Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,078,882	T→G	M1M (ATG→CTG) †	putA ←	fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,078,882	0	T	G	100.0%	220.9 / NA	59	M1M (ATG→CTG) †	putA	fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/33);  total (26/33)

GGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCATGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCAAAAAATGTGAGAG  >  NC_000913/1078653‑1079108                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   ggCTGGTGTGGTTCCTCTGCCGGAGGCGTGGCATCATCGCTCTCATTGGCCGCGCCAGAAAGCAGGGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCAt                                                                                                                                                                                                                               <  2:30965/235‑1 (MQ=255)  gCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCAtt                                                                                                                                                                                                                              >  2:61942/1‑235 (MQ=255)      gtgtGGTTCCGCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTc                                                                                                                                                                                                                          <  1:261280/235‑1 (MQ=255)                 ctGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGa                                                                                                                                                                                                              <  1:215303/236‑1 (MQ=255)                  tGCCGGAGTTGGTACTTAAGCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGaa                                                                                                                                                                                                             <  2:217729/236‑1 (MQ=255)                      ggAGTCGTGACTGCAGCGCTCCAATTGCCCGGCGCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGt                                                                                                                                                                                                         <  2:245865/236‑1 (MQ=255)                        aGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTg                                                                                                                                                                                                        <  1:74088/235‑1 (MQ=255)                            ggTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTCCTCCTGTTGTTCAGAAAGGTGCAAc                                                                                                                                                                                                    >  2:352702/1‑235 (MQ=255)                                    aTCGCTTTCAGTGGCCGCGCCAGAAAGCAGCGGAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGATGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTa                                                                                                                                                                                           <  2:452027/236‑1 (MQ=255)                                        ctctCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGt                                                                                                                                                                                       >  1:273409/1‑236 (MQ=255)                                         tctgATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAAATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTg                                                                                                                                                                                      <  1:547573/236‑1 (MQ=255)                                            cATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGaaa                                                                                                                                                                                   <  1:61942/236‑1 (MQ=255)                                                   cgcgCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTAc                                                                                                                                                                                                                            >  1:123838/1‑188 (MQ=255)                                                   cgcgCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTAc                                                                                                                                                                                                                            <  2:123838/188‑1 (MQ=255)                                                       ccAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTAATCGTGACAAATCCatgat                                                                                                                                                                         >  2:385795/1‑235 (MQ=255)                                                               agacgagtgTAGCTCGGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGc                                                                                                                                                                    <  2:555666/224‑1 (MQ=255)                                                                  cgcAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTtg                                                                                                                                                             >  1:690767/1‑236 (MQ=255)                                                                   gaaGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACt                                                                                                                                                                <  1:748092/230‑1 (MQ=255)                                                                      ggTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTtgtg                                                                                                                                                           <  1:470996/234‑1 (MQ=255)                                                                           cTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAAc                                                                                                                                                       <  1:385795/233‑1 (MQ=255)                                                                            tCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATg                                                                                                                                                   >  1:378554/1‑236 (MQ=255)                                                                               ggCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAACCCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGt                                                                                                                                                  <  1:67896/235‑1 (MQ=255)                                                                                 cAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGa                                                                                                                                                                                                              >  2:86567/1‑172 (MQ=255)                                                                                 cAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGa                                                                                                                                                                                                              <  1:86567/172‑1 (MQ=255)                                                                                  atagTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAAt                                                                                                                                             <  2:378554/234‑1 (MQ=255)                                                                                                ttCCAGTTGTTCGAGATAAGAAATAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGc                                                                                                                               <  2:671993/236‑1 (MQ=255)                                                                                                ttCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGc                                                                                                                               <  1:23275/236‑1 (MQ=255)                                                                                                           cGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTaaaaa                                                                                                                    <  2:690767/236‑1 (MQ=255)                                                                                                                        aaTCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATg                                                                                                         <  1:550999/234‑1 (MQ=255)                                                                                                                             ccTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACt                                                                                                                                                                                                   <  2:22508/139‑1 (MQ=255)                                                                                                                             ccTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACt                                                                                                                                                                                                   >  1:22508/1‑139 (MQ=255)                                                                                                                              cTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATaaa                                                                                                 >  1:99530/1‑236 (MQ=255)                                                                                                                                 cTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAg                                                                                               <  2:99530/235‑1 (MQ=255)                                                                                                                                    aattaaCCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAAt                                                                                           <  2:273409/236‑1 (MQ=255)                                                                                                                                    aagtaaCCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAAt                                                                                           <  1:730850/233‑1 (MQ=255)                                                                                                                                     attaaCCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAAt                                                                                           <  1:733167/235‑1 (MQ=255)                                                                                                                                     attaaCCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAAt                                                                                           <  1:462371/235‑1 (MQ=255)                                                                                                                                              tgtggtgtgCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACa                                                                                 >  1:278859/1‑236 (MQ=255)                                                                                                                                              tgtggtgtgCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACa                                                                                 >  1:483213/1‑236 (MQ=255)                                                                                                                                               gtggtgtaCTATCGTTCCGTGGCACGGCAGACGTAATCCGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGAc                                                                                  <  2:752395/234‑1 (MQ=255)                                                                                                                                                          tcgatACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTa                                                                                                                                                >  1:655755/1‑161 (MQ=255)                                                                                                                                                          tcgatACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTa                                                                                                                                                <  2:655755/161‑1 (MQ=255)                                                                                                                                                               aCGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAAc                                                                >  1:203349/1‑236 (MQ=255)                                                                                                                                                                    tCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAAc                                                                                                                                                       >  2:690527/1‑144 (MQ=255)                                                                                                                                                                    tCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAAc                                                                                                                                                       <  1:690527/144‑1 (MQ=255)                                                                                                                                                                    tCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACt                                                               <  2:5513/232‑1 (MQ=255)                                                                                                                                                                    tCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACt                                                               >  1:5513/1‑232 (MQ=255)                                                                                                                                                                                       cTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAaat                                                                                                                   <  2:548999/161‑1 (MQ=255)                                                                                                                                                                                       cTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAaat                                                                                                                   >  1:548999/1‑161 (MQ=255)                                                                                                                                                                                         cACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTaa                                      >  1:297437/1‑236 (MQ=255)                                                                                                                                                                                           cgcgtcgcgtCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACa                                    >  1:507205/1‑236 (MQ=255)                                                                                                                                                                                                                   ccccATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCaaa             <  2:170709/236‑1 (MQ=255)                                                                                                                                                                                                                      cATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCaaaaaa          >  1:547501/1‑236 (MQ=255)                                                                                                                                                                                                                      cATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGAAAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTACCATTTTTGAAAGGTGCAACCGCaacaaa          >  2:64184/1‑236 (MQ=255)                                                                                                                                                                                                                       aTGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCAAAAAAt         >  1:460998/1‑236 (MQ=255)                                                                                                                                                                                                                       aTGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAAAATTTTTGAAAGGTGCAACCGGCAAAAAt         >  2:568356/1‑236 (MQ=255)                                                                                                                                                                                                                             gtggtTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCAAAAAATGTgaga   >  2:516579/1‑236 (MQ=255)                                                                                                                                                                                                                             gtggtTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCAAAAAATGTgaga   >  2:524632/1‑236 (MQ=255)                                                                                                                                                                                                                              tggtTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCAAAAAATGTgagag  >  2:526597/1‑236 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   GGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCATGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCAAAAAATGTGAGAG  >  NC_000913/1078653‑1079108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑