Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,752,058	A→C	I23L (ATC→CTC)	yiaU →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,752,058	0	A	C	100.0%	182.1 / NA	51	I23L (ATC→CTC)	yiaU	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/25);  total (26/25)

GCAAATAACGCCACAATATTGCGATTAATTAACATGTCCGGTATTCCATTTTAAAATAAGATAAAAAAGAGTCGGCATAATAATGCTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACATCAACGACATGCTGGACAGAAACGAGCAACTTAATAATTTTATTGCAGATTATAAGCATGAGAAACGTGGTCGGGTCA  >  NC_000913/3751834‑3752286                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                     gCAAATAACGCCACAATATTGCGATTAATTAACATGTCCGGTATTCCATTTTAAAATAAGATAAAAAAGAGTCGGCATAATAATGCTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGc                                                                                                                                                                                                                            <  2:11318/235‑1 (MQ=255)     aTAACGCCACAATATTGCGATTAATTAACATGTCCGGTATTCCATTTTAAAATAAGATAAAAAAGAGTCGGCATAATAATGCTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGcc                                                                                                                                                                                                                           >  1:741938/1‑232 (MQ=255)            cacaATATTGCTATTAATTAACATGTCCGGTATTCCATTTTAAAATAAGATAAAAAAGAATCGGCATAATAATGCTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACt                                                                                                                                                                                                                <  2:744362/236‑1 (MQ=255)                     gCGATTAATTAACATGTCCGGTATTCCATTTTAAAATAAGATAAAAAAGAGTCGGAATAATAATGCTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCa                                                                                                                                                                                                           <  1:261844/232‑1 (MQ=255)                      caattaattaACATGTCCTGTATTCCATTTTAAAATAAAATAAAAAATATTCGGCATAATAATGCTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGca                                                                                                                                                                                                      <  2:741938/234‑1 (MQ=255)                         ttaattaaCATGTCCGGTATTCCATTTTAAAATAAGATAAAAAAGAGTCGGCATAATAATGCTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGAACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCTCGACTGTACTTGGGTTCGCACAg                                                                                                                                                                                                   >  2:265333/1‑236 (MQ=255)                                        ggTATTCCATTTTAAAATAAGATAAAAAAGAGTCGGCATAATAATGCTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCa                                                                                                                                                                                         >  2:278194/1‑231 (MQ=255)                                               cATTTTAAAATAAGATAAAAAAGAGTCGGCATAATAATGCTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGca                                                                                                                                                                                                      >  1:489901/1‑211 (MQ=255)                                               cATTTTAAAATAAGATAAAAAAGAGTCGGCATAATAATGCTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGca                                                                                                                                                                                                      <  2:489901/211‑1 (MQ=255)                                                    tAAAATAAGATAAAAAAGAGTCGGCATAATAATGCTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGTTGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCATTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCACTGACCAGGCCACGGTCAGCAAATATCTTCCTGac                                                                                                                                                                        >  2:496990/1‑235 (MQ=255)                                                                            cATAATAATGCTTACAATTTCTCCGGTTCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTaaa                                                                                                                                                 <  2:573396/235‑1 (MQ=255)                                                                                      cTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACt                                                                                                                                                                                                                     >  1:422383/1‑157 (MQ=255)                                                                                      cTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACt                                                                                                                                                                                                                     <  2:422383/157‑1 (MQ=255)                                                                                                ctcCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGt                                                                                                                                                          >  1:277248/1‑206 (MQ=255)                                                                                                ctcCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGt                                                                                                                                                          <  2:277248/206‑1 (MQ=255)                                                                                                                atatTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAAt                                                                                                                                                                 >  1:359871/1‑183 (MQ=255)                                                                                                                atatTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAAt                                                                                                                                                                 <  2:359871/183‑1 (MQ=255)                                                                                                                           gTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCAc                                                                                                 <  1:102055/236‑1 (MQ=255)                                                                                                                                           tatataATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCAc                                                                                                 >  2:302349/1‑220 (MQ=255)                                                                                                                                           tatataATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCAc                                                                                                 <  1:302349/220‑1 (MQ=255)                                                                                                                                           tatataATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTAc                                                                                 >  1:681757/1‑236 (MQ=255)                                                                                                                                                  ttGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAac                                                                                                                                  <  1:691158/180‑1 (MQ=255)                                                                                                                                                  ttGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAac                                                                                                                                  >  2:691158/1‑180 (MQ=255)                                                                                                                                                     ggCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGAAGCTCACACCTTTTGGCACCGCTCTGTTACCGTACATCAACGCCa                                                                       >  2:116785/1‑236 (MQ=255)                                                                                                                                                          ttGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATTGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCGGTTACCGTACATCAACGACATGCTg                                                                  >  2:700589/1‑236 (MQ=255)                                                                                                                                                          ttGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTcaca                                                                                                            <  2:375111/194‑1 (MQ=255)                                                                                                                                                          ttGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTcaca                                                                                                            >  1:375111/1‑194 (MQ=255)                                                                                                                                                          ttGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACATCAACGACATGCTg                                                                  >  1:592644/1‑236 (MQ=255)                                                                                                                                                                       aTTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACATCAACGACATGCTGGACAGAAACGAGc                                                     <  1:116785/236‑1 (MQ=255)                                                                                                                                                                               ttAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACATCAACGACATGCTGGACAGAAACGAGCAACTtaat                                             <  2:592644/236‑1 (MQ=255)                                                                                                                                                                                tAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCAc                                                                                                 <  2:78049/183‑1 (MQ=255)                                                                                                                                                                                tAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCAc                                                                                                 >  1:78049/1‑183 (MQ=255)                                                                                                                                                                                         ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGt                                                                                                                                                          >  2:323201/1‑117 (MQ=255)                                                                                                                                                                                         ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGt                                                                                                                                                          <  1:323201/117‑1 (MQ=255)                                                                                                                                                                                         ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTgg                                                                                                                                                        <  2:574775/119‑1 (MQ=255)                                                                                                                                                                                         ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTgg                                                                                                                                                        >  1:574775/1‑119 (MQ=255)                                                                                                                                                                                         ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTaaa                                                                                                                                                 <  2:190653/126‑1 (MQ=255)                                                                                                                                                                                         ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTaaa                                                                                                                                                 >  1:190653/1‑126 (MQ=255)                                                                                                                                                                                         ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAg                                                                                                                        <  2:22921/151‑1 (MQ=255)                                                                                                                                                                                         ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAg                                                                                                                        >  1:22921/1‑151 (MQ=255)                                                                                                                                                                                         ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTcaca                                                                                                            >  1:275869/1‑163 (MQ=255)                                                                                                                                                                                         ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTcaca                                                                                                            <  2:275869/163‑1 (MQ=255)                                                                                                                                                                                         ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACATCAACGACATGCTGGACAGAAACGAGCAACTTAATAAtttt                                       >  1:743426/1‑232 (MQ=255)                                                                                                                                                                                         ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACATCAACGACATGCTGGACAGAAACGAGCAACTTAATAATTTTATTg                                   >  1:573694/1‑236 (MQ=255)                                                                                                                                                                                             ttAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACATCAACGACATGCTGGACAGAAACGAGCAACTTAATAATTTTATTg                                   <  2:681757/232‑1 (MQ=255)                                                                                                                                                                                                      aTCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGca                                                                                                                                                                                                      >  2:148422/1‑60 (MQ=255)                                                                                                                                                                                                      aTCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGca                                                                                                                                                                                                      <  1:148422/60‑1 (MQ=255)                                                                                                                                                                                                            gTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGt                                                                                                                                                          <  1:155350/98‑1 (MQ=255)                                                                                                                                                                                                            gTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGt                                                                                                                                                          >  2:155350/1‑98 (MQ=255)                                                                                                                                                                                                                     gCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACATCAACGACATGCTGGACAGAAACGAGCAACTTAATAATTTTATTGCAGATTATAACCATAGGAAACGTGGTCg       >  1:214515/1‑236 (MQ=255)                                                                                                                                                                                                                              aaTCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACATCAACGACATGCTGGACAGAAACGAGCAACTTAATAATTTTATTGCAGATTATAAGCATGAGAAACGTGGTCGGGTCa  <  1:278194/232‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                     GCAAATAACGCCACAATATTGCGATTAATTAACATGTCCGGTATTCCATTTTAAAATAAGATAAAAAAGAGTCGGCATAATAATGCTTACAATTTCTCCGGTGCAAGATTCATATTTATATAGTAATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACATCAACGACATGCTGGACAGAAACGAGCAACTTAATAATTTTATTGCAGATTATAAGCATGAGAAACGTGGTCGGGTCA  >  NC_000913/3751834‑3752286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑