Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,052,652 T→G Q289H (CAA→CAC ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,052,6520TG96.8% 197.2 / ‑1.9 62Q289H (CAA→CACubiH2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (28/32);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

GTTGCGCCCGACAACCAGCGGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGC  >  NC_000913/3052421‑3052880
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      
gTTGCGCCCGACAACCAGCGGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTgcg                                                                                                                                                                                                                                    <  1:385360/236‑1 (MQ=255)
   gcgcCTGACAACCAGCGGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGcgcc                                                                                                                                                                                                                                  <  2:494498/235‑1 (MQ=255)
    cgcCCGACAACCAGCGGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCTGGTGCAGAGTGTGagccgc                                                                                                                                                                                                                                >  2:271615/1‑236 (MQ=255)
    cgcCCGACAACCAGCGGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGcgccg                                                                                                                                                                                                                                 >  1:479328/1‑235 (MQ=255)
      cccGACAACCAGCAGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGCTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCa                                                                                                                                                                                                                               <  2:429466/235‑1 (MQ=255)
            aaCCAGCGGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCCCATCTCGCATCCCTAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGGTGGCCGCATTGccc                                                                                                                                                                                                                         >  2:155938/1‑235 (MQ=255)
             aCCAGCGGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCccac                                                                                                                                                                                                                       <  1:254081/236‑1 (MQ=255)
               cAGCGGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCccacca                                                                                                                                                                                                                     <  1:284333/236‑1 (MQ=255)
                    gTGACCAACGGTGGTCAAAACGTTGTAAAAGGACGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATAAGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATAACATCTCGCATACCGAGGTTAAAACCTTGAAAGGAAAGAGGGGGAAGAGGGGGCGCCGCAGTGCCCACCAGAACg                                                                                                                                                                                                                <  1:715615/236‑1 (MQ=255)
                       cccAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTa                                                                                                                                                                                                             >  1:561811/1‑236 (MQ=255)
                        ccAACGGTTGGCAAAAAGTTGTTACAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTa                                                                                                                                                                                                             <  2:300358/235‑1 (MQ=255)
                          aaCGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACg                                                                                                                                                                                                           <  1:696733/235‑1 (MQ=255)
                           atggTTCGCAAAAGGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGAt                                                                                                                                                                                                         <  2:311057/234‑1 (MQ=255)
                           agggTTGGCAAAAATATCGACAAGTCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGa                                                                                                                                                                                                          <  2:503416/233‑1 (MQ=255)
                             ggTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATgg                                                                                                                                                                                                       <  1:83290/236‑1 (MQ=255)
                                 ggCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTaa                                                                                                                                                                                                   >  2:293544/1‑236 (MQ=255)
                                          aTGTACACGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATACCGGCACAATCCGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTgg                                                                                                                                                                                          <  1:285838/236‑1 (MQ=255)
                                                       tCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGAt                                                                                                                                                                                                         >  2:318051/1‑208 (MQ=255)
                                                       tCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGAt                                                                                                                                                                                                         <  1:318051/208‑1 (MQ=255)
                                                       tCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTa                                                                                                                                                                             >  2:63391/1‑235 (MQ=255)
                                                       tCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTa                                                                                                                                                                             >  2:735731/1‑235 (MQ=255)
                                                            gACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCTGGCGttaacccacgcc                                                                                                                                                                        <  1:395104/236‑12 (MQ=255)
                                                             accccAATGGTGCCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCATCCATGTCTTCTCCGCGCCCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAg                                                                                                                                                                      <  2:431147/233‑1 (MQ=255)
                                                                         gCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAAGGCCAGCGGGTAAGCaat                                                                                                                                                          >  2:97579/1‑234 (MQ=255)
                                                                          cTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACt                                                                                                                                                          <  1:242299/235‑1 (MQ=255)
                                                                               cgATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGGAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGttt                                                                                                                                                    >  1:533456/1‑236 (MQ=255)
                                                                                 aTCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTa                                                                                                                                                   >  1:385285/1‑235 (MQ=255)
                                                                                     ctctGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGc                                                                                                                                              <  2:479328/236‑1 (MQ=255)
                                                                                                    tgATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTcc                                                                                                                                >  1:203052/1‑235 (MQ=255)
                                                                                                             cacaATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCgcca                                                                                                                      >  1:738133/1‑236 (MQ=255)
                                                                                                             cacaATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGCTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGTTAAGAACTGCGTTTACCAGCGTGGGTAATTTTccccaagtgcca                                                                                                                      >  2:234045/1‑227 (MQ=255)
                                                                                                                       cGTAATCCCCCATGTCTTCGCCGCGCTCCTGCGCCTGATTCAGGGTTTCCGCAAGACTCATCCCATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGcc                                                                                                            <  1:358381/236‑1 (MQ=255)
                                                                                                                          aaTCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTGCCAAGTCGCCAGCCAAAGGCCGAc                                                                                                         >  1:22359/1‑236 (MQ=255)
                                                                                                                                 cATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTa                                                                                                                                                                                                             <  1:550026/130‑1 (MQ=255)
                                                                                                                                 cATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTa                                                                                                                                                                                                             >  2:550026/1‑130 (MQ=255)
                                                                                                                                 cATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGtt                                                                                                  >  1:262098/1‑236 (MQ=255)
                                                                                                                                   tGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCACCGGATAAGCACTGCGTTTACAAGCGTGGGGAATTTTCCCAAATAGCCAGCCCAAGGCCGCCTGGAGGTcc                                                                                                >  2:740040/1‑235 (MQ=255)
                                                                                                                                      cttctCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACgg                                                                                             >  1:20203/1‑236 (MQ=255)
                                                                                                                                         ctcCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGGACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGGTCACGGc                                                                                            >  2:764814/1‑234 (MQ=255)
                                                                                                                                            cgcgCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCaaa                                                                                         >  1:439958/1‑234 (MQ=255)
                                                                                                                                                    tGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGt                                                                                <  1:97579/235‑1 (MQ=255)
                                                                                                                                                      cgcCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCa                                                                                                                                                            <  1:392265/157‑1 (MQ=255)
                                                                                                                                                      cgcCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCa                                                                                                                                                            >  2:392265/1‑157 (MQ=255)
                                                                                                                                                      cgcCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCAc                                                                             <  2:385285/236‑1 (MQ=255)
                                                                                                                                                                  ggTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGacaac                                                                  >  2:296565/1‑235 (MQ=255)
                                                                                                                                                                      tCCGCAAGCCTCATCCCCTCTCGCATTCCGATGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCCCCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGacaaca                                                                 <  2:20203/232‑1 (MQ=255)
                                                                                                                                                                      tCCGCAAGACTCAGCACATCTCGCATACCGAGGTTAACCCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAacac                                                               <  1:271615/233‑1 (MQ=255)
                                                                                                                                                                        cGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACctct                                                           >  2:463212/1‑235 (MQ=255)
                                                                                                                                                                            aGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTcgcg                                                      <  1:296565/236‑1 (MQ=255)
                                                                                                                                                                              aCTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCgcgc                                                     <  1:293544/235‑1 (MQ=255)
                                                                                                                                                                               cTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGAAATTTTCCCAAGTGGCAGGCCAAAGGCCGCCTGGAGTTCACGGAAAAACTTTCCGTCACTCCACACCACACCCTCTTCTGGCCg                                                   >  1:170852/1‑236 (MQ=255)
                                                                                                                                                                                  atcaCATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGTGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCg                                                   <  1:354367/233‑1 (MQ=255)
                                                                                                                                                                                  atcaCATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGTGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCg                                                   >  2:354367/1‑233 (MQ=255)
                                                                                                                                                                                             cATACCGAGTTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGa                                         <  2:213086/232‑1 (MQ=255)
                                                                                                                                                                                             cATACCGAGGTTAAGCCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGAt                                        <  1:740040/233‑1 (MQ=255)
                                                                                                                                                                                             cATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGa                                         >  1:213086/1‑232 (MQ=255)
                                                                                                                                                                                                   gAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACaccaga                               <  2:203052/236‑1 (MQ=255)
                                                                                                                                                                                                   gAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACaccaga                               <  1:63391/236‑1 (MQ=255)
                                                                                                                                                                                                    aGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACaccaga                               >  2:499164/1‑235 (MQ=255)
                                                                                                                                                                                                               ttGCCCGGAAATCGGGTGCAGGGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTACCGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGc                   <  1:721974/236‑1 (MQ=255)
                                                                                                                                                                                                                                 cAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCCGACATCGGc  >  1:618015/1‑235 (MQ=255)
                                                                                                                                                                                                                                 cAGAGTGTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGc  <  1:499164/235‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      
GTTGCGCCCGACAACCAGCGGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGC  >  NC_000913/3052421‑3052880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: