Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,475,797 A→C I121L (ATT→CTT)  yjgL → SopA‑central‑domain‑like hexapeptide repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,475,7970AC100.0% 100.3 / NA 28I121L (ATT→CTT) yjgLSopA‑central‑domain‑like hexapeptide repeat protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/15);  total (13/15)

AGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATC  >  NC_000913/4475575‑4476004
                                                                                                                                                                                                                              |                                                                                                                                                                                                               
aGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCAc                                                                                                                                                                                                    <  2:79349/236‑1 (MQ=255)
 ggAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACt                                                                                                                                                                                                   >  2:175235/1‑236 (MQ=255)
   aaTATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTAt                                                                                                                                                                                                 <  1:76527/236‑1 (MQ=255)
                  ggAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGAc                                                                                                                                                                                  >  1:639599/1‑236 (MQ=255)
                       aTTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGt                                                                                                                                                                              <  2:639599/235‑1 (MQ=255)
                          tttAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAg                                                                                                                                                                          >  2:395927/1‑236 (MQ=255)
                                                   aCCCTGGGTTATGTGGATTTTAGCAAACGCTCAAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCcaca                                                                                                                                                 <  1:295758/236‑1 (MQ=255)
                                                              tgtgGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTcc                                                                                                                                                                                                      <  1:249533/172‑1 (MQ=255)
                                                              tgtgGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTcc                                                                                                                                                                                                      >  2:249533/1‑172 (MQ=255)
                                                              tgtgGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCact                                                                                                                                                                                             <  1:241843/181‑1 (MQ=255)
                                                              tgtgGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCact                                                                                                                                                                                             >  2:241843/1‑181 (MQ=255)
                                                                                                                          cTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGa                                                                          >  1:3872/1‑236 (MQ=255)
                                                                                                                             aTCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTa                                                                       >  1:520899/1‑236 (MQ=255)
                                                                                                                                 tATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTaa                                                                   <  2:520899/236‑1 (MQ=255)
                                                                                                                                          gATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCt                                                          <  1:175235/236‑1 (MQ=255)
                                                                                                                                              cTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCg                                                      <  2:3872/236‑1 (MQ=255)
                                                                                                                                                                             gAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATg                                                                                                                       >  1:629964/1‑140 (MQ=255)
                                                                                                                                                                             gAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATg                                                                                                                       <  2:629964/140‑1 (MQ=255)
                                                                                                                                                                               ataatCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGtt                                                            <  1:338571/197‑1 (MQ=255)
                                                                                                                                                                               ataatCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGtt                                                            >  2:338571/1‑197 (MQ=255)
                                                                                                                                                                                        tAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCt                                                                                                                                             >  2:545528/1‑107 (MQ=255)
                                                                                                                                                                                        tAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCt                                                                                                                                             <  1:545528/107‑1 (MQ=255)
                                                                                                                                                                                                           aCCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAg                       >  2:636318/1‑206 (MQ=255)
                                                                                                                                                                                                           aCCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAg                       <  1:636318/206‑1 (MQ=255)
                                                                                                                                                                                                                 ggTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCgtg                                                    >  2:620350/1‑171 (MQ=255)
                                                                                                                                                                                                                 ggTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCgtg                                                    <  1:620350/171‑1 (MQ=255)
                                                                                                                                                                                                                 ggTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATc  <  1:408839/221‑1 (MQ=255)
                                                                                                                                                                                                                 ggTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATc  >  2:408839/1‑221 (MQ=255)
                                                                                                                                                                                                                              |                                                                                                                                                                                                               
AGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATC  >  NC_000913/4475575‑4476004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: