Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 4,408,287 | A→C | Q545P (CAG→CCG) | rnr → | exoribonuclease R, RNase R |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,408,287 | 0 | A | C | 98.7% | 241.5 / ‑3.8 | 76 | Q545P (CAG→CCG) | rnr | exoribonuclease R, RNase R |
Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (34/41); total (35/41) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.61e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01 |
TATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAACTGGGTCAGCACTGTTCGATGGCGGAACGTCGT > NC_000913/4408054‑4408519 | tatCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCgg < 1:627617/236‑1 (MQ=255) tctcGGCCGCGCGTTTCGTTAAAAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGGCAAGCCGAGCACCGAAGCCATTCCCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCg < 2:199837/236‑1 (MQ=255) cggcggCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGCAGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAACGCTGCAAACCATGCTGCTGCGCTCGATGAAAACGGCGAtt > 1:529448/1‑236 (MQ=255) cggcgCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGGGACTACCCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACACTGCTGCTGCGCGCGATGAAACAGGCGCTTTa > 1:695860/1‑235 (MQ=255) ggcgCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTAc > 1:330492/1‑235 (MQ=255) cgcgTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGAt > 2:767291/1‑236 (MQ=255) cgcgTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGAACGTCCTGATGCAGAAATGCTGCAAACCAAGACGCATCGCTCGCTTGAACCGGCGATTTAAGag > 2:680988/1‑235 (MQ=255) tGAGAAAGCGAAAGATCCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAAcc < 1:519344/236‑1 (MQ=255) aGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTc > 1:38244/1‑236 (MQ=255) cACTGTGCCGTTGTCACGACAAGCCGAGCACGGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTgg < 2:529448/234‑1 (MQ=255) gTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGc > 1:511461/1‑231 (MQ=255) gTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGc > 1:483658/1‑231 (MQ=255) gTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTg < 1:20296/235‑1 (MQ=255) gTTCCGTATCCACGGCAAGCCGAGCAACGAAGCGATTCCCTAGTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTg < 2:184556/235‑1 (MQ=255) tCACGACAAGCCGAGCACCGAAGCGATTACCTC‑TTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATg > 1:369933/1‑235 (MQ=255) aCGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATgcg < 2:18096/236‑1 (MQ=255) gACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCa < 1:767291/236‑1 (MQ=255) aGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTgg < 1:467550/208‑1 (MQ=255) aaGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTAAGATCCAGAAAACCGTGGTCACTTTTGGCCGGCATTGCa > 2:467550/1‑208 (MQ=255) tttCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAg < 1:327828/236‑1 (MQ=255) ccGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGAcc < 1:130217/236‑1 (MQ=255) ccGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGAcc < 1:580567/236‑1 (MQ=255) ttCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCTAGGGGACTTTGGCCTGGGATTGCAGTCCCATGCGCACTTTAATTACCCGCTTCTTCGTAATccgagccgga > 2:145300/1‑228 (MQ=255) ttCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGCGGTCCCTTTGGCCTGGCATTTCAGTCCTATGCGCACCTTACTTCGCCGATTTGCCGTTAttccaaccta > 2:599899/1‑226 (MQ=255) cAGTGCTGGCGTAGCTGGGGCTGGACCTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACg < 1:185228/236‑1 (MQ=255) gCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGTGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGCAACCGGCGATTTa > 2:702312/1‑140 (MQ=255) gCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTa < 1:702312/140‑1 (MQ=255) gCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATtcgtcg < 2:16568/212‑1 (MQ=255) gCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATtcgtcg > 1:16568/1‑212 (MQ=255) ccgAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACcg < 1:384019/233‑1 (MQ=255) cGGAGTGGGGGCTGGAACTGCCGGGCGGTACCAAGCCGGACCCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACcg < 2:244767/235‑1 (MQ=255) aGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCgcgc < 2:190079/235‑1 (MQ=255) gCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTCATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGCTATTCCGACCTGACGCTGCACCGCGCCa > 2:73383/1‑236 (MQ=255) aaCTGCCGGGCGGTAACAAGCCGGAACCGCGTGCCTACGCGGAGCTGCTGGCGTCGGTTGGCGATCGTCCTGATGCAGAAATGCAGCAAACCATGCTTCTCCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCCCTTTGGCCTGGCATTGCATTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGCGGCTGCACCGCGCCATTTAata > 2:179693/1‑232 (MQ=255) aCAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTCATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCa < 1:73383/235‑1 (MQ=255) aaGCCGGAACCGCGTGACTACGGGGAGCTGCGGGAGTCGGTTCCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAgg < 2:115757/235‑1 (MQ=255) aaGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAgg < 1:196894/235‑1 (MQ=255) gAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAACATTCTGCTCGCGAAAGAGCAAGGGGAtaa > 2:499212/1‑234 (MQ=255) gAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAgg < 2:483658/229‑1 (MQ=255) gAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATc > 1:241936/1‑235 (MQ=255) ccGGGTGACTACGCGGAGCGGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCAt < 2:697687/231‑1 (MQ=255) cgTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCa > 2:18868/1‑235 (MQ=255) cgTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACACTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAAGGGCATcctggga > 2:258661/1‑229 (MQ=255) cgTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCCATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAACAGCAGGTGCATCAGGGCa > 2:150768/1‑235 (MQ=255) tGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAaca < 1:12705/236‑1 (MQ=255) cgcgGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACt > 1:433009/1‑235 (MQ=255) cgcgGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACt > 2:11158/1‑235 (MQ=255) ctgGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGc > 1:53403/1‑202 (MQ=255) ctgGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACGTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGc < 2:53403/202‑1 (MQ=255) tgGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCggcggc < 1:18868/236‑1 (MQ=255) ggAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCggcggc < 2:241936/235‑1 (MQ=255) gTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCggcggc > 2:515393/1‑232 (MQ=255) ggTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCat > 1:436854/1‑235 (MQ=255) gTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGCCGGCTACCatta > 1:178694/1‑236 (MQ=255) cGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCg < 1:11158/235‑1 (MQ=255) tCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCa > 1:47316/1‑222 (MQ=255) tCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCa < 2:47316/222‑1 (MQ=255) aaaTGCTGCAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGCGGCATCAGGGCAACACCACTGAAACCGGCGGCTCCCATTATTCGCTGGAagcaaggct > 2:559224/1‑225 (MQ=255) aaTGCTGCAAGCCATGCTGCTTCGCTTGAGGAAACCGGCGATTTCCGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAAc < 2:264616/236‑1 (MQ=255) gctgcAATCCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAAc < 1:145300/233‑1 (MQ=255) gctgcAAGCCATGCTGCTGCGCTTGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAAc < 1:258661/233‑1 (MQ=255) gctgcAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAAc < 2:38244/233‑1 (MQ=255) gctgcAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAAc < 1:515393/233‑1 (MQ=255) gctgcAAACCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAAc < 1:599899/233‑1 (MQ=255) tgcAACCCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAACTGGGt < 2:436854/236‑1 (MQ=255) aCCATGCTGCTGCGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAACTGGGTCAGCa < 1:680988/236‑1 (MQ=255) tgctgctgcGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATtcgtc < 1:429384/102‑1 (MQ=255) tgctgctgcGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATtcgtc > 2:429384/1‑102 (MQ=255) tgctgctgcGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGCGCAGGGGCATCAGGGCAACACCAGGTAAACCGGCGGCTACCATTATTCGATGGACAAGATGCTGGAACTggtctagaacggt > 2:431076/1‑225 (MQ=255) ctgctgcGCTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAACTGGGTCAGCCCTGTTc > 2:194829/1‑236 (MQ=255) gctgcGCTCGATGAAACCGGCGATTTACGATCCAGAAACCCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAACTGGGTCAGCACTGTTCGa < 2:351457/236‑1 (MQ=255) cgcTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCAt > 1:289187/1‑156 (MQ=255) cgcTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCAt < 2:289187/156‑1 (MQ=255) cgcTCGATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAACTGGGTCAGCACTGTTCGATGGc > 1:543415/1‑236 (MQ=255) gATGAAACCGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAACTGGGTCAGCACTGTTCGATGGCGGAAc > 2:515524/1‑236 (MQ=255) aaCCGGCGATTTACGATCCAGAAACCCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAACTGGGTCAGCACTGTTCGATGGCGGAAcgtcgt < 2:330492/236‑1 (MQ=255) | TATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGAACTGCCGGGCGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGCAACTGGGTCAGCACTGTTCGATGGCGGAACGTCGT > NC_000913/4408054‑4408519 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |