Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 924,571 T→G I436M (ATT→ATG clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913924,5710TG97.6% 120.7 / ‑3.1 41I436M (ATT→ATGclpAATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (19/21);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01

AACCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATTCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCTTTCGAAAGCTTTGGG  >  NC_000913/924348‑924801
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        
aaCCGAAGTATGAAGCGCCCCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAagag                                                                                                                                                                                                                               <  1:204115/235‑1 (MQ=255)
aaCCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAgt                                                                                                                                                                                                                              <  2:3062/236‑1 (MQ=255)
   cGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGagaaga                                                                                                                                                                                                                                <  1:470319/231‑1 (MQ=255)
                     acgacgTGCGTTATACCGCAAAAGCGGTTCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGa                                                                                                                                                                                                          <  1:175739/235‑1 (MQ=255)
                            gCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTg                                                                                                                                                                                                           <  1:128233/227‑1 (MQ=255)
                                                 gcctgcgGCGGTAGAGCTGGCGGTGAAATACATTAACGGTCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCtt                                                                                                                                                                               <  1:664042/231‑1 (MQ=255)
                                                         cggTAAGGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATTCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCt                                                                                                                                                                     <  1:334199/235‑1 (MQ=255)
                                                                   ggCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCt                                                                                                                                                                                <  1:14279/215‑1 (MQ=255)
                                                                     cGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGGAATTGAGGTCCGGGGGGGCCGGATTTCACGCAAGCCAGAGAAGAGTGTTTCTCACAGAGGTCGTGGTAACCTGGAAAACCCCTGCGATAGCGTGTAAAATCCTgtctttgggccg                                                                                                                                                         >  2:27180/1‑225 (MQ=255)
                                                                              aCATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGGGGGGGCCCGGATTGCACGCATGCCCGAGAAGAGTGTTTCTCAGAGTGAGCGGGATACCCTGAAAAACCTCTGCGATCGCTTGTAAATGCTGGGCTTCGTGCAGGGTTAAgac                                                                                                                                               >  2:395790/1‑234 (MQ=255)
                                                                                aTTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGGGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGGAAATGCTGGTCTTCGGTCAGGATAAAGCCAt                                                                                                                                            >  1:542418/1‑236 (MQ=255)
                                                                                       aTCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCACAGAAGAGTGTTTTTCAGAGTGATCGTGATACCCTGTAAAACCCCGGCGATCGCTTGTAAATGCTGGGCTTCGGTCAGGGTACAGCCACTgt                                                                                                                                         >  2:77956/1‑231 (MQ=255)
                                                                                         cGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTTCACGCATGCCAGAGAAGAGTGGTTCTCAAAGTGATCGTGATACCCTGAAAAACCTCGGCGACCGCTTTACAATGCTGGTCTTCGGGCAGGATACAGCTATTGAgggg                                                                                                                                     >  2:544844/1‑232 (MQ=255)
                                                                                              tCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGGATTGCACGCATGGCCGAGAAAAGTGTTTTTCAGAGTGATCGTGATACCCCGAAAAAACACGCCGATCGCTTGTAAATGCCTGTCTTTGGGCAGGCTAAAGCCCATTAAGgcgcgacc                                                                                                                                >  2:284061/1‑231 (MQ=255)
                                                                                               cTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGctgactga                                                                                                                             <  1:395790/236‑1 (MQ=255)
                                                                                                               ttGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCCTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAAACTCGGCGATAGCTTGTAAATGCTGGGTTTCGGTCAGGATAAAGCCATTTAGGCGCTGCCTGAAGCCATTTAGATgtgg                                                                                                             >  2:32223/1‑233 (MQ=255)
                                                                                                                   tGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTg                                                                                                         <  1:83367/236‑1 (MQ=255)
                                                                                                                                       cTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACa                                                                                      <  1:284061/235‑1 (MQ=255)
                                                                                                                                         cgcgCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATa                                                                                    <  1:32223/235‑1 (MQ=255)
                                                                                                                                                            tAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGgttc                                                                       >  1:653925/1‑229 (MQ=255)
                                                                                                                                                                    cGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCgg                                                        <  2:653925/236‑1 (MQ=255)
                                                                                                                                                                               cGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGgcg                                                                                                                                     >  1:409597/1‑148 (MQ=255)
                                                                                                                                                                               cGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGgcg                                                                                                                                     <  2:409597/148‑1 (MQ=255)
                                                                                                                                                                                         gCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGt                                                                                                                                                           >  1:537573/1‑117 (MQ=255)
                                                                                                                                                                                         gCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGt                                                                                                                                                           <  2:537573/117‑1 (MQ=255)
                                                                                                                                                                                             ataattggctCCGTTGTCGCACGTATTGACCGCATGCCAGAGAGGAGGTTTTCTCAGAATGATCGTGATGCCCTGAAAAACCTCGGCGATCGCTTGAAAAGGCTGGTCTGCGGTCAGGATAAAGCCATTGAGGCGGTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGGTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACaga                                <  2:531244/227‑1 (MQ=255)
                                                                                                                                                                                                ttGAGTCCGTGGTGGCCCGTATTGCACGCATGCCAGATAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGt                             <  1:192548/235‑1 (MQ=255)
                                                                                                                                                                                                 tGAGTCAGTGTGGGCCCGTATTGCCCGCATGCAAGAGAGGATTGTTTCTCAGAGTGATCGTGATACCCTGAAAACCCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGa                           <  2:425777/236‑1 (MQ=255)
                                                                                                                                                                                                   aGTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGCTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACg                         >  2:187466/1‑236 (MQ=255)
                                                                                                                                                                                                     gTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGGTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAAAAAGGGTGACgg                        >  2:410116/1‑236 (MQ=255)
                                                                                                                                                                                                       ccGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTa                      <  1:77956/236‑1 (MQ=255)
                                                                                                                                                                                                           ggtggCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGATTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAg                   <  2:696509/235‑1 (MQ=255)
                                                                                                                                                                                                              ggCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAGAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCttt               >  1:388240/1‑236 (MQ=255)
                                                                                                                                                                                                              ggCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGGAACCCGAGGGGACGGTACAGCttt               >  2:630536/1‑236 (MQ=255)
                                                                                                                                                                                                              ggCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACATAGGGTAAGGTAACGCttt               >  2:143547/1‑236 (MQ=255)
                                                                                                                                                                                                                cccGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGCTGGGAAACCAGAGGTGACGGTACacacttcg             >  2:746190/1‑229 (MQ=255)
                                                                                                                                                                                                                 ccGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAGCCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCTTTCGa            <  1:143547/236‑1 (MQ=255)
                                                                                                                                                                                                                 ccGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCTTTCGa            <  1:630536/236‑1 (MQ=255)
                                                                                                                                                                                                                         cACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCTTTCGAAAGCTTTg    >  1:343357/1‑236 (MQ=255)
                                                                                                                                                                                                                           cGCATGCCAGAGAAGAGTGTTTCTCAGCGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGTCCGGGAAAACAGAGTGTCCGGTACAGCTTTCTACAGCTTTggg  >  1:376546/1‑236 (MQ=255)
                                                                                                                                                                                                                           cGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCTTTCGAAAGCTTTggg  >  1:750114/1‑236 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        
AACCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGAGTCCGTGGTGGCCCGTATTGCACGCATTCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTGAAAATGCTGGTCTTCGGTCAGGATAAAGCCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCTTTCGAAAGCTTTGGG  >  NC_000913/924348‑924801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: