Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	402,002	T→G	Y86D (TAT→GAT)	phoA →	bacterial alkaline phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	402,002	0	T	G	100.0%	176.8 / NA	54	Y86D (TAT→GAT)	phoA	bacterial alkaline phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/26);  total (28/26)

ACTCTTACCGTTACTGTTTACCCCTGTGACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGATATTCACGAAAAAGATCACCCAACGATTCTGGAAATGGCAAAAGCCG  >  NC_000913/401773‑402227                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                  aCTCTTACCGTTACTGTTTACCCCTGTGACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGATTCGGAAATTACTGCCGCACGTCATGCTGCCg                                                                                                                                                                                                                             >  2:435473/1‑236 (MQ=255)         cGTTACTGTTTACCCCTGTGACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTgcg                                                                                                                                                                                                                     >  1:453156/1‑236 (MQ=255)             aCTGTTTACCCCTGTGACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTgcgggcg                                                                                                                                                                                                                 >  1:466438/1‑236 (MQ=255)                         tgtgACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAACCCGGGCTGCTAAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAg                                                                                                                                                                                                     <  2:202483/236‑1 (MQ=255)                         tgtgACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAg                                                                                                                                                                                                     >  2:271482/1‑236 (MQ=255)                         tgtgACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAg                                                                                                                                                                                                     >  2:611392/1‑236 (MQ=255)                         tgtgACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCGTTTTTAAAg                                                                                                                                                                                                     >  1:46285/1‑236 (MQ=255)                           tgACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGTCGGCTTTTTTAAAGGt                                                                                                                                                                                                   >  1:279565/1‑236 (MQ=255)                                aaaGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAg                                                                                                                                                                                               >  1:576804/1‑235 (MQ=255)                                  aGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATg                                                                                                                                                                                            <  2:576804/236‑1 (MQ=255)                                     ccGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGa                                                                                                                                                                                              <  1:611392/231‑1 (MQ=255)                                       ggACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCctta                                                                                                                                                                                       <  1:268917/236‑1 (MQ=255)                                            ccAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCcttaccgc                                                                                                                                                                                   >  1:176527/1‑235 (MQ=255)                                            ccAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCcttaccgc                                                                                                                                                                                   >  1:489266/1‑235 (MQ=255)                                              aGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCcttaccgctta                                                                                                                                                                                <  2:453156/236‑1 (MQ=255)                                              aGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCcttaccgctta                                                                                                                                                                                <  1:271482/236‑1 (MQ=255)                                                    gCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCgg                                                                                                                                                                            <  1:639334/234‑1 (MQ=255)                                                           cTGGAAAACCGGGCTGCTCAGGTCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATacac                                                                                                                                                                   <  2:176527/236‑1 (MQ=255)                                                           cTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATacac                                                                                                                                                                   <  2:489266/236‑1 (MQ=255)                                                                      ggCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATgcgc                                                                                                                                                        >  1:460223/1‑236 (MQ=255)                                                                                     tatTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGAATAGATGCCTTACCGCTTACCGGGCACTACACTCACTATGCGCTGAATAAATAAACCg                                                                                                                                         >  2:295219/1‑236 (MQ=255)                                                                                          cTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCaaa                                                                                                                                    <  2:279565/236‑1 (MQ=255)                                                                                             cACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATacac                                                                                                                                                                   <  1:182990/202‑1 (MQ=255)                                                                                             cACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATacac                                                                                                                                                                   >  2:182990/1‑202 (MQ=255)                                                                                               cccGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCgg                                                                                                                                >  1:617028/1‑235 (MQ=255)                                                                                               cccGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCgg                                                                                                                                >  1:134766/1‑235 (MQ=255)                                                                                                  ggcggTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAACAAAACCGGCAAACCGGACt                                                                                                                             >  1:650289/1‑235 (MQ=255)                                                                                                    cggTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGTGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTAc                                                                                                                           >  1:2618/1‑235 (MQ=255)                                                                                                                 ttAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCgg                                                                                                              <  2:460223/235‑1 (MQ=255)                                                                                                                        gTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCa                                                                                                      >  2:313427/1‑236 (MQ=255)                                                                                                                            acgtaCTGCCGCTGTGGTAGATTCTCTGAGCGATAAACCTGCAAAAGATATTATTTTGCTGATTGGAGATGGGATGGGGGACGAGGAATTTACTGCCGCACGTAATGATGCCGAAGGTGGGGGCGGTTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATCCCCTCACTATGCGCTGAATAAAAAAACCGGCAAACCGTACTACGTCCCCGACTCGGCTGCATCAGCa                                                                                                   <  2:264347/231‑1 (MQ=255)                                                                                                                                                  tttctTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACGGCCGCACGTAATGATGCGGAAGTTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCa                                                                             <  1:445055/233‑1 (MQ=255)                                                                                                                                                  tctcTTGACGATAAACATGCAAAAAATATAATTTTGCTGATTGGCCATGGGAGGGGGACCTCGGAAATTACGGCCGCACTTATGGATCCCGAAGGTGCGGGCGACTTTTTTAAGGGTATAGATGCCTTACCGCTTACCGGGCAATCCCCTCCCTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCa                                                                             <  2:650289/235‑1 (MQ=255)                                                                                                                                                  tctcGTAGCAATAAACCGGTAAAAAATATTATTATGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACGGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTCCCGCTTACCGGCCAATACACTCCCTATGCGCTGAATAAAAAAACCGGCAAACCGGCCTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCa                                                                             <  2:549801/235‑1 (MQ=255)                                                                                                                                                  tctataggcaaTAAACCGGAAAAAATTATTATTTGGATGATGGGCGATGGGATGGGGGACTCGGAATTTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCa                                                                             <  2:134766/225‑1 (MQ=255)                                                                                                                                                    tctTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCaaa                                                                           <  1:313427/235‑1 (MQ=255)                                                                                                                                                      ttAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAAcc                                                                        >  1:351656/1‑236 (MQ=255)                                                                                                                                                                     cAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTg                                                         >  1:668977/1‑236 (MQ=255)                                                                                                                                                                                      cTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCTTCGaactttccga                                        >  2:99219/1‑227 (MQ=255)                                                                                                                                                                                       tgttgggCGAGGGGATGGAGCACTGGGAAATTACTGCCGCGTGTAATGATGCCGAAGGTGTGGGCGGCTTTTTAAAAGGTATAGATCCCTTACCGCTTACCGAGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGATATTCACGaa                                       <  2:2618/231‑1 (MQ=255)                                                                                                                                                                                                   ggatgggGGACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGATATTCACGAAAAAGATCAACCa                           >  1:617869/1‑236 (MQ=255)                                                                                                                                                                                                        ggggACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTaa                                                                                                                                                                                                       >  2:300615/1‑59 (MQ=255)                                                                                                                                                                                                        ggggACTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTaa                                                                                                                                                                                                       <  1:300615/59‑1 (MQ=255)                                                                                                                                                                                                             cTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGc                                                                                              <  1:702829/159‑1 (MQ=255)                                                                                                                                                                                                             cTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGc                                                                                              >  2:702829/1‑159 (MQ=255)                                                                                                                                                                                                             cTCGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTCGGCGTCGATATTCACGACAAAGATCACCCAACGATTCTgg                 >  1:26993/1‑236 (MQ=255)                                                                                                                                                                                                             cTCGGAAATTACTCCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGGCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGCTGCGCCGGCCGTCGAAATTCACGAAAAAGAATACCATACGAtgttgc                 >  2:259249/1‑231 (MQ=255)                                                                                                                                                                                                               cGGAAATTACTGCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGAACTTCACGAAAAAGATAACCCAACGATTCTGGaa               >  2:448985/1‑236 (MQ=255)                                                                                                                                                                                                                      ttctaccggtggTAATGTTGCCGAAGGTGCGGGCGTCTTTTTAAAAGGTATAGATCCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAAAAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGATATTCACGAAAAAGATCACCCAACGATTCTGGAAATGGCa         <  2:466438/224‑1 (MQ=255)                                                                                                                                                                                                                       acggacGCACGTAAGGATGCCGAAGGTGCGGTCGTCTTTTTGAAGGGTATAGATGCCTTACCGCTGACCGGGCAATCCACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGATATTCACGAAAAAGATCACCCAACGATTCTGGAAATGGCaaa       <  2:151046/231‑1 (MQ=255)                                                                                                                                                                                                                          gCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCa                                                                                                                                      <  2:57064/106‑1 (MQ=255)                                                                                                                                                                                                                          gCCGCACGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCa                                                                                                                                      >  1:57064/1‑106 (MQ=255)                                                                                                                                                                                                                            cgaaCGTAATGATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGAATAGAGGCCTTACCGCTTACCGGCCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGATATTCACGAAAAAGATCACCCAACGATTCTGGAAATGGCAAAAGCCg  <  1:295219/233‑1 (MQ=255)                                                                                                                                                                                                                                  tAATGATCCAGAAGGTGCGGACGTCTTTTTTAAAGGTATAGATCCCTTCCCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAACCCGGCAACCCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGATATTCACGAAAAAGATCACCCAACGATTCTGGAAATGGCAAAAGCCg  <  2:88520/230‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                  ACTCTTACCGTTACTGTTTACCCCTGTGACAAAAGCCCGGACACCAGAAATGCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGATATTCACGAAAAAGATCACCCAACGATTCTGGAAATGGCAAAAGCCG  >  NC_000913/401773‑402227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑