Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,613,561 C→T R27R (CGC→CGT yjjV → putative DNase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,613,5610CT98.5% 245.3 / NA 68R27R (CGC→CGTyjjVputative DNase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (32/35);  minor base G (1/0);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

AAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGATCGGTCTGGAT  >  NC_000913/4613330‑4613783
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                              
aaaaaGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACTGGCgg                                                                                                                                                                                                                            >  1:6568/1‑236 (MQ=255)
aaaaaGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCgg                                                                                                                                                                                                                            >  2:377686/1‑236 (MQ=255)
        ccgcTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTGCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCTGACCAAGCg                                                                                                                                                                                                                    >  2:297885/1‑236 (MQ=255)
                     aTCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAATAGGCCAGCCTGCAACTTTCGGCACCAGCGTGCGTAgggacaa                                                                                                                                                                                                       >  2:732354/1‑231 (MQ=255)
                          gggCCAGTGGTGACGGCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCAtt                                                                                                                                                                                                  <  1:397657/234‑1 (MQ=255)
                              cAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTc                                                                                                                                                                                              >  1:659135/1‑236 (MQ=255)
                                gtggtgACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCg                                                                                                                                                                                            >  1:41322/1‑236 (MQ=255)
                                                      tCATTCCGCTCGCCCCGGCCCCCAACTGTGCGCTGGTTGCAGAAGGAGGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGc                                                                                                                                                                     <  2:757337/236‑1 (MQ=255)
                                                       cATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGc                                                                                                                                                                                                           >  2:287214/1‑198 (MQ=255)
                                                       cATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGc                                                                                                                                                                                                           <  1:287214/198‑1 (MQ=255)
                                                         ttCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCCCAAGCGGGCgaa                                                                                                                                                                                                              >  1:167311/1‑191 (MQ=255)
                                                           ccGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCCCAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGc                                                                                                                                                                     >  1:168392/1‑232 (MQ=255)
                                                                  cgcCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGGAGGCAAGATCATTGTTCCGGCAACAGAGGCGGGAAAATTTGCCCCTGGGTTgg                                                                                                                                                          >  2:347607/1‑236 (MQ=255)
                                                                        tCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCACAAGAGGCGGAAAAATTTGCCCGGGGGTTGGCATTat                                                                                                                                                    >  2:137524/1‑235 (MQ=255)
                                                                          gcaagagttACTCTGGTTGCCGAAGTGGCCGAGCATCCGCAGGCAAACGACCCGACATTTAACAATGAGGATTTTGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCg                                                                                                                                                  <  2:143497/228‑1 (MQ=255)
                                                                                          ttcccaaggTGAGCGAAGCTCGGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACCCCCCCTGCCATTTTGGTTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCg                                                                                                                                   <  2:747634/227‑1 (MQ=255)
                                                                                              cGAAGTGAGCGAGCCTCCGCAGCCGAACGCCCCGACTTTTAACAATGAGGTTCTGGCTTGATTTGCCGTTTTATCGACCCCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTa                                                                                                                              <  2:206934/236‑1 (MQ=255)
                                                                                                 aGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATg                                                                                                                            <  1:193638/235‑1 (MQ=255)
                                                                                                 aGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATg                                                                                                                            <  2:41322/235‑1 (MQ=255)
                                                                                                     aGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGGAAAATTTGCCCGGGTGTTGGCATTAGCGGTAAATTATTAACAGCAGGATGCTGCa                                                                                                                       >  2:665354/1‑236 (MQ=255)
                                                                                                      gCGACCCTCCGCAGGCGAACGCCCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCAt                                                                                                                      <  1:54153/236‑1 (MQ=255)
                                                                                                          cGCTCCGCAGGCGAACGACCCGACATCTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGGGCGTTGGCATTAGCGGGACATTATCAAACGCTGTATGcccgactggg                                                                                                                  >  2:62717/1‑228 (MQ=255)
                                                                                                            cTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGc                                                                                                                 <  1:377686/235‑1 (MQ=255)
                                                                                                            cTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCt                                                                                                                >  1:279904/1‑236 (MQ=255)
                                                                                                               cGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTa                                                                                                                                                                                                              >  2:504871/1‑139 (MQ=255)
                                                                                                               cGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTa                                                                                                                                                                                                              <  1:504871/139‑1 (MQ=255)
                                                                                                                gaagtcGAACGTCCCGCAATTTAACAATGAGGATCTGGCTTGATTGGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCa                                                                                                            <  1:665354/231‑1 (MQ=255)
                                                                                                                     cGAACGACCCGACATTTAACAGTGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACGGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATc                                                                                                          <  1:763856/233‑1 (MQ=255)
                                                                                                                            cccGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGtt                                                                                                >  1:398649/1‑236 (MQ=255)
                                                                                                                              tgtacttaaaCAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACGGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTgg                                                                                              <  2:398649/228‑1 (MQ=255)
                                                                                                                              cGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCCGGATGCCGCATTGGGCTTGCATCCCGGTATGTTgg                                                                                              >  1:471349/1‑236 (MQ=255)
                                                                                                                                aCATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGaa                                                                                            <  1:551568/236‑1 (MQ=255)
                                                                                                                                aCATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGaa                                                                                            >  2:551568/1‑236 (MQ=255)
                                                                                                                                      aaCAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAg                                                                                                                                                    >  2:325145/1‑174 (MQ=255)
                                                                                                                                      aaCAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAg                                                                                                                                                    <  1:325145/174‑1 (MQ=255)
                                                                                                                                           tGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGa                                                                                 >  1:547358/1‑236 (MQ=255)
                                                                                                                                             aGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCaa                                                                                                                                      <  1:21361/181‑1 (MQ=255)
                                                                                                                                             aGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCaa                                                                                                                                      >  2:21361/1‑181 (MQ=255)
                                                                                                                                             aGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCCAACGCTGTTAGCCGCCTTTGGCTTGGATCCTGGTATGTTTGAAAAAAATAGCGac                                                                                >  2:614733/1‑234 (MQ=255)
                                                                                                                                              gtctCTGGCTTGATTGCCCGTTTTATCGACACCCACTGCCATTTGGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGAtgt                                                                              <  2:21865/233‑1 (MQ=255)
                                                                                                                                               gATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCTTTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGa                                                                                 <  1:346748/232‑1 (MQ=255)
                                                                                                                                               attCTGGTTGGATTGGCCTTTATACGCACCCCCTCGGCCATGTTGATTGCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGa                                                                                 <  2:352894/230‑1 (MQ=255)
                                                                                                                                                       ttGATTTGCCGTTTCAGCGACACCCACTGCCATTTGGATTCCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGa                                                                     <  2:547358/236‑1 (MQ=255)
                                                                                                                                                        tGATTTGCCGTTTAACCGACACCCACTGCCATTGTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGag                                                                    <  1:601403/236‑1 (MQ=255)
                                                                                                                                                        tGATTGGCCGTTTTACCGACGCCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGag                                                                    <  2:168392/236‑1 (MQ=255)
                                                                                                                                                         attttGCCTTTTTACCGCCCCCAACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGACATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGag                                                                    <  2:522408/233‑1 (MQ=255)
                                                                                                                                                          aTTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGACATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGagca                                                                  >  1:522408/1‑236 (MQ=255)
                                                                                                                                                                   tatATCGACCCCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTAcagca                                                         <  1:124963/234‑1 (MQ=255)
                                                                                                                                                                            acCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGa                                                >  2:36469/1‑236 (MQ=255)
                                                                                                                                                                                 cTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGaaaa                                                                                                                                             <  1:26633/138‑1 (MQ=255)
                                                                                                                                                                                 cTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGaaaa                                                                                                                                             >  2:26633/1‑138 (MQ=255)
                                                                                                                                                                                 cTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAgg                                            >  1:640472/1‑235 (MQ=255)
                                                                                                                                                                                 cTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGGATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGGGTCTCTTGAGCAGCTACAGCAGGGGCTGGAAAgga                                           >  1:371373/1‑235 (MQ=255)
                                                                                                                                                                                         tggATTACCCGCCGTTTAGGGCCGATGAAGGGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCg                                   <  2:535732/234‑1 (MQ=255)
                                                                                                                                                                                         tgaactccccGCGGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCg                                   <  2:553174/229‑1 (MQ=255)
                                                                                                                                                                                         gtGATTGCCCGCCGGTGAGTGGCGATGAAGGAGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCg                                   <  2:312506/235‑1 (MQ=255)
                                                                                                                                                                                            aTTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAg                                                                                                                                                                                                                      <  1:31419/54‑1 (MQ=255)
                                                                                                                                                                                            aTTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAg                                                                                                                                                                                                                      >  2:31419/1‑54 (MQ=255)
                                                                                                                                                                                              ttCCCGCCGTTTAGTGGCGATGAAGCGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGGGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTTGGCTTGCATCCTGGTATGTTGGGAAAACATAGCGCTGTGTCCCATTAGCAGCTACAGAAGGCGCTGGAAAGGCTTcatgcgcagct                              >  2:207144/1‑226 (MQ=255)
                                                                                                                                                                                                 ccgccgTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCGAAggtggt                           >  1:436503/1‑236 (MQ=255)
                                                                                                                                                                                                                gATGAAAGGGCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGAt            <  1:302214/236‑1 (MQ=255)
                                                                                                                                                                                                                 aTGAAGGCGCCAGCCGGCAACGTGCGGCACAAGGGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGAt            <  2:6568/235‑1 (MQ=255)
                                                                                                                                                                                                                         gCCAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCGAAGGTGGGGGCGGCGGGGgcgaatggaccggg   >  2:20083/1‑226 (MQ=255)
                                                                                                                                                                                                                           cAGCCTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGATCGGTCTGGAt  <  1:137524/235‑1 (MQ=255)
                                                                                                                                                                                                                              ccTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATc                                                                                                                                        <  1:223808/98‑1 (MQ=255)
                                                                                                                                                                                                                              ccTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATc                                                                                                                                        >  2:223808/1‑98 (MQ=255)
                                                                                                                                                                                                                              ccTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGAt            <  2:314323/222‑1 (MQ=255)
                                                                                                                                                                                                                              ccTGCAACGTGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGAt            >  1:314323/1‑222 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                              
AAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGATCGGTCTGGAT  >  NC_000913/4613330‑4613783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: