Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,710,019	T→G	T153P (ACC→CCC)	eptB ←	KDO phosphoethanolamine transferase, Ca(2+)‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,710,019	0	T	G	100.0%	97.2 / NA	29	T153P (ACC→CCC)	eptB	KDO phosphoethanolamine transferase, Ca(2+)‑inducible
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/0);  total (29/0)

TGAATTATTATCGGAAGATTCATCGACCCGCGCCCAGGCATACAGCCCCAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAG  >  NC_000913/3709789‑3710247                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     tGAATTATTATCGGAAGATTCATCGACCCGCGCCCAGGCATACAGCCCCAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGTGGGTCGCa                                                                                                                                                                                                                                 >  2:462806/1‑236 (MQ=255) tGAATTATTATCGGAAGATTCATCGACCCGCGCCCAGGCATACAGCCCCAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCa                                                                                                                                                                                                                                 >  1:570743/1‑236 (MQ=255)  gAATTATTATCGGAAGATTCATCGACCCGCGCCCAGGCATACAGCCCCAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTc                                                                                                                                                                                                                                    >  1:147711/1‑232 (MQ=255)             ggAAGATTCATCGACCCGCGCCCAGGCATACAGCCCCAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTCGGGGTCGCCGTTGTTGGAGCa                                                                                                                                                                                                                     >  1:356443/1‑236 (MQ=255)                                                ccAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTCATTCCAgataaggata                                                                                                                                                                                  >  1:636646/1‑236 (MQ=255)                                                ccAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTCGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTGCTTCCAgataaggata                                                                                                                                                                                  >  2:392592/1‑236 (MQ=255)                                                          aaGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCaa                                                                                                                                                                          >  1:486491/1‑234 (MQ=255)                                                                       ggCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGc                                                                                                                                                                                                      >  1:469066/1‑193 (MQ=255)                                                                                      gCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATaa                                                                                                                                             >  1:351353/1‑235 (MQ=255)                                                                                       cTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATCAAg                                                                                                                                           >  1:368209/1‑236 (MQ=255)                                                                                                 gCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGGGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATACAGTTCAGACca                                                                                                                                  >  2:706298/1‑235 (MQ=255)                                                                                                            gCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGCCAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCtttt                                                                                                                      >  2:583298/1‑236 (MQ=255)                                                                                                            gCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACGGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCtttt                                                                                                                      >  1:728613/1‑236 (MQ=255)                                                                                                            gCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCtttt                                                                                                                      >  1:635560/1‑236 (MQ=255)                                                                                                                          gTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATc                                                                                                        >  2:136473/1‑236 (MQ=255)                                                                                                                                             tctGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAAACACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCg                                                                                     >  1:665830/1‑236 (MQ=255)                                                                                                                                                         ccAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCCGATAAGGATAAGNNNCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTGTTGGCAGGTCGATAACGGTGGGCATGACGGAAGCACTGATACCAtca                                                                         >  2:692179/1‑234 (MQ=255)                                                                                                                                                                                          ccGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCgatg                                                                                  >  1:159324/1‑194 (MQ=255)                                                                                                                                                                                          ccGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGCCATGACGGCAGCGATAATGCCATAACCCATGACCACCTTCAGGAAGGGCATGTatatg                                        >  2:120814/1‑232 (MQ=255)                                                                                                                                                                                            gCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCt                                      >  1:643217/1‑236 (MQ=255)                                                                                                                                                                                                 tACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCTg                                     >  1:374674/1‑232 (MQ=255)                                                                                                                                                                                                 tACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGGCATGACTGAAGCGAGAATGCCATAACCAATGACACCCTCAAGGAAGGGCATGTGATAACTgcgca                                 >  2:426749/1‑232 (MQ=255)                                                                                                                                                                                                      cgacCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCaga                                                                                                                                                   >  2:228896/1‑117 (MQ=255)                                                                                                                                                                                                      cgacCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCTGGCACCTGCg                            >  2:178151/1‑236 (MQ=255)                                                                                                                                                                                                      cgacCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCCGGCaacgtct                            >  2:390324/1‑230 (MQ=255)                                                                                                                                                                                                        acCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGAGGATGCCATAACCAATGACCACATTAAGGAAGGGCATGTACTACCTGGGACCTGCGGa                          >  2:505410/1‑236 (MQ=255)                                                                                                                                                                                                                          cGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGa        >  1:204274/1‑236 (MQ=255)                                                                                                                                                                                                                              gCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACcaac                                                                                                                                >  2:296644/1‑112 (MQ=255)                                                                                                                                                                                                                                ccTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAg  >  2:758112/1‑236 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     TGAATTATTATCGGAAGATTCATCGACCCGCGCCCAGGCATACAGCCCCAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAG  >  NC_000913/3709789‑3710247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑