Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,327,851	T→G	V21G (GTT→GGT)	yhbY →	RNA binding protein associated with pre‑50S ribosomal subunits

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,327,851	0	T	G	100.0%	272.6 / NA	74	V21G (GTT→GGT)	yhbY	RNA binding protein associated with pre‑50S ribosomal subunits
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (38/36);  total (38/36)

GCCAGCGGCTGGAGCTGGCAGAACGCTTCTTACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCT  >  NC_000913/3327625‑3328081                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       gCCAGCGGCTGGAGCTGGCAGAACGCTTCTTACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAAAAGCACCTGAAAGGACTGGGACATCCGCTCAAGCCAGGTGTTCTGCt                                                                                                                                                                                                                               >  2:18975/1‑236 (MQ=255)          tGGAGCTGGCAGAACGCTTCTTACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTgg                                                                                                                                                                                                                            <  1:572489/230‑1 (MQ=255)              gCTGGCAGAACGCTTCTTACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGt                                                                                                                                                                                                                  <  1:544739/236‑1 (MQ=255)                 ggCAGAACGCTTCTTACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGttt                                                                                                                                                                                                                <  2:274194/235‑1 (MQ=255)                 ggCAGAACGCTTCTTACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGttt                                                                                                                                                                                                                <  1:18975/235‑1 (MQ=255)                            tcttACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGGGTTCTGCTTGGCAGTAATGGTTTGACCGAAgg                                                                                                                                                                                                       >  2:105508/1‑233 (MQ=255)                                     tCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCga                                                                                                                                                                                           >  2:400327/1‑236 (MQ=255)                                     tCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGACTGGCACATCCGCTCAAACCAGGGGTTCTGCTTGGCAGCAATGGTTTGACCGAAgggggacgggtcga                                                                                                                                                                                           >  2:728406/1‑226 (MQ=255)                                       aTTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCgaga                                                                                                                                                                                         <  2:516189/236‑1 (MQ=255)                                               ttCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGa                                                                                                                                                                                     >  1:688014/1‑232 (MQ=255)                                                tCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAg                                                                                                                                                                                <  1:22116/236‑1 (MQ=255)                                                       gggATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTaga                                                                                                                                                                         <  1:618995/236‑1 (MQ=255)                                                             aCTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCAcc                                                                                                                                                                    <  2:421110/235‑1 (MQ=255)                                                                cATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGa                                                                                                                                                                <  1:27592/236‑1 (MQ=255)                                                                 aTCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGaa                                                                                                                                                               >  2:603698/1‑236 (MQ=255)                                                                 aTCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGaa                                                                                                                                                               >  2:635386/1‑236 (MQ=255)                                                                      aaCCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACtcat                                                                                                                                                          <  1:635386/236‑1 (MQ=255)                                                                       aCCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACtcatc                                                                                                                                                         >  2:335029/1‑236 (MQ=255)                                                                        ccAATTGCGTAAATTTTTACGCGCCTATTTGGTGATTTATGGGAGATTGCCGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGGCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACtcatca                                                                                                                                                        <  2:633600/236‑1 (MQ=255)                                                                        ccAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACtcatca                                                                                                                                                        >  1:431475/1‑236 (MQ=255)                                                                            ttGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGt                                                                                                                                                    >  1:633600/1‑236 (MQ=255)                                                                             tGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTg                                                                                                                                                   >  1:385919/1‑236 (MQ=255)                                                                               cGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGa                                                                                                                                                                                                              <  1:487555/175‑1 (MQ=255)                                                                               cGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGa                                                                                                                                                                                                              >  2:487555/1‑175 (MQ=255)                                                                                   aaTTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAAt                                                                                                                                              <  1:400327/235‑1 (MQ=255)                                                                                                    ttGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGa                                                                                                                            <  1:603698/236‑1 (MQ=255)                                                                                                    ttGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGa                                                                                                                            <  2:508489/236‑1 (MQ=255)                                                                                                             tatGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAAc                                                                                                                   >  2:242556/1‑236 (MQ=255)                                                                                                             tatGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAAc                                                                                                                   >  1:108517/1‑236 (MQ=255)                                                                                                             tatGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGAGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGCTTGAACCAGCGTTATAGCACCCTGAACTCATCATAGGCAAAATCGCCAGCGAAGAGCGAGAAACTa                                                                                                                       >  2:396402/1‑232 (MQ=255)                                                                                                                  gagaTGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGa                                                                                                              >  1:567905/1‑236 (MQ=255)                                                                                                                     aTGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGa                                                                                                                            <  1:125574/219‑1 (MQ=255)                                                                                                                     aTGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGa                                                                                                                            >  2:125574/1‑219 (MQ=255)                                                                                                                     aTGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATct                                                                                                           >  1:28827/1‑235 (MQ=255)                                                                                                                       ggCGGTAGAATGCCCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAGCAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGt                                                                                                          <  1:825/235‑1 (MQ=255)                                                                                                                       ggCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTg                                                                                                         >  2:685222/1‑236 (MQ=255)                                                                                                                       ggCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTg                                                                                                         >  1:389576/1‑236 (MQ=255)                                                                                                                       ggCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTg                                                                                                         >  2:174246/1‑236 (MQ=255)                                                                                                                       ggCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTg                                                                                                         >  1:425512/1‑236 (MQ=255)                                                                                                                                   aCCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCCGAAAGGTCTGGCACATCCGCTCAAGCCAGGGGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGCGATTGAACAAGCGTTAGAGCAACATGAACCCATCAAGTGGGAAATCGCCACCGAAGGTCGCGAAACTAAAACCTTTATAATGGAAGCTactgtt                                                                                             >  2:9234/1‑231 (MQ=255)                                                                                                                                         tttCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGaa                                                                                       >  2:498162/1‑236 (MQ=255)                                                                                                                                         tttCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGAGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGaa                                                                                       >  1:541936/1‑236 (MQ=255)                                                                                                                                              aTCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCgg                                                                                  <  1:728406/236‑1 (MQ=255)                                                                                                                                              aTCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCgg                                                                                  <  2:567905/236‑1 (MQ=255)                                                                                                                                               tCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGgc                                                                                 <  2:425512/236‑1 (MQ=255)                                                                                                                                                cccAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATAGTGGAATCTATCGTGCGCGAAACCg                                                                                   >  2:377631/1‑233 (MQ=255)                                                                                                                                                 ccAGCGTAAGAAAAAAATACGATGAATCTGAATACTAAACAAAAACAGCACCGGAAAGGTCTGGCAAATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGgcgc                                                                               <  2:28827/236‑1 (MQ=255)                                                                                                                                                 ccAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGgcgc                                                                               <  1:498162/236‑1 (MQ=255)                                                                                                                                                 ccAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGgcgc                                                                               <  2:389576/236‑1 (MQ=255)                                                                                                                                                 ccAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGgcgc                                                                               <  1:174246/236‑1 (MQ=255)                                                                                                                                                 ccAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGgcgc                                                                               <  1:685222/236‑1 (MQ=255)                                                                                                                                                  cAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGt                                                                                                          >  2:34269/1‑208 (MQ=255)                                                                                                                                                  cAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGt                                                                                                          <  1:34269/208‑1 (MQ=255)                                                                                                                                                           cAAAAAATACGATGAATCTGAATACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTa                                                                     <  2:287743/236‑1 (MQ=255)                                                                                                                                                                   aCGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCAt                                                             <  1:660946/236‑1 (MQ=255)                                                                                                                                                                     gATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTa                                                                     <  1:509120/226‑1 (MQ=255)                                                                                                                                                                              gAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGc                                                  <  2:431475/236‑1 (MQ=255)                                                                                                                                                                               aGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCt                                                 >  2:568133/1‑236 (MQ=255)                                                                                                                                                                                      aaCAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCt                                                 >  1:177692/1‑229 (MQ=255)                                                                                                                                                                                           aaaCAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCATGCGCGAAACCGGCGCCGGAAATGTACAGGTCATCGGTAAAACGCTgggaccttaacg                                     >  2:241452/1‑226 (MQ=255)                                                                                                                                                                                                gCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACtcatc                                                                                                                                                         >  1:285584/1‑115 (MQ=255)                                                                                                                                                                                                gCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACtcatc                                                                                                                                                         <  2:285584/115‑1 (MQ=255)                                                                                                                                                                                                gCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCaa                                >  1:561517/1‑236 (MQ=255)                                                                                                                                                                                                 cACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTAGTGCTTTATCCGCCAAc                               >  2:637463/1‑236 (MQ=255)                                                                                                                                                                                                       aaaGGTCTGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGa                         <  1:242556/236‑1 (MQ=255)                                                                                                                                                                                                              tGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTaaa                           <  1:309343/227‑1 (MQ=255)                                                                                                                                                                                                              tGGCACATCCGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTaaa                           >  2:309343/1‑227 (MQ=255)                                                                                                                                                                                                                gCACATCCGATCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTaaaa                 <  2:561517/235‑1 (MQ=255)                                                                                                                                                                                                                      ccGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGc           >  2:461851/1‑235 (MQ=255)                                                                                                                                                                                                                      ccGCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGAGCTTTATCGCCCAACTCAAGAACGTAAAATCTCGCt          >  1:467624/1‑236 (MQ=255)                                                                                                                                                                                                                        gCTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGc        <  2:177692/236‑1 (MQ=255)                                                                                                                                                                                                                         cTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTaa                                                       >  1:731639/1‑188 (MQ=255)                                                                                                                                                                                                                         cTCAAGCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTaa                                                       <  2:731639/188‑1 (MQ=255)                                                                                                                                                                                                                              gCCAGGTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATTTCGCTGCCACGCt  >  2:455660/1‑236 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       GCCAGCGGCTGGAGCTGGCAGAACGCTTCTTACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCT  >  NC_000913/3327625‑3328081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑