Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,802,005 T→G intergenic (‑6/+34) waaR ← / ← waaO lipopolysaccharide 1,2‑glucosyltransferase; UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase/UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑ alpha‑1,3‑D‑galactosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,802,0050TG96.3% 78.0 / ‑3.1 27intergenic (‑6/+34)waaR/waaOlipopolysaccharide 1,2‑glucosyltransferase; UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase/UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑ alpha‑1,3‑D‑galactosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (13/13);  total (14/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.97e-01

TGAAAAAAACCATCATTATAAACATCAGCAATAATATAAAAATCAAGATTAATATGTCGATTATTTAGGACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGC  >  NC_000913/3801782‑3802230
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 
tGAAAAAAACCATCATTATAAACATCAGCAATAATATAAAAATCAAGATTAATATGTCGATTATTTAGGACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCa                                                                                                                                                                                                                       >  2:367531/1‑236 (MQ=255)
tGAAAAAAACCATCATTATAAACATCAGCAATAATATAAAAATCAAGATTAATATGTCGATTATTTAGGACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCa                                                                                                                                                                                                                       <  1:367531/236‑1 (MQ=255)
                          aGCAATAATATAAAAATCAAGATTAATATGTCGATTATTTAGGACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCa                                                                                                                                                                                                                       >  1:19779/1‑210 (MQ=255)
                          aGCAATAATATAAAAATCAAGATTAATATGTCGATTATTTAGGACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCa                                                                                                                                                                                                                       <  2:19779/210‑1 (MQ=255)
                                 atatAAAAATCAAGATTAATATGTCGATTATTTAGGACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGtttt                                                                                                                                                                                                >  1:730/1‑226 (MQ=255)
                                                   atttGTCGATTATTTAGGACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCaataaaataa                                                                                                                                                                      <  2:122496/231‑1 (MQ=255)
                                                                            atgtgatgGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGa                                                                                                                                           >  1:173075/1‑236 (MQ=255)
                                                                                              cacCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCt                                                                                                                                                                                                                              >  2:636055/1‑135 (MQ=255)
                                                                                              cacCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCt                                                                                                                                                                                                                              <  1:636055/135‑1 (MQ=255)
                                                                                              cacCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGc                                                                                                                         >  1:187130/1‑236 (MQ=255)
                                                                                                   tCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTaaa                                                                                                                                                     >  1:272255/1‑203 (MQ=255)
                                                                                                   tCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTaaa                                                                                                                                                     <  2:272255/203‑1 (MQ=255)
                                                                                                     caGATAATTAGCATCGAATCCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGCATATGtt                                                                                                                  <  2:730/235‑1 (MQ=255)
                                                                                                     aaGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGCATATGtt                                                                                                                  <  2:187130/236‑1 (MQ=255)
                                                                                                           aTTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGCATATGTTTTgcgc                                                                                                            <  2:225080/236‑1 (MQ=255)
                                                                                                           aTTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGCATATGTTTTgcgc                                                                                                            >  1:225080/1‑236 (MQ=255)
                                                                                                                     acacCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGCATATGTTTTGCGCTATATc                                                                                                      >  1:615713/1‑232 (MQ=255)
                                                                                                                                 aCATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTAATTTCtc                                                                                        >  2:126880/1‑233 (MQ=255)
                                                                                                                                             tCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGCATATGTTTTGCGCt                                                                                                           <  1:39357/203‑1 (MQ=255)
                                                                                                                                             tCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGCATATGTTTTGCGCt                                                                                                           >  2:39357/1‑203 (MQ=255)
                                                                                                                                                              agctagcCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATa                                                                                                                                                              <  1:623667/135‑1 (MQ=255)
                                                                                                                                                              agctagcCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATa                                                                                                                                                              >  2:623667/1‑135 (MQ=255)
                                                                                                                                                                    ccGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCg                                                   >  1:80133/1‑236 (MQ=255)
                                                                                                                                                                                             tctatctCTATGGCAGGAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGc                          <  2:615713/236‑1 (MQ=255)
                                                                                                                                                                                                             gAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTa                                                                                                                                             >  1:535325/1‑105 (MQ=255)
                                                                                                                                                                                                             gAAATGAGTCCACAATGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTa                                                                                                                                             <  2:535325/105‑1 (MQ=255)
                                                                                                                                                                                                                         aaaTGCGACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGc  <  2:173075/231‑1 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 
TGAAAAAAACCATCATTATAAACATCAGCAATAATATAAAAATCAAGATTAATATGTCGATTATTTAGGACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTCCCAGTTTTAATGCTTTATCTTTTCAATAAAATAAAAAAGATAGTTGCTAAATCCTTTTAGATATCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGC  >  NC_000913/3801782‑3802230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: