Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 623,458 T→G L387V (TTG→GTG)  entS → enterobactin exporter, iron‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913623,4580TG100.0% 233.5 / NA 69L387V (TTG→GTG) entSenterobactin exporter, iron‑regulated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/44);  total (25/44)

TGTGGATTTTAGGCGTGGTTTGTCTGGCGCTGTTCGGCTGGTTGAGTGCGGTCAGCTCGTTGCTGCAATACACAATGCTGCAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATT  >  NC_000913/623228‑623679
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                             
tgtgGATTTTAGGCGTGGTTTGTCTGGCGCTGTTCGGCTGGTTGAGTGCGGTCAGCTCGTTGCTGCAATACACAATGCTGCAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTgtgtt                                                                                                                                                                                                                           <  2:341601/235‑1 (MQ=255)
          aGGCTTGGTTTGTCTGGCGCTGTTCGGCTGGTTGAGTGCGGTCAGCTCGTTGCTGCAATACACAATGCTGCAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCg                                                                                                                                                                                                                <  2:513855/236‑1 (MQ=255)
          aGGCGTGGTTTGTCTGGCGCTGTTCGGCTGGTTGAGTGCGGTCAGCTCGTTGCTGCAATACACAATGCTGCAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGGTTTGGTGGGTTGATTATCGGCg                                                                                                                                                                                                                >  1:558443/1‑236 (MQ=255)
            gCGTGGTTTGTCTGGCGCTGTTCGGCTGGTTGAGTGCGGTCAGCTCGTTGCTGCAATACACAATGCTGCAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCgtg                                                                                                                                                                                                              <  2:472070/236‑1 (MQ=255)
            gCGTGGTTTGTCTGGCGCTGTTCGGCTGGTTGAGTGCGGTCAGCTCGTTGCTGAAATACACAATGCTGCAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGTTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCgtg                                                                                                                                                                                                              <  1:715431/236‑1 (MQ=255)
              gttgTTTGTCAGGCTATGTTCGGCTTGTTGAGTGCGGTCAGCTCGTTGCTGCAATACACAATGCTGCAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGtgtt                                                                                                                                                                                                            <  2:585203/233‑1 (MQ=255)
                                    gCTGGTTGAGTGCGGTCAGCTCGTTGCTGCAATACACAATGCTGCAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTGTTGGTGTGTTGATTATCCGCGGGTTGGTATTGCCTGTGCTGGGGGAg                                                                                                                                                                                      >  2:352271/1‑236 (MQ=255)
                                          tGAGTGCGGTCAGCTCGTTGCTGCAATACACAATGCTGCAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCAGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTAt                                                                                                                                                                                                                     <  2:325246/199‑1 (MQ=255)
                                          tGAGTGCGGTCAGCTCGTTGCTGCAATACACAATGCTGCAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCAGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTAt                                                                                                                                                                                                                     >  1:325246/1‑199 (MQ=255)
                                                            tgctgcAATACACAATGCTGCAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgccg                                                                                                                                                              <  1:594473/236‑1 (MQ=255)
                                                                    tACACAATGCTGCAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctggtg                                                                                                                                                                                               <  1:340321/195‑1 (MQ=255)
                                                                           tgctgcAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAAGCCGCTGCAGGGGACAGCg                                                                                                                                               >  1:165843/1‑236 (MQ=255)
                                                                           tgctgcAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGGGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGGGTGTTGATTATCGGCGTGTTGGTATTGCTGGTGCGGGTGGGGTTTGGACATTTTCGCCAGACTCCGCCGCAGTCGACAGCg                                                                                                                                               >  2:373468/1‑236 (MQ=255)
                                                                               gcacacgcaaccCACGGAAGAGATGTTAGGGCTGATTAACGGTTTGGAGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCg                                                                                                                                           <  2:558443/236‑1 (MQ=255)
                                                                                caaacggaaacCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTcc                                                                                                                                            <  1:737703/234‑1 (MQ=255)
                                                                                      caaccCCGGGAAGCGATGTTAGGGCGGATTAACGGTTGGTGGACGGCGCAGAACGTGACGGGCGTTGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTa                                                                                                                                    <  1:497707/236‑1 (MQ=255)
                                                                                                 agggATGTTAGGGCGGATTAACGGTTTTTGGCCGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAAc                                                                                                                         <  1:373468/233‑1 (MQ=255)
                                                                                                    gATGTTAGGGCGGATTAACGGTTTGGGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAg                                                                                                                       <  1:358197/235‑1 (MQ=255)
                                                                                                    gATGTAAGGCTGTATTGAGGGTGTGTGAACGGCACAGAGCGTGACGGGCGAGGCCATAGGCGCGGCGCTGCGGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAg                                                                                                                       <  2:384415/235‑1 (MQ=255)
                                                                                                     aTGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgccg                                                                                                                                                              <  1:555968/195‑1 (MQ=255)
                                                                                                     aTGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgccg                                                                                                                                                              >  2:555968/1‑195 (MQ=255)
                                                                                                         tAGGTCGGATTAACGGTTTGTGGGTGGCTCAGAACGTCACGGGCGATGCCGTAGGCGCGGCGGTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCt                                                                                                                  <  2:479782/235‑1 (MQ=255)
                                                                                                         tAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCt                                                                                                                  <  1:432945/235‑1 (MQ=255)
                                                                                                                gATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCCGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTa                                                                                                          >  1:159410/1‑236 (MQ=255)
                                                                                                                gATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGTCTGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTa                                                                                                          >  1:749309/1‑236 (MQ=255)
                                                                                                                gATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTa                                                                                                          >  1:529037/1‑236 (MQ=255)
                                                                                                                    aaCGGTTTGTGGACGGCGCAAAACGGTACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATcc                                                                                                       <  1:481753/235‑1 (MQ=255)
                                                                                                                    aaCGGTTTGGTGCCGGCGCAGCACCGGACGGGCGATGCCATAGGCGCGGACCTGCTGGGTGGTTTGGGCGGGATGATGACGCCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATcc                                                                                                       <  2:749309/235‑1 (MQ=255)
                                                                                                                           tgtgGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCg                                                                                                                                           <  1:216299/192‑1 (MQ=255)
                                                                                                                           tgtgGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCg                                                                                                                                           >  2:216299/1‑192 (MQ=255)
                                                                                                                             tgGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCa                                                                                             <  2:27259/236‑1 (MQ=255)
                                                                                                                                 cGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAACGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATgg                                                                                          <  2:704339/235‑1 (MQ=255)
                                                                                                                                   gcccAGAACGTGACGGGCGAGGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGgcg                                                                                        <  2:435523/232‑1 (MQ=255)
                                                                                                                                       aGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTa                                                                                                                                    <  1:741212/187‑1 (MQ=255)
                                                                                                                                        gAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGgcgc                                                                                       <  1:688584/231‑1 (MQ=255)
                                                                                                                                        gAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGgcgc                                                                                       <  2:594776/231‑1 (MQ=255)
                                                                                                                                         aaCGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgccgcc                                                                                                                                                            <  1:62814/161‑1 (MQ=255)
                                                                                                                                         aaCGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgccgcc                                                                                                                                                            >  2:62814/1‑161 (MQ=255)
                                                                                                                                         aaCGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTgtag                                                                                  >  1:698196/1‑235 (MQ=255)
                                                                                                                                             tGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATc                                                                             <  1:2254/236‑1 (MQ=255)
                                                                                                                                              gACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAAtgc                                                                            >  2:61823/1‑234 (MQ=255)
                                                                                                                                              gACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTAGCCAGACGCCGCCGCAGGGGACAGGGGCCGGCCGGTAATGCTTa                                                                                                                             >  2:741212/1‑187 (MQ=255)
                                                                                                                                               aCGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACACCGCCTTAAGCCTACCCAGCACTTGCATGGGCCTTAAGTAATCCa                                                                           >  1:256937/1‑236 (MQ=255)
                                                                                                                                                       tGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGaa                                                                    <  1:381661/235‑1 (MQ=255)
                                                                                                                                                                   ggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGc                                                                                                             >  2:605133/1‑182 (MQ=255)
                                                                                                                                                                   ggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGc                                                                                                             <  1:605133/182‑1 (MQ=255)
                                                                                                                                                                    gcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGGGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGGCCGCCAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCAATGCGCTGTAGCAATCCAGACGGAACGTCTAGGTTacc                                                       >  2:616491/1‑235 (MQ=255)
                                                                                                                                                                               tggtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCa                                                                                             >  2:5456/1‑186 (MQ=255)
                                                                                                                                                                               tggtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCa                                                                                             <  1:5456/186‑1 (MQ=255)
                                                                                                                                                                                ggtTTGGGCGCAATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAAcc                                           <  2:537244/235‑1 (MQ=255)
                                                                                                                                                                                   ttGGGCGCGATGATGACTCCGTTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGc                                        <  1:45104/235‑1 (MQ=255)
                                                                                                                                                                                   tgggacgcgATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGc                                        <  1:491415/230‑1 (MQ=255)
                                                                                                                                                                                   tggagagcgATGATGACTCCGGTTGCTTCCGCAAGCGCGAGCGGTTGTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGc                                        <  2:741172/229‑1 (MQ=255)
                                                                                                                                                                                    tGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCtt                                      >  1:443678/1‑236 (MQ=255)
                                                                                                                                                                                      ggCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTg                                     <  1:61823/235‑1 (MQ=255)
                                                                                                                                                                                       gcgcGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGa                                                                                                                                                    >  2:400292/1‑123 (MQ=255)
                                                                                                                                                                                       gcgcGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGa                                                                                                                                                    <  1:400292/123‑1 (MQ=255)
                                                                                                                                                                                           tatgagtacacCTGTTGCTTCCGCAACCGCGGGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTGATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTg                                <  2:415553/228‑1 (MQ=255)
                                                                                                                                                                                           gatgatGCCACCGGTTGCTTCCGCAAGGGCGAGCGGTTTTGGTGTGTTGATTAGCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTg                                <  2:715377/235‑1 (MQ=255)
                                                                                                                                                                                            atgatgACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGttt                                  >  2:579009/1‑232 (MQ=255)
                                                                                                                                                                                                        gTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTg                                                                                               <  1:149535/159‑1 (MQ=255)
                                                                                                                                                                                                        gTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTg                                                                                               >  2:149535/1‑159 (MQ=255)
                                                                                                                                                                                                              tCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGTGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGa            >  1:743185/1‑236 (MQ=255)
                                                                                                                                                                                                                cGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGag           <  1:458561/235‑1 (MQ=255)
                                                                                                                                                                                                                  cAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCa                                                                                             >  2:629170/1‑151 (MQ=255)
                                                                                                                                                                                                                  cAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCa                                                                                             <  1:629170/151‑1 (MQ=255)
                                                                                                                                                                                                                      ctcgggtggTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGa    <  2:257441/229‑1 (MQ=255)
                                                                                                                                                                                                                      cgcgAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCgg     >  1:405939/1‑235 (MQ=255)
                                                                                                                                                                                                                      cgcgAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGa    <  2:529037/236‑1 (MQ=255)
                                                                                                                                                                                                                          aGCGGTTTTGGTGTGTGGATTGTCGGCGTGGTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGAtt  <  1:352271/234‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                             
TGTGGATTTTAGGCGTGGTTTGTCTGGCGCTGTTCGGCTGGTTGAGTGCGGTCAGCTCGTTGCTGCAATACACAATGCTGCAAACGCAAACCCCGGAAGCGATGTTAGGGCGGATTAACGGTTTGTGGACGGCGCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATT  >  NC_000913/623228‑623679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: