Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,623,709	T→G	I104S (ATT→AGT)	yhhH →	putative NTF2 fold immunity protein for polymorphic toxin RhsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,623,709	0	T	G	100.0%	230.4 / NA	62	I104S (ATT→AGT)	yhhH	putative NTF2 fold immunity protein for polymorphic toxin RhsB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/33);  total (29/33)

GGGCGCAGTGTAATAATAATATTAAAATAATGCGCAAGTATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTA  >  NC_000913/3623487‑3623934                                                                                                                                                                                                                               |                                                                                                                                                                                                                                  gggCGCAGTGTAATAATAATATTAAAATAATGCGCAAGTATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATAACTGAGTTAACAACAAGTTGGGTTGTTGAAGGAACCATTAACTCAGACCAAAGTGCTGGTgggggt                                                                                                                                                                                                                      >  2:124986/1‑234 (MQ=255)                   atatTAAAATAATGCGCAAGTATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGCCCAAAGAGCTGGTGGGGTTTTTATTATAGCAATCGGc                                                                                                                                                                                                    >  2:732926/1‑236 (MQ=255)                   atatTAAAATAATGCGCAAGTATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAAt                                                                                                                                                                                                        <  1:687508/232‑1 (MQ=255)                   atatTAAAATAATGCGCAAGTATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAAt                                                                                                                                                                                                        >  2:687508/1‑232 (MQ=255)                               tGCGCAAGTATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTttat                                                                                                                                                                                                                 <  2:722158/211‑1 (MQ=255)                               tGCGCAAGTATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTttat                                                                                                                                                                                                                 >  1:722158/1‑211 (MQ=255)                               tGCGCAAGTATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATggg                                                                                                                                                                                        >  2:434178/1‑236 (MQ=255)                                                taagtttaaTATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTgg                                                                                                                                                                       <  2:404594/229‑1 (MQ=255)                                                 aaGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGc                                                                                                                                                                      >  1:234879/1‑236 (MQ=255)                                                 aaGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGc                                                                                                                                                                      >  1:626798/1‑236 (MQ=255)                                                              cGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCa                                                                                                                                                                    >  1:473105/1‑225 (MQ=255)                                                              cGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAACTAAg                                                                                                                                                         >  2:146857/1‑236 (MQ=255)                                                              cGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGACATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCa                                                                                                                                                                    <  2:473105/225‑1 (MQ=255)                                                               gTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATaa                                                                                                                                                          >  1:274728/1‑234 (MQ=255)                                                               gTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATaa                                                                                                                                                          <  2:274728/234‑1 (MQ=255)                                                                ttAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTa                                                                                                                                                       >  1:435322/1‑236 (MQ=255)                                                                        ttGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGGAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGa                                                                                                                                               <  1:124986/236‑1 (MQ=255)                                                                        ttGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAg                                                                                                                                                 <  2:234879/234‑1 (MQ=255)                                                                        ttGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGa                                                                                                                                               >  2:305182/1‑236 (MQ=255)                                                                        ttGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGa                                                                                                                                               >  1:39154/1‑236 (MQ=255)                                                                                  aTAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACa                                                                                                                                     <  2:435322/236‑1 (MQ=255)                                                                                            aGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTgcgc                                                                                                                           >  2:399949/1‑236 (MQ=255)                                                                                                  ggAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATgg                                                                                                                     >  1:760649/1‑236 (MQ=255)                                                                                                  ggAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATgg                                                                                                                     >  1:275156/1‑236 (MQ=255)                                                                                                   gAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGc                                                                                                                    >  2:184242/1‑236 (MQ=255)                                                                                                   gAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGc                                                                                                                    >  2:726504/1‑236 (MQ=255)                                                                                                             agaTATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGaaa                                                                                                                                                                                                         >  1:713280/1‑141 (MQ=255)                                                                                                             agaTATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTAGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGaaa                                                                                                                                                                                                         <  2:713280/141‑1 (MQ=255)                                                                                                               atatatGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTgga                                                                                                        <  2:760649/236‑1 (MQ=255)                                                                                                               atatatGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTgga                                                                                                        <  1:399949/236‑1 (MQ=255)                                                                                                                       tAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCa                                                                                                <  1:399352/236‑1 (MQ=255)                                                                                                                          gAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGa                                                                                              <  1:726504/235‑1 (MQ=255)                                                                                                                              cGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGa                                                                                                                                               <  2:168073/182‑1 (MQ=255)                                                                                                                              cGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGa                                                                                                                                               >  1:168073/1‑182 (MQ=255)                                                                                                                                ttATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCaa                                                                                       >  1:83400/1‑236 (MQ=255)                                                                                                                                ttATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCaa                                                                                       >  1:219629/1‑236 (MQ=255)                                                                                                                                              ccGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGa                                                                                   <  2:39154/226‑1 (MQ=255)                                                                                                                                               cGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCt                                                                                                                          <  1:81200/186‑1 (MQ=255)                                                                                                                                               cGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCt                                                                                                                          >  2:81200/1‑186 (MQ=255)                                                                                                                                               cGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGa                                                                         <  2:626798/235‑1 (MQ=255)                                                                                                                                               cGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGa                                                                         >  2:412380/1‑235 (MQ=255)                                                                                                                                               cGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGAt                                                                        <  1:146857/236‑1 (MQ=255)                                                                                                                                               cGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGAt                                                                        <  2:219629/236‑1 (MQ=255)                                                                                                                                               cGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGAt                                                                        <  2:275156/236‑1 (MQ=255)                                                                                                                                                    aagaagaaAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCt                                                                   >  1:553941/1‑236 (MQ=255)                                                                                                                                                           aaaaCCATCCGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGa                                                            <  2:472399/236‑1 (MQ=255)                                                                                                                                                           aaaaCCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATg                                                              <  1:732926/234‑1 (MQ=255)                                                                                                                                                           aaaaCCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGa                                                            <  1:434178/236‑1 (MQ=255)                                                                                                                                                              aCCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAACCTTTATGGAGGt                                                         >  2:756991/1‑236 (MQ=255)                                                                                                                                                                     gAAATCAATGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCCGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCa                                                  <  1:305182/236‑1 (MQ=255)                                                                                                                                                                          cACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGt                                              <  2:674839/235‑1 (MQ=255)                                                                                                                                                                              gAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGTCAAat                                         >  2:221852/1‑236 (MQ=255)                                                                                                                                                                                   aacaacAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGTCAAatacata                                    >  1:710810/1‑236 (MQ=255)                                                                                                                                                                                      aacaaGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGTCAAATACATACCa                                 <  1:184242/236‑1 (MQ=255)                                                                                                                                                                                                 ttgttgAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAgg                      <  2:710810/236‑1 (MQ=255)                                                                                                                                                                                                 ttgttgAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAgg                      <  2:83400/236‑1 (MQ=255)                                                                                                                                                                                                  tgttgAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGc                     <  2:553941/236‑1 (MQ=255)                                                                                                                                                                                                      gAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGt                 >  2:258869/1‑236 (MQ=255)                                                                                                                                                                                                       aaGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                <  1:221852/236‑1 (MQ=255)                                                                                                                                                                                                               aTTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt          <  1:719812/234‑1 (MQ=255)                                                                                                                                                                                                                  cACTCAGACCAAAGTGCTTGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGt     <  2:132057/236‑1 (MQ=255)                                                                                                                                                                                                                     tCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTta  <  2:757412/236‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                  GGGCGCAGTGTAATAATAATATTAAAATAATGCGCAAGTATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTA  >  NC_000913/3623487‑3623934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑