Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,174,612	A→C	L262R (CTG→CGG)	parE ←	DNA topoisomerase IV, subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,174,612	0	A	C	100.0%	178.6 / NA	50	L262R (CTG→CGG)	parE	DNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/25);  total (25/25)

TTCGTCTGCCCGGCAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACAT  >  NC_000913/3174396‑3174840                                                                                                                                                                                                                          |                                                                                                                                                                                                                                     ttCGTCTGCCCGGCAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAg                                                                                                                                                                                                                   <  1:435836/236‑1 (MQ=255) tgtgtgtggccGGCAAACTACGCGTCCTGCATTTTAACCGACGGCCCATAGGCGGAGCGATCCCAGATATCTTCCGCCGACAGCTTTACCCCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTCCC‑GCCCTACATCGTTGGGATAAGGTGGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGc                                                                                                                                                                                                                     <  2:691741/225‑1 (MQ=255)    gTCTGGCCGGCAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCca                                                                                                                                                                                                                <  1:541658/236‑1 (MQ=255)        gCCCGGCAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAg                                                                                                                                                                                                             >  2:72151/1‑235 (MQ=255)        gCCCGGCAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCAACGCCATCCGGCAGCGACAg                                                                                                                                                                                                             >  2:743897/1‑235 (MQ=255)              cAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGcc                                                                                                                                                                                                      <  1:72151/236‑1 (MQ=255)                         tCCTGCATTTTTACGGAACGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCca                                                                                                                                                                                               <  2:90793/232‑1 (MQ=255)                         tCCTGCATTTTGACTGACAGCACATAGGCCCAGCGATCCCAGATATCTTCCGCCGACAGCTTGACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCca                                                                                                                                                                                               <  2:365535/232‑1 (MQ=255)                             gCATTTTTACTGACAGCCCATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGcc                                                                                                                                                                                                      <  2:394606/221‑1 (MQ=255)                             gCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGcc                                                                                                                                                                                                      >  1:394606/1‑221 (MQ=255)                                                 tAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTa                                                                                                                                                                     <  1:743897/234‑1 (MQ=255)                                                          cGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGCTAAACgg                                                                                                                                                          >  2:322538/1‑236 (MQ=255)                                                              cccAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCca                                                                                                                                                                                                                >  2:641230/1‑178 (MQ=255)                                                              cccAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCca                                                                                                                                                                                                                <  1:641230/178‑1 (MQ=255)                                                                       cTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCCTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGc                                                                                                                                             >  2:85444/1‑236 (MQ=255)                                                                         tCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCg                                                                                                                                            >  2:600137/1‑235 (MQ=255)                                                                                              cgcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGGATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCtt                                                                                                                       >  1:677512/1‑235 (MQ=255)                                                                                                    aGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGc                                                                                                                                                                                                                     >  2:388490/1‑135 (MQ=255)                                                                                                    aGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGc                                                                                                                                                                                                                     <  1:388490/135‑1 (MQ=255)                                                                                                        tatTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTaa                                                                                                            >  1:326521/1‑236 (MQ=255)                                                                                                          ttGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTaa                                                                                                            <  2:217175/234‑1 (MQ=255)                                                                                                                              aCGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTa                                                                                                             >  1:712521/1‑213 (MQ=255)                                                                                                                              aCGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTa                                                                                                             <  2:712521/213‑1 (MQ=255)                                                                                                                                   tCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCAcc                                                                                   >  2:38819/1‑234 (MQ=255)                                                                                                                                   tCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAg                                                                                 >  1:755687/1‑236 (MQ=255)                                                                                                                                     gcgTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCg                                                                               <  2:326521/236‑1 (MQ=255)                                                                                                                                      cgTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGGTTTTTCGGCAGCGTCGTCAGACCCTTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAACgg                                                                              >  2:426543/1‑235 (MQ=255)                                                                                                                                             ccagCCCTGGCGCAGTCCATTAACCTGGGTACC‑GCCCTGCATCCTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCg                                                                        <  2:755687/232‑1 (MQ=255)                                                                                                                                             cAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCgg                                                                       <  1:38819/236‑1 (MQ=255)                                                                                                                                                        accctccATTGACCTGGGTGCCGGCCTTGCATCGTTGGGAGAAGGTGACCGTACCTTTCGGTCAGCCGTTCCCCGCCTGCCGGCAGCCACAGGAGCCCCCAGTCCACAGCTTCAGTATCACCCGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGAt                                                             <  2:724894/231‑1 (MQ=255)                                                                                                                                                          aGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGAtctc                                                          <  1:215109/236‑1 (MQ=255)                                                                                                                                                           gACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCa                                                         >  1:685521/1‑236 (MQ=255)                                                                                                                                                                 tAACATCGGTCAC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTa                                                   <  2:403770/236‑1 (MQ=255)                                                                                                                                                                        ggTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGATGATCTCATCTTTAAAAGTg                                             >  1:294577/1‑235 (MQ=255)                                                                                                                                                                                cccTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAAc                                     >  1:340798/1‑236 (MQ=255)                                                                                                                                                                                   tGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGcc                                  >  2:224515/1‑236 (MQ=255)                                                                                                                                                                                    gCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCa                                 <  2:340798/236‑1 (MQ=255)                                                                                                                                                                                    gCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCa                                 <  1:600137/236‑1 (MQ=255)                                                                                                                                                                                     cATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGa                                                                                                   <  2:393882/169‑1 (MQ=255)                                                                                                                                                                                     cATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGa                                                                                                   <  2:326687/169‑1 (MQ=255)                                                                                                                                                                                     cATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGa                                                                                                   >  1:393882/1‑169 (MQ=255)                                                                                                                                                                                     cATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGa                                                                                                   >  1:326687/1‑169 (MQ=255)                                                                                                                                                                                       accttGGGATAAGGTTGACGTTGCTTTCGGTCAGCCGTTCCCCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAggg                              <  1:350302/233‑1 (MQ=255)                                                                                                                                                                                                  aGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAgc                                                                                                                                                                                                         >  1:750599/1‑54 (MQ=255)                                                                                                                                                                                                  aGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAgc                                                                                                                                                                                                         <  2:750599/54‑1 (MQ=255)                                                                                                                                                                                                    gttgcagTAGCTCTCCGTCAGCCGTTCACCGTCTTCCGGCAGCCACAGTAGCGCCCGGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAATCGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCtt                 <  2:294577/230‑1 (MQ=255)                                                                                                                                                                                                        aCGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGc             >  1:706575/1‑236 (MQ=255)                                                                                                                                                                                                         cGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGCTAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAAAGCCATGGCACATTACCGCTTTGGCt            >  1:317372/1‑236 (MQ=255)                                                                                                                                                                                                              cTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACTAGCGTTGTTCGGGATTGTTGATCTCATCCTTAAAACTGATTTCTACTCCAGGGCACATTACCGTTTTGGCTTTCat       >  2:622732/1‑235 (MQ=255)                                                                                                                                                                                                                   ggTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACAt  >  1:65499/1‑236 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                     TTCGTCTGCCCGGCAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACAT  >  NC_000913/3174396‑3174840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑