‑1 (MQ=255)                                                                                        aCCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGt                                                                                                                         <  2:153534/236‑1 (MQ=255)                                                                                             tCCTATGTGTTGAACGAGTGGGAGCGTCGCGGCTTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGGGAAGGCACTTGCGCTTATCTTGGCTTCAGCTATTACATGACCAATGAAGCGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGTCTGCGTACCAAACTCGGATGTTTAAGAAACAGACGTGGGCTGGACGATTGATCCAGTCGGGCTtcg                                                                                                                    >  2:29110/1‑236 (MQ=255)                                                                                             tCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTg                                                                                                                                                                                                >  2:308024/1‑160 (MQ=255)                                                                                             tCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTg                                                                                                                                                                                                <  1:308024/160‑1 (MQ=255)                                                                                             tCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGt                                                                                                                         <  2:589976/231‑1 (MQ=255)                                                                                             tCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGt                                                                                                                         >  1:589976/1‑231 (MQ=255)                                                                                               cTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCt                                                                                                                  <  1:368489/236‑1 (MQ=255)                                                                                                  tgtgTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAgg                                                                                                                                                                                                                       <  2:331989/132‑1 (MQ=255)                                                                                                  tgtgTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAgg                                                                                                                                                                                                                       >  1:331989/1‑132 (MQ=255)                                                                                                  tgtgTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTa                                                                                                                 >  2:170151/1‑234 (MQ=255)                                                                                                       tGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAACCCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACtt                                                                                                          >  2:734615/1‑236 (MQ=255)                                                                                                       tGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACtt                                                                                                          >  2:221499/1‑236 (MQ=255)                                                                                                       tGAACGAGGGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGTGCTATgacctt                                                                                                          >  2:112910/1‑231 (MQ=255)                                                                                                           cGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCg                                                                                                      <  1:170151/236‑1 (MQ=255)                                                                                                            gAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGGTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCGAGTAGGTCCGCGCTATGCACCTTGcta                                                                                                     >  2:347497/1‑234 (MQ=255)                                                                                                             aGTGGGAACGTCGCGAATTGAACATCAAAATGGAAGGCGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGaa                                                                                                    <  2:144526/236‑1 (MQ=255)                                                                                                              gTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGaa                                                                                                    <  1:140912/235‑1 (MQ=255)                                                                                                               tGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAg                                                                                                                                                                                   <  2:145044/155‑1 (MQ=255)                                                                                                               tGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAg                                                                                                                                                                                   >  1:145044/1‑155 (MQ=255)                                                                                                                     cGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATg                                                                                             <  1:569674/235‑1 (MQ=255)                                                                                                                                 aaCATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCaga                                                                                 <  2:297770/235‑1 (MQ=255)                                                                                                                                    aTCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTT‑AAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCg                                                                            <  1:457829/236‑1 (MQ=255)                                                                                                                                     tCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTAGCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCAATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCg                                                                            <  1:29110/236‑1 (MQ=255)                                                                                                                                                gACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGt                                                                 >  2:36431/1‑236 (MQ=255)                                                                                                                                                      gATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGaaaa                                                           <  1:500875/236‑1 (MQ=255)                                                                                                                                                            gATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCAc                                                                                                            <  1:309158/181‑1 (MQ=255)                                                                                                                                                            gATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCAc                                                                                                            >  2:309158/1‑181 (MQ=255)                                                                                                                                                                tgctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTgg                                                  <  1:221499/235‑1 (MQ=255)                                                                                                                                                                  ctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGgcg                                                >  1:16154/1‑235 (MQ=255)                                                                                                                                                                  ctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGCGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGgcg                                                >  2:709689/1‑235 (MQ=255)                                                                                                                                                                   tgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCt                                              >  1:463078/1‑236 (MQ=255)                                                                                                                                                                    gagtaaaGGCACCTGCGATTATCGTGGTTTCAGCTTTTACATGTCCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCt                                              <  2:80269/230‑1 (MQ=255)                                                                                                                                                                      gTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTAc                                           >  1:557197/1‑236 (MQ=255)                                                                                                                                                                           ggCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGTTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACaa                                      <  1:578/236‑1 (MQ=255)                                                                                                                                                                           ggCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACa                                       <  1:496430/235‑1 (MQ=255)                                                                                                                                                                               ccTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGTGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTTGATGAGCGTTATCAAAGGCCGCTGTTTATTGTTGACAAAGGTTTTGGCGCTTCCGACaaaagg                                  >  2:695859/1‑233 (MQ=255)                                                                                                                                                                               ccTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTg                                  >  1:35566/1‑236 (MQ=255)                                                                                                                                                                                          cTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATgg                       <  1:131849/236‑1 (MQ=255)                                                                                                                                                                                           ttGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGgc                      <  1:734615/236‑1 (MQ=255)                                                                                                                                                                                                ttCAGCTATTACATGACCAATGCAGGGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCAt                 <  1:709689/236‑1 (MQ=255)                                                                                                                                                                                                    gCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAac             <  1:36431/236‑1 (MQ=255)                                                                                                                                                                                                         tacaggccaaaTGCAGAGCAGGCCGAAGGAGGCACCGGCGATGCAATCTCTGGTTTTGAAGGCAGCGTACCAAACCAGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAAcgacga        <  2:725621/227‑1 (MQ=255)                                                                                                                                                                                                           cattgccAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCGCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCGCTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAACGACGACt      <  1:610369/231‑1 (MQ=255)                                                                                                                                                                                                            aTGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAACGACGACTa     <  1:112910/236‑1 (MQ=255)                                                                                                                                                                                                             tGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGa                                                                                                                                   >  1:64109/1‑109 (MQ=255)                                                                                                                                                                                                             tGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGa                                                                                                                                   <  2:64109/109‑1 (MQ=255)                                                                                                                                                                                                               aCCAAGGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCCGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAACGACGACTACCg  <  1:347497/236‑1 (MQ=255)                                                                                                                                                                                                                    tgcagtgcagGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTAt                                                                    <  2:327930/165‑1 (MQ=255)                                                                                                                                                                                                                    tgcagtgcagGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTAt                                                                    >  1:327930/1‑165 (MQ=255)                                                                                                                                                                                                                     gCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACaa                                      >  1:67160/1‑194 (MQ=255)                                                                                                                                                                                                                     gCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACaa                                      <  2:67160/194‑1 (MQ=255)                                                                                                                                                                                                                        gTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTTCCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACa                                       <  1:478701/190‑1 (MQ=255)                                                                                                                                                                                                                        gTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACa                                       >  2:478701/1‑190 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                ACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAACGACGACTACCG  >  NC_000913/3044410‑3044851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑