Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,198,644 A→C F333C (TTC→TGC)  glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,198,6440AC98.6% 238.8 / NA 70F333C (TTC→TGC) glnEfused deadenylyltransferase/adenylyltransferase for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (30/39);  total (31/39)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

ACGCCAGCCGCGCACGATTAAGCTCATCAGAAGGAAGCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGAACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGC  >  NC_000913/3198423‑3198859
                                                                                                                                                                                                                             |                                                                                                                                                                                                                       
aCGCCAGCCGCGCACGATTAAGCTCATCAGAAGGAAGCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATa                                                                                                                                                                                                           >  1:579966/1‑236 (MQ=255)
aCGCCAGCCGCGCACGATTAAGCTCATCAGAAGGAAGCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATa                                                                                                                                                                                                           >  1:541113/1‑236 (MQ=255)
          cgcACGATTAAGCTCATCAGAAGGAAGCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAAttt                                                                                                                                                                                                 >  1:106155/1‑236 (MQ=255)
            cACGATTAAGCTCATCAGAAGGAAGCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGa                                                                                                                                                                                                                 <  2:336795/218‑1 (MQ=255)
            cACGATTAAGCTCATCAGAAGGAAGCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGa                                                                                                                                                                                                                 >  1:336795/1‑218 (MQ=255)
            cACGATTAAGCTCATCAGAAGGAAGCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTcg                                                                                                                                                                                               <  1:754839/236‑1 (MQ=255)
            cACGATTAAGCTCATCAGAAGGAAGCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTcg                                                                                                                                                                                               <  1:5009/236‑1 (MQ=255)
                         atcaGAAGGAAGCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCt                                                                                                                                                                                   >  1:179376/1‑235 (MQ=255)
                           caGAAGGAAGCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTgcg                                                                                                                                                                                >  1:95377/1‑236 (MQ=255)
                             gaaggaagCTTCTGGTTTGGGTCGTCGTTAAGGCTTGGCAGCAGGTTGTCCAGACGCCGAAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGcc                                                                                                                                                                              <  2:639662/236‑1 (MQ=255)
                             gaaggaagCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTGCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGcc                                                                                                                                                                              <  1:647437/236‑1 (MQ=255)
                               aggaagCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGa                                                                                                                                                                            <  2:179376/236‑1 (MQ=255)
                                ggaagCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGAACACCTGAACGATAAATTCCATTTCGCGAATGCCTCCTGCGCCGac                                                                                                                                                                           >  2:142992/1‑235 (MQ=255)
                                  aagCGTCTGGGTTTGTTCGTCGTTAATGCTTTGGAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTGCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGcc                                                                                                                                                                              <  1:151926/231‑1 (MQ=255)
                                  aagCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAg                                                                                                                                                                           <  2:579966/234‑1 (MQ=255)
                                     cGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCGGCCTGGTCCGAGCTTg                                                                                                                                                                       >  2:151926/1‑235 (MQ=255)
                                     cGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGa                                                                                                                                                                      >  2:434876/1‑236 (MQ=255)
                                        cTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGatat                                                                                                                                                                   >  1:3736/1‑236 (MQ=255)
                                         tGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATAtt                                                                                                                                                                  <  1:291312/236‑1 (MQ=255)
                                               tGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGt                                                                                                                                                            >  2:247189/1‑236 (MQ=255)
                                                gTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATTCGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGt                                                                                                                                                            <  1:86735/235‑1 (MQ=255)
                                                      cgtTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCa                                                                                                                                                     <  1:434876/236‑1 (MQ=255)
                                                      cgtTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCa                                                                                                                                                     <  2:272265/236‑1 (MQ=255)
                                                         tAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGt                                                                                                                                                  <  2:218955/236‑1 (MQ=255)
                                                                  gcagcaGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCAc                                                                                                                                          >  2:671639/1‑235 (MQ=255)
                                                                  gcagcaGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCAc                                                                                                                                          >  1:725905/1‑235 (MQ=255)
                                                                     gcagGTTTGCCATACTCGGCAGGAACAGATACCCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCa                                                                                                                                      <  2:536871/236‑1 (MQ=255)
                                                                           tttCCAGACACCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCa                                                                                                                                <  2:481831/236‑1 (MQ=255)
                                                                             tCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCCCTTCACGGGCaa                                                                                                                               >  1:759209/1‑235 (MQ=255)
                                                                              ccAGACGCTGAAGGAACAGATACGCCACTCGCAATGGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAatc                                                                                                                             <  2:725905/236‑1 (MQ=255)
                                                                              ccAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAat                                                                                                                              <  1:671639/235‑1 (MQ=255)
                                                                                       gcAGGAACAGAAACGCCGCTCGAAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCtt                                                                                                                      <  2:106155/234‑1 (MQ=255)
                                                                                              cAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTgt                                                                                                             >  2:319208/1‑235 (MQ=255)
                                                                                                 tatcGCCACTCACAATTGGTCAGCATCGTTGTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAAGGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTgcgc                                                                                                           <  2:393652/232‑1 (MQ=255)
                                                                                                   aCGCCACGCGAAATTCGTCAGCAGCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGc                                                                                                        <  2:3736/236‑1 (MQ=255)
                                                                                                     gCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCa                                                                                                          <  1:49821/232‑1 (MQ=255)
                                                                                                     gCCAATCGCAATTGTTCAGGATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCa                                                                                                          <  1:96294/232‑1 (MQ=255)
                                                                                                         cTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGt                                                                                                                                                                >  2:507679/1‑174 (MQ=255)
                                                                                                         cTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGt                                                                                                                                                                <  1:507679/174‑1 (MQ=255)
                                                                                                          tCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGa                                                                                                  >  2:450323/1‑235 (MQ=255)
                                                                                                           cGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAAt                                                                                                <  1:319208/236‑1 (MQ=255)
                                                                                                                     cAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGaaa                                                                                      >  2:651926/1‑236 (MQ=255)
                                                                                                                             ttttCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATAATCCCTTTCATGTTCCGCAGCGACTGAATCACGCTGAAAACGATgg                                                                               >  2:368449/1‑234 (MQ=255)
                                                                                                                                tCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAacg                                                                           >  1:502658/1‑236 (MQ=255)
                                                                                                                                  aGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGACCTTCACGGGCAATCATCCCTTTCATGTTTGTCACCGGCTGAATCACGCTGAAATGCATGTAacaca                                                                         >  2:158368/1‑233 (MQ=255)
                                                                                                                                           aTGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAaaacaa                                                                >  1:258854/1‑236 (MQ=255)
                                                                                                                                                gCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACggg                                                           <  2:258854/236‑1 (MQ=255)
                                                                                                                                                  tCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAAAGACGGAAAACAAACGGgc                                                          >  1:634790/1‑235 (MQ=255)
                                                                                                                                                     gCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAaaacaaac                                                              <  1:450323/228‑1 (MQ=255)
                                                                                                                                                                gAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGCAATCGATGTAACGACGGAAAACAAACGGGGGCAGCAtagacagaa                                           >  2:51060/1‑228 (MQ=255)
                                                                                                                                                                gAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATcgcacgca                                           >  1:602080/1‑236 (MQ=255)
                                                                                                                                                                  gacgttgCAGTAAAGGGCGCGTTTGCAGGGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAATGATAAATTCAATTTCGCGAATGCCGGCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAAGCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGGGCAGCATCGCACGCaa                                          <  2:95377/229‑1 (MQ=255)
                                                                                                                                                                  gCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCaa                                          <  1:651926/235‑1 (MQ=255)
                                                                                                                                                                  gCGTCGGCAGTAAAGGGCGCGAGTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCaa                                          <  2:759209/235‑1 (MQ=255)
                                                                                                                                                                       gacAGTAAAGGCCCCAATTGCACGGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGt                                     <  2:602080/233‑1 (MQ=255)
                                                                                                                                                                           gTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATcgca                                               >  2:686195/1‑221 (MQ=255)
                                                                                                                                                                           gTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATcgca                                               <  1:686195/221‑1 (MQ=255)
                                                                                                                                                                                gcgcccgATTACAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAg                             <  2:541113/229‑1 (MQ=255)
                                                                                                                                                                                         tGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCt                  <  1:142992/236‑1 (MQ=255)
                                                                                                                                                                                           cAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGACGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTa                 >  1:729238/1‑235 (MQ=255)
                                                                                                                                                                                            aGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTAt                <  2:585922/235‑1 (MQ=255)
                                                                                                                                                                                                        cgTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATAtt                                                                                                                                                                  >  1:166007/1‑77 (MQ=255)
                                                                                                                                                                                                        cgTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATAtt                                                                                                                                                                  <  2:166007/77‑1 (MQ=255)
                                                                                                                                                                                                         gTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCcgc  <  1:247189/236‑1 (MQ=255)
                                                                                                                                                                                                              ccgcGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAacga                                                                          >  2:596003/1‑159 (MQ=255)
                                                                                                                                                                                                              ccgcGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAacga                                                                          <  1:596003/159‑1 (MQ=255)
                                                                                                                                                                                                              ccgcGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCaa                                          >  1:66719/1‑191 (MQ=255)
                                                                                                                                                                                                              ccgcGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCaa                                          <  2:66719/191‑1 (MQ=255)
                                                                                                                                                                                                              ccgcGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTAt                <  1:77764/217‑1 (MQ=255)
                                                                                                                                                                                                              ccgcGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTAt                >  2:77764/1‑217 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                       
ACGCCAGCCGCGCACGATTAAGCTCATCAGAAGGAAGCGTCTGGGTTTGTTCGTCGTTAATGCTTTGCAGCAGGTTTTCCAGACGCCGCAGGAACAGATACGCCACTCGCAATTGTTCAGCATCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGAACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCCCTTTCATGTTGCGCAGCGACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGC  >  NC_000913/3198423‑3198859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: