Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,139,916 T→G D513E (GAT→GAG flgK → flagellar hook‑filament junction protein 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,139,9160TG100.0% 288.0 / NA 79D513E (GAT→GAGflgKflagellar hook‑filament junction protein 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/46);  total (33/46)

GAGCGAAGAAGATGCGGGTGATAGCGATAACCGCAACGGTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCC  >  NC_000913/1139685‑1140148
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        
gagcgaAGAAGATGCGGGTGATAGCGATAACCGCAACGGTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCgaggag                                                                                                                                                                                                                                       <  2:567317/235‑1 (MQ=255)
 agcgaAGAAGATGCGGGTGATAGCGATAACCGCAACGGTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGGGTCAATCTCGCggagga                                                                                                                                                                                                                                     >  1:638238/1‑236 (MQ=255)
     aagaagATGCGGGTGATAGCGATAACCGCAACGGTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTa                                                                                                                                                                                                                                  <  2:480014/235‑1 (MQ=255)
                ggTGATAGCGATAACCGCAACGGTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGGAATCTGCa                                                                                                                                                                                                                      >  2:567920/1‑236 (MQ=255)
                  ttaTAGCGATAACCGCAACGGTCAGGCCCTGCTGGATCGGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCa                                                                                                                                                                                                                      <  2:344386/232‑1 (MQ=255)
                  taaTAGCGATAACCGCAACGGTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCa                                                                                                                                                                                                                      <  1:507872/232‑1 (MQ=255)
                         gATAACCGCAACGGTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTca                                                                                                                                                                                                             <  1:115361/236‑1 (MQ=255)
                            aaCCGCAACGGTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGtt                                                                                                                                                                                                                 <  1:590817/229‑1 (MQ=255)
                                gCAACGGTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTAt                                                                                                                                                                                                      <  1:343369/236‑1 (MQ=255)
                                   aCGGTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTa                                                                                                                                                                                                       >  2:212146/1‑232 (MQ=255)
                                   aCGGTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTa                                                                                                                                                                                                       >  2:343369/1‑232 (MQ=255)
                                     ggTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCt                                                                                                                                                                                                 <  1:730127/236‑1 (MQ=255)
                                         aGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCgaggag                                                                                                                                                                                                                                       >  2:329090/1‑194 (MQ=255)
                                         aGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCgaggag                                                                                                                                                                                                                                       <  1:329090/194‑1 (MQ=255)
                                            cccTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGaa                                                                                                                                                                                           >  1:34845/1‑235 (MQ=255)
                                              ctgctgGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGtt                                                                                                                                                                                                                 <  1:291479/211‑1 (MQ=255)
                                              ctgctgGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCGGAATTACTTGCGGAATgg                                                                                                                                                                                        >  2:114889/1‑235 (MQ=255)
                                                   ggATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAgg                                                                                                                                                                                   <  1:212146/236‑1 (MQ=255)
                                                     aTCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTc                                                                                                                                                                                                              <  1:405283/207‑1 (MQ=255)
                                                     aTCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTc                                                                                                                                                                                                              >  2:405283/1‑207 (MQ=255)
                                                     aTCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGaa                                                                                                                                                                                           <  1:224968/226‑1 (MQ=255)
                                                     aTCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCa                                                                                                                                                                                     >  1:579373/1‑232 (MQ=255)
                                                     aTCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACATGGCGaa                                                                                                                                                                                           >  2:224968/1‑226 (MQ=255)
                                                     aTCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCCCGAGGAGGAGTACGGACATCTCTACCGTTTTcagcc                                                                                                                                                                                                          >  2:291479/1‑210 (MQ=255)
                                                              gCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACg                                                                                                                                                                        >  2:572368/1‑236 (MQ=255)
                                                                         aCGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAAcgc                                                                                                                                                                <  2:746736/233‑1 (MQ=255)
                                                                             tGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGAttttt                                                                                                                                                         >  2:97400/1‑236 (MQ=255)
                                                                               ggCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGa                                                                                                                                                       >  1:4615/1‑236 (MQ=255)
                                                                               ggCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATGTTTGa                                                                                                                                                       >  1:426499/1‑236 (MQ=255)
                                                                                    ttcaaaaTCCTTTAACGGCGCTTGGGCCTCGTTAGTGAGTGATATCGGTAATAAAGCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATgcg                                                                                                                                                   <  2:752908/231‑1 (MQ=255)
                                                                                    tGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAGCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATgcg                                                                                                                                                   <  1:668402/235‑1 (MQ=255)
                                                                                    tGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATgcg                                                                                                                                                   <  1:97400/235‑1 (MQ=255)
                                                                                         aaTCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGAt                                                                                                                                              <  2:4615/235‑1 (MQ=255)
                                                                                              tttAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACAt                                                                                                                                        <  1:243191/236‑1 (MQ=255)
                                                                                                tAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTc                                                                                                                                      >  2:172753/1‑236 (MQ=255)
                                                                                                   cgacgCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCt                                                                                                                                   <  1:172753/236‑1 (MQ=255)
                                                                                                      cgCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAg                                                                                                                                <  1:585780/236‑1 (MQ=255)
                                                                                                      cgCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAg                                                                                                                                >  1:699408/1‑236 (MQ=255)
                                                                                                      cgCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCACAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAg                                                                                                                                <  2:699408/236‑1 (MQ=255)
                                                                                                            tGCCTGTTTAGTTAGTGATGTCGGTAATAAAACCGGGACGTTGAAAACCAGTAGCCCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGAAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAggg                                                                                                                              <  2:318174/232‑1 (MQ=255)
                                                                                                                 cGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGa                                                                                                                     <  2:215838/236‑1 (MQ=255)
                                                                                                                     agtgagtgATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCg                                                                                                                 <  2:579373/236‑1 (MQ=255)
                                                                                                                     agtgagtgATAGCGGTAATAAACCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCg                                                                                                                 <  1:692596/236‑1 (MQ=255)
                                                                                                                     agtgagtaATATCGGTAATGAACCCGCGACGTTGAAAGCCAGTAGCGCCACGCAAGGTAATGAGGTGACCAAGTGTTCCAATCAGCAGCGGGTGAGTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCGGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCg                                                                                                                 <  2:340802/236‑1 (MQ=255)
                                                                                                                        gagtgaTATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGttt                                                                                                              <  2:715370/236‑1 (MQ=255)
                                                                                                                           tgaTATCGGTAATAAGACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCa                                                                                                            <  1:103294/235‑1 (MQ=255)
                                                                                                                           tgaTATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAg                                                                                                           >  2:368411/1‑236 (MQ=255)
                                                                                                                           tgaTATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAg                                                                                                           >  1:527906/1‑236 (MQ=255)
                                                                                                                              tatCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCg                                                                                                                 <  1:545766/227‑1 (MQ=255)
                                                                                                                              tatCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCg                                                                                                                 >  2:545766/1‑227 (MQ=255)
                                                                                                                               atCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTaca                                                                                                       <  1:114889/236‑1 (MQ=255)
                                                                                                                                tCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTcag                                                                                                                                                                                                            <  2:557678/134‑1 (MQ=255)
                                                                                                                                tCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTcag                                                                                                                                                                                                            >  1:557678/1‑134 (MQ=255)
                                                                                                                                     aataaAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGtt                                                                                                                                                                                                                 >  2:280372/1‑124 (MQ=255)
                                                                                                                                     aataaAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGtt                                                                                                                                                                                                                 <  1:280372/124‑1 (MQ=255)
                                                                                                                                     aataaAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCAGGTGACGATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGAGCAGGTTCTGCAGACGGCAAACGAGATATTTGATGAGCTGATTAACATTAGCTAAGGGGCGATCAGACGAGTTTCTGTACACAgata                                                                                                 >  2:102495/1‑235 (MQ=255)
                                                                                                                                        tgacccggtaCGTTGAAAACCAGTAGCGCCGCGTAAGGTAATGTGGTGACGCAGCTTGCCAATCAGCAGCAGTCGATTTCCGGTGGCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGGAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTAcaca                                                                                                     <  1:204923/220‑1 (MQ=255)
                                                                                                                                              gcgACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTATACAGATGATGTACCAg                                                                                        >  2:38358/1‑236 (MQ=255)
                                                                                                                                              gcgACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAg                                                                                        >  2:156392/1‑236 (MQ=255)
                                                                                                                                              gcgACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAg                                                                                        >  1:210050/1‑236 (MQ=255)
                                                                                                                                                         aaCCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCg                                                                             <  1:572368/236‑1 (MQ=255)
                                                                                                                                                                    ccACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTAcaca                                                                                                     >  1:258493/1‑201 (MQ=255)
                                                                                                                                                                    ccACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTAcaca                                                                                                     <  2:258493/201‑1 (MQ=255)
                                                                                                                                                                    ccACGCAAGGTAATGGGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCAcc                                                                   <  1:742877/235‑1 (MQ=255)
                                                                                                                                                                      aCGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACCaa                                                                 <  2:527906/235‑1 (MQ=255)
                                                                                                                                                                                      tgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGt                                                                            <  1:763139/208‑1 (MQ=255)
                                                                                                                                                                                      tgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGt                                                                            >  2:763139/1‑208 (MQ=255)
                                                                                                                                                                                      tgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACCaa                                                                 <  2:316666/219‑1 (MQ=255)
                                                                                                                                                                                      tgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACCaa                                                                 >  1:316666/1‑219 (MQ=255)
                                                                                                                                                                                       gACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACAt                                                                                                                                        >  1:169288/1‑147 (MQ=255)
                                                                                                                                                                                       gACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACAt                                                                                                                                        <  2:169288/147‑1 (MQ=255)
                                                                                                                                                                                        aCGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATg                                                                                                                                                     >  1:450479/1‑133 (MQ=255)
                                                                                                                                                                                        aCGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATg                                                                                                                                                     <  2:450479/133‑1 (MQ=255)
                                                                                                                                                                                        aCGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATg                                              >  1:337784/1‑236 (MQ=255)
                                                                                                                                                                                          gCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAATATTCGCTAAGGGGAGATAAGATGCGTTTCAGTacac                                                                                                      <  1:283777/178‑1 (MQ=255)
                                                                                                                                                                                          gCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAATATTCGCTAAGGGGAGATAAGATGCGTTTCAGTacac                                                                                                      >  2:283777/1‑178 (MQ=255)
                                                                                                                                                                                             gCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAga                                                                                                   >  1:727817/1‑178 (MQ=255)
                                                                                                                                                                                             gCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAga                                                                                                   <  2:727817/178‑1 (MQ=255)
                                                                                                                                                                                                        agcagcagTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTa                                         >  2:606021/1‑225 (MQ=255)
                                                                                                                                                                                                        agcagcagTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTa                                         <  1:606021/225‑1 (MQ=255)
                                                                                                                                                                                                                         ggTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGa             <  1:368411/236‑1 (MQ=255)
                                                                                                                                                                                                                                 tctcGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTaa     <  1:198726/236‑1 (MQ=255)
                                                                                                                                                                                                                                    cGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAAccc  <  1:156392/236‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        
GAGCGAAGAAGATGCGGGTGATAGCGATAACCGCAACGGTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGTAATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCC  >  NC_000913/1139685‑1140148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: