Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 394,041 T→G pseudogene (1072/1449 nt) yaiT → pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913394,0410TG100.0% 229.1 / NA 65pseudogene (1072/1449 nt)yaiTpseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/37);  total (28/37)

TTCTGGTCAGGTGGATCAAGACAGCCAGACTGCCTACATCTACTCTTCTGCTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTTACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTACGTCTACGACGACTCTAACAACGATAACGATGTGAACGGCGATTCCATCGATAA  >  NC_000913/393809‑394267
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       
ttCTGGTCAGGTGGATCAAGACAGCCAGACTGCCTACATCTACTCTTCTGCTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACg                                                                                                                                                                                                                                      >  1:293005/1‑236 (MQ=255)
    ggTCAGGTGGATCAAGACAGCCAGACTGCCTACATCTACTCTTCTGCTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAg                                                                                                                                                                                                                                  <  2:293005/236‑1 (MQ=255)
    ggTCAGGTCGAGCAGGACATCCAGACTGCCTACAGCTACTCTTCTTCTCACTTCGCAAACAACGTCTTGGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGGCCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAg                                                                                                                                                                                                                                  <  2:665112/236‑1 (MQ=255)
                       gCCAGTCTGCCTACATCTGCTCTGCTGCTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGtt                                                                                                                                                                                                                   <  2:688370/232‑1 (MQ=255)
                        ccAGACTGCCTACATCTACTCTTCTGCTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTc                                                                                                                                                                                                                  >  1:502684/1‑232 (MQ=255)
                            aCTGCCTACATCTACTATTCTGCTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTc                                                                                                                                                                                                                  <  2:516232/228‑1 (MQ=255)
                                  tACATCTACTCTTCTGCTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTc                                                                                                                                                                                                                  <  2:741218/222‑1 (MQ=255)
                                  tACATCTACTCTTCTGCTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTc                                                                                                                                                                                                                  >  1:741218/1‑222 (MQ=255)
                                          ctctTCTGCTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGc                                                                                                                                                                                       >  2:648280/1‑236 (MQ=255)
                                               ctGCTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGt                                                                                                                                                                                                                        >  2:508528/1‑203 (MQ=255)
                                               ctGCTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGt                                                                                                                                                                                                                        <  1:508528/203‑1 (MQ=255)
                                                tGCTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCa                                                                                                                                                                                 >  2:417946/1‑236 (MQ=255)
                                                  cTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAAc                                                                                                                                                                               >  1:735373/1‑236 (MQ=255)
                                                         cgcgAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGa                                                                                                                                                                        >  1:606640/1‑236 (MQ=255)
                                                                     cTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTc                                                                                                                                                            <  1:80246/236‑1 (MQ=255)
                                                                     cTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTc                                                                                                                                                            <  2:606640/236‑1 (MQ=255)
                                                                      tttGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACg                                                                                                                                                               <  2:53891/232‑1 (MQ=255)
                                                                        tgttgATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGt                                                                                                                                                         <  2:306879/236‑1 (MQ=255)
                                                                              tgctAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACg                                                                                                                                                   <  2:325769/233‑1 (MQ=255)
                                                                               ggTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACg                                                                                                                                                   >  1:525703/1‑235 (MQ=255)
                                                                                    cTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCa                                                                                                                                             >  1:402453/1‑236 (MQ=255)
                                                                                     ttGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCAt                                                                                                                                            <  1:417946/236‑1 (MQ=255)
                                                                                      tGAGCTACTCTCACTTCACCAGCGGCCTGTCTGCAGCCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTGGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCAt                                                                                                                                            <  2:11008/235‑1 (MQ=255)
                                                                                          cTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGtt                                                                                                                                       <  2:502684/236‑1 (MQ=255)
                                                                                             ctctCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTc                                                                                                                                                            <  1:616242/212‑1 (MQ=255)
                                                                                             ctctCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTc                                                                                                                                                            >  2:616242/1‑212 (MQ=255)
                                                                                             ctctCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTa                                                                                                                                      >  2:418425/1‑234 (MQ=255)
                                                                                                 cACTTCAACCACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACt                                                                                                                                <  2:341427/236‑1 (MQ=255)
                                                                                                  aCTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTg                                                                                                                               >  1:129572/1‑236 (MQ=255)
                                                                                                        acaacGTCCGGGATGCAACCATGTGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACCAGCTGAGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGaa                                                                                                                                  <  2:716849/232‑1 (MQ=255)
                                                                                                            cgcccTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATg                                                                                                                     <  1:648280/233‑1 (MQ=255)
                                                                                                            cGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGt                                                                                                                                                                                                              <  2:528238/152‑1 (MQ=255)
                                                                                                            cGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGt                                                                                                                                                                                                              >  1:528238/1‑152 (MQ=255)
                                                                                                             gACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGc                                                                                                                    <  2:525703/236‑1 (MQ=255)
                                                                                                                cTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATg                                                                                                                     <  2:764350/232‑1 (MQ=255)
                                                                                                                             tGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCAcc                                                                                                    >  2:292726/1‑236 (MQ=255)
                                                                                                                             tGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCAcc                                                                                                    >  2:252396/1‑236 (MQ=255)
                                                                                                                             tGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCAcc                                                                                                    >  1:623744/1‑236 (MQ=255)
                                                                                                                               aGCCACGGTTCTGACGTTAACGGTAACACCAGATACGACGATTGGGGCTTCGGTTTGAAAGCCGGTTACGTCTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGACTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTa                                                                                                  <  2:705189/236‑1 (MQ=255)
                                                                                                                                    cGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTg                                                                                                                                                                  <  2:337163/167‑1 (MQ=255)
                                                                                                                                    cGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTg                                                                                                                                                                  >  1:337163/1‑167 (MQ=255)
                                                                                                                                    cGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCg                                                                                             >  2:569981/1‑236 (MQ=255)
                                                                                                                                           tACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCa                                                                                      <  1:140150/236‑1 (MQ=255)
                                                                                                                                                 gACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATg                                                                                                                     <  1:114296/199‑1 (MQ=255)
                                                                                                                                                 gACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATg                                                                                                                     >  2:114296/1‑199 (MQ=255)
                                                                                                                                                 gACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTAACCTACAGCGAAGATCAGGctc                                                                                 >  1:584379/1‑235 (MQ=255)
                                                                                                                                                              aCTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGTCTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACt                                                                                                                                <  2:335329/175‑1 (MQ=255)
                                                                                                                                                              aCTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACt                                                                                                                                >  1:335329/1‑175 (MQ=255)
                                                                                                                                                                  cGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAAc                                                               <  1:418425/236‑1 (MQ=255)
                                                                                                                                                                        ttGGGGCTTCGGTGTGAACGCCGGTTGCGACTTCAAACTGGGTGATGCTGGTTACGTGACGCCTGACGGCAGCTGTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCtt                                                         <  2:549442/236‑1 (MQ=255)
                                                                                                                                                                         tGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTa                                                        <  2:129572/236‑1 (MQ=255)
                                                                                                                                                                         tGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTa                                                        <  2:402453/236‑1 (MQ=255)
                                                                                                                                                                                   gttgtaaaGCCGGTTACGACTTAAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTACGTCTacgac                                              <  2:735373/231‑1 (MQ=255)
                                                                                                                                                                                        aaaGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCCCTGACTCCGTACTTCAAACTGGCTTACGTCTACGACGActc                                         >  2:640548/1‑236 (MQ=255)
                                                                                                                                                                                                               gggTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTACGTCTACGACGACTCTAACAACGATAACGATGTGAACg                  >  2:21395/1‑236 (MQ=255)
                                                                                                                                                                                                                 gTGATGCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTACGTCTACGACGACTCTAACAACGATAACGATGTGAACGGc                >  1:71744/1‑236 (MQ=255)
                                                                                                                                                                                                                      gCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTACGTCTACGACGACTCTAACAACGATAACGATGTGAACGGCGAtt            <  2:623744/235‑1 (MQ=255)
                                                                                                                                                                                                                      gCTGGTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTACGTCTACGACGACTCTAACAACGATAACGATGTGAACGGCGAtt            <  1:21395/235‑1 (MQ=255)
                                                                                                                                                                                                                          gTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTACGTCTacgac                                              >  1:545500/1‑197 (MQ=255)
                                                                                                                                                                                                                          gTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTACGTCTacgac                                              <  2:545500/197‑1 (MQ=255)
                                                                                                                                                                                                                          gTTACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTACGTCTACGACGACTCTAACAACGATAACGATGTGAACGGCGATTCCATc       >  2:92275/1‑236 (MQ=255)
                                                                                                                                                                                                                            tACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTg                                                                                                                               >  2:559906/1‑114 (MQ=255)
                                                                                                                                                                                                                            tACGTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTg                                                                                                                               <  1:559906/114‑1 (MQ=255)
                                                                                                                                                                                                                               gTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTACGTCTACGACGACTCTAACAACGATAACGATGTGAACGGCGATTCCATCGATaa  <  1:252396/236‑1 (MQ=255)
                                                                                                                                                                                                                               gTGACTCCTGACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTACGTCTACGACGACTCTAACAACGATAACGATGTGAACGGCGATTCCATCGATaa  <  1:292726/236‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       
TTCTGGTCAGGTGGATCAAGACAGCCAGACTGCCTACATCTACTCTTCTGCTCACTTCGCGAACAACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCAAACTGGGTGATGCTGGTTACGTGACTCCTTACGGCAGCGTTTCTGGTCTGTTCCAGTCTGGTGATGACTACC‑‑‑‑‑AGCTGAGCAACGACATGAAAGTTGACGGTCAGTCTTACGACAGCATGCGTTATGAACTGGGTGTAGATGCAGGTTATACCTTCACCTACAGCGAAGATCAGGCTCTGACTCCGTACTTCAAACTGGCTTACGTCTACGACGACTCTAACAACGATAACGATGTGAACGGCGATTCCATCGATAA  >  NC_000913/393809‑394267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: