Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,046,716	A→C	intergenic (‑114/‑250)	yihG ← / → polA	inner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,046,716	0	A	C	100.0%	188.0 / NA	51	intergenic (‑114/‑250)	yihG/polA	inner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/25);  total (26/25)

GATGATCGGGACGGAACAAAAAATAGTGACCAAAATGGTCAATACGATACTCAGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTG  >  NC_000913/4046489‑4046946                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       gatgatCGGGACGGAACAAAAAATAGTGACCAAAATGGTCAATACGATACTCAGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCa                                                                                                                                                                                                                                <  2:345270/236‑1 (MQ=255) gatgatCGGGACGGAACAAAAAATAGTGACCAAAATGGTCAATACGATACTCAGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCa                                                                                                                                                                                                                                >  1:478007/1‑236 (MQ=255)      tCGGGACGGAACAAAAAATAGTGACCAAAATGGTCAATACGATACTCAGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAattat                                                                                                                                                                                                                           <  2:101167/236‑1 (MQ=255)                  aaaaaaTAGTGACCAAAATGGTCAATACGATACTCAGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGggatggat                                                                                                                                                                                                                <  1:22821/235‑1 (MQ=255)                  aaaaaaTAGTGACCAAAATGGTCAATACGATACTCAGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGggatggat                                                                                                                                                                                                                <  2:478007/235‑1 (MQ=255)                           tGACCAAAATGGTCAATACGATACTCAGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCAACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGTTGGATTTATTTTTa                                                                                                                                                                                                       >  2:481638/1‑235 (MQ=255)                               cAAAATGGTCAATACGATACTCAGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTaa                                                                                                                                                                                                      <  1:13738/232‑1 (MQ=255)                                              gATACTCAGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGAc                                                                                                                                                                                   <  1:84720/236‑1 (MQ=255)                                                tACTCAGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACaa                                                                                                                                                                                 >  2:298185/1‑236 (MQ=255)                                                   tCAGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAAca                                                                                                                                                                              <  1:298185/236‑1 (MQ=255)                                                   tCAGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAAca                                                                                                                                                                              <  1:754314/236‑1 (MQ=255)                                                     aGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTT‑GCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAAcat                                                                                                                                                                             >  1:186462/1‑234 (MQ=255)                                                                 aTCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTaaaaa                                                                                                                                                                >  2:589147/1‑236 (MQ=255)                                                                 aTCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTaaaaa                                                                                                                                                                >  1:522643/1‑236 (MQ=255)                                                                            tATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAAAGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGCAAAATACTTGATATCTATCCACATTAGACAAAATCCCCCTATATCAATGATTAGGGATGGATTTATTTTTAACAGCATGAAAAAAAAAGACAAACATCATGATGTATAAATCATGataata                                                                                                                                                     >  2:660197/1‑236 (MQ=255)                                                                                                    cAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAAttt                                                                                                                             <  2:162241/236‑1 (MQ=255)                                                                                                            tAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATCACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGc                                                                                                                         <  2:359602/232‑1 (MQ=255)                                                                                                            tAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGc                                                                                                                         >  1:359602/1‑232 (MQ=255)                                                                                                                         tGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTaaaa                                                                                                                                                                 <  1:605219/179‑1 (MQ=255)                                                                                                                         tGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTaaaa                                                                                                                                                                 >  2:605219/1‑179 (MQ=255)                                                                                                                         tGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCg                                                                                                                                          >  2:511644/1‑202 (MQ=255)                                                                                                                         tGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCg                                                                                                                                          <  1:511644/202‑1 (MQ=255)                                                                                                                         tGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGc                                                                                                                         >  1:570876/1‑219 (MQ=255)                                                                                                                         tGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGc                                                                                                                         <  2:570876/219‑1 (MQ=255)                                                                                                                         tGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATg                                                                                                        >  1:37631/1‑236 (MQ=255)                                                                                                                         tGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATg                                                                                                        >  2:238944/1‑236 (MQ=255)                                                                                                                          gcgcTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGa                                                                                                       >  1:304701/1‑236 (MQ=255)                                                                                                                                       gcAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgagcg                                                                                          <  1:589147/236‑1 (MQ=255)                                                                                                                                       gcAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgag                                                                                            <  2:522643/234‑1 (MQ=255)                                                                                                                                       gcAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgag                                                                                            <  1:238944/234‑1 (MQ=255)                                                                                                                                                       aGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGc                                                                                                                                           <  1:150190/171‑1 (MQ=255)                                                                                                                                                       aGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGc                                                                                                                                           >  2:150190/1‑171 (MQ=255)                                                                                                                                                                                 gatCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGa                                                  >  2:193094/1‑234 (MQ=255)                                                                                                                                                                                 gatCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGa                                                  >  1:532969/1‑234 (MQ=255)                                                                                                                                                                                              aaaaaTAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCaa                                                                                                                                                                                                                               <  2:194480/48‑1 (MQ=255)                                                                                                                                                                                              aaaaaTAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCaa                                                                                                                                                                                                                               >  1:194480/1‑48 (MQ=255)                                                                                                                                                                                              aaaaaTAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGaa                                                           <  2:552917/212‑1 (MQ=255)                                                                                                                                                                                              aaaaaTAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGaa                                                           >  1:552917/1‑212 (MQ=255)                                                                                                                                                                                              aaaaaTAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACt                                            >  1:565992/1‑227 (MQ=255)                                                                                                                                                                                              aaaaaTAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACt                                            <  2:565992/227‑1 (MQ=255)                                                                                                                                                                                              aaaaaTAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGtttt                                    >  1:430599/1‑235 (MQ=255)                                                                                                                                                                                                           atctatcCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGAc                                                                                                                                                                                   >  2:604241/1‑79 (MQ=255)                                                                                                                                                                                                           atctatcCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATAAAAAACAAAGAc                                                                                                                                                                                   <  1:604241/79‑1 (MQ=255)                                                                                                                                                                                                                tcCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGc                                                                                                                                           >  1:319877/1‑114 (MQ=255)                                                                                                                                                                                                                tcCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGc                                                                                                                                           <  2:319877/114‑1 (MQ=255)                                                                                                                                                                                                                tcCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCAt                                 >  2:59976/1‑220 (MQ=255)                                                                                                                                                                                                                tcCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCAt                                 <  1:59976/220‑1 (MQ=255)                                                                                                                                                                                                                tcCACATTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATcc                 >  1:440599/1‑236 (MQ=255)                                                                                                                                                                                                                     aTTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAAcatcat                                                                                                                                                                          <  2:704855/78‑1 (MQ=255)                                                                                                                                                                                                                     aTTAGAAAAAATCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAAcatcat                                                                                                                                                                          >  1:704855/1‑78 (MQ=255)                                                                                                                                                                                                                               aTCCCCTTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTg  <  1:193094/236‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       GATGATCGGGACGGAACAAAAAATAGTGACCAAAATGGTCAATACGATACTCAGCAAAAGGGTTATCGCAGCGAGTATTCTCGTCATAATGAATTTATTCAAAAGGTTAGCCATAGACAGTGCGCTAAAGAAGCGCAAGGCGCAGATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTG  >  NC_000913/4046489‑4046946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑