Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	400,871	T→G	E18A (GAA→GCA)	ddlA ←	D‑alanine‑D‑alanine ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	400,871	0	T	G	100.0%	248.5 / NA	70	E18A (GAA→GCA)	ddlA	D‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/39);  total (31/39)

CGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTTCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTACTGGTGAAGAAAGTGCGTTATCTCAAAGATGTGCGCAAGATCACAAAAATGATGAACGGG  >  NC_000913/400654‑401096                                                                                                                                                                                                                          |                                                                                                                                                                                                                                  cgcgTCGATAAGCTGATGCTCATGTTTCCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGAGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCAATTGCAGAGACACTGCATGTTCCGCTGATTTac                                                                                                                                                                                                                 <  1:91283/236‑1 (MQ=255)          aaGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCaaaaa                                                                                                                                                                                                        >  2:9294/1‑236 (MQ=255)                aTGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGAtt                                                                                                                                                                                                   >  2:560897/1‑235 (MQ=255)                aTGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGAtt                                                                                                                                                                                                   >  2:251628/1‑235 (MQ=255)                aTGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACAACCAAAAACGAtt                                                                                                                                                                                                   >  2:453624/1‑235 (MQ=255)                                ggCACCTGCGCAATGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCCCCAAAAACGATTCCTACCCCCCGTTTTTc                                                                                                                                                                                  >  2:212994/1‑236 (MQ=255)                                ggCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAcc                                                                                                                                                                                             >  2:86578/1‑225 (MQ=255)                                ggCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAcc                                                                                                                                                                                             <  1:86578/225‑1 (MQ=255)                                  cACCTTGGCAAGGCATGGCGCAGAAGGGCGCAACGCAATATGGGCAGGATCCGCTTGATTTAACAGAAAATTGCCGGCATCGCTGACGTGCCATTTCCCCTTTTTAACAAAGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCCACAATGGTTTTTGCCGATTGCAGAGACCCTGCATGTTCCCCTGATTTACCCCCAAAAACCATTTCTACCCGCAGGTTTTCCa                                                                                                                                                                                <  2:141426/236‑1 (MQ=255)                                  cACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaccacc                                                                                                                                                                                                             <  2:369809/207‑1 (MQ=255)                                  cACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaccacc                                                                                                                                                                                                             >  1:369809/1‑207 (MQ=255)                                     cTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCt                                                                                                                                                                             >  2:395185/1‑236 (MQ=255)                                       gcgcAAGGCTGGTACCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTa                                                                                                                                                                           <  2:334966/236‑1 (MQ=255)                                        cgcAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTa                                                                                                                                                                           >  1:146859/1‑235 (MQ=255)                                         gcAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaaa                                                                                                                                                                         <  1:343334/236‑1 (MQ=255)                                              gCTGGTCGCCGAAGGGCGCAACGCAATAGGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCt                                                                                                                                                                    <  1:254048/236‑1 (MQ=255)                                                  gttgCCTAAGGGCCCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAGCGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATc                                                                                                                                                                 <  2:645426/232‑1 (MQ=255)                                                  gTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCaaaaa                                                                                                                                                                                                        >  2:409241/1‑196 (MQ=255)                                                  gTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCaaaaa                                                                                                                                                                                                        <  1:409241/196‑1 (MQ=255)                                                  gTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAAcc                                                                                                                                                                     >  1:112096/1‑231 (MQ=255)                                                  gTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATc                                                                                                                                                                 <  2:315873/235‑1 (MQ=255)                                                  gTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATc                                                                                                                                                                 <  1:395185/235‑1 (MQ=255)                                                  gTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATc                                                                                                                                                                 <  1:213460/235‑1 (MQ=255)                                                  gTCGACGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATc                                                                                                                                                                 <  2:307136/235‑1 (MQ=255)                                                   tCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATc                                                                                                                                                                 <  1:595561/234‑1 (MQ=255)                                                      ccGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGt                                                                                                                                                             <  1:250351/235‑1 (MQ=255)                                                            ggCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAacac                                                                                                                                                      >  2:16550/1‑236 (MQ=255)                                                             gcgcAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAAcaca                                                                                                                                                     <  1:560897/236‑1 (MQ=255)                                                             gcgcAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAAcaca                                                                                                                                                     <  2:146859/236‑1 (MQ=255)                                                             gcgcAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAAcaca                                                                                                                                                     <  2:140008/236‑1 (MQ=255)                                                              cgcaacgcaaTATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACCCTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTTAAAACCTATCCCGCCTaatccaa                                                                                                                                                    >  2:68806/1‑231 (MQ=255)                                                                    gcaaTATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCa                                                                                                                                              <  1:16550/236‑1 (MQ=255)                                                                    gcaaTATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCa                                                                                                                                              <  1:212994/236‑1 (MQ=255)                                                                    gcaaTATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCa                                                                                                                                              <  1:9294/236‑1 (MQ=255)                                                                              cAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACCTTACCa                                                                                                                                    >  2:182045/1‑236 (MQ=255)                                                                                ggATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGAATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCatg                                                                                                                                  >  1:9953/1‑234 (MQ=255)                                                                                     gTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACaa                                                                                                                              <  2:112096/235‑1 (MQ=255)                                                                                      tCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGAtt                                                                                                                                                                                                   >  2:518080/1‑165 (MQ=255)                                                                                      tCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGAtt                                                                                                                                                                                                   <  1:518080/165‑1 (MQ=255)                                                                                           aTTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACgggg                                                                                                                       >  1:414047/1‑236 (MQ=255)                                                                                                    aTAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTACAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCgg                                                                                                              >  1:368640/1‑236 (MQ=255)                                                                                                    aTAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCgg                                                                                                              >  1:579455/1‑236 (MQ=255)                                                                                                      aaTTGCTGGCATCGCTTACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGcc                                                                                                            <  1:307623/236‑1 (MQ=255)                                                                                                        ttGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCGAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATCACCACGACAAAACGGGGGATTCGCGGcc                                                                                                            <  1:743593/234‑1 (MQ=255)                                                                                                           cTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTg                                                                                                       <  1:453624/236‑1 (MQ=255)                                                                                                              gCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAATTGCATACATTACCACGACAAAACGGGGGATTCGCGTACTTCTGa                                                                                                      >  2:743593/1‑234 (MQ=255)                                                                                                                aTCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGTGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGTGGGATTCCCGGCCTTCTgc                                                                                                      >  2:709647/1‑231 (MQ=255)                                                                                                                aTCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGa                                                                                                  >  2:613755/1‑236 (MQ=255)                                                                                                                aTCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCACGGCCTTCTGa                                                                                                      >  1:666518/1‑232 (MQ=255)                                                                                                                        cGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTAcc                                                                                                                                     <  2:119868/193‑1 (MQ=255)                                                                                                                        cGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTAcc                                                                                                                                     >  1:119868/1‑193 (MQ=255)                                                                                                                                     ttGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACTCTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGAc                                                                             <  2:579455/236‑1 (MQ=255)                                                                                                                                           aTCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCg                                                                       <  1:251628/236‑1 (MQ=255)                                                                                                                                             cAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTg                                                                     <  1:421118/236‑1 (MQ=255)                                                                                                                                               aTGGCCATCAACACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCa                                                                   <  2:655248/236‑1 (MQ=255)                                                                                                                                                tGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTctg                                                                                  <  1:11776/220‑1 (MQ=255)                                                                                                                                                tGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTctg                                                                                  >  2:11776/1‑220 (MQ=255)                                                                                                                                                    cagcagCAAAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTa                                                              <  1:202792/236‑1 (MQ=255)                                                                                                                                                      gcagcaCAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTACt                                                            <  1:503801/236‑1 (MQ=255)                                                                                                                                                            caACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTa                                                                                                                                                                           <  2:451307/119‑1 (MQ=255)                                                                                                                                                            caACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTa                                                                                                                                                                           >  1:451307/1‑119 (MQ=255)                                                                                                                                                               cGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTACTGGTgaagaa                                                   >  2:640883/1‑236 (MQ=255)                                                                                                                                                                      gCGACTTTTATCAATGTCCTCGTCAATTTTTTTTGCCGATTGCAGAGTCACTGCATGTTCCGCTGATTTACCACCAAAACCGCTTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTACTGGTGAAGAAAGTGCGt                                            <  2:9953/236‑1 (MQ=255)                                                                                                                                                                      gCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTACTGGTGAAGAAAGTGCGt                                            <  1:640883/236‑1 (MQ=255)                                                                                                                                                                                        aTCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTg                                                                     <  2:425006/193‑1 (MQ=255)                                                                                                                                                                                        aTCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTg                                                                     >  1:425006/1‑193 (MQ=255)                                                                                                                                                                                                tGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAATCGTGTAACGTACTGGTGAAGAACGTGCGTTATCTCAACGATGCTCGCACGATcac                  >  2:1119/1‑236 (MQ=255)                                                                                                                                                                                                tGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTACTGGTGAAGAAAGTGCGTTATCTCAAAGATGTGCGCAAGATcac                  >  1:153198/1‑236 (MQ=255)                                                                                                                                                                                                            aTTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTACTGGTGAAGAAAGTGCGTTATCTCAAAGATGTGCGCAAGATCACAAAAATGATGaa      >  1:763491/1‑236 (MQ=255)                                                                                                                                                                                                                cAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTACTGGTGAAGAAAGTGCGTTATCTCAAAGATGTGCGCAAGATCACAAAAATGATGAACggg  <  1:613755/236‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                  CGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTTCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTACTGGTGAAGAAAGTGCGTTATCTCAAAGATGTGCGCAAGATCACAAAAATGATGAACGGG  >  NC_000913/400654‑401096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑