Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	798,817	T→G	S78A (TCT→GCT)	pgl →	6‑phosphogluconolactonase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	798,817	0	T	G	100.0%	220.7 / NA	66	S78A (TCT→GCT)	pgl	6‑phosphogluconolactonase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (36/30);  total (36/30)

TGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTT  >  NC_000913/798587‑799047                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       tGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTgcg                                                                                                                                                                                                                                   <  1:539636/236‑1 (MQ=255)                 tatCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGt                                                                                                                                                                                                                       <  1:430906/232‑1 (MQ=255)                   tCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACg                                                                                                                                                                                                                 <  2:276684/236‑1 (MQ=255)                   tCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACg                                                                                                                                                                                                                 <  1:395160/236‑1 (MQ=255)                   gcGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACg                                                                                                                                                                                                                 <  1:390177/235‑1 (MQ=255)                     gCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGGGTTCGCCCTGAGTTTCGCGTCCTGGCGTACCGTATCGCCCCGGACGATGTCGCACTGACCTTTCCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCa                                                                                                                                                                                                               >  2:176428/1‑236 (MQ=255)                       cAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGTCGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGGATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGGCCGAGGCat                                                                                                                                                                                                              >  2:714833/1‑235 (MQ=255)                       cAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCat                                                                                                                                                                                                              >  1:111426/1‑235 (MQ=255)                               agagCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCACAttttacc                                                                                                                                                                                                      >  1:680280/1‑231 (MQ=255)                                    cAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCCGATTTTCACCGAtaa                                                                                                                                                                                                >  2:194054/1‑234 (MQ=255)                                    cAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGAtta                                                                                                                                                                                                >  2:175759/1‑234 (MQ=255)                                     aGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGAAGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCAAATTTTCACCGACCAc                                                                                                                                                                                               >  2:376998/1‑236 (MQ=255)                                                gTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGTGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTGGGCCGAGGCCTATTTCCGCAGATCACAAgggg                                                                                                                                                                                         >  2:504970/1‑231 (MQ=255)                                                          tGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTa                                                                                                                                                                          >  1:701583/1‑236 (MQ=255)                                                            aaTCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGCGGCGGCGCTGCCGGGTAGTCCGACGCCTATTTCCACCGAGCACCAGGGGCGGGTGGTCtttttc                                                                                                                                                                          >  2:727991/1‑231 (MQ=255)                                                            aaTCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCAGATTTCCACCGGTCACCAAGGGCCGGTTGTCTTTGTa                                                                                                                                                                          >  2:539845/1‑234 (MQ=255)                                                                aTGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAgg                                                                                                                                                                        <  1:194054/232‑1 (MQ=255)                                                                    aGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTg                                                                                                                                                                                                                                     <  2:281286/167‑1 (MQ=255)                                                                    aGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTg                                                                                                                                                                                                                                     >  1:281286/1‑167 (MQ=255)                                                                     tgcgcACTGACGCTGGCTCAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAGCGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACaa                                                                                                                                                               <  2:172222/235‑1 (MQ=255)                                                                                gCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGa                                                                                                                                                                                                   <  2:304791/189‑1 (MQ=255)                                                                                gCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGa                                                                                                                                                                                                   >  1:304791/1‑189 (MQ=255)                                                                                          gTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACCAACGTTATCTCTATGTTGGGGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGAAGTCCGACGCATATTTCTACCGATCACCACGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAAGGTGATCGTAAc                                                                                                                                          >  2:205946/1‑236 (MQ=255)                                                                                             gTCGATGTGCCGGGGCAGGTGCAGGAGATGGTGGTCAGCCCGGACAAACGTTATCTATATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTg                                                                                                                                                                            <  1:2645/199‑1 (MQ=255)                                                                                              tCGATGGGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTaa                                                                                                                                           <  1:376998/231‑1 (MQ=255)                                                                                                   gtgCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAgg                                                                                                                                                                        >  1:87476/1‑197 (MQ=255)                                                                                                   gtgCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCACGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAgg                                                                                                                                                                        <  2:87476/197‑1 (MQ=255)                                                                                                        ggggCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATg                                                                                                                            <  1:172730/236‑1 (MQ=255)                                                                                                                       gATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGc                                                                                                              <  2:196642/235‑1 (MQ=255)                                                                                                                         tggtggTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGc                                                                                                                          >  1:122590/1‑221 (MQ=255)                                                                                                                         tggtggTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGc                                                                                                                          <  2:122590/221‑1 (MQ=255)                                                                                                                               tCAGCCCGGACATACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGAt                                                                                                     >  1:94433/1‑236 (MQ=255)                                                                                                                                       gACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCg                                                                                              <  1:564233/235‑1 (MQ=255)                                                                                                                                           aaCGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATg                                                                                                                                                             <  1:382461/168‑1 (MQ=255)                                                                                                                                           aaCGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATg                                                                                                                                                             >  2:382461/1‑168 (MQ=255)                                                                                                                                              gtgaTCTCTATGTTGGTGTTCGCCCTGAGTTGCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTg                                                                                      <  1:727991/233‑1 (MQ=255)                                                                                                                                                       aTGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTg                                                                              >  2:223127/1‑235 (MQ=255)                                                                                                                                                        tGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGc                                                                             >  1:731371/1‑235 (MQ=255)                                                                                                                                                            ggTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCCGTGGGCGTCGTCGAGTTGGTCTAGGGGCTGGACGTTTGCGATTc                                                                        >  2:632601/1‑236 (MQ=255)                                                                                                                                                            ggTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGGCTGGAAGATGGCCTGCCAGTGGGCGTGGTCGAAGTGTTCGCGGGGCTGGACGGTTTCCAtt                                                                         >  2:578251/1‑235 (MQ=255)                                                                                                                                                             gtgtTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGc                                                                                                                          <  2:554528/185‑1 (MQ=255)                                                                                                                                                             gtgtTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGc                                                                                                                          >  1:554528/1‑185 (MQ=255)                                                                                                                                                                ttCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAg                                                                                            <  2:604584/212‑1 (MQ=255)                                                                                                                                                                ttCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAg                                                                                            >  1:604584/1‑212 (MQ=255)                                                                                                                                                                ttCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGcc                                                                    >  1:402961/1‑236 (MQ=255)                                                                                                                                                                  cGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCACTCGGCGTCGTCGATGTGGTCGCGTGGCTGGACGGTTGCCGtt                                                                         >  2:69729/1‑229 (MQ=255)                                                                                                                                                                  cGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGGAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGCCGATCTGGTCGAGGGGCGGGACGGTTGCCATTCTGCCa                                                                   >  2:154463/1‑235 (MQ=255)                                                                                                                                                                        gAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATAtct                                                             <  1:223127/235‑1 (MQ=255)                                                                                                                                                                              cgcgTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCgg                                                       >  1:700283/1‑235 (MQ=255)                                                                                                                                                                               gcgTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTCCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGa                                                      <  1:176428/235‑1 (MQ=255)                                                                                                                                                                                  tCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAAc                                                  <  1:175759/236‑1 (MQ=255)                                                                                                                                                                                     tGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCg                                                >  1:729104/1‑235 (MQ=255)                                                                                                                                                                                       gCGTATCGTATCGCCCCGAGCGATGGCCCACTGACCTTTGCCGCATAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTa                                              <  1:154463/235‑1 (MQ=255)                                                                                                                                                                                                 tcgCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCAATACGCCAATACTTCCCTGGACAActggtgcacgtg                                       >  2:712148/1‑221 (MQ=255)                                                                                                                                                                                                   gCCCGGGACGATGGCCCACTGACCTTGGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTcc                                 <  2:111426/236‑1 (MQ=255)                                                                                                                                                                                                         gACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGa                                                      >  2:79227/1‑209 (MQ=255)                                                                                                                                                                                                         gACGATGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGa                                                      <  1:79227/209‑1 (MQ=255)                                                                                                                                                                                                              tGGCGCACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCCATATCTCACCGGGAAACCGTACGCTTGGGTTTCCGGCATTaaat                       >  2:578369/1‑234 (MQ=255)                                                                                                                                                                                                                  gcACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGa                  <  2:700283/236‑1 (MQ=255)                                                                                                                                                                                                                   cACTGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCACTATCTCACCGGCCAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGaa                 >  1:383204/1‑235 (MQ=255)                                                                                                                                                                                                                      tGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTcc                                 <  2:448663/217‑1 (MQ=255)                                                                                                                                                                                                                      tGACCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTcc                                 >  1:448663/1‑217 (MQ=255)                                                                                                                                                                                                                        aCCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCTGCATTACAGACGGATCGCa             >  2:210327/1‑235 (MQ=255)                                                                                                                                                                                                                        aCCTTTGCCGCAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCAt            <  2:94433/236‑1 (MQ=255)                                                                                                                                                                                                                            tggCCGAAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTg         <  2:680280/233‑1 (MQ=255)                                                                                                                                                                                                                                  cAGAGGCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGGCTTAAAGAAGGACTGCATTTGCCTGttt  >  2:57614/1‑236 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       TGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTT  >  NC_000913/798587‑799047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑