Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,520,438 A→C L89R (CTC→CGC)  hofQ ← DNA catabolic putative fimbrial transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,520,4380AC100.0% 243.2 / NA 69L89R (CTC→CGC) hofQDNA catabolic putative fimbrial transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/34);  total (35/34)

GCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGG  >  NC_000913/3520205‑3520664
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                  
gcgcgcTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTgagcga                                                                                                                                                                                                                                  <  1:402544/236‑1 (MQ=255)
gcgcgcTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCCATTGGTCCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTgagcga                                                                                                                                                                                                                                  <  1:277762/236‑1 (MQ=255)
          cGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGTCGGGCGGTATTGTTATTCTGCCAGGCAATGGAATGCACTTAGCGAATg                                                                                                                                                                                                                               >  2:404842/1‑229 (MQ=255)
                 ttGTTATCGCGTAGCAAAAGGCGATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCcg                                                                                                                                                                                                                 <  1:211976/236‑1 (MQ=255)
                         tcGTAGCAAAAGGCGATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGTCATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCGTCCGCGTGATCa                                                                                                                                                                                                         >  2:766656/1‑236 (MQ=255)
                         tcGTAGCAAAAGGCGATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCa                                                                                                                                                                                                         >  2:745632/1‑236 (MQ=255)
                              gCAAAAGGCGATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCg                                                                                                                                                                                                    <  1:593604/236‑1 (MQ=255)
                                 aacggCGATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGgcg                                                                                                                                                                                                 <  1:145159/233‑1 (MQ=255)
                                   aGGCTATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCt                                                                                                                                                                                               <  1:404842/236‑1 (MQ=255)
                                   aGGCGATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGgcg                                                                                                                                                                                                 <  1:499125/234‑1 (MQ=255)
                                   aGGCGATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCt                                                                                                                                                                                               <  1:745632/236‑1 (MQ=255)
                                   aGGCGATTGGTGCGTTTAGCGACGGTCATTCTCCCTTGGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCGCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCt                                                                                                                                                                                               <  2:184230/236‑1 (MQ=255)
                                   aGGCAATTGGTCCGTTTAACGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCt                                                                                                                                                                                               <  2:84930/236‑1 (MQ=255)
                                         ttGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCa                                                                                                                                                                                          >  1:184230/1‑235 (MQ=255)
                                          gggTGCGTTTATCGGCGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACt                                                                                                                                                                                        <  1:583556/235‑1 (MQ=255)
                                           ggTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACt                                                                                                                                                                                        <  2:341359/235‑1 (MQ=255)
                                               cGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTa                                                                                                                                                                                       >  1:216138/1‑232 (MQ=255)
                                                       gACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTa                                                                                                                                                                              >  1:404002/1‑233 (MQ=255)
                                                         cGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGcc                                                                                                                                                                         <  2:11123/236‑1 (MQ=255)
                                                          gtgtatacctccTTGGGCACTCAGTAGCTTCCCCCCGGCTTTCGCCAGTTCCCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCt                                                                                                                                                                        <  2:323343/226‑1 (MQ=255)
                                                             cATTCTCCCTTTGGCATTCAGTAGCTTCCCCCCCGCTTTCGCCAGTTCTCCCGGGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGcc                                                                                                                                                                         <  2:158720/232‑1 (MQ=255)
                                                                   cccTTTGGCCTTGAGTACTTTCTCCCCCGTTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAgg                                                                                                                                                               <  2:299706/236‑1 (MQ=255)
                                                                    ccTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTcc                                                                                                                                                                  >  1:158720/1‑232 (MQ=255)
                                                                      tttGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGCTTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTACCAGTCCGGCGCTTTTCACTACCGTTTGTAGTGCCTGCTTCCAGTGa                                                                                                                                                             >  2:82111/1‑235 (MQ=255)
                                                                      tttGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGACTGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTATTGCCTGCTTCCAGGGa                                                                                                                                                             >  1:299706/1‑235 (MQ=255)
                                                                        tGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACa                                                                                                                                                          >  2:160659/1‑236 (MQ=255)
                                                                         ggCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGa                                                                                                                                                             >  1:700197/1‑232 (MQ=255)
                                                                         ggCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGa                                                                                                                                                             >  1:679984/1‑232 (MQ=255)
                                                                         gccattcgttAGCTGCTCCCCCGCTTTCGCCAGTTCTCCCGGGTCGGCTTATTGCAGGGTTATACTGCGATTTCCCAGCGGCAGATTTGCCTGCGCCAGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACAt                                                                                                                                                         <  2:651956/227‑1 (MQ=255)
                                                                            gttCAGTAGCTTCGCCCCTGCTTTCGCCAGTTCCCCCGCGTCCGCGTATTGCAGGGTTATGCTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTg                                                                                                                                                      <  2:519606/234‑1 (MQ=255)
                                                                                aGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCgg                                                                                                                                                                                                   >  1:457120/1‑187 (MQ=255)
                                                                                aGTAGCTTCTCCCCCGCATTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCgg                                                                                                                                                                                                   <  2:457120/187‑1 (MQ=255)
                                                                                 gTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGa                                                                                                                                                 <  1:4432/236‑1 (MQ=255)
                                                                                  tAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGAt                                                                                                                                                <  2:216138/236‑1 (MQ=255)
                                                                                     cTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTa                                                                                                                                             >  1:208232/1‑236 (MQ=255)
                                                                                         tcCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACg                                                                                                                                          >  1:435816/1‑235 (MQ=255)
                                                                                          cccccGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCg                                                                                                                                                                                                    >  2:344063/1‑176 (MQ=255)
                                                                                          cccccGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCg                                                                                                                                                                                                    <  1:344063/176‑1 (MQ=255)
                                                                                                               ccGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGgcg                                                                                                                                                                                                 <  2:490136/158‑1 (MQ=255)
                                                                                                               ccGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGgcg                                                                                                                                                                                                 >  1:490136/1‑158 (MQ=255)
                                                                                                                             tGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACATGACCAGGt                                                                                                      >  1:186818/1‑235 (MQ=255)
                                                                                                                                  ggTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACt                                                                                                                                                                                        >  2:457534/1‑148 (MQ=255)
                                                                                                                                  ggTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACt                                                                                                                                                                                        <  1:457534/148‑1 (MQ=255)
                                                                                                                                    ttATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGCTATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGCATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGGAGATCTGTTAGATGGTACGCACCCGTACCCCTGACGCCGTGTGACACGAACAGGTTCAACt                                                                                                >  2:587196/1‑234 (MQ=255)
                                                                                                                                         cTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTg                                                                                         >  1:269349/1‑236 (MQ=255)
                                                                                                                                            cGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCCGGTTCAACTTCTCCTGtt                                                                                       >  1:618763/1‑235 (MQ=255)
                                                                                                                                            cGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTAAACTTCTCCTGtt                                                                                       >  1:762673/1‑235 (MQ=255)
                                                                                                                                            cGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCCGTTAGCTGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCACCTTATCCCGtt                                                                                       >  2:564521/1‑235 (MQ=255)
                                                                                                                                              aTTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGa                                                                                                            >  1:4421/1‑212 (MQ=255)
                                                                                                                                              aTTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGa                                                                                                            <  2:4421/212‑1 (MQ=255)
                                                                                                                                                   ccAGTGGCAGATTGCCCGCGCCCCGCGCCTGCTCCGCCTCCGGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagccagc                                                                               <  2:208232/236‑1 (MQ=255)
                                                                                                                                                               ttGCCGGCGCCCGGTCCTGCTCCGGCTCCTGGCGGTCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAacac                                                                    <  1:564521/235‑1 (MQ=255)
                                                                                                                                                                 gCCTGCCCCCGCTGCTGCTCCGCCTCCGGGCGGGCGATATTGTTATTCTGCTAGTCAACGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCa                                                                        <  2:435816/229‑1 (MQ=255)
                                                                                                                                                                      cgcCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTa                                                            >  1:270813/1‑236 (MQ=255)
                                                                                                                                                                      cgcCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACAACACCGTACCGCTGACGTCTGGCGACACGACCAGGGTCAACTTCTCCTGTTCAGCCACAGCCTGCAACACCTGAGCTa                                                            >  1:698020/1‑236 (MQ=255)
                                                                                                                                                                       gcCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAAAACCTGAGCTAc                                                           >  1:125275/1‑236 (MQ=255)
                                                                                                                                                                             cgcCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACAt                                                                                                                                                         <  2:20510/137‑1 (MQ=255)
                                                                                                                                                                             cgcCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACAt                                                                                                                                                         >  1:20510/1‑137 (MQ=255)
                                                                                                                                                                                cTGCTCCGCCTCCTGGCGGGCGATTTTGTTATTCTGCCAGGCAATGGAATGCACTGGGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGt                                                   <  1:348371/236‑1 (MQ=255)
                                                                                                                                                                                  tcaccGTCTCCGGGCAGGCGATATTTGTATTCCGCCAGGAAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCTTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCa                                                 <  2:186818/233‑1 (MQ=255)
                                                                                                                                                                                          ttctGGCGGGCGATATGGGTATTCTGCCAGGCAATGGAATGCACTGGGCGAATGTTGCCTTCCGGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCAt                                         <  2:618763/234‑1 (MQ=255)
                                                                                                                                                                                                  ggcgATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTg                                                                                                                                                                                <  2:311650/93‑1 (MQ=255)
                                                                                                                                                                                                  ggcgATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTg                                                                                                                                                                                >  1:311650/1‑93 (MQ=255)
                                                                                                                                                                                                   gcgATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCAc                                >  1:318840/1‑236 (MQ=255)
                                                                                                                                                                                                         ttGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTACTGTTCAGCCACGGCCTGCAAACCCTAAGCTACCGGAACGCAATCCACAATCACCGGCACttttt                           >  2:431621/1‑235 (MQ=255)
                                                                                                                                                                                                                 cTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagc                                                                                   >  2:114136/1‑171 (MQ=255)
                                                                                                                                                                                                                 cTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagc                                                                                   <  1:114136/171‑1 (MQ=255)
                                                                                                                                                                                                                          aaTGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTa         <  1:37730/236‑1 (MQ=255)
                                                                                                                                                                                                                              gAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGcc     <  1:766656/236‑1 (MQ=255)
                                                                                                                                                                                                                              gAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGcc     >  1:661903/1‑236 (MQ=255)
                                                                                                                                                                                                                                 tGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCggg  >  2:482729/1‑236 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                  
GCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGG  >  NC_000913/3520205‑3520664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: