Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,012,707	A→C	F37C (TTT→TGT)	metR ←	methionine biosynthesis regulon transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,012,707	0	A	C	100.0%	105.4 / NA	32	F37C (TTT→TGT)	metR	methionine biosynthesis regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/26);  total (6/26)

GTTCTTATGGAAATTTTCTAACGCGGGTGTCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTAAACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATCCTTCGTCTTTTA  >  NC_000913/4012475‑4012921                                                                                                                                                                                                                                         |                                                                                                                                                                                                                       gTTCTTATGGAAATTTTCTAACGCGGGTGTCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTacac                                                                                                                                                                                                                      <  2:470165/235‑1 (MQ=255)                  cTAACGCGGGTGTCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGAt                                                                                                                                                                                                    <  1:7670/236‑1 (MQ=255)                                      tGAATTCAGCTATGGCACTAAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAAc                                                                                                                                                                                     <  1:195458/231‑1 (MQ=255)                                      tGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGGTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGt                                                                                                                                                                                   <  1:567432/233‑1 (MQ=255)                                                  tGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCCGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCCCTCATAGCCGGAAGCCCAGGCGTTGTTCCAGCTCGCTTCACTGGTCGGACCGGGCGGATTGCGTCTGGTGCAAGGTCGCCGAAGCGGCTgg                                                                                                                                                                    >  2:152371/1‑235 (MQ=255)                                                   ggCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTGGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCt                                                                                                                                                                      <  1:152371/233‑1 (MQ=255)                                                            tGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCg                                                                                                                                                          <  1:766421/236‑1 (MQ=255)                                                                     gcAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGc                                                                                                                                                 <  1:170616/236‑1 (MQ=255)                                                                        gACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCaa                                                                                                                                               <  2:557791/235‑1 (MQ=255)                                                                        gACGCGTCTGCTGCGGTTAATTGCAGGCTTGCAGGTCCGGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCaa                                                                                                                                               <  2:195504/235‑1 (MQ=255)                                                                                          catgtcAGGCTTGCAGGGCCTGGTTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAg                                                                                                                             <  2:195836/230‑1 (MQ=255)                                                                                          cATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAg                                                                                                                             <  1:337164/235‑1 (MQ=255)                                                                                                      gCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACt                                                                                                                   <  1:681710/233‑1 (MQ=255)                                                                                                             cTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTacac                                                                                                                                                                                                                      <  2:175417/127‑1 (MQ=255)                                                                                                                    attgggagcggTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTcc                                                                                                   <  2:287285/225‑1 (MQ=255)                                                                                                                                          ccAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTg                                                                            <  2:686284/236‑1 (MQ=255)                                                                                                                                                  aaCAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCtt                                                                       <  1:733673/233‑1 (MQ=255)                                                                                                                                                  aaCAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGa                                                                     <  1:194746/235‑1 (MQ=255)                                                                                                                                                  aaCAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAg                                                                    <  1:385475/236‑1 (MQ=255)                                                                                                                                                  aaCAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAg                                                                    <  2:581649/236‑1 (MQ=255)                                                                                                                                                  aaCAGGATTTCCCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCtt                                                                       <  1:628260/233‑1 (MQ=255)                                                                                                                                                    ctggATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGa                                                                  <  2:343686/234‑1 (MQ=255)                                                                                                                                                     aGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGaa                                                                 <  1:368684/236‑1 (MQ=255)                                                                                                                                                           tctcCCTGCGGTGTAAAGGGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGa                                                                  <  1:457290/229‑1 (MQ=255)                                                                                                                                                                  gCGGTGAAAGGCGTAGCGGCTGGCTCTTACGCACAAATGGCCGGAAGCCAAGGCGTTTTTCCAGATCGCTACACTGGTGAGACGGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTCCTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCg                                                    <  2:509068/236‑1 (MQ=255)                                                                                                                                                                              gTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGAt                                        <  1:551095/236‑1 (MQ=255)                                                                                                                                                                                                           cGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATcc             >  2:470406/1‑234 (MQ=255)                                                                                                                                                                                                              aaGCCAGGGCTTTGTTCCAGATCGCTACCCTGGTGGGCCGGGGCGGATTGCGTCTGATGCAACGTCGCCGCGGCGGCTGCGAGCGAGCCGCAGTTCCGAAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATCCTTc          <  1:319763/234‑1 (MQ=255)                                                                                                                                                                                                              aaGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATCCt            >  1:505249/1‑232 (MQ=255)                                                                                                                                                                                                              aaGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATCCTTCg         <  1:470406/235‑1 (MQ=255)                                                                                                                                                                                                                    aGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCCGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATCCTTCGACTTTTa  >  1:763186/1‑236 (MQ=255)                                                                                                                                                                                                                                      cTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGa                                                                  >  1:476091/1‑154 (MQ=255)                                                                                                                                                                                                                                      cTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGa                                                                  <  2:476091/154‑1 (MQ=255)                                                                                                                                                                                                                                      cTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTa                                             >  1:646669/1‑175 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                       GTTCTTATGGAAATTTTCTAACGCGGGTGTCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTAAACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATCCTTCGTCTTTTA  >  NC_000913/4012475‑4012921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑