Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,271,880 T→G E291A (GAA→GCA)  garR ← tartronate semialdehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,271,8800TG98.2% 195.7 / NA 57E291A (GAA→GCA) garRtartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (30/26);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCAC  >  NC_000913/3271669‑3272112
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        
tgCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgtagt                                                                                                                                                                                                                  >  2:20216/1‑236 (MQ=255)
  cATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGtagtag                                                                                                                                                                                                                <  2:740570/236‑1 (MQ=255)
  cATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGtagtag                                                                                                                                                                                                                <  1:20216/236‑1 (MQ=255)
       ggAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGc                                                                                                                                                                                                           >  2:478749/1‑236 (MQ=255)
            aaTTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCa                                                                                                                                                                                                         <  2:344607/233‑1 (MQ=255)
             aTTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATATCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAggg                                                                                                                                                                                                      <  1:444472/235‑1 (MQ=255)
                         ccTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATccgc                                                                                                                                                                                         <  2:397598/236‑1 (MQ=255)
                           tttttCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATccgccg                                                                                                                                                                                       <  1:296741/236‑1 (MQ=255)
                                  aTCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTaa                                                                                                                                                                                >  1:745811/1‑236 (MQ=255)
                                               ccTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGc                                                                                                                                                                   >  1:318832/1‑236 (MQ=255)
                                                   gCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTg                                                                                                                                                               <  1:478749/236‑1 (MQ=255)
                                                    cTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCa                                                                                                                                                                  >  1:169209/1‑232 (MQ=255)
                                                               tctttctgtAAAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCAGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                                                                                       <  2:318832/231‑1 (MQ=255)
                                                               attttctttATAGGGTTCTGGGGCGATTCCGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatcat                                                                                                                                                     <  2:169209/232‑1 (MQ=255)
                                                                   tctttATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGgcg                                                                                                                                                                                                    <  2:166123/183‑1 (MQ=255)
                                                                   tctttATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGgcg                                                                                                                                                                                                    >  1:166123/1‑183 (MQ=255)
                                                                               tCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAg                                                                                                                                                                                                                             >  2:71188/1‑146 (MQ=255)
                                                                               tCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCAGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGCTTTTCGTAGTAGAACGCCAGGGCGCTATGATCTGCCGTTCCTAAACCATCTGCTCGCAGTGACTGCATCATCTCACTAACCGCAGCtg                                                                                                                                    >  1:358560/1‑235 (MQ=255)
                                                                                 tGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCCAGGCGCTATGATCCTCCGTTCCTAAACCCTCTGCTCCCCGTGCCTGCATCATTCCCATAAcagtaacattag                                                                                                                                 >  2:241570/1‑225 (MQ=255)
                                                                                 tGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCCTAACCGCAGCTCTGa                                                                                                                                 >  1:620062/1‑236 (MQ=255)
                                                                                       gATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgt                                                                                                                                                                                                                     <  1:71188/146‑1 (MQ=255)
                                                                                        aTTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAg                                                                                                                                       <  2:424808/223‑1 (MQ=255)
                                                                                        aTTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAg                                                                                                                                       >  1:424808/1‑223 (MQ=255)
                                                                                                    atcccTATCCCTGTTACCACATTACGCCAGATGTCTTTTCTCTTATTGGCCCTATATTCCAGACCCTGATTGCAATACGCCATCCGGACACGACTTCATTAACGAGTAACTGCGACTTTCCCCATTTTTTCTTAGTACCACGCCAGGGCCCTATTATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTTTGAGCGGCAGTTGTGCGCCGAc                                                                                                              <  2:159344/233‑1 (MQ=255)
                                                                                                         tATGCCTGTTACCCCATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                                                                                         <  1:109001/236‑1 (MQ=255)
                                                                                                         tATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTCTGACCGGCAGTTGTGCGCCGACGCCGt                                                                                                         >  1:147101/1‑236 (MQ=255)
                                                                                                           tGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTCTGCGCCGACACCGTgc                                                                                                       >  1:92582/1‑235 (MQ=255)
                                                                                                             ccTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGTGCATGCCGTgaga                                                                                                     >  2:625135/1‑236 (MQ=255)
                                                                                                                gTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAg                                                                                                   >  1:79760/1‑235 (MQ=255)
                                                                                                                 ttACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTa                                                                                                 >  1:90461/1‑236 (MQ=255)
                                                                                                                             gCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCTATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAg                                                                                                                                                                 <  1:699120/160‑1 (MQ=255)
                                                                                                                             gCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCTATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAg                                                                                                                                                                 >  2:699120/1‑160 (MQ=255)
                                                                                                                                  agcgCGTTTCCGTTTATCGGCCTGGATTCCAGGCCCGGATTGAAATCCGCCATCCGGGCACGACCTCATTAACGATTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAg                                                                                <  2:92582/233‑1 (MQ=255)
                                                                                                                                          tctcTTATTCAGCCTGGATTCCAGGCCCGGTTTGACATCCGCCATCCGGGAACGACGTCATTAACGGGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTa                                                                         <  2:560538/235‑1 (MQ=255)
                                                                                                                                              ttATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATg                                                                    <  1:515070/236‑1 (MQ=255)
                                                                                                                                                 tacGGCCTGGATTCCAGGCCCGGATTGCAATGCGCAATCCGGGATCGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAg                                                                 <  2:358560/234‑1 (MQ=255)
                                                                                                                                                   cGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCa                                                                                 >  1:483164/1‑218 (MQ=255)
                                                                                                                                                   cGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCa                                                                                 <  2:483164/218‑1 (MQ=255)
                                                                                                                                                      ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCaa                                                            >  1:6567/1‑236 (MQ=255)
                                                                                                                                                      ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCaa                                                            >  1:165925/1‑236 (MQ=255)
                                                                                                                                                      ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAACGCATTTGCCAGATCCCTAATATGCAGATCaa                                                            >  2:520005/1‑236 (MQ=255)
                                                                                                                                                              ccgggCCAGGATTGAAATCCGCCAGTCGGGCACAGAGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGaa                                                     <  2:620062/232‑1 (MQ=255)
                                                                                                                                                               cAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCgg                                                                                                                             <  1:690149/162‑1 (MQ=255)
                                                                                                                                                               cAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCgg                                                                                                                             >  2:690149/1‑162 (MQ=255)
                                                                                                                                                                     cGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATACAGGGCATTCGCCAGAACCTTAACATGCAGATCAATACGGAAGCCCAGCt                                             >  2:43583/1‑236 (MQ=255)
                                                                                                                                                                      ggATTGCAATCCGCAATCCGGGCACGGCGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCg                                                <  2:645923/232‑1 (MQ=255)
                                                                                                                                                                                 cGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGCGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCGGCTTGAAGTTGCgg                                  >  1:694516/1‑235 (MQ=255)
                                                                                                                                                                                    cATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt                                                                              >  1:258831/1‑188 (MQ=255)
                                                                                                                                                                                    cATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt                                                                              <  2:258831/188‑1 (MQ=255)
                                                                                                                                                                                      tCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCCATACGGAAGCCCGGCTTGAAGTTGCGGTCCAt                             >  2:489284/1‑235 (MQ=255)
                                                                                                                                                                                                     aTTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGcc                                                  <  1:759151/199‑1 (MQ=255)
                                                                                                                                                                                                     aTTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGcc                                                  >  2:759151/1‑199 (MQ=255)
                                                                                                                                                                                                        aaCGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCa          <  1:625135/236‑1 (MQ=255)
                                                                                                                                                                                                         aCGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCa          >  1:83854/1‑235 (MQ=255)
                                                                                                                                                                                                              tAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatcat                                                                                                                                                     <  1:309915/91‑1 (MQ=255)
                                                                                                                                                                                                              tAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatcat                                                                                                                                                     >  2:309915/1‑91 (MQ=255)
                                                                                                                                                                                                                aCTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATAACCATCGGCGCTTTGGCATCCAGCAc  >  1:678728/1‑236 (MQ=255)
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        
TGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCAC  >  NC_000913/3271669‑3272112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: