Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,697,854	A→C	Q466P (CAA→CCA)	bcsE →	cellulose production protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,697,854	0	A	C	100.0%	243.6 / NA	70	Q466P (CAA→CCA)	bcsE	cellulose production protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/34);  total (36/34)

TCTGGTTGCCCTACGTCCGGTACCGGGTATCCGCGTTGAACAAGCCCTGACGCTGTGTCGCCCTAACCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTT  >  NC_000913/3697633‑3698079                                                                                                                                                                                                                              |                                                                                                                                                                                                                                    tCTGGTTGCCCTACGTCCGGTACCGGGTATCCGCGTTGAACAAGCCCTGACGCTGTGTCGCCCTAACCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAg                                                                                                                                                                                                                       <  1:124295/235‑1 (MQ=255) tCTGGTTGCCCTACGTCCGGTACCGGGTATCCGCGTTGAACAAGCCCTGACGCTGTGTCGCCCTAACCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCTGGGGCTGTTTTTCTCCTTTTGTCGGGTTAACGATCTTGATACCGCGGTTAATCATATTTTTCCATTTCCTACTGGCGACATTTTCTCAAACCGCACGGGTTGGGTTGTAGATGATCCAAACacagtc                                                                                                                                                                                                                           >  2:469901/1‑227 (MQ=255)  cTGGTTGCCCTACGTCCGGTACCGGGTATCCGCGTTGAACAAGCCCTGACGCTGTGTCGCCCTAACCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGCCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCt                                                                                                                                                                                                                     >  1:475723/1‑236 (MQ=255)                     tACCGGGTATCCGCGTTGAACAAGCCCTGACGCTGTGTCGCCCTAACCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCCGGGGCTGTTTCTCCCATTCTGTCGGGTTAACGATTTGGGTACCGCGGTGAATCATATTTTCCCATTGCCTACCGGGGACATTTTCTTAAAACGCATGGGCTGGGTTTAAGAAGAGCCAATACA‑TGGCCAGCTGGGGCAGCTGCgattgac                                                                                                                                                                                                   >  2:673243/1‑233 (MQ=255)                     tACCGGGTAACCGCGTTGACCGAGCCCTGACGCTGTGTCGCCCTAACCGTAACGGCGATATCATGACCATTGGCGGTAATCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCgcttgct                                                                                                                                                                                                   <  2:289539/235‑1 (MQ=255)                          ggTATCCGCGTTGAACAAGCCCTGACGCTGTGTCGCCCTAACCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCTGGGGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCCAAACGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGcccc                                                                                                                                                                                             >  1:668878/1‑236 (MQ=255)                           gTATCCGCGTTGAACAAGCCCTGACGCTGTGTCGCCCTAACCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCa                                                                                                                                                                                            <  1:469901/236‑1 (MQ=255)                            tATCCGCGTTGAACAAGCCCTGACGCTGTGTCGCCCTAACCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAg                                                                                                                                                                                           <  2:235334/236‑1 (MQ=255)                              tCCGCGTTGAACAAGCCCTGACGCTGTGTCGCCCTAACCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGccc                                                                                                                                                                                              <  1:748598/231‑1 (MQ=255)                                                        tgtCGCCCTAACCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCCGAACAATAGGGCATGCCGCTGCCCTTA‑Ac                                                                                                                                                              >  1:175450/1‑236 (MQ=255)                                                           cGCCCTAACCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCa                                                                                                                                                           <  1:311867/236‑1 (MQ=255)                                                                  aCCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTc                                                                                                                                                     >  1:496515/1‑235 (MQ=255)                                                                  aCCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCTGGGGCTGTTTCTCTCATTTTTTCGGGTTTACGATCTGGCTACCGCGTTGAATCATATTTTTCCCTTGCCTACTTGCGACATTTTCTCAAACCGTATGGTCTTGGTTGAAGATGATCCAAACAGCTTCGGAGCTGG‑GCAGATGCGCTTGCTTGCTCCCGAACAAATGTGCATGCCGCTGCCCTTACAGGCAAAGtc                                                                                                                                                      >  2:654541/1‑234 (MQ=255)                                                                     ggacctgtgATATCGTGGCCATGGGCGGTAATCTGCTGGTGCTGTTTCTCTCATTCTGTCTGATTAACGATCTGGATTCCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTaaa                                                                                                                                                 <  2:166727/228‑1 (MQ=255)                                                                        ccGGCGATATCATGACCATTGGCGGTAATCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTTTAAACCg                                                                                                                                              >  1:264551/1‑236 (MQ=255)                                                                        ccGGAGATTTCATGACCATGGGCCGTTAGCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCg                                                                                                                                              <  2:472067/236‑1 (MQ=255)                                                                             gATAACATCACCATTGGCGGTAATCGGCTGGTGCTGTTGCTCACATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTACCATTGCCTACGGGCGACATGTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGtcat                                                                                                                                         <  1:95267/236‑1 (MQ=255)                                                                                   aTGACCATGGGCGGTACTCGGCTGGTGCTGGTTCTCTAATTCGGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGc                                                                                                                                   <  1:654541/236‑1 (MQ=255)                                                                                    tGACCATTGGCGGTAATCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGcc                                                                                                                                  <  2:496515/236‑1 (MQ=255)                                                                                           tGGCGGTAATCGGCTGGTGCTGTTTCTCTCCTTCTGTCGGATTAACGATCTGGATACCGCGTTGGATCATATTTTCCCCTTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATACAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAAACATTGGGCATGCCCCTGCCCTTT‑ACCCCAAGTTCTAAAACGGTCATCAATGTCAAGCAct                                                                                                                           >  2:30414/1‑235 (MQ=255)                                                                                                         tGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGAATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCACTGCCGAGCACGATGGTCGCCACTg                                                                                                              >  2:691057/1‑235 (MQ=255)                                                                                                            tGCTGTTTCTTCTAGTCTGGCGGATTAACGATCTGGATACCGCGTTGATTCATCTTTGCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcg                                                                                                           <  2:426651/235‑1 (MQ=255)                                                                                                               tGTTGCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcgacg                                                                                                        <  1:743956/235‑1 (MQ=255)                                                                                                                gTTTCCATCAGTCTGGCGGATTTACGATCTGGATACCGCTTTTAATCCTATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCCGATGGTCTGGTTTGAAGATGATCCAATCAG‑GGCCGAGCTGGTGCCGATGCGCTTTCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcgacga                                                                                                       <  2:175450/235‑1 (MQ=255)                                                                                                                gTTGCTCTCATTCTGTCGGATTAACGATCTTGATTCCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTTGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcgacga                                                                                                       <  1:673243/235‑1 (MQ=255)                                                                                                                 tttCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAAt                                                                                                     <  1:469358/236‑1 (MQ=255)                                                                                                                      ctcATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCa                                                                                                 >  1:481563/1‑235 (MQ=255)                                                                                                                      ctcATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAAACGGTCATCAATGCCGAGCCACATGGTCGCCACTGGCGACGAATACCa                                                                                                 >  2:743956/1‑235 (MQ=255)                                                                                                                           tCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcgacg                                                                                                        <  2:398583/223‑1 (MQ=255)                                                                                                                           tCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcgacg                                                                                                        >  1:398583/1‑223 (MQ=255)                                                                                                                               tCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCAt                                                                                         >  1:253196/1‑234 (MQ=255)                                                                                                                                 ggATTAACGATTCGGATTCCGCGTTGAATCATATTTTCCCATTGCCTACCGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGa                                                                                     <  2:475723/236‑1 (MQ=255)                                                                                                                                   aTTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACACAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACt                                                                                   >  2:290379/1‑236 (MQ=255)                                                                                                                                         gATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACACAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAga                                                                             <  1:290379/236‑1 (MQ=255)                                                                                                                                           tCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAgatg                                                                           <  1:16603/236‑1 (MQ=255)                                                                                                                                            cTGGATCCCGAGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAgatga                                                                          <  2:264551/236‑1 (MQ=255)                                                                                                                                                   ccGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACa                                                                                                                                                                                       <  2:541400/121‑1 (MQ=255)                                                                                                                                                   ccGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACa                                                                                                                                                                                       >  1:541400/1‑121 (MQ=255)                                                                                                                                                    cgcgTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACCGTTAGATGATGCTGTgg                                                                  >  1:89477/1‑236 (MQ=255)                                                                                                                                                      cgTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCt                                                                      >  1:378770/1‑230 (MQ=255)                                                                                                                                                      cgTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGa                                                                 >  2:167440/1‑235 (MQ=255)                                                                                                                                                      cgTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTATGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCt                                                                      <  2:378770/230‑1 (MQ=255)                                                                                                                                                        ttGAATCATATTTGCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAgcg                                                              <  1:542173/236‑1 (MQ=255)                                                                                                                                                         tGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGCCTGTTAGATGATGCTGTGGAgcgc                                                             >  1:502016/1‑236 (MQ=255)                                                                                                                                                         tGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGCTGATGCTGTGGAgcgc                                                             >  1:460602/1‑236 (MQ=255)                                                                                                                                                         tGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAgcgc                                                             >  1:166632/1‑236 (MQ=255)                                                                                                                                                               atatTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCtcatca                                                       <  1:30414/236‑1 (MQ=255)                                                                                                                                                                            gCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAg                                          >  2:119366/1‑236 (MQ=255)                                                                                                                                                                                  tGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGatat                                    >  1:639165/1‑236 (MQ=255)                                                                                                                                                                                       aCATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGACGGCTCATCATGATGACCATCAGCGATAGCATTg                               >  2:20552/1‑236 (MQ=255)                                                                                                                                                                                              ctcAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGAcc                                               <  1:362017/213‑1 (MQ=255)                                                                                                                                                                                              ctcAAACCGTATGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGAcc                                               >  2:362017/1‑213 (MQ=255)                                                                                                                                                                                                        aTGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTgc              <  1:20552/236‑1 (MQ=255)                                                                                                                                                                                                         tGGTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTgcg             <  2:166632/236‑1 (MQ=255)                                                                                                                                                                                                           gTCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCa           <  1:525640/236‑1 (MQ=255)                                                                                                                                                                                                            tCTGGTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCa           <  1:119366/235‑1 (MQ=255)                                                                                                                                                                                                             cTGGTTTGAAGTGGATCCAACCAG‑TGCCGAGCTGGTGAAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTGTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACt         <  2:726675/236‑1 (MQ=255)                                                                                                                                                                                                               ggTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGa                                       >  2:443458/1‑204 (MQ=255)                                                                                                                                                                                                               ggTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGa                                       <  1:443458/204‑1 (MQ=255)                                                                                                                                                                                                               ggTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAatta                         >  2:81807/1‑218 (MQ=255)                                                                                                                                                                                                               ggTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAatta                         <  1:81807/218‑1 (MQ=255)                                                                                                                                                                                                               ggTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGa       >  1:726675/1‑236 (MQ=255)                                                                                                                                                                                                               ggTTTGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGa       >  1:216578/1‑236 (MQ=255)                                                                                                                                                                                                                 tttGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGGGCTCATCATGATGACCATCAGCGATATCATTGCACTTATTGTCGTTTGCGCACTGaga     >  2:544274/1‑234 (MQ=255)                                                                                                                                                                                                                 tttGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGata     >  2:277235/1‑236 (MQ=255)                                                                                                                                                                                                                   tGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGa                              >  2:501663/1‑209 (MQ=255)                                                                                                                                                                                                                   tGAAGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGa                              <  1:501663/209‑1 (MQ=255)                                                                                                                                                                                                                     aaGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATAttt  >  1:664962/1‑235 (MQ=255)                                                                                                                                                                                                                     aaGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGGGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGACCGGATAttt  >  2:412066/1‑235 (MQ=255)                                                                                                                                                                                                                     aaGATGATCCAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCACAACCCATGCGACTGTTAGATGATGCTGTGGATCGCTCATCAGGATGACCATCAGCGATATCATTGCAATTATTGTCGTCTGCGCACTGATAttt  >  2:532413/1‑235 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                    TCTGGTTGCCCTACGTCCGGTACCGGGTATCCGCGTTGAACAAGCCCTGACGCTGTGTCGCCCTAACCGTACCGGCGATATCATGACCATTGGCGGTAATCGGCTGGTGCTGTTTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAG‑TGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTA‑ACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTT  >  NC_000913/3697633‑3698079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑