Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,399,779 A→C L67R (CTG→CGG)  mreC ← cell wall structural complex MreBCD transmembrane component MreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,399,7790AC98.7% 256.8 / NA 78L67R (CTG→CGG) mreCcell wall structural complex MreBCD transmembrane component MreC
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (36/41);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAG  >  NC_000913/3399546‑3400007
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                    
cGCCAATAACGCTACCTTTAACGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGc                                                                                                                                                                                                                                    <  2:376361/236‑1 (MQ=255)
  ccATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGt                                                                                                                                                                                                                                  <  2:630856/236‑1 (MQ=255)
      tAACGCTACCTTTATCGATACCAACTTGATCGCTATAAGTATCGTTAACCGTGGAGATAGCCTGAGTCCCCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGt                                                                                                                                                                                                                                  <  1:252635/232‑1 (MQ=255)
                  tATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATcc                                                                                                                                                                                                                  >  2:513382/1‑236 (MQ=255)
                         aacaacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAAtt                                                                                                                                                                                                           >  1:41057/1‑236 (MQ=255)
                                 gcttgCTATAAGGATCGTTAACCGGGGAAATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACgagga                                                                                                                                                                                                   <  2:184341/232‑1 (MQ=255)
                                   tCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTTTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTAACTAGGAGc                                                                                                                                                                                                 >  2:483322/1‑236 (MQ=255)
                                      cTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCAtt                                                                                                                                                                                              >  1:103083/1‑236 (MQ=255)
                                      cTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCAtt                                                                                                                                                                                              <  2:265745/236‑1 (MQ=255)
                                         taAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTggc                                                                                                                                                                                           >  1:734453/1‑235 (MQ=255)
                                         taAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGa                                                                                                                                                                                           >  2:624449/1‑236 (MQ=255)
                                                 gTTAACCGTGGAGATACCCTGAGTCACCATTTTCCTCTCATCCTGACGCAGCGGGGACCCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACaaa                                                                                                                                                                                     <  2:481104/234‑1 (MQ=255)
                                                 gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACgagga                                                                                                                                                                                                   >  2:557224/1‑220 (MQ=255)
                                                 gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACgagga                                                                                                                                                                                                   <  1:557224/220‑1 (MQ=255)
                                                 gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTTGAAACaaatc                                                                                                                                                                                   >  2:577652/1‑234 (MQ=255)
                                                 gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACaaa                                                                                                                                                                                     <  2:327706/234‑1 (MQ=255)
                                                 gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGt                                                                                                                                                                                   >  2:619394/1‑236 (MQ=255)
                                                 gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGt                                                                                                                                                                                   >  2:45673/1‑236 (MQ=255)
                                                 gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGt                                                                                                                                                                                   >  1:481104/1‑236 (MQ=255)
                                                 gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGt                                                                                                                                                                                   >  1:22258/1‑236 (MQ=255)
                                                 gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGCTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCACTGGAAACAAAGt                                                                                                                                                                                   >  1:506129/1‑236 (MQ=255)
                                                       cGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAg                                                                                                                                                                             >  2:504592/1‑236 (MQ=255)
                                                           gagaTAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACt                                                                                                                                                                         >  1:278491/1‑236 (MQ=255)
                                                            agaTAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTg                                                                                                                                                                        <  1:583639/236‑1 (MQ=255)
                                                                  ccTGATTCACCATTTGCTGCTCATCCTGACGCAGCGGTGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGAcggcg                                                                                                                                                                  <  2:18199/236‑1 (MQ=255)
                                                                  ccTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGAcggcg                                                                                                                                                                  <  1:513382/236‑1 (MQ=255)
                                                                  ccTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGAcggcg                                                                                                                                                                  <  2:41057/236‑1 (MQ=255)
                                                                  ccTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGAcggcg                                                                                                                                                                  <  1:619394/236‑1 (MQ=255)
                                                                    tGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGt                                                                                                                                                                <  1:561191/236‑1 (MQ=255)
                                                                    tGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTAGTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGt                                                                                                                                                                <  1:25548/236‑1 (MQ=255)
                                                                       gTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATc                                                                                                                                                             <  1:45673/236‑1 (MQ=255)
                                                                            cATTTTCTGCTCATCCTGACGCAGGGGGGAACCCAGCAGCTCGCGCAGCCGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCatat                                                                                                                                                        <  1:687411/236‑1 (MQ=255)
                                                                            cATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCatat                                                                                                                                                        <  1:504592/236‑1 (MQ=255)
                                                                                 tCTGCTCATCCGAACGCAGCGGGGAACCCAGCATCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTa                                                                                                                                                   <  1:380759/236‑1 (MQ=255)
                                                                                    gCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGt                                                                                                                                                    <  2:726007/232‑1 (MQ=255)
                                                                                    gCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGt                                                                                                                                                    >  1:726007/1‑232 (MQ=255)
                                                                                     cTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGa                                                                                                                                               <  1:624449/236‑1 (MQ=255)
                                                                                     cTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGa                                                                                                                                               >  2:266398/1‑236 (MQ=255)
                                                                                           cTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGAc                                                                                                                                         <  2:103083/236‑1 (MQ=255)
                                                                                               agCAGCGGGAAACCCAGCAGCTCGCGAAGACGCGCGTTCTCCTTGTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGaa                                                                                                                                     <  1:311332/235‑1 (MQ=255)
                                                                                                    cGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCCATGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCa                                                                                                                                                           <  1:551699/209‑1 (MQ=255)
                                                                                                    cGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCa                                                                                                                                                           >  2:551699/1‑209 (MQ=255)
                                                                                                    cGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGt                                                                                                                                                    >  2:32517/1‑216 (MQ=255)
                                                                                                    cGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGt                                                                                                                                                    <  1:32517/216‑1 (MQ=255)
                                                                                                        gAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCa                                                                                                                             <  1:577652/235‑1 (MQ=255)
                                                                                                              agcggcTCGCTCAGACGCGTGTTCGCCTGGTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCt                                                                                                                      <  2:734453/236‑1 (MQ=255)
                                                                                                               gcagcTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTg                                                                                                                     <  1:490363/236‑1 (MQ=255)
                                                                                                                  gcTGGCGCAGCCGAGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCg                                                                                                                  <  2:436077/236‑1 (MQ=255)
                                                                                                                   cTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCgg                                                                                                                 >  2:371676/1‑236 (MQ=255)
                                                                                                                       cgcAGACGCGGGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCaa                                                                                                              <  1:559343/235‑1 (MQ=255)
                                                                                                                       cgcAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGc                                                                                                                <  1:371676/233‑1 (MQ=255)
                                                                                                                                gcgTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGc                                                                                                     >  2:551286/1‑235 (MQ=255)
                                                                                                                                             tGTAGTGACCGAGCTGCAGCAGTCCACTGTTTTTCAGCAACAGGTCCTGGCGTAACGCCCGGTTTTCAAGTTCTAATTGGTTACGCGGGGCCAGCGTCTGAGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCAGATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGc                                                                                       <  2:281304/236‑1 (MQ=255)
                                                                                                                                                aTTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCTCAAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGGAcc                                                                                    >  2:467767/1‑236 (MQ=255)
                                                                                                                                                aTTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCAcc                                                                                    >  1:765125/1‑236 (MQ=255)
                                                                                                                                                    tCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACAGCCa                                                                                >  1:28806/1‑236 (MQ=255)
                                                                                                                                                         gCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGt                                                                                                                    <  1:235086/195‑1 (MQ=255)
                                                                                                                                                         gCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGt                                                                                                                    >  2:235086/1‑195 (MQ=255)
                                                                                                                                                            tCAGCAGTTCACTTTTTTTCAGCAACAGTTCCTGCCGTAACGTCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGc                                                                                                                <  2:732990/196‑1 (MQ=255)
                                                                                                                                                              agcagTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGt                                                                                                                                                                <  2:766592/146‑1 (MQ=255)
                                                                                                                                                              agcagTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGt                                                                                                                                                                >  1:766592/1‑146 (MQ=255)
                                                                                                                                                              agcagTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGACACCAGCACCGCCAGAATAAGGc                                                                       >  1:671664/1‑235 (MQ=255)
                                                                                                                                                                   ttCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCaataata                                                                                                         >  1:732990/1‑196 (MQ=255)
                                                                                                                                                                   ttCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCt                                                                 >  1:286927/1‑236 (MQ=255)
                                                                                                                                                                          tttttCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGa                                                          >  1:214092/1‑236 (MQ=255)
                                                                                                                                                                          tttgtCAGCCACAGTTCCTGACGTAGCGCCCGGTTTTCAAGTTCTAATTGATCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAATTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGa                                                          <  2:214092/236‑1 (MQ=255)
                                                                                                                                                                                aGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGcc                                                    <  1:266398/236‑1 (MQ=255)
                                                                                                                                                                                    aCAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACgg                                                >  1:68265/1‑236 (MQ=255)
                                                                                                                                                                                            tGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGc                                                                                                                <  2:10052/164‑1 (MQ=255)
                                                                                                                                                                                            tGACGTAACGCCCGGTTTGCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCAGCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTaaaaa                                         <  1:467767/235‑1 (MQ=255)
                                                                                                                                                                                                  aaCGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCCATAATAATGCCGAGCGACACCCGCAACGCGCGCATAAAGCCAATCTGTAGCGAATGGACACAGCGCACAATTGGCtt                                  >  2:315051/1‑236 (MQ=255)
                                                                                                                                                                                                     gCCGGGTTTTCAAGTTCTAATTGGACACGCGAGGCCCGCTTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGGGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGGGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCAt                               <  2:671664/236‑1 (MQ=255)
                                                                                                                                                                                                          gTTTTCAAGTTCTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGt                           >  1:176245/1‑235 (MQ=255)
                                                                                                                                                                                                                     cTAATTGGTCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGGATtct               >  1:684012/1‑236 (MQ=255)
                                                                                                                                                                                                                             tCACGCGAGGCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAg       >  1:485167/1‑236 (MQ=255)
                                                                                                                                                                                                                                  cgAGGCCCGAATCAGCGATCCGCCATCAAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGCCGGACGGCGGTATCCATATAAGTCCGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACcag  <  2:684012/236‑1 (MQ=255)
                                                                                                                                                                                                                                      gCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGa                                                                     <  1:429800/165‑1 (MQ=255)
                                                                                                                                                                                                                                      gCCCGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGa                                                                     >  2:429800/1‑165 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                    
CGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTGCGATACGCCATCCAGCAATTCACGAGGAGCATTGGAAACAAAGTAGAAAGGACTGACGGCGGTATCCATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAG  >  NC_000913/3399546‑3400007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: