Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,790,919 A→C L143V (TTA→GTA)  tdh ← L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,790,9190AC100.0% 202.1 / NA 60L143V (TTA→GTA) tdhL‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (33/27);  total (33/27)

AGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCA  >  NC_000913/3790687‑3791145
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                  
agaTTTTCTTTGCCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCAGACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGACGGGATCACAAATTGAGGCTACGTc                                                                                                                                                                                                                                 >  2:416494/1‑236 (MQ=255)
      tCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCCAAAATTGTGGCTACGTCATCggg                                                                                                                                                                                                                           >  2:689082/1‑235 (MQ=255)
                 gTTAACCGACCGGGTGATCCCCTTTTTCCCCGCCAGCTCAAGGCGGTATTCGTTAACATCAGCGATCACCACATTGCGCGCCCCAACGTTTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCg                                                                                                                                                                                                                 <  1:714823/235‑1 (MQ=255)
                   tAACCGCACGGGTTATACCCATTTTACGCGACAGCTCAAGGCGTTATTCGTTAACATCAGTGATCCCCAAATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATATTACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCggg                                                                                                                                                                                                               <  1:536170/235‑1 (MQ=255)
                          aCGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGGCTTTGGAAAAATTTTCGTGGGttttta                                                                                                                                                                                                       >  2:686488/1‑234 (MQ=255)
                          aCGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAAATGCGGCAACGTCAACGGAAATATTGTCGGGGGTTTTgc                                                                                                                                                                                                       >  2:97291/1‑235 (MQ=255)
                                  tccccATTTTCCGCGCAAGCTCAAGGCGGTATTCGTAAACATAAGTGATCCCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGtt                                                                                                                                                                                                <  1:409417/233‑1 (MQ=255)
                                      cATTTGCCGCGCCAGCTCAAGGCGTTATTCGTTAACATCAGTAACCCCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATg                                                                                                                                                                                           <  1:686488/236‑1 (MQ=255)
                                        ttttACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGCTCAAAAATTGCGGCTACGGCATCGGGAATATGTTCGGGGGTTTTGATAGGGTTGAGTGcc                                                                                                                                                                                         >  2:409417/1‑236 (MQ=255)
                                        ttttACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGcc                                                                                                                                                                                         >  1:719140/1‑236 (MQ=255)
                                        ttttACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGAATGCACGGCGTTACCGAAGGGGTCAAAAAATGCGGCTACGTACTCGGAAACAATGACGTGGATCTTGAATGCGTGTAATGcc                                                                                                                                                                                         >  2:478978/1‑236 (MQ=255)
                                        ttagacggaccAGCACAGGGTGTTTTTCGTAAACACCAGTGATCACCACATTGGGTGCACCACGTTGTTCCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGcc                                                                                                                                                                                         <  2:504495/227‑1 (MQ=255)
                                             cgcgCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGa                                                                                                                                                                                     >  1:578757/1‑235 (MQ=255)
                                             cgcgCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGa                                                                                                                                                                                     >  1:585787/1‑235 (MQ=255)
                                                 ccagtccAAGGCGGTTTTCGTTAACATCGGTGATCCCCCCATTGCGTCCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCAcc                                                                                                                                                                                <  1:63685/230‑1 (MQ=255)
                                                 ccAGCTCAAGGCGGTTTTCGTTAACATCAGTGATCCCCACATTGCGTGCACCAACGTTTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCAcc                                                                                                                                                                                <  1:749430/236‑1 (MQ=255)
                                                                         cATCGGGGATCACCAAATGGCGTCCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCgg                                                                                                                                                         <  1:416494/235‑1 (MQ=255)
                                                                              gTGATCACCACATTGCGTCCACCAGCGTTTTTCCCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGAtt                                                                                                                                                   <  1:634839/236‑1 (MQ=255)
                                                                                    accacATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAacac                                                                                                                                              >  2:758562/1‑235 (MQ=255)
                                                                                      cacaTTGCATGCACCAACGTTTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCaa                                                                                                                                           <  1:376112/236‑1 (MQ=255)
                                                                                         aTTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCa                                                                                                                                            >  2:308945/1‑232 (MQ=255)
                                                                                         aTTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTCTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCa                                                                                                                                            >  2:372451/1‑232 (MQ=255)
                                                                                                           ttccGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCgg                                                                                                                          <  1:90676/229‑1 (MQ=255)
                                                                                                                 aaCCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACAACTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATGTTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATggg                                                                                                                <  1:743257/235‑1 (MQ=255)
                                                                                                                    cgccgcTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTa                                                                                                              >  2:550921/1‑235 (MQ=255)
                                                                                                                          tGCAATAATCCCAAGCGGGCCAGCACCAGAAACCAGCACATCTTCCGCACACAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCCCCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTTCGGCACAAATGGGTACGaccac                                                                                                       <  2:147331/236‑1 (MQ=255)
                                                                                                                             cATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGaccac                                                                                                       <  2:440298/233‑1 (MQ=255)
                                                                                                                                     cAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTg                                                                                            <  1:689082/236‑1 (MQ=255)
                                                                                                                                       aTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCg                                                                                          >  2:646020/1‑236 (MQ=255)
                                                                                                                                       aTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCg                                                                                          <  1:478978/236‑1 (MQ=255)
                                                                                                                                       aTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCg                                                                                          >  2:764704/1‑236 (MQ=255)
                                                                                                                                            gCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGGCAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTTCGGCaa                                                                                      >  1:746517/1‑235 (MQ=255)
                                                                                                                                            gCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATGAACACCCAGGCCTATCGTGTTGCGGCACAAATGGGTACGACCAACACGAAAGTTgtacccag                                                                                     >  2:115967/1‑229 (MQ=255)
                                                                                                                                               tGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGAc                                                                                  >  1:401246/1‑236 (MQ=255)
                                                                                                                                                gCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAAt                                                                                     <  1:646020/232‑1 (MQ=255)
                                                                                                                                                gCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGAc                                                                                  <  1:372451/235‑1 (MQ=255)
                                                                                                                                                 caacataaaCCAGGCCATCTTCGCCCACCAGATCAAACGGCAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACca                                                                                <  2:746517/230‑1 (MQ=255)
                                                                                                                                                 cACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGAcc                                                                                 <  1:547017/235‑1 (MQ=255)
                                                                                                                                                   ccAGTAACTAGCACTGCTTCGCCCGCCAGTGCAAACGACAGCGCGGTAGTCACGGCTTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACcaca                                                                              <  2:131726/236‑1 (MQ=255)
                                                                                                                                                           cAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGAc                                                                                                 <  1:648707/209‑1 (MQ=255)
                                                                                                                                                           cAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGAc                                                                                                 >  2:648707/1‑209 (MQ=255)
                                                                                                                                                              cacaTCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACCAATGGGTACGACTACCACGACAGTTGCGGCAATGACCACAGGTGataatg                                                                    >  2:741540/1‑232 (MQ=255)
                                                                                                                                                                      cGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTTCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGcc                                                            >  1:180136/1‑235 (MQ=255)
                                                                                                                                                                                 aTCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGGGATATGGCCTTCGCTCGAAACGCGa                                                  >  2:544561/1‑235 (MQ=255)
                                                                                                                                                                                 aTCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGCGATATGGCCCTCGCCCGAAAcgcgc                                                  >  2:100033/1‑234 (MQ=255)
                                                                                                                                                                                     aaCGGCAGCGCGGTATTCACGGCGTTACCGAAGGGATCAAAACTTGCGGCTACGTCATCGGAAATATTGGCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGcc                                             <  2:719140/236‑1 (MQ=255)
                                                                                                                                                                                           aGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGGGATATGGCCTTCGCCAGAAACGCGATCGCCGATCt                                        >  1:598960/1‑235 (MQ=255)
                                                                                                                                                                                           aGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACACCAGGTGATATGGCCTTCGCCAGAAACCCGATAGCCGACCtt                                       >  2:524432/1‑236 (MQ=255)
                                                                                                                                                                                               cgGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAg                                   >  1:56733/1‑236 (MQ=255)
                                                                                                                                                                                                  tATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGGACGACCACCACGACAGTTCCGGCCATGACCACAGTGGCAATGGCCTTCGCCAGAAACCCGTACGCCGACCCTGAAAcc                                 >  2:35260/1‑235 (MQ=255)
                                                                                                                                                                                                    tGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAATCAGCTCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAAATACCACAGGTGATATGGCCTTCGCCAGAAACGCGAGTGCCACTCTTGCAGccg                                >  2:463165/1‑233 (MQ=255)
                                                                                                                                                                                                      cACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTc                             <  1:758562/235‑1 (MQ=255)
                                                                                                                                                                                                          gCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGGCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTc                        <  1:308945/236‑1 (MQ=255)
                                                                                                                                                                                                              tACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGTAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGAACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGa                    >  1:709626/1‑236 (MQ=255)
                                                                                                                                                                                                              tACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATAGCCGATCTTGAAGCCTTTCACTTCCTGa                    >  1:501728/1‑236 (MQ=255)
                                                                                                                                                                                                                   aaGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAt               <  1:544561/236‑1 (MQ=255)
                                                                                                                                                                                                                     gggATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCCATGACCCCAGGTAATATGGCCTTCGCCAGAAAAGCGATATCCGATCTTGAAGCCTTTCACTTTCTgtcactgac             >  2:584067/1‑228 (MQ=255)
                                                                                                                                                                                                                         tCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAtacct           >  1:701185/1‑234 (MQ=255)
                                                                                                                                                                                                                                ttGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCa  <  1:741540/236‑1 (MQ=255)
                                                                                                                                                                                                                                 tGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCa  >  1:450714/1‑235 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                  
AGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCA  >  NC_000913/3790687‑3791145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: