Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,540,754 T→G I116M (ATT→ATG feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,540,7540TG96.8% 216.1 / ‑0.8 63I116M (ATT→ATGfeoBferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (27/34);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

TAAAGAAGGGCAATTCTCCACCACCGATCATCAGGTCACGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACTGGTGCATTACGCACAGCCGCTGCTCAACGAAGCAGATTCACTGGCAAAAGTGATGCC  >  NC_000913/3540526‑3540984
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      
tAAAGAAGGGCAATTCCCCACCCCCGTTCATCAGGTCACGCTGGTGGACCTGCCCGGCCCCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCa                                                                                                                                                                                                                                  <  2:720758/235‑1 (MQ=255)
    gaatGGCAATTCTCCCCCACCGATCATCAGGTCACGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGGGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCa                                                                                                                                                                                                                                  <  2:396162/231‑1 (MQ=255)
          cAATTCTCCACCACCGATCATCAGGTCACGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGa                                                                                                                                                                                                                              <  2:226666/229‑1 (MQ=255)
          cAATTCTCCACCACCGATCATCAGGTCACGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGa                                                                                                                                                                                                                              >  1:226666/1‑229 (MQ=255)
                       ccGATCATCAGGTCACGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCa                                                                                                                                                                                                                                  >  1:33370/1‑212 (MQ=255)
                       ccGATCATCAGGTCACGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCa                                                                                                                                                                                                                                  <  2:33370/212‑1 (MQ=255)
                          atcatcAGGTCACGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAg                                                                                                                                                                                                       >  1:225307/1‑236 (MQ=255)
                                      cGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATAtt                                                                                                                                                                                              <  1:246786/233‑1 (MQ=255)
                                      cGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCCAGCAGCAAAATATTCgg                                                                                                                                                                                           >  2:265089/1‑235 (MQ=255)
                                       gCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTa                                                                                                                                                                                          >  2:209974/1‑236 (MQ=255)
                                       gCTGGTCGCCCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGCCCTCGCTCGATGGGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTa                                                                                                                                                                                          <  2:563291/236‑1 (MQ=255)
                                           gtggACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGa                                                                                                                                                                                      <  1:665299/236‑1 (MQ=255)
                                           gtggACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGa                                                                                                                                                                                      >  2:522268/1‑236 (MQ=255)
                                                   gCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATg                                                                                                                                                                              <  1:598156/236‑1 (MQ=255)
                                                             tattccacgACCACCATCTCGTCGCGAGCCTCGCTCGATGAGCAAATCCCCTGTCACTACATTTTGAGTGGCGCCGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCgg                                                                                                                                                                     <  2:225307/227‑1 (MQ=255)
                                                             tATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCgg                                                                                                                                                                     <  1:209974/235‑1 (MQ=255)
                                                             tATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCgg                                                                                                                                                                     <  1:173590/235‑1 (MQ=255)
                                                                      accaccATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGaa                                                                                                                                                                                                                             >  2:412046/1‑170 (MQ=255)
                                                                      accaccATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGaa                                                                                                                                                                                                                             <  1:412046/170‑1 (MQ=255)
                                                                           caTCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAACATATTCGGATTTAAATTGATGCTCTTTCGGACCGTCTGGGCTGTc                                                                                                                                                      >  2:147548/1‑236 (MQ=255)
                                                                           caTCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTc                                                                                                                                                      >  1:181422/1‑236 (MQ=255)
                                                                                     cAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGAt                                                                                                                                               >  1:724598/1‑233 (MQ=255)
                                                                                       gACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGc                                                                                                                                          <  1:530427/236‑1 (MQ=255)
                                                                                       gACCTCGCTCAATGAGCAAATCGCATGTCACTACATTTTGAGGTGAGACGCCGACCTGCTGATTAACGTGGTGGTTGCGTCTAACCTTGCGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGc                                                                                                                                          <  2:265907/236‑1 (MQ=255)
                                                                                        aCCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGCGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGTGGCGCCTGGGCGGTCCGCGGATCCCGCt                                                                                                                                         >  2:80814/1‑236 (MQ=255)
                                                                                                  aTGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCG‑‑GCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAAccc                                                                                                                              >  2:588911/1‑235 (MQ=255)
                                                                                                         aaTCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGt                                                                                                                         <  1:714658/235‑1 (MQ=255)
                                                                                                         aaTCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGt                                                                                                                         <  1:147548/235‑1 (MQ=255)
                                                                                                          aTCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTggg                                                                                                                                                           <  2:583680/200‑1 (MQ=255)
                                                                                                          aTCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTggg                                                                                                                                                           >  1:583680/1‑200 (MQ=255)
                                                                                                           tCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTcg                                                                                                                       <  1:640447/235‑1 (MQ=255)
                                                                                                           tCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTcg                                                                                                                       <  1:522268/235‑1 (MQ=255)
                                                                                                               cTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGTTCGCggaa                                                                                                                  >  2:743551/1‑234 (MQ=255)
                                                                                                                  tCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTg                                                                                                               <  1:617004/236‑1 (MQ=255)
                                                                                                                        cATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCACGGTATTGAAgcgc                                                                                                         >  2:12422/1‑236 (MQ=255)
                                                                                                                             tGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCTCGGTATTGATgcgcg                                                                                                        >  1:290470/1‑231 (MQ=255)
                                                                                                                                          cGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGAt                                                                                        <  1:80814/235‑1 (MQ=255)
                                                                                                                                                gctgATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCtata                                                                                 <  2:724598/236‑1 (MQ=255)
                                                                                                                                                gctgATTAACGTGATGGATGCATCTACCCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCtata                                                                                 <  2:152124/236‑1 (MQ=255)
                                                                                                                                                      tAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTa                                                                           >  1:500845/1‑236 (MQ=255)
                                                                                                                                                        aCGGGGTGGAGTCGTCTAACCTTGAGCGAAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAAc                                                                         <  2:181422/236‑1 (MQ=255)
                                                                                                                                                              tggATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGc                                                                                                                                          >  2:605730/1‑165 (MQ=255)
                                                                                                                                                              tggATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGc                                                                                                                                          <  1:605730/165‑1 (MQ=255)
                                                                                                                                                                gATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAAtgt                                                                 >  2:16673/1‑236 (MQ=255)
                                                                                                                                                                        tAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCgg                                                                                                                                                                     <  2:760333/128‑1 (MQ=255)
                                                                                                                                                                        tAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCgg                                                                                                                                                                     >  1:760333/1‑128 (MQ=255)
                                                                                                                                                                        tAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACt                                                           >  1:740531/1‑234 (MQ=255)
                                                                                                                                                                               gAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCgg                                                                                                                                                                     >  2:88566/1‑121 (MQ=255)
                                                                                                                                                                               gAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCgg                                                                                                                                                                     <  1:88566/121‑1 (MQ=255)
                                                                                                                                                                                aGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACTGTGGCATTaa                                                 >  2:342348/1‑235 (MQ=255)
                                                                                                                                                                                        tggTACCAGACGCTACAACTGCTGGAACTGGGCATTCCCTGAATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACTGGTGCATTACGCACAGc                                          <  2:500845/233‑1 (MQ=255)
                                                                                                                                                                                        cTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACTGGTGCATTACGCACAGc                                          <  1:12422/235‑1 (MQ=255)
                                                                                                                                                                                        cTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACTGGTGCATTACGCACAGc                                          <  1:265089/235‑1 (MQ=255)
                                                                                                                                                                                          gTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACTGGTGCATTACGcaca                                            >  2:298169/1‑231 (MQ=255)
                                                                                                                                                                                            aCCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACTGGTGCATTACGCACAGCCgctg                                     <  1:514502/236‑1 (MQ=255)
                                                                                                                                                                                                 aCGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGGGAATGTGGAACTGGTGCATTACGCACAGCCGCTGGTCaa                                >  1:179366/1‑236 (MQ=255)
                                                                                                                                                                                                 aCGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACTGGTGCATTACGCACAGCCGCTGCTCaa                                >  2:209941/1‑236 (MQ=255)
                                                                                                                                                                                                  cGCTACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACTGGTGCATTACGCACAGCCGCTGCTCAAc                               <  1:342348/236‑1 (MQ=255)
                                                                                                                                                                                                     tACAACTGCTGGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACTGGGGCATTACGCACAGCCGCTGCTCAACGaa                            >  1:201348/1‑236 (MQ=255)
                                                                                                                                                                                                             ctgGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTggg                                                                                                                                                           <  2:234136/101‑1 (MQ=255)
                                                                                                                                                                                                             ctgGAACTCGGCATTCCCTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTggg                                                                                                                                                           >  1:234136/1‑101 (MQ=255)
                                                                                                                                                                                                                             ccTGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACTGGTGCATTACGCACAGCCGCTGCTCAACGAAGCAGATTCACTGGCAAAAGTGATg    <  1:298169/236‑1 (MQ=255)
                                                                                                                                                                                                                               tGCATGGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTGCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACTGGTGCATTACGCACAGCCGCTGCTCAACGAAGCAGATTCACTGGCAAAAGTGATGcc  <  2:151600/236‑1 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      
TAAAGAAGGGCAATTCTCCACCACCGATCATCAGGTCACGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGCTATAAAGCTAACGAGAATGTGGAACTGGTGCATTACGCACAGCCGCTGCTCAACGAAGCAGATTCACTGGCAAAAGTGATGCC  >  NC_000913/3540526‑3540984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: